請用此 Handle URI 來引用此文件:
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/6126
完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 謝旭亮 | |
dc.contributor.author | Ming-Chieh Tsai | en |
dc.contributor.author | 蔡旻潔 | zh_TW |
dc.date.accessioned | 2021-05-16T16:21:29Z | - |
dc.date.available | 2018-08-06 | |
dc.date.available | 2021-05-16T16:21:29Z | - |
dc.date.copyright | 2013-08-06 | |
dc.date.issued | 2013 | |
dc.date.submitted | 2013-07-29 | |
dc.identifier.citation | Achard, P., and Genschik, P. (2009). Releasing the brakes of plant growth: How GAs shutdown DELLA proteins. J. Exp. Bot. 60: 1085-1092.
Alonso-Ramírez, A., Rodríguez, D., Reyes, D., Jiménez, J.A., Nicolás, G., López-Climent, M., Gómez-Cadenas, A., Nicolás, C. (2009). Evidence for a role of gibberellins in salicylic acid-modulated early plant responses to abiotic stress in Arabidopsis seeds. Plant Physiol. 150: 1335-44. Aubert, D., Chevillard, M., Dorne, A.M., Arlaud, G., and Herzog, M. (1998). Expression patterns of GASA genes in Arabidopsis thaliana: the GASA4 gene is up-regulated by gibberellins in meristematic regions. Plant Mol. Biol. 36: 871-883. Block, A., Schmelz, E., Jones, J.B., and Klee, H.J. (2005). Coronatine and salicylic acid: the battle between Arabidopsis and Pseudomonas for phytohormone control. Mol. Plant Pathol. 6: 79-83. Chaerle, L., Van Der Straeten, D. (2000). Imaging techniques and the early detection of plant stress. Trends in Plant Sci. 5: 495-501. Chen, I.C., Lee, S.C., Pan, S.M., and Hsieh, H.L. (2007a). GASA4, a GA-stimulated gene, participates in light signaling in Arabidopsis. Plant Sci. 172: 1062-1071. Chen, I.C., Huang, I.C., Liu, M.J., Wang, Z.G., Chung, S.S., and Hsieh, H.L. (2007b). Glutathione S-transferase interacting with far-red insensitive 219 is involved in phytochrome A-mediated signaling in Arabidopsis. Plant Physiol. 143: 1189-1202. Cheng, H., Qin, L., Lee, S., Fu, X., Richards, D.E., Cao, D., Luo, D., Harberd, N.P., Peng, J. (2004). Gibberellin regulates Arabidopsis floral development via suppression of DELLA protein function. Development 131: 1055-1064. Chini, A., Fonseca, S., Fernandez, G., Adie, B., Chico, J.M., Lorenzo, O., Garcia-Casado, G., Lopez-Vidriero, I., Lozano, F.M., Ponce, M.R., Micol, J.L., and Solano, R. (2007). The JAZ family of repressors is the missing link in jasmonate signalling. Nature 448: 666-671. Chitnis, P.R. (2001). PHOTOSYSTEM I: Function and Physiology. Annu. Rev. Plant Physiol. Plant Mol. Biol. 52: 593-626. Chollet, R., Vidal, J., and O'Leary, M.H. (1996) PHOSPHOENOLPYRUVATE CARBOXYLASE: a ubiquitous, highly regulated enzyme in plants. Annu. Rev. Plant Physiol. Plant Mol. Biol. 47: 273-298. Clough, S.J., and Bent, A.F. (1998). Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana. Plant J. 16: 735-743. Dekker, J.P., and Boekema, E.J. (2005). Supramolecular organization of thylakoid membrane proteins in green plants. Biochim. Biophys. Acta. 1706: 12-39. Desnos, T., Puente, P., Whitelam, G.C., and Harberd, N.P. (2001). FHY1: a phytochrome A-specific signal transducer. Genes Dev. 15: 2980-2990. Devoto, A., and Turner, J.G. (2003). Regulation of jasmonate-mediated plant responses in Arabidopsis. Ann. Bot. 92: 329-337. Devoto, A., Nieto-Rostro, M., Xie, D., Ellis, C., Harmston, R., Patrick, E., Davis, J., Sherratt, L., Coleman, M., and Turner, J.G. (2002). COI1 links jasmonate signalling and fertility to the SCF ubiquitin-ligase complex in Arabidopsis. Plant J. 32: 457-466. Dill, A., and Sun, T. (2001). Synergistic derepression of gibberellin signaling by removing RGA and GAI function in Arabidopsis thaliana. Genetics 159: 777–785. Fairchild, C.D., Schumaker, M.A., and Quail, P.H. (2000). HFR1 encodes an atypical bHLH protein that acts in phytochrome A signal transduction. Genes Dev. 14: 2377-2391. Fankhauser, C., and Chory, J. (2000). RSF1, an Arabidopsis locus implicated in phytochrome A signaling. Plant Physiol. 124: 39-45. Farmer, E.E., and Ryan, C.A. (1990). Interplant communication: airborne methyl jasmonate induces synthesis of proteinase inhibitors in plant leaves. Proc. Natl. Acad. Sci. USA 87: 7713-7716. Feng, S., Martinez, C., Gusmaroli, G., Wang, Y., Zhou, J., Wang, F., Chen, L., Yu, L., Iglesias-Pedraz, J.M., Kircher, S., Schafer, E., Fu, X., Fan, L.M., and Deng, X.W. (2008). Coordinated regulation of Arabidopsis thaliana development by light and gibberellins. Nature 451:475–479. Fonseca, S., Chini, A., Hamberg, M., Adie, B., Porzel, A., Kramell, R., Miersch, O., Wasternack, C., and Solano, R. (2009). (+)-7-iso-Jasmonoyl-L-isoleucine is the endogenous bioactive jasmonate. Nat. Chem. Biol. 5: 344-350. Fu, X., and Harberd, N.P. (2003). Auxin promotes Arabidopsis root growth by modulating gibberellin response. Nature 421: 740-743. Hoecker, U., Xu, Y., and Quail, P.H. (1998). SPA1: a new genetic locus involved in phytochrome A-specific signal transduction. Plant Cell 10: 19-33. Hoecker, U., Tepperman, J.M., and Quail, P.H. (1999). SPA1, a WD-repeat protein specific to phytochrome A signal transduction. Science 284: 496-499. Horton, P., Ruban, A.V., and Walters, R.G. (1996). Regulation of Light Harvesting in Green Plants. Annu. Rev. Plant Physiol. Plant Mol. Biol. 47: 655-684. Hou, X., Lee, L.Y.C., Xia, K., Yan, Y., and Yu, H. (2010). DELLAs modulate jasmonate signaling via competitive binding to JAZs. Dev. Cell 19: 884–894. Hou, X., Hu, W.W., Shen, L., Lee, L.Y., Tao, Z., Han, J.H., and Yu, H. (2008). Global identification of DELLA target genes during Arabidopsis flower development. Plant Physiol. 147: 1126-1142. Hsieh, H.L., Okamoto, H., Wang, M., Ang, L.H., Matsui, M., Goodman, M., and Deng, X.W. (2000). FIN219, an auxin-regulated gene, defines a genetic link between phytochrome A and the downstream regulator COP1 in light control of Arabidopsis development. Genes Dev. 14: 1958-1970. Jensen, P.E., Gilpin, M., Knoetzel, J., and Scheller, H.V. (2000). The PSI-K subunit of photosystem I is involved in the interaction between light-harvesting complex I and the photosystem I reaction center core. J. Biol. Chem. 275: 24701-24708. Katsir, L., Schilmiller, A.L., Staswick, P.E., He, S.Y., and Howe, G.A. (2008). COI1 is a critical component of a receptor for jasmonate and the bacterial virulence factor coronatine. Proc. Natl. Acad. Sci. USA 105: 7100-7105. Kim, J., Yi, H., Choi, G., Shin, B., and Song, P.S. (2003). Functional characterization of phytochrome interacting factor 3 in phytochrome-mediated light signal transduction. Plant Cell 15: 2399-2407. Kim, Y.M., Woo, J.C., Song, P.S., and Soh, M.S. (2002). HFR1, a phytochrome A-signalling component, acts in a separate pathway from HY5, downstream of COP1 in Arabidopsis thaliana. Plant J. 30: 711-719. Kiss, A.Z., Ruban, A.V. and Horton, P. (2008). The PsbS protein controls the organisation of the photosystem II antenna in higher plant thylakoid membranes. J. Biol. Chem. 283: 3972–3988. Ko, C.B., Woo, Y.M., Lee, D.J., Lee, M.C., and Kim, C.S. (2007). Enhanced tolerance to heat stress in transgenic plants expressing the GASA4 gene. Plant Physiol. Biochem. 45: 722-728. Krah, N.M., and Logan, B.A. (2010). Loss of psbS expression reduces vegetative growth, reproductive output, and light-limited, but not light-saturated, photosynthesis in Arabidopsis thaliana (Brassicaceae) grown in temperate light environments. Am. J. Bot. 97: 644-649. Lee, S., Cheng, H., King, K.E., Wang, W., He, Y., Hussain, A., Lo, J., Harberd, N.P., and Peng, J. (2002). Gibberellin regulates Arabidopsis seed germination via RGL2, a GAI/RGA-like gene whose expression is up-regulated following imbibition. Gene Dev. 16: 646-658. Li, K.L., Bai, X., Li, Y., Cai, H., Ji, W., Tang, L.L., Wen, Y.D., and Zhu, Y.M. (2011). GsGASA1 mediated root growth inhibition in response to chronic cold stress is marked by the accumulation of DELLAs. J. Plant Physiol. 168: 2153-2160. Melotto, M., Underwood, W., Koczan, J., Nomura, K., and He, S.Y. (2006). Plant stomata function in innate immunity against bacterial invasion. Cell 126: 969-980. Nahirñak, V., Almasia, N.I., Hopp, H.E., and Vazquez-Rovere, C. (2012) Snakin/GASA proteins: involvement in hormone crosstalk and redox homeostasis. Plant Signal Behav. 7: 1004-1008. Navarro, L., Bari, R., Achard, P., Lison, P., Nemri, A., Harberd, N.P., and Jones, J.D. (2008). DELLAs control plant immune responses by modulating the balance of jasmonic acid and salicylic acid signaling. Curr. Biol. 18: 650-655. Nishii, A., Takemura, M., Fujita, H., Shikata, M., Yokota, A., and Kohchi, T. (2000). Characterization of a novel gene encoding a putative single zinc-finger protein, ZIM, expressed during the reproductive phase in Arabidopsis thaliana. Biosci. Biotechnol. Biochem. 64: 1402-1409. Pauwels, L., Inze, D., and Goossens, A. (2009). Jasmonate-inducible gene: What does it mean? Trends Plant Sci. 14: 87-91. Porra, R.J., Thompson, W.A., and Kriedemann, P.E. (1989). Determination of accurate extinction coefficients and simultaneous equations for assaying chlorophylls a and b extracted with four different solvents: verification of the concentration of chlorophyll standards by atomic absorption spectroscopy. Biochim. Biophys. Acta 975: 384-394. Qi, T., Song, S., Ren, Q., Wu, D., Huang, H., Chen, Y., Fan, M., Peng, W., Ren, C., and Xie, D. (2011). The Jasmonate-ZIM-domain proteins interact with the WD-Repeat/bHLH/MYB complexes to regulate Jasmonate-mediated anthocyanin accumulation and trichome initiation in Arabidopsis thaliana. Plant Cell 23: 1795-1814. Quail, P. H. (1997). The phytochromes: a biochemical mechanism of signaling in sight? Bioassays 19: 571-579. Rubinovich, L., and Weiss, D. (2010). The Arabidopsis cysteine-rich protein GASA4 promotes GA responses and exhibits redox activity in bacteria and in planta. Plant J. 64: 1018-1027. Sakamoto, T., Miura, K., Itoh, H., Tatsumi, T., Ueguchi-Tanaka, M., Ishiyama, K., Kobayashi, M., Agrawal, G.K., Takeda, S., Abe, K., Miyao, A., Hirochika, H., Kitano, H., Ashikari, M., and Matsuoka, M. (2004). An overview of gibberellin metabolism enzyme genes and their related mutants in rice. Plant Physiol. 134: 1642-1653. Shi, L., Gast, R.T., Gopalraj, M., and Olszewski, N.E. (1992). Characterization of a shoot-specific, GA3- and ABA-regulated gene from tomato. Plant J. 2: 153-159. Smith, H. (2000). Phytochromes and light signal perception by plants-an emerging synthesis. Nature 407: 585-591. Song, S., Qi, T., Huang, H., Ren, Q., Wu, D., Chang, C., Peng, W., Liu, Y., Peng, J., and Xie, D. (2011). The Jasmonate-ZIM domain proteins interact with the R2R3-MYB transcription factors MYB21 and MYB24 to affect Jasmonate-regulated stamen development in Arabidopsis. Plant Cell 23: 1000-1013. Staswick, P.E. (2002). Jasmonate response locus JAR1 and several related Arabidopsis genes encode enzymes of the firefly luciferase superfamily that show activity on jasmonic, salicylic, and indole-3-acetic acids in an assay for adenylation. Plant Cell 14: 1405-1415. Sullivan, J.A., and Deng, X.W. (2003). From seed to seed: the role of photoreceptors in Arabidopsis development. Dev. Biol. 260: 289-297. Szabo, I., Bergantino, E., and Giacometti, G.M. (2005). Light and oxygenic photosynthesis: energy dissipation as a protection mechanism against photo-oxidation. EMBO Rep. 6: 629-634. Thines, B., Katsir, L., Melotto, M., Niu, Y., Mandaokar, A., Liu, G., Nomura, K., He, S.Y., Howe, G.A., and Browse, J. (2007). JAZ repressor proteins are targets of the SCF(COI1) complex during jasmonate signalling. Nature 448: 661-665. Tseng, T.S., Swain, S.M., and Olszewski, N.E. (2001). Ectopic expression of the tetratricopeptide repeat domain of SPINDLY causes defects in gibberellin response. Plant Physiol. 129: 605-615. Wang, L., Wang, Z., Xu, Y., Joo, S.H., Kim, S.K., Xue, Z., Xu, Z., Wang, Z., and Chong, K. (2009). OsGSR1 is involved in crosstalk between gibberellins and brassinosteroids in rice. Plant J. 57: 498-510 Wang, J.G., Chen, C.H., Chien, C.T. and Hsieh, H.L. (2011). FAR-RED INSENSITIVE 219 modulates CONSTITUTIVE PHOTOMORPHOGENIC 1 activity via physical interaction to regulate hypocotyl elongation in Arabidopsis. Plant Physiol. 156: 631-646. Wang, H., and Deng, X.W. (2002). Arabidopsis FHY3 defines a key phytochrome A signaling component directly interacting with its homologous partner FAR1. EMBO J. 21: 1339-1349. Wasternack, C. (2007). Jasmonates: an update on biosynthesis, signal transduction and action in plant stress response, growth and development. Ann. Bot. 100: 681-697. Wild, M., Daviere, J.M., Cheminant, S., Regnault, T., Baumberger, N., Heintz, D., Baltz, R., Genschik, P., and Achard, P. (2012). The Arabidopsis DELLA RGA-LIKE3 is a direct target of MYC2 and modulates jasmonate signaling responses. Plant Cell 24: 3307-3319. Xu, L., Liu, F., Lechner, E., Genschik, P., Crosby, W.L., Ma, H., Peng, W., Huang, D., and Xie, D. (2002). The SCF(COI1) ubiquitin-ligase complexes are required for jasmonate response in Arabidopsis. Plant Cell 14: 1919-1935. Yang, D.L., Yao, J., Mei, C.S., Tong, X.H., Zeng, L.J., Li, Q., Xiao, L.T., Sun, T.P., Li, J., Deng, X.W., Lee, C.M., Thomashow, M.F., Yang, Y., He, Z., and He, S.Y. (2012). Plant hormone jasmonate prioritizes defense over growth by interfering with gibberellin signaling cascade. Proc. Natl. Acad. Sci. USA 109: E1192-E1200. Yu, H., Ito, T., Zhao, Y., Peng, J., Kumar, P., and Meyerowitz, E.M. (2004). Floral homeotic genes are targets of gibberellin signaling in flower development. Proc. Natl. Acad. Sci. USA 101: 7827-7832. Zhang, S., and Wang, X. (2008). Expression pattern of GASA, down-stream genes of DELLA, in Arabidopsis. Chin. Sci. Bull. 53: 3839-3846. Zhang, S.C., Yang, C.W., Peng, J.Z., Sun, S.L., and Wang, X. (2009). GASA5, a regulator of flowering time and stem growth in Arabidopsis thaliana. Plant Mol. Biol. 69: 745-759. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/6126 | - |
dc.description.abstract | 先前利用差異表現法篩選到在綠豆中會受3-5 µm波長紅外光誘導之基因VrGIR1 (gibberellic acid[GA]-and infrared[IR]-induced gene1),其在阿拉伯芥中的相似基因GASA4 (gibberellic acid-stimulated transcripts in Arabidopsis 4)同樣會受吉貝素及紅外光所誘導,因此本研究致力於了解GASA4在荷爾蒙及紅外光訊息傳遞當中所扮演的角色。吉貝素與茉莉酸分別調控植物的生長發育及防禦,但兩種荷爾蒙之間的交互作用目前還不是很明瞭。GASA4目前已知參與在吉貝素的訊息傳遞路徑中,而FAR-RED INSENSITIVE 219 (FIN219)/JAR1在先前的研究發現會參與在遠紅光及茉莉酸訊息傳遞中。本實驗室先前的研究發現GASA4基因會受FIN219的負調控,因此我們對於兩者在茉莉酸與吉貝素訊息傳遞中所扮演的角色,以及彼此之間的調控關係產生高度興趣。在本次研究當中,我們發現gasa4及fin219突變株都呈現對茉莉酸處理較不敏感的外表型,而對吉貝素處理則較野生型敏感。基因表現的研究則發現FIN219能正調控茉莉酸反應的相關基因表現,如MYC2、VSP1及JAZ1,而GASA4則是扮演負調控者的角色。此外我們也發現GASA4能負調控SPINDLY (SPY)基因表現,而SPY在先前的研究已知能影響DELLA的蛋白質活性。最後我們也發現GASA4能負調控吉貝素訊息傳遞中的負調控者DELLA RGA-LIKE3 (RGL3)的表現,而FIN219則正調控RGL3的表現,RGL3已知也會參與茉莉酸訊息傳遞。綜合以上研究結果,我們推測FIN219與GASA4能藉由調控RGL3表現而共同影響植物中茉莉酸及吉貝素訊息傳遞的進行。除了對荷爾蒙的調控外,本研究也發現GASA4會與PSAK及NPQ4共同參與在紅外光抑制下胚軸的訊息傳遞路徑中,GASA4扮演正調控者的角色,而PSAK及NPQ4則為負調控者。已知二氧化碳能吸收4.3 μm紅外光,推測紅外光能藉由促進植物的固碳作用進而影響光合作用效率,本研究發現紅外光照射能增加C4植物PEP carboxylase基因的表現,並且能延緩玉米與阿拉伯芥葉圓體的老化。綜合以上實驗數據,我們認為GASA4能作為連結光與多種荷爾蒙訊息傳遞的重要因子,並且未來能應用於提高作物產量。 | zh_TW |
dc.description.abstract | In previous study, gibberellic acid[GA]-and IR-induced gene1 (VrGIR1) was isolated by differential display from mungbean (Vigina radiate) seedlings irradiated with 3~5 µm IR light. Its homolog in Arabidopsis, GASA4 (gibberellic acid-stimulated transcripts in Arabidopsis) was also induced by GA and IR. My thesis aims to investigate the functions of GASA4 in the crosstalk between GA and IR signaling pathways. Jasmonates (JA) and GA are important plant hormones that mainly mediate defense and growth, respectively. However, direct crosstalk between these two hormone pathways remains largely unknown. GASA4 has been shown to participate in GA signaling, and FAR-RED INSENSITIVE 219 (FIN219)/JAR1 was previously shown to play vital roles in far-red (FR) light and JA signaling pathway. Previous study indicated that GASA4 was down-regulated by FIN219. It is interesting to understand the relationship between FIN219 and GASA4 in the integration of both GA and JA signaling. Here, we analyze the regulation between FIN219 and GASA4 in both GA and JA treatments. The results revealed that gasa4 and fin219 mutant showed an insensitive phenotype to JA treatment, but more sensitive to GA treatment. The gene expression studies indicated that FIN219 positively regulated JA responsive genes, but GASA4 negatively regulated them. GASA4 was also found to regulate a GA responsive gene, SPINDLY (SPY), which can alter DELLA protein activity. Moreover, GASA4 negatively regulated DELLA RGA-LIKE3 (RGL3) expression, one of negative regulator of GA signaling that has been shown to participate in JA signaling, whereas FIN219 positively regulates RGL3 expression. Taken together, our data indicate that FIN219 and GASA4 participate in GA and JA signaling via regulating RGL3 expression. In addition to the regulation of GA and JA signaling, GASA4 was also involved in IR signaling pathway. The IR-induced PSAK and NPQ4 genes were acting in this pathway and regulated by GASA4. CO2 can absorb IR wavelength at about 4.3 µm. IR might affect photosynthetic efficiency by improving CO2 fixation. Our studies revealed that IR promotes the expression of PEP carboxylase gene and delays senescence in leaf discs of maize and Arabidopsis. Taken together, our data indicate that GASA4 functions as a crosstalk between light, including IR, and multiple hormones such as GA and JA, and may serve as a good candidate for future applications in improving crop yields. | en |
dc.description.provenance | Made available in DSpace on 2021-05-16T16:21:29Z (GMT). No. of bitstreams: 1 ntu-102-R00b42002-1.pdf: 2671548 bytes, checksum: f6bfc65c070d903f6c3d8d0d2158d625 (MD5) Previous issue date: 2013 | en |
dc.description.tableofcontents | 中文摘要.......................................................................................................................IV
英文摘要.......................................................................................................................VI 前言 一、緒論..........................................................................................................................1 二、植物的光訊息傳遞..................................................................................................2 三、吉貝素訊息傳遞......................................................................................................3 四、茉莉酸訊息傳遞.....................................................................……………….…….3 五、吉貝素與茉莉酸訊息傳遞之間的交互作用..........................................................5 六、GASA4.....................................................................................................................6 七、GASA家族參與在不同荷爾蒙交互作用的路徑中..............................................6 八、GASA4先前的研究................................................................................................7 九、FIN219.....................................................................................................................8 十、PSAK與NPQ4.......................................................................................................9 十一、PEP carboxylase..................................................................................................10 十二、研究目標............................................................................................................10 材料與方法 一、植物材料與生長條件............................................................................................12 二、質體之建構與基因轉殖........................................................................................12 三、Genomic DNA萃取...............................................................................................13 四、下胚軸測量............................................................................................................13 五、根長測量................................................................................................................13 六、GA促進百分比及MeJA抑制百分比公式.........................................................14 七、花青素之萃取及定量............................................................................................14 八、葉綠素之萃取及定量............................................................................................14 九、種子發芽測試........................................................................................................15 十、RNA萃取與RNA表現量分析...........................................................................15 十一、蛋白質萃取及表現量分析...............................................................................15 CHAPTER I. 阿拉伯芥GASA4與FIN219之間的調控關係在吉貝素與茉莉酸訊息傳遞交互作用中的功能性研究 結果 一、GASA4及FIN219會影響JA及GA相關外表型.............................................17 1-1、荷爾蒙GA及JA對gasa4及fin219突變體下胚軸的影響.............................17 1-2、GASA4與FIN219參與茉莉酸在弱遠紅光下促進花青素累積......................18 1-3、FIN219在各種不同的MeJA濃度下均具有對JA不敏感的根長外表型.......18 1-4、GASA4與FIN219參與吉貝素促進種子萌發..................................................19 二、GASA4與FIN219會彼此調控對方的表現.......................................................19 三、GASA4及FIN219共同調控JA相關基因的表現.............................................20 3-1、JA生合成基因......................................................................................................20 3-2、JA訊息傳遞因子..................................................................................................21 四、GASA4及FIN219共同調控GA相關基因表現................................................21 4-1、DELLA基因.........................................................................................................22 4-2、SPY基因...............................................................................................................22 討論 一、GASA4與FIN219共同參與在吉貝素訊息傳遞路徑........................................23 二、GASA4與FIN219共同參與在茉莉酸訊息傳遞路徑.......................................23 三、GASA4能藉由多條路徑調控GA與JA訊息傳遞的交互作用........................24 四、遠紅光的強弱會造成對GA及JA反應的影響不同..........................................25 五、結論與未來展望……….........................................................................................27 CHAPTER II. 阿拉伯芥GASA4在紅外光訊息傳遞中的功能性研究 結果 一、PSAK大量表現轉殖株在紅外光照射下呈現長下胚軸的外表型.....................29 二、NPQ4大量表現的轉殖株在紅外光照射下同樣呈現長下胚軸的外表型.........29 三、在gasa4突變株背景下的轉殖株會恢復成如同野生型的外表型.....................30 四、紅外光能促進C4植物PEPC基因的表現.........................................................30 五、紅外光能影響葉綠素含量....................................................................................31 討論 一、PSAK、NPQ4及GASA4共同參與紅外光抑制下胚軸延長的路徑中............32 二、紅外光照射能促進植物的光合作用效率.............................................................32 三、紅外光照射能延緩植物葉的老化.........................................................................33 結果圖表.......................................................................................................................34 參考文獻.......................................................................................................................52 附錄一:實驗詳細流程...............................................................................................58 附錄二:使用之引子列表...........................................................................................61 | |
dc.language.iso | zh-TW | |
dc.title | 阿拉伯芥GASA4在光及荷爾蒙交互作用中的功能性研究 | zh_TW |
dc.title | Functional Studies of GASA4 in Light and the Integration of Hormone Signalings in Arabidopsis | en |
dc.type | Thesis | |
dc.date.schoolyear | 101-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 鄭秋萍,張英?,張孟基,趙光裕 | |
dc.subject.keyword | 紅外光,吉貝素,茉莉酸, | zh_TW |
dc.subject.keyword | FIN219,GASA4,Gibberellic acid,Infared light,Jasmonic acid,NPQ4,PSAK,PEP carboxylase,RGL3, | en |
dc.relation.page | 63 | |
dc.rights.note | 同意授權(全球公開) | |
dc.date.accepted | 2013-07-29 | |
dc.contributor.author-college | 生命科學院 | zh_TW |
dc.contributor.author-dept | 植物科學研究所 | zh_TW |
顯示於系所單位: | 植物科學研究所 |
文件中的檔案:
檔案 | 大小 | 格式 | |
---|---|---|---|
ntu-102-1.pdf | 2.61 MB | Adobe PDF | 檢視/開啟 |
系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。