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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 葉開溫(Kai-Wun Yeh) | |
| dc.contributor.author | Shun-Te Wang | en |
| dc.contributor.author | 王順德 | zh_TW |
| dc.date.accessioned | 2021-06-16T10:39:25Z | - |
| dc.date.available | 2017-08-16 | |
| dc.date.copyright | 2013-08-16 | |
| dc.date.issued | 2013 | |
| dc.date.submitted | 2013-08-13 | |
| dc.identifier.citation | 吳佩穎 (2012)。維他命C與一氧化氮於文心蘭及阿拉伯芥開花過程之協同作用。國立台灣大學植物科學研究所碩士論文。
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/60976 | - |
| dc.description.abstract | 本研究以南西品系文心蘭作為材料，探討的鈉氫離子反向運輸蛋白 (NHX, Na+/H+ exchanger) 是否參與了文心蘭對抗滲透壓逆境的分子機制。NHX為植物細胞中重要的離子運輸蛋白。可以交換等量正一價的陽離子以及氫離子穿過細胞的膜狀構造。其位置可能在液胞膜、高基氏體或是細胞膜上，並參與細胞內pH值調節、鉀離子及鈉離子的平衡，因此與植物對滲透壓逆境的耐受性有關。目前NHX已經被成功轉殖至多種作物，並改善植物對於鹽鹼環境的耐受性。文心蘭則為台灣重要的切花外銷作物，在自然環境中為附生性植物 (epiphyte)，對於滲透壓逆境有著相當高的耐受性。
我們首先從南西文心蘭的基因轉錄體資料庫中 (transcriptome database)中釣取兩條NHX核酸序列並命名為OgNHX1以及OgNHX6。藉由RT-PCR以及realtime-PCR檢測，發現這兩個基因在文心蘭的各個部位皆有相近的表現量。OgNHX1和OgNHX6的表現量不會受到外加鹽逆境或是過氧化氫的誘導，但在幼年時期的組織表現量較高。接著我們釣取這兩個基因的啟動子，並掛載報導基因轉入阿拉伯芥。發現轉植株的GUS活性在幼年阿拉伯芥活性較強，且會受到外加鹽逆境的誘導，最後我們也將OgNHX1以及OgNHX6轉入阿拉伯芥大量表現。雖然轉植株的根長和側根數有些微的減少，但外表型和耐鹽性皆與對照組無顯著差異。因此推測OgNHX1和OgNHX6的生理功能和過去報導能夠對抗鹽逆境的NHXs有所不同 | zh_TW |
| dc.description.abstract | Oncidium Gower Ramsey is one of the most important horticulture export product in Taiwan. Most of species from Orchidaceae family, including Oncidium Gower Ramsey, are originated in tropical areas Thus they have several special structures corresponding to drought environments.
Plant constantly challenged by external abiotic stress including drought, cold and salinity. In order to adapt to osmotic unbalance resulted from these abiotic stresses, plant evolved several special mechanisms to adjust its internal ion distribution such as NHXs cation/H+ antiporters. In previous studies, NHXs had considered as important regulators in respond to salinity stress. We identified two putative NHX genes from Oncidium Gower Ramsey named “OgNHX1” and “OgNHX6”. These two genes expressed equally in different parts of Oncidium tissues, and surprisedly, unchanged under external salt or hydrogen peroxide treatment. The phenotypes of transgenic Arabidopsis overexpressing OgNHX1/6 showed no differentces comparing to wild-type plants when grown in salnity environment. However the results from stage-dependent realtime-PCR and promoter-GUS analysis suggest possible connections between OgNHXs and plant development. Our results suggest the functions of OgNHX1 and OgNHX6 are atypical to pervious studies, in which consider NHXs as efficient candicates to generate salt-tolerant plants. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-16T10:39:25Z (GMT). No. of bitstreams: 1 ntu-102-R00b42006-1.pdf: 2828813 bytes, checksum: 1fcd0b34e26e0bdda8bf5498eee43074 (MD5) Previous issue date: 2013 | en |
| dc.description.tableofcontents | 致謝 ii
目錄 iv 圖表目錄 vi 附錄目錄 vii 中文摘要 viii 英文摘要 ix 第一章 前言 1 第一節 文心蘭概述 1 第二節 植物NHX antiporter的介紹 2 第三節 鹽度對植物的影響 6 第四節 本論文研究目的 14 第二章 材料與方法 15 第一節：實驗材料 15 第二節：基因表現量測定 15 第三節：載體構築及其他基本分生技術 19 第四節：基因全長釣取及啟動子釣取 25 第五節：阿拉伯芥種植、轉殖及處理 35 第六節：GUS報導基因的染色及測定 37 第三章 結果 40 第一節：文心蘭NHX基因的釣取與序列分析 40 第二節：文心蘭內生性OgNHX1和OgNHX6表現量檢定 41 第三節：文心蘭內生性OgNHX1和OgNHX6在鹽處理下的表現量檢定 42 第四節：文心蘭內生性OgNHX1和OgNHX6在過氧化氫處理下的表現量檢定 42 第五節：阿拉伯芥OgNHX1和OgNHX6轉殖株的建立與觀察 43 第六節：阿拉伯芥OgNHX1和OgNHX6轉殖株在高鹽環境的生長情形 44 第七節：啟動子釣取與序列分析 45 第八節：阿拉伯芥pOgNHX1::GUS和pOgNHX6::GUS轉殖株建立與染色觀察 45 第九節：阿拉伯芥pOgNHX1::GUS和pOgNHX6::GUS轉殖株受鹽逆境的誘導 46 第四章 討論 48 第一節：OgNHXs的親緣性分析 48 第二節：OgNHX1與鹽逆境的關係 49 第三節：文心蘭OgNHX1的啟動子序列與調控 51 第四節：OgNHX1與植物生長發育的關係 52 第五節：文心蘭NHX6的啟動子序列與調控 54 第六節：OgNHX6在文心蘭中所扮演的角色 55 未來展望 57 參考文獻 58 圖表 68 附錄 86 | |
| dc.language.iso | zh-TW | |
| dc.title | 南西文心蘭鈉氫離子反向運輸蛋白OgNHX1及OgNHX6之功能性探討 | zh_TW |
| dc.title | Functional study of Na+/H+ exchangers OgNHX1 and OgNHX6 from Oncidium Gower Ramsey | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 101-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 王淑珍(Shu-Jen Wang),葉信宏(Hsin-Hung Yeh),陳仁治(Jen-Chih Chen),張英?(Ing-Feng Chang) | |
| dc.subject.keyword | NHX 離子反向運輸蛋白,南西文心蘭,耐鹽,非生物逆境,離子平衡, | zh_TW |
| dc.subject.keyword | NHX antiporters,Oncidium Gower Ramsey,Salt tolerance,Abiotic stresses,ion equilibrium, | en |
| dc.relation.page | 94 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2013-08-13 | |
| dc.contributor.author-college | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 植物科學研究所 | zh_TW |
| 顯示於系所單位： | 植物科學研究所 | |
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