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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 田維誠(Wei-Cheng Tian) | |
dc.contributor.author | Pei-Shan Chung | en |
dc.contributor.author | 鍾佩珊 | zh_TW |
dc.date.accessioned | 2021-06-16T13:14:25Z | - |
dc.date.available | 2016-12-19 | |
dc.date.copyright | 2013-07-31 | |
dc.date.issued | 2013 | |
dc.date.submitted | 2013-07-29 | |
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Cleaning and Surface Activation of Microfabricated Interdigitated Microsensor Electrodes (IMEs), Planar Metal Electrodes (PMEs), Independently Addressable Microband Electrodes (IAMEs), and E’Chem “Cell-On-A-Chip”. Surface Cleaning Procedures [cited 2013; Available from: http://www.abtechsci.com/pdfs/clean0501.pdf. 60. Karnik, R., et al., Electrostatic control of ions and molecules in nanofluidic transistors. Nano Lett, 2005. 5(5): p. 943-8. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/61818 | - |
dc.description.abstract | 本研究開發的微奈米流體濃縮晶片是利用微米流道與奈米流道製成,施加一
電位差於具有離子選擇性(ion-selective)的奈米流道兩端可產生濃度極化效應(ion concentration polarization, ICP):於高電位處產生離子空乏區(ion depletion region),於低電位處產生離子濃縮區(ion concentration region)。而後,施加一偏壓於空乏區可產生第二種電滲透流(electroosmosis of second kind)並於空乏區的高電位處會產生原濃度的105~106 倍的濃縮區塊(preconcentration plug)。此濃縮器(preconcentrator)為樣本前處理步驟的一環並可整合於微全分析系統(micro total analysis system, μTAS),以大幅降低後端偵測的下限,達到低濃度生物標記(Biomarker)的偵測。 本文詳述微奈米流體濃縮晶片的設計與驗證流程: 濃縮晶片的流道設計與電路模擬、量測電路模擬所需參數、建立並驗證濃縮晶片的電阻模型以及驗證奈米流道具離子選擇性並求出啟動濃縮機制的最低操作電壓。 文獻中濃縮機制的啟動及濃縮區塊的行為多無法被預測與描述,因此本研究利用Labview架設迴路電流監測系統(loop currents monitoring system)描述離子於濃縮機制發生前後的現象:及時監測左、右迴路電流來判讀濃縮區塊出現的時間、位置以及濃縮區塊的行為模式。並利用迴路電流描述離子於濃縮機制發生後的電干擾現象、離子濃度與迴路電流的關係以及推導左右迴路電流不平均的電阻模型。其中,濃縮區塊的行為被歸類為以下四種模式:穩定濃縮模式(blocked mode)、濃縮區塊振盪模式(oscillation mode)、濃縮區塊突破強空乏區模式(burst mode)及濃縮區塊通過弱空乏區的離子槍模式(ion gun mode)。 本研究開發的迴路電流監測系統具有及時掌握濃縮機制啟動的時間以及濃縮區塊的行為模式與位置的能力,因此將來可採用免螢光標定(label-free)的檢體(analyte)、抗體(ligand)與試劑(reagent)等進行樣本預濃縮,以利後端的生物檢測。 | zh_TW |
dc.description.abstract | In this thesis, a novel method,”loop currents monitoring”, was developed to interpret the onset of the preconcentration mechanism and the behaviors of a preconcentrated plug of a nanofluidic preconcentrator without fluorescent labeling.
Initially, the design and validation methods and processes of nanofluidicpreconcentration chips were proposed starting from parameter setup, circuit simulations, developements and validations of resistive models, tests of ion-selective nanochannels to getting the lowest manipulation voltage of the onset of preconcentration mechanism. Four operational modes of a preconcentrated plug were observed during the preconcentration process and were explained by loop currents of the chip. The values of loop currents depended on how many counter-ions passing through cross-sectional areas of ion-selective nanochannels per second. The movements of the preconcentration plug and ion depletion region were described by flow profiles. Phenomena of uneven distribution of left and right loop currents and the relationship between gray values for fluorescent intensity of labeled proteins and magnitudes of loop currents after preconcentration mechanism happened were described in details. A resistive model after the preconcentration plug accured was constructed. In summary, the presented nanofluidic preconcentrator demonstrates multiple operational modes for a label-free protein preconcentration with the capability to precisely control and rapidly preconcentrate proteins. With the analysis of loop currents in our chip, various biological applications without fluorescent labeling could be demonstrated in the future. | en |
dc.description.provenance | Made available in DSpace on 2021-06-16T13:14:25Z (GMT). No. of bitstreams: 1 ntu-102-R00943075-1.pdf: 37630967 bytes, checksum: 9c8d51ce1425ee100f1f9a2c28c86b51 (MD5) Previous issue date: 2013 | en |
dc.description.tableofcontents | 口試委員定審書
...................................................................................................................... i 致謝 ........................................................................................................................................... ii 中文摘要 ................................................................................................................................. iii ABSTRACT ............................................................................................................................. iv 目錄 ......................................................................................................................................... vii 圖目錄 ....................................................................................................................................... x 表格目錄 ............................................................................................................................... xiii 第一章 導論 ............................................................................................................................ 1 1.1 生物晶片 ................................................................................................................................. 1 1.1.1 微全程分析系統技術(Micro Total Analysis System) ..................................................... 1 1.1.2 免疫分析法 ...................................................................................................................................... 2 1.1.3 生物分子相互作用分析法原理 .............................................................................................. 3 1.1.4 樣本前處理之預濃縮技術 ........................................................................................................ 6 1.2 微奈米流體濃縮晶片用於免疫分析 ............................................................................. 7 1.2.1 微奈米流體濃縮晶片之發展 ................................................................................................... 7 1.2.2 奈米流體晶片製程 ....................................................................................................................... 9 第二章 微奈米濃縮晶片用於免疫分析之原理 ....................................................... 11 2.1 微奈米流體濃縮原理 ...................................................................................................... 11 2.1.1 電雙層效應 ................................................................................................................................... 11 2.1.1.1 電雙層重疊效應 ................................................................................................................................. 14 2.1.2 離子區域性的空乏與濃縮現象 ........................................................................................... 14 2.1.3 離子選擇性薄膜之電壓與電流的S 曲線(I-V S-curve) ............................................. 18 2.1.3.1 不穩定的第二型電滲透流造成的漩渦狀流線之電干擾 ................................................... 20 2.1.4 電路模擬微奈米流體晶片與限電流計算 ........................................................................ 21 2.1.5 預濃縮機制 ................................................................................................................................... 26 2.2 微奈米濃縮晶片之免疫分析原理 ............................................................................... 27 viii 2.2.1 金的表面處理方法於免疫分析之用途 ............................................................................. 27 2.2.1.1 自組裝單分子層(SAM)與金表面離子鍵結 ............................................................................ 28 2.2.1.2 EDC/NHS 作為介面反應之媒介物 ............................................................................................. 30 2.2.1.3 抗體抗原接合原理 ............................................................................................................................ 32 第三章 微奈米流體濃縮晶片之設計與量測系統 ................................................... 33 3.1 微奈米流體濃縮晶片設計 ............................................................................................. 33 3.1.1 濃縮晶片設計與模擬 ............................................................................................................... 33 3.1.2 奈米流道的離子選擇性功能驗證(I-V S-curve) ............................................................ 36 3.1.3 濃縮晶片電路驗證之實驗 ..................................................................................................... 37 3.2 微奈米流體濃縮晶片之製作與驗證 .......................................................................... 44 3.2.1 製程方法 ....................................................................................................................................... 44 3.2.1.1 金膜圖案之製程 ................................................................................................................................. 44 3.2.1.2 蝕刻奈米流道之玻璃製程 ............................................................................................................. 45 3.2.1.3 微米流道之翻膜製程 ....................................................................................................................... 48 3.2.1.4 氧電漿接合製程 ................................................................................................................................. 50 3.2.1.5 免疫分析之流程 ................................................................................................................................. 52 3.3 微奈米流體濃縮晶片之量測系統 ............................................................................... 54 3.3.1 倒立式螢光顯微鏡量測系統 ................................................................................................ 54 3.3.2 LabView 架設迴路電流量測系統 ........................................................................................ 54 第四章 微奈米流體濃縮晶片結果與討論 ................................................................. 57 4.1 利用迴路電流量測微奈米流體濃縮晶片 ................................................................. 57 4.1.1 濃縮區塊多種行為模式 .......................................................................................................... 58 4.1.1.1 阻擋模式(Blocked mode) ................................................................................................................ 59 4.1.1.2 振盪模式(Oscillation mode) ........................................................................................................... 60 4.1.1.3 突破模式(Burst mode) ..................................................................................................................... 62 4.1.1.4 離子槍模式(Ion gun mode) ............................................................................................................ 66 4.1.2 利用迴路電流觀測濃縮機制之產生 .................................................................................. 67 4.1.2.1 濃縮區塊發生位置之驗證 ............................................................................................................. 70 4.1.2.2 濃縮啟動後強勢迴路電流與螢光強度的關係 ...................................................................... 71 4.1.2.3 濃縮啟動時間與電壓的關係 ........................................................................................................ 71 4.1.3 利用迴路電流描述濃縮區塊之行為 .................................................................................. 73 ix 4.1.3.1 迴路電流表現的再現性 .................................................................................................................. 76 4.2 濃縮區塊發生後的電阻模型推測 ............................................................................... 77 4.2.1 左右迴路電流大小不平均之推測 ....................................................................................... 77 4.2.2 不同濃度的PBS 緩衝溶液的電流電壓曲線 .................................................................. 79 4.3 微奈米流體濃縮晶片之免疫分析 ............................................................................... 81 4.3.1 金的表面修飾方法驗證 .......................................................................................................... 81 4.3.2 微奈米流體濃縮晶片用於免疫分析之流程 ................................................................... 84 第五章 總結與未來展望 ................................................................................................. 85 5.1 總結 ....................................................................................................................................... 85 5.2 未來展望 .............................................................................................................................. 86 參考文獻 ................................................................................................................................ 87 | |
dc.language.iso | zh-TW | |
dc.title | 利用電流特性觀察微奈米濃縮晶片之作用機制及其應用 | zh_TW |
dc.title | Development of Label-free Nanofluidic Preconcentration Chip
by Loop Currents Monitoring for Immunoassay Application | en |
dc.type | Thesis | |
dc.date.schoolyear | 101-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 宋孔彬(Kung-Bin Sun),沈弘俊(Horn-Jiunn Sheen),呂家榮(Lu Chia-Jung) | |
dc.subject.keyword | 免標定奈米流體樣本預濃縮,離子選擇性,濃度極化效應,迴路電流監測,電阻模型, | zh_TW |
dc.subject.keyword | label-free nanofluidic sample preconcentration,ion-selective nanochannel,ion concentration polarization (ICP),loop currents monitoring,resistive model, | en |
dc.relation.page | 93 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2013-07-30 | |
dc.contributor.author-college | 電機資訊學院 | zh_TW |
dc.contributor.author-dept | 電子工程學研究所 | zh_TW |
顯示於系所單位: | 電子工程學研究所 |
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