請用此 Handle URI 來引用此文件：
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/61136完整後設資料紀錄
| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 蕭信宏 | |
| dc.contributor.author | Ching-I Chen | en |
| dc.contributor.author | 陳敬宜 | zh_TW |
| dc.date.accessioned | 2021-06-16T10:48:38Z | - |
| dc.date.available | 2018-09-24 | |
| dc.date.copyright | 2013-09-24 | |
| dc.date.issued | 2013 | |
| dc.date.submitted | 2013-08-12 | |
| dc.identifier.citation | Adler, P. N. (2002). Planar signaling and morphogenesis in Drosophila. Dev Cell, 2(5), 525-535.
Attardo, G. M., Hansen, I. A., Shiao, S. H., & Raikhel, A. S. (2006). Identification of two cationic amino acid transporters required for nutritional signaling during mosquito reproduction. J Exp Biol, 209(Pt 16), 3071-3078. Boutros, M., Paricio, N., Strutt, D. I., & Mlodzik, M. (1998). Dishevelled activates JNK and discriminates between JNK pathways in planar polarity and wingless signaling. Cell, 94(1), 109-118. Brown, M. R., Graf, R., Swiderek, K. M., Fendley, D., Stracker, T. H., Champagne, D. E., & Lea, A. O. (1998). Identification of a steroidogenic neurohormone in female mosquitoes. J Biol Chem, 273(7), 3967-3971. Cadigan, K. M., & Nusse, R. (1997). Wnt signaling: a common theme in animal development. Genes Dev, 11(24), 3286-3305. Chen, L. H., & Wilson, M. E. (2012). Dengue and chikungunya in travelers: recent updates. Curr Opin Infect Dis, 25(5), 523-529. Christophers,S.R.(1960). AEDES AEGYPTI THE YELLOW FEVER MOSQUITO:ITS LIFE HISTORY,BIONOMICS AND STRUCTURE. Clevers, Hans. (2006). Wnt/β-Catenin Signaling in Development and Disease. Cell, 127(3). Cox-Singh, J., Davis, T. M., Lee, K. S., Shamsul, S. S., Matusop, A., Ratnam, S., Singh, B. (2008). Plasmodium knowlesi malaria in humans is widely distributed and potentially life threatening. Clin Infect Dis, 46(2), 165-171. Croce, J. C., & McClay, D. R. (2008). Evolution of the Wnt pathways. Methods Mol Biol, 469, 3-18. De, A. (2011). Wnt/Ca2+ signaling pathway: a brief overview. Acta Biochim Biophys Sin (Shanghai), 43(10), 745-756. Fodde, R. (2002). The APC gene in colorectal cancer. Eur J Cancer, 38(7), 867-871. Gonsalves, F. C., & DasGupta, R. (2008). Function of the wingless signaling pathway in Drosophila.MethodsMolBiol, 469, 115-125. Gordon, M. D., Dionne, M. S., Schneider, D. S., & Nusse, R. (2005). WntD is a feedback inhibitor of Dorsal/NF-kappaB in Drosophila development and immunity. Nature, 437(7059), 746-749. Goss, A. M., Tian, Y., Tsukiyama, T., Cohen, E. D., Zhou, D., Lu, M. M., Morrisey, E. E. (2009). Wnt2/2b and beta-catenin signaling are necessary and sufficient to specify lung progenitors in the foregut. Dev Cell, 17(2), 290-298. Gregory, M. A., Phang, T. L., Neviani, P., Alvarez-Calderon, F., Eide, C. A., O'Hare, T., Degregori, J. (2010). Wnt/Ca2+/NFAT signaling maintains survival of Ph+ leukemia cells upon inhibition of Bcr-Abl. Cancer Cell, 18(1), 74-87. Gubler, D. J. (1998). Dengue and dengue hemorrhagic fever. Clin Microbiol Rev, 11(3), 480-496. Gubler, D. J. (2002). The global emergence/resurgence of arboviral diseases as public health problems. Arch Med Res, 33(4), 330-342. Guzman, M. G., Halstead, S. B., Artsob, H., Buchy, P., Farrar, J., Gubler, D. J., Peeling, R. W. (2010). Dengue: a continuing global threat. Nat Rev Microbiol, 8(12 Suppl), S7-16. Hagedorn, H. H., O'Connor, J. D., Fuchs, M. S., Sage, B., Schlaeger, D. A., & Bohm, M. K. (1975). The ovary as a source of alpha-ecdysone in an adult mosquito. Proc Natl Acad Sci U S A, 72(8), 3255-3259. Hansen, I. A., Attardo, G. M., Park, J. H., Peng, Q., & Raikhel, A. S. (2004). Target of rapamycin-mediated amino acid signaling in mosquito anautogeny. Proc Natl Acad Sci U S A, 101(29), 10626-10631. Harker, B. W., Hong, Y. S., Sim, C., Dana, A. N., Bruggner, R. V., Lobo, N. F., Collins, F. H. (2012). Transcription profiling associated with life cycle of Anopheles gambiae. J Med Entomol, 49(2), 316-325. Kamareddine, L. (2012). The biological control of the malaria vector. Toxins (Basel), 4(9), 748-767. Kozopas, K. M., & Nusse, R. (2002). Direct flight muscles in Drosophila develop from cells with characteristics of founders and depend on DWnt-2 for their correct patterning. Dev Biol, 243(2), 312-325. Kuhl, M., Sheldahl, L. C., Park, M., Miller, J. R., & Moon, R. T. (2000). The Wnt/Ca2+ pathway: a new vertebrate Wnt signaling pathway takes shape. Trends Genet, 16(7), 279-283. Kuwata, R., Nga, P. T., Yen, N. T., Hoshino, K., Isawa, H., Higa, Y., Takagi, M. (2013). Surveillance of Japanese Encephalitis Virus Infection in Mosquitoes in Vietnam from 2006 to 2008. Am J Trop Med Hyg. Lea, A. O. (1967). The medial neurosecretory cells and egg maturation in mosquitoes. J Insect Physiol, 13(3), 419-429. McKay, D. J., Estella, C., & Mann, R. S. (2009). The origins of the Drosophila leg revealed by the cis-regulatory architecture of the Distalless gene. Development, 136(1), 61-71. Nash, D., Mostashari, F., Fine, A., Miller, J., O'Leary, D., Murray, K., Layton, M. (2001). The outbreak of West Nile virus infection in the New York City area in 1999. N Engl J Med, 344(24), 1807-1814. Niehrs, C. (2012). The complex world of WNT receptor signalling. Nat Rev Mol Cell Biol, 13(12), 767-779. Nolo, R., Abbott, L. A., & Bellen, H. J. (2000). Senseless, a Zn finger transcription factor, is necessary and sufficient for sensory organ development in Drosophila. Cell, 102(3), 349-362. Noselli, S., & Agnes, F. (1999). Roles of the JNK signaling pathway in Drosophila morphogenesis. Curr Opin Genet Dev, 9(4), 466-472. Nusse, R., & Varmus, H. E. (1982). Many tumors induced by the mouse mammary tumor virus contain a provirus integrated in the same region of the host genome. Cell, 31(1), 99-109. Peifer, M., & Polakis, P. (2000). Wnt signaling in oncogenesis and embryogenesis--a look outside the nucleus. Science, 287(5458), 1606-1609. Rijsewijk, F., Schuermann, M., Wagenaar, E., Parren, P., Weigel, D., & Nusse, R. (1987). The Drosophila homolog of the mouse mammary oncogene int-1 is identical to the segment polarity gene wingless. Cell, 50(4), 649-657. Rulifson, E. J., Wu, C. H., & Nusse, R. (2000). Pathway specificity by the bifunctional receptor frizzled is determined by affinity for wingless. Mol Cell, 6(1), 117-126. Sato, M., Umetsu, D., Murakami, S., Yasugi, T., & Tabata, T. (2006). DWnt4 regulates the dorsoventral specificity of retinal projections in the Drosophila melanogaster visual system. Nat Neurosci, 9(1), 67-75. Sharma, R. P., & Chopra, V. L. (1976). Effect of the Wingless (wg1) mutation on wing and haltere development in Drosophila melanogaster. Dev Biol, 48(2), 461-465. Siegfried, E., & Perrimon, N. (1994). Drosophila wingless: a paradigm for the function and mechanism of Wnt signaling. Bioessays, 16(6), 395-404. Strutt, D., Madder, D., Chaudhary, V., & Artymiuk, P. J. (2012). Structure-function dissection of the frizzled receptor in Drosophila melanogaster suggests different mechanisms of action in planar polarity and canonical Wnt signaling. Genetics, 192(4), 1295-1313. Suthar, M. S., Diamond, M. S., & Gale, M., Jr. (2013). West Nile virus infection and immunity. Nat Rev Microbiol, 11(2), 115-128. Tolle, M. A. (2009). Mosquito-borne diseases. Curr Probl Pediatr Adolesc Health Care, 39(4), 97-140. Trampuz, A. (2003). Clinical review: Severe malaria. Crit Care, 7(4), 315-323. van Ooyen, A., & Nusse, R. (1984). Structure and nucleotide sequence of the putative mammary oncogene int-1; proviral insertions leave the protein-encoding domain intact. Cell, 39(1), 233-240. Veeman, M. T., Axelrod, J. D., & Moon, R. T. (2003). A second canon. Functions and mechanisms of beta-catenin-independent Wnt signaling. Dev Cell, 5(3), 367-377. Weston, C. R., & Davis, R. J. (2002). The JNK signal transduction pathway. Curr Opin Genet Dev, 12(1), 14-21. Wilder, E. L., & Perrimon, N. (1995). Dual functions of wingless in the Drosophila leg imaginal disc. Development, 121(2), 477-488. Yoshikawa, S., McKinnon, R. D., Kokel, M., & Thomas, J. B. (2003). Wnt-mediated axon guidance via the Drosophila Derailed receptor. Nature, 422(6932), 583-588. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/61136 | - |
| dc.description.abstract | 病媒蚊傳播的寄生蟲或病毒性疾病，如瘧疾、登革熱、黃熱病…等，每年皆造成上億的病例，超過百萬人死亡。但目前針對這些傳染性疾病大多缺乏有效的疫苗，此外，有些流行地區的瘧原蟲已對抗瘧疾藥物產生抗藥性；因此，許多研究團隊開始思考藉由控制病媒蚊的傳病能力，以達到控制疾病的目的。其中，藉由生物防治的方式來達到降低病媒蚊的族群數量，被認為是一個可行的替代性策略。過去的研究已證實Wnt signaling pathway參與果蠅的胚胎發育與細胞極性調控，但在埃及斑蚊中對Wnt signaling pathway的研究卻付之闕如，因此我們亟欲探討Wnt signaling pathway於埃及斑蚊中是否在生長發育上也扮演重要角色。本實驗室過去利用RNAi的方式，分別抑制埃及斑蚊中Frizzle2 (Wnt的受體)與Wnt740 (Fz的配體)的表現，發現吸血後的母蚊相較於對照組，其產下的卵數大幅減少。此外我們也發現，在蛹期抑制AaWnt740的表現時，其羽化的比例也明顯降低。並且在我們的實驗當中，觀察到母蚊在羽化過程中無法完全羽化(Incomplete eclosion)。我們更進一步證明，此種羽化不完全的現象是由非典型的Wnt signaling pathway，即planar cell polarity (PCP) pathway所調控。本研究結果將有助於未來發展新的病媒控制策略，以控制病媒傳染病之傳播。 | zh_TW |
| dc.description.abstract | Mosquito-borne diseases are the most devastating agents for human beings, such as malaria, dengue fever, West Nile fever…etc. The WHO reported that some 2.5 billion people are now at risk and more than a million people are killed by mosquito-borne diseases annually. Up to now, no effective vaccine or drug has been developed for most of these diseases. Therefore, any possible avenue for developing novel control strategies against mosquito-borne diseases is urgently needed. Wnt signaling pathway is shown to be participated in the embryonic development and cell polarity in Drosophila. However, the functions of Wnt signaling remain largely unknown in the mosquito. In this study, we attempt to characterize the role of Wnt signaling in the yellow fever mosquito, Aedes aegypti. Our previous results showed that silencing of Frizzl2 (the Wnt signaling pathway receptor) and Wnt (the Wnt signaling pathway ligand) resulted in the reduction of eggs production. Interestingly, silencing of Wnt reduced the eclosion rate in the mosquito. Therefore, we would like to elucidate the role of Wnt in the regulation of mosquito morphogenesis, which will be crucial for the development of novel vector control strategy. Our results showed that Wnt was highly expressed in the mosquito pupal stage in terms of transcriptional level. Intriguingly, we observed that silencing of Wnt740 exhibited an incomplete eclosion phenotype in the mosquito pupae. Furthermore, we demonstrated that this phenotype was regulated by planar cell polarity (PCP) pathway, the non-canonical Wnt signaling pathway. Information gathered in this study will pave the way toward the establishment of efficient strategies for vector control by using molecular engineering approaches. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-16T10:48:38Z (GMT). No. of bitstreams: 1 ntu-102-R00445202-1.pdf: 2306488 bytes, checksum: 6b7977dd7f0badffc8a7190328b7ccef (MD5) Previous issue date: 2013 | en |
| dc.description.tableofcontents | 誌謝 i
中文摘要 ii Abstract iii 目錄 iv 圖目錄 vi 第一章、緒論 1 1.1. 病媒蚊傳播疾病 (Mosquito-borne diseases) 1 1.1.1. 登革熱 2 1.1.2. 瘧疾 3 1.2. 蚊子的生活史 (Mosquito life cycle) 4 1.3. 蚊子的卵黃生成機制 (Mosquito vitellogenesis) 5 1.4. Wnt signaling pathway 6 1.5. 實驗動機與假說 8 第二章、材料與方法 9 2.1. 實驗步驟與流程 9 2.1.1. 埃及斑蚊的飼養 9 2.1.2. 餵血 9 2.1.3. 演化樹分析 10 2.1.4. 胺基酸序列比對 10 2.1.5. RNA萃取 10 2.1.6. 反轉錄(Reverse Transcription；RT) 11 2.1.7. 聚合酶鏈鎖反應(Polymerase chain reaction；PCR) 11 2.1.8. RNA干擾作用(RNA interference；RNAi) 11 2.1.8.1. 質體建構(Plasmid construct) 11 2.1.8.2. 雙股RNA製備(Double stranded RNA；dsRNA) 12 2.1.9. 抑制成效的確認 (Knockdown efficiency) 13 2.1.10. 產卵率實驗 14 2.1.11. 羽化率實驗 14 2.1.12. 羽化不完全現象與比例 15 2.1.13. 西方點墨法 (Western blot) 15 2.2. 使用之試劑及配置方式 17 第三章、結果 20 3.1. Wnt family genes 之演化樹(phylogenetic tree)分析 20 3.2. 埃及斑蚊Wnts的胺基酸序列比對 20 3.3. 埃及斑蚊Wnts於不同發育時期之 mRNA表現 21 3.4. 抑制埃及斑蚊 Wnt740時羽化不完全現象 21 3.5. 抑制埃及斑蚊 Wnt740時羽化不完全母蚊之比例 22 3.6. 埃及斑蚊MAP、Tensin、Sens、DLL於不同發育時期之 mRNA表現及其受Wnt740之影響 22 3.7. 羽化不完全現象與Canonical Wnt pathway之調控關係 23 3.8. 羽化不完全現象與 Wnt/Calcium (Ca2+) pathway之調控關係 23 3.9. 羽化不完全現象與planar cell polarity（PCP）pathway之調控關係 24 3.10. 抑制埃及斑蚊JNK誘導羽化不完全現象 24 第四章、討論 26 圖表(Figures) 30 附錄 49 參考文獻 51 | |
| dc.language.iso | zh-TW | |
| dc.title | Wnt參與埃及斑蚊變態過程之探討 | zh_TW |
| dc.title | Wnt is essential for the morphogenesis in the mosquito Aedes aegypti | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 101-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 詹智強,吳君泰 | |
| dc.subject.keyword | 埃及斑蚊,Wnt740,Wnt signaling pathway,JNK,羽化不完全, | zh_TW |
| dc.subject.keyword | Aedes aegypti,Wnt740,Wnt signaling pathway,JNK,Incomplete eclosion, | en |
| dc.relation.page | 55 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2013-08-12 | |
| dc.contributor.author-college | 醫學院 | zh_TW |
| dc.contributor.author-dept | 微生物學研究所 | zh_TW |
| 顯示於系所單位： | 微生物學科所 | |
文件中的檔案：
| 檔案 | 大小 | 格式 | |
|---|---|---|---|
| ntu-102-1.pdf 目前未授權公開取用 | 2.25 MB | Adobe PDF |
系統中的文件，除了特別指名其著作權條款之外，均受到著作權保護，並且保留所有的權利。