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http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/61091完整後設資料紀錄
| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 蔡偉博(Wei-Bor Tsai) | |
| dc.contributor.author | Ming-Chun Keng | en |
| dc.contributor.author | 耿明雋 | zh_TW |
| dc.date.accessioned | 2021-06-16T10:45:56Z | - |
| dc.date.available | 2018-08-17 | |
| dc.date.copyright | 2013-08-17 | |
| dc.date.issued | 2013 | |
| dc.date.submitted | 2013-08-12 | |
| dc.identifier.citation | [1] Ratner BD, Bryant SJ. Biomaterials: Where we have been and where we are going. Annual Review of Biomedical Engineering. 2004;6:41-75.
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/61091 | - |
| dc.description.abstract | Nonfouling表面，由於能夠抵抗非專一性蛋白質的吸附，以及更進一步可抵抗細胞貼附，已經被廣泛使用在生醫材料和生物晶片上。在本研究第一部分首先將表面藉由化學氣相層積(Chemical Vapor Deposition ,CVD)將表面改質，利用合成方式製造出具有雙離子性的sulfobetaine & carboxybetaine的化合物。並利用其官能基在表面進行反應，使其能達到表面性質改變而達到抗生物性貼附。本研究主要探討藉由所合成之兩個單體與表面反應時的濃度差距，搭配利用石英微量天平測量其吸附量的變化與血清蛋白吸附和纖維母細胞(L929)貼附定量計算等方式來進行表面分析。
另外第二部分利用CVD 的改質表面，藉由Photo-cross-linking接上不同種類的聚合物，例如PEG，Poly NIPAAm等，讓表面上產生親/疏水性質，更進一步在表面同時接上兩種不同性質化合物，製造出細胞圖樣。 | zh_TW |
| dc.description.abstract | Nonfouling surface has been widely used in biomaterials and biosensors due to its potential resistance in non-specific adsorption and cell adhesion. In the first part of this study, first we modified substrate surface by the chemical vapor deposition (CVD) technology. Second , we synthesized the zwitterion sulfobetaine & carboxybetaine monomers, using the corresponding functional group grafting to the CVD coated surface change the character of surface to resisting bio adhesion. In this study, we investigated the surface influence between the concentration of zwitterion monomers by the using of XPS, the protein adsorption by QCM measurement, and the adhesion of fibroblast L929 cell.
In the second part of this study, we modified the CVD coated surface by the photo-cross-linking. By the using of photochemical conjugation, we make polymers conjugated to CVD surfaces. By grafting different polymer, such as poly(ethylene glycol) PEG and poly(N-isopropylacrylamide) (NIPAAm) , change the property of surface. Furthermore, we grafted different polymers on the same surface, producing the cell patterning. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-16T10:45:56Z (GMT). No. of bitstreams: 1 ntu-102-R00524065-1.pdf: 3506185 bytes, checksum: 9047b7003b7b3fa8fd686605a76f7cad (MD5) Previous issue date: 2013 | en |
| dc.description.tableofcontents | Content
致謝 I 摘要 III Abstract V Content VII List of figures VII List of tables XV Chapter 1 Introduction 1 1.1 Nonfouling surface 3 1.2 Nonfouling materials 5 1.2.1 PEG (poly(ethylene glycol)) 5 1.2.2 Zwitterionic polymers 6 1.2.2.1 Phosphorylcholine (PC) 6 1.2.2.2 Sulfobetaine (SB) 13 1.2.2.3 Carboxybetaine (CB) 18 1.2.2.4 Characteristic of Zwitterionic polymers 19 1.2.2.5 Interaction of protein and zwitterions-modified surface 24 1.3 Surface modification by nonfouling materials 25 1.3.1 Self-assembled monolyer (SAM) 25 1.3.2 Graft polymerization 26 1.3.3 Hydrophobic interaction 27 1.3.4 Grafting to method 28 1.3.5 Layer-by-layer deposition 28 1.3.6 Catechol adhesion 30 1.3.7 Chemical Vapor Deposition (CVD) 33 1.4 Cell patterning 36 1.5 Research motivation 38 1.6 Research objective 40 Chapter 2 Materials and Methods 41 2.1 Chemicals 41 2.1.1 Chemicals for synthesizing 3-((3-azidopropyl)dimethylammonio)propane-1-sulfonate (SBAZ) 41 2.1.2 Chemicals for synthesizing (3-((3-(4-formylphenoxy)propyl)dimethylammonio)propanoate) (CBBA) 42 2.1.3 Polymers 42 2.1.4 Cell culture 42 2.2 Experimental instrument 43 2.3 Experimental materials 44 2.4 Solution formula 45 2.5 Methods 47 2.5.1 Synthesis of the 3-((3-azidopropyl)dimethylammonio) propane-1-sulfonate (SBAZ) 47 2.5.2 Synthesis of the 3-((3-(4-formylphenoxy)propyl) dimethylammonio)propanoate (CBBA) 48 2.5.3 Surface modification by click chemistry 49 2.5.4 Schiff base chemistry between CBBA and amine-surface 51 2.5.5 Photochemical conjugation of polymers to benzoyl surfaces 54 2.5.6 The procedure of cell patterning 56 2.5.7 XPS Analysis 57 2.5.8 Water Contact Angle (WCA) 58 2.5.9 QCM measurement 59 2.5.10 Cell culture 61 2.5.11 Statistical analysis 62 Chapter 3 The property and characterization of zwitterionic monomer 3-((3-azidopropyl)dimethylammonio)propane-1-sulfonate (SBAZ) 71 3.1 The NMR and MASS of SBAZ 71 3.2 Surface characterization 72 3.3 The amount of protein adsorption. 73 3.4 Cell adhesion experiment 74 3.5 Discussion 75 Chapter 4 The property and characterization of zwitterionic monomer (3-((3-(4-formylphenoxy)propyl) dimethylammonio)propanoate ) CBBA 87 4.1 The NMR and MASS of of CBBA 87 4.2 Surface characterization 88 4.3 The amount of protein adsorption. 89 4.4 Cell adhesion experiment 90 4.5 Discussion 91 Chapter 5 The cell patterning from multiple functional surface. 103 5.1 The nonfouling property of PEG grafting surface 103 5.2 The cell sheet property of Poly NIPAAm grafting surface 104 5.3 Cell patterning 104 5.4 Discussion 106 Chapter 6 Conclusions 113 Reference 115 | |
| dc.language.iso | en | |
| dc.subject | 細胞貼附 | zh_TW |
| dc.subject | 蛋白質吸附 | zh_TW |
| dc.subject | 生物惰性 | zh_TW |
| dc.subject | sulfobetaine | zh_TW |
| dc.subject | cell patterning | en |
| dc.subject | sulfobetaine methacrylate | en |
| dc.subject | cell adhesion | en |
| dc.subject | protein adsorption | en |
| dc.subject | photochemistry | en |
| dc.subject | nonfouling | en |
| dc.title | 兩性離子分子修飾於功能性化學氣相沈積表面
以抗細胞貼附之研究 | zh_TW |
| dc.title | Study of zwitterionic molecule-modified functional chemical vapor deposition surfaces for anti-cell adhesion | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 101-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 陳賢燁(Hsien-Yeh Chen),黃聲東(Sheng-Tung Huang) | |
| dc.subject.keyword | 生物惰性,sulfobetaine,細胞貼附,蛋白質吸附, | zh_TW |
| dc.subject.keyword | nonfouling,sulfobetaine methacrylate,cell adhesion,protein adsorption,photochemistry,cell patterning, | en |
| dc.relation.page | 128 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2013-08-13 | |
| dc.contributor.author-college | 工學院 | zh_TW |
| dc.contributor.author-dept | 化學工程學研究所 | zh_TW |
| 顯示於系所單位： | 化學工程學系 | |
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