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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 謝學真(Hsyue-Jen Hsieh) | |
dc.contributor.author | Kai-Ping Chen | en |
dc.contributor.author | 陳凱評 | zh_TW |
dc.date.accessioned | 2021-06-16T10:32:14Z | - |
dc.date.available | 2023-12-31 | |
dc.date.copyright | 2013-08-27 | |
dc.date.issued | 2013 | |
dc.date.submitted | 2013-08-14 | |
dc.identifier.citation | [1] 周奕宏, 以電紡絲法製備幾丁聚醣、硫酸軟骨素和聚乙烯醇複合奈米纖維之研究. 國立臺灣大學化學工程研究所碩士論文, 2011.
[2] Fuchs, J.R., Nasseri, B.A. and Vacanti, J.P., Tissue engineering: a 21st century solution to surgical reconstruction. Ann Thorac Surg, 2001, 72: 577-591. [3] Langer, R. and Vacanti, J.P., Tissue Engineering. Science, 1993, 260: 920-926. [4] Bowers, S.L.K., Banerjee, I. and Baudino, T.A., The extracellular matrix: At the center of it all. Journal of Molecular and Cellular Cardiology, 2010, 48: 474-482. [5] Yim, E.F.K. and Leong, K.W., Significance of synthetic nanostructures in dictating cellular response. Nanomedicine: Nanotechnology, Biology and Medicine, 2005, 1: 10-21. [6] Clyne, A.M., Thermal Processing of Tissue Engineering Scaffolds. Journal of Heat Transfer-Transactions of the ASME, 2011, 133: 1-8. [7] Curtis, A.S. and Wilkinson, C. D., Reactions of cells to topography. Journal of Biomaterials Science, Polymer Edition, 1998, 9: 1313-1329. [8] Lankalapalli, S. and Kolapalli, V.R.M., Polyelectrolyte complexes: A review of their applicability in drug delivery technology. Indian Journal of Pharmaceutical Sciences, 2009, 71: 481-487 [9] Denuziere, A., Ferrier, D., Damour, O. and Domard, A., Chitosan-chondroitin sulphate and chitosan-hyaluronate polyelectrolyte complexes: biological properties. Biomaterials, 1998, 19: 1275–1285. [10] Oyarzun-Ampuero, F.A., Brea, J., Loza, M.I., Torres, D. and Alonso, M.J. Chitosan-hyaluronic acid nanoparticles loaded with heparin for the treatment of asthma. Int. J. Pharm, 2009, 381: 122–129. [11] Teo, W.E. and Ramakrishna, S., A review on electrospinning design and nanofibre assemblies. Nanotechnology, 2006, 17: R89-R106. [12] Taylor, G., Disintegration of Water Drops in an Electric Field. Proceedings of the Royal Society of London. Series A. Mathematical and Physical Sciences, 1964, 280: 383-397. [13] Taylor, G., The Coalescence of Closely Spaced Drops when they are at Different Electric Potentials. Proceedings of the Royal Society of London. Series A. Mathematical and Physical Sciences, 1968, 306: 423-434. [14] Rayleigh, L., On the equilibrium of liquid conducting masses charged with electricity. Philosophical Magazine, 1882, 14: 184-186. [15] Han, T., Yarin, A.L and Reneker, D.H., Viscoelastic electrospun jets: Initial stresses and elongational rheometry. Polymer, 2008, 49: 1651-1658. [16] Yarin, A., Koombhongse, S., and Reneker, D., Taylor cone and jetting from liquid droplets in electrospinning of nanofibers. Journal of Applied Physics, 2001, 90: 4836-4846. [17] Shenoy, S.L., Bates, W.D., Frisch, H.L. and Wnek, G.E., Role of chain entanglements on fiber formation during electrospinning of polymer solutions: good solvent, non-specific polymer-polymer interaction limit. Polymer, 2005, 46: 3372-3384. [18] Matsumoto, H., Yako, H., Minagawa, M. and Tanioka, A., Characterization of chitosan nanofiber fabric by electrospray deposition: Electrokinetic and adsorption behavior. Journal of Colloid and Interface Science, 2007, 310: 678-681. [19] Doshi, J. and Reneker, D.H., Electrospinning process and applications of electrospun fibers. Journal of Electrostatics, 1995, 35: 151-160. [20] Liu, H., and Hsieh, Y.L., Ultrafine fibrous cellulose membranes from electrospinning of cellulose acetate. Journal of Polymer Science Part B: Polymer Physics, 2002, 40: 2119-2129. [21] Sun, B., Long, Y.Z., Zhang H.D., Li, M.M., Duvail J.L., Jiang, X.Y. and Yin, H.L., Advances in three-dimensional nanofibrous macrostructures via electrospinning. Progress in Polymer Science, 2013. [22] Kim, S.J., Jang, D.H., Park, W.H. and Min, B.M., Fabrication and characterization of 3-dimensional PLGA nanofiber/microfiber composite scaffolds. Polymer , 2010, 51:1320-1327. [23] Yan, G., Yu, J., Qiu, Y., Yi, X., Lu, J., Zhou, X. and Bai, X., Self-assembly of electrospun polymer nanofibers: a general phenomenon generating honeycomb-patterned nanofibrous structures. Langmuir, 2011, 27: 4285–4289. [24] Thandavamoorthy, S., Gopinath, N. and Ramkumar, S.S., Self-assembled honeycomb polyurethane nanofibers. Journal of Applied Polymer Science, 2006, 101: 3121–3124. [25] Huang, Z.M., Zhang Y.Z., Kotaki, M. and Ramakrishna, S., A review on polymer nanofibers by electrospinning and their applications in nanocomposites. Composites Science and Technology, 2003, 63: 2223-2253. [26] Ohkawa, K., Cha, D.I., Kim, H., Nishida, A. and Yamamoto, H., Electrospinning of chitosan. Macromolecular Rapid Communications, 2004, 25: 1600-1605. [27] Lee, K.Y., Jeong, L., Kang, Y.O., Lee S.J. and Park, W.H., Electrospinning of polysaccharides for regenerative medicine. Advanced Drug Delivery Reviews, 2009, 61: 1020-1032. [28] Wang, X., Um I.C., Fang, D., Okamoto, A., Hsiao B.S., and Chu, B., Formation of water-resistant hyaluronic acid nanofibers by blowing-assisted electro-spinning and non-toxic post treatments. Polymer, 2005, 46: 4853-4867. [29] Liu, Y., Ma, G., Fang, D., Xu, J., Zhang H. and Nie, J., Effects of solution properties and electric field on the electrospinning of hyaluronic acid. Carbohydrate Polymers, 2011, 89: 1011-1015. [30] Brenner, E.K., Schiffman, J.D., Thompson, E.A., Toth, L.J., and Schauer, C.L., Electrospinning of hyaluronic acid nanofibers from aqueous ammonium solutions. Carbohydrate Polymers, 2012, 87: 926-929. [31] Ji, Y., Ghosh, K., Shu, X.Z., Li, B., Sokolov, J.C., Prestwich, G.D., Clark, R.A., Rafailovich, M.H., Electrospun three-dimensional hyaluronic acid nanofibrous scaffolds. Biomaterials, 2006, 27:3782-3792. [32] Ma, G., Liu, Y., Fang, D., Chen, J., Peng, C., Fei, X., and Nie, J., Hyaluronic acid/chitosan polyelectrolyte complexes nanofibers prepared by electrospinning. Materials Letters, 2012, 74: 78-80. [33] Hsieh, H.J., Chen, P.H., Kuo, T.Y., Liu, F.H., Hwang, Y.H., Ho, M.H., Wang, D.M. and Lai, J.Y., Use of dicarboxylic acids to improve and diversify the material properties of porous chitosan membranes. Journal of Agricultural and Food Chemistry, 2008, 56: 9015-9021. [34] Dumitriu, S., Polymeric Biomaterials, 2nd ed. Dekker: New York, 2002. [35] Ilium, L., Chitosan and Its Use as a Pharmaceutical Excipient. Pharmaceutical Research, 1998, 15: 1326-1331. [36] Muzzarelli, R., Baldassarre, V., Conti, F., Ferrara, P., Biagini, G., Gazzanelli, G. and Vasi, V., Biological activity of chitosan: ultrastructural study. Biomaterials, 1988, 9: 247-252. [37] Shirakura, M., Tanimoto, K., Eguchi, H., Miyauchi, M., Nakamura, H., Hiyama, K., Tanaka, E., Takata, T. and Tanne, K., Activation of the hypoxia-inducible factor-1 in overloaded temporomandibular joint, and induction of osteoclastogenesis. Biochem Biophys Res Commun, 2010, 393: 800-805. [38] Klokkevold, P.R., Subar, P., Fukayama H. and Bertolami, C.N., Effect of chitosan on lingual hemostasis in rabbits with platelet dysfunction induced by epoprostenol. Journal of Oral and Maxillofacial Surgery, 1992, 50: 41-45. [39] Muzzarelli, R.A., Zucchini, C., Ilari, P., Pugnaloni, A., Mattioli Belmonte, M., Biagini, G. and Castaldini, C., Osteoconductive properties of methylpyrrolidinone chitosan in an animal model. Biomaterials, 1993, 14: 925-929. [40] No, H.K. and Meyers, S.P., Crawfish Chitosan as a Coagulant in Recovery of Organic Compounds from Seafood Processing Streams. Journal of Agricultural and Food Chemistry, 1989, 37: 580-583. [41] Senstad, C. and Mattiasson, B., Affinity-precipitation using chitosan as ligand carrier. Biotechnology and Bioengineering, 1989,33: 216-220. [42] Wisuitiprot, W., Somsiri, A., Ingkaninan, K. and Waranuch, N., In vitro human skin permeation and cutaneous metabolism of catechins from green tea extract and green tea extract-loaded chitosan microparticles. International Journal of Cosmetic Science, 2011, 33:572-579. [43] Sugano, M., Fujikawa, T., Hiratsuji, Y., Nakashima, K., Fukuda, N., and Hasegawa, Y., A Novel Use of Chitosan as a Hypocholesterolemic Agent in Rats. American Journal of Clinical Nutrition, 1980, 33: 787-793. [44] Fakhari, A. and Berkland, C., Applications and emerging trends of hyaluronic acid in tissue engineering, as a dermal filler and in osteoarthritis treatment. Acta Biomaterialia, 2013, 9: 7081-7092. [45] Laurent, T. and Fraser, J., Hyaluronan. FASEB J, 1992, 6: 2397-404. [46] Tomihata, K. and Ikada, Y., Crosslinking of hyaluronic acid with glutaraldehyde. Journal of Polymer Science Part A Polymer Chemistry, 1997, 35: 3553-3559. [47] Collins, M. and Birkinshaw, C., Physical properties of crosslinked hyaluronic acid hydrogels. J Mater Sci Mater Med, 2008, 19: 3335-3343. [48] Collins, M. and Birkinshaw, C., Comparison of the effectiveness of four different crosslinking agents with hyaluronic acid hydrogel films for tissue-culture applications. Journal of Applied Polymer Science, 2007, 104: 3183-3191. [49] Yoo, H.S., Lee, E.A., Yoon, J.J. and Park, T.G., Hyaluronic acid modified biodegradable scaffolds for cartilage tissue engineering. Biomaterials, 2005, 26: 1925-1933. [50] Ramamurthi, A. and Vesely, I., Evaluation of the matrix-synthesis potential of crosslinked hyaluronan gels for tissue engineering of aortic heart valves. Biomaterials, 2005, 26: 999-1010. [51] Solchaga, L.A., Dennis, J.E., Goldberg, V.M. and Caplan, A.I., Hyaluronic acid-based polymers as cell carriers for tissue-engineered repair of bone and cartilage. Journal of Orthopaedic Research, 1999, 17: 205-213. [52] Dimonie, M., Schell, H.D., Hubca, G., Mateescu, M.A., Oprescu, C.G., Todireanu, S., Maior, O., Languri J. and Iosif, M., Synthesis of beaded polyvinyl-alcohol by suspension methanolysis of polyvinyl acetate - derivatization and some applications. Journal of Macromolecular Science-Chemistry, 1985, A22: 729-754. [53] Hezaveh, H. and Muhamad, I.I., Controlled drug release via minimization of burst release in pH-response kappa-carrageenan/polyvinyl alcohol hydrogels. Chemical Engineering Research and Design, 2013, 91: 508-519. [54] Chuang, W.Y., Young, T.H., Yao, C.H. and Chiu, W.Y., Properties of the poly(vinyl alcohol)/chitosan blend and its effect on the culture of fibroblast in vitro. Biomaterials, 1999, 20: 1479-1487. [55] Burczak, K., Gamian, E. and Kochman, A., Long-term in vivo performance and biocompatibility of poly(vinyl alcohol) hydrogel macrocapsules for hybrid-type artificial pancreas. Biomaterials, 1996, 17: 2351-2356. [56] Young, T.H., Chuang, W.Y., Hsieh, M.Y., Chen, L.W. and Hsu, J.P., Assessment and modeling of poly(vinyl alcohol) bioartificial pancreas in vivo. Biomaterials, 2002, 23: 3465-3501. [57] Paul, W. and Sharma, C.P., Acetylsalicylic acid loaded poly(vinyl alcohol) hemodialysis membranes: effect of drug release on blood compatibility and permeability. Journal of Biomaterials Science, Polymer Edition, 1997, 8: 755-764. [58] Huang, M.H. and Yang, M.C., Evaluation of glucan/poly(vinyl alcohol) blend wound dressing using rat models. International Journal of Pharmaceutics, 2008, 346: 38-46. [59] Nie, S.P., Wang, C., Cui, S.W., Wang, Q., Xie, M.Y. and Phillips, G.O., A further amendment to the classical core structure of gum arabic (Acacia senegal). Food Hydrocolloids, 2013, 31: 42-48. [60] Tiss, A., Carriere F. and Verger, R., Effects of Gum Arabic on Lipase Interfacial Binding and Activity. Analytical Biochemistry, 2001, 294: 36-43. [61] Homayoni, H., Ravandi, S.A.H. and Valizadeh, M., Influence of the Molecular Weight of Chitosan on the Spinnability of Chitosan/Poly(vinyl alcohol) Blend Nanofibers. Journal of Applied Polymer Science, 2009, 113: 2507-2513. [62] Gilman, J.W., VanderHart D.L. and Kashiwagi, T., Thermal Decomposition Chemistry of Poly (vinyl alcohol). Fire and PoLymers II: Materials and Test for Hazard Prevention, ACS Symposium Series, 1994, 599: 21-26. [63] Zhang, L.M., Wu, C.X. and Huang, J.Y., Synthesis and characterization of a degradable composite agarose/HA hydrogel. Carbohydrate Polymers, 2012, 88: 1445–1452. [64] Dong, Y., Ruan, T. and Wang, H., Studies on glass transition temperature of chitosan with four techniques. Journal of Applied Polymer Science, 2004, 93: 1553-1558. [65] Zidan, H.M., Effect of AgNO3 filling and UV-irradiation on the structure and morphology of PVA films. Polymer Testing, 1999, 18: 449–461. [66] Jia, Y.T., Gong, J., Gu, X.H., Kim, H.Y., Dong, J. and Shen, X.Y., Fabrication and characterization of poly (vinyl alcohol)/chitosan blend nanofibers produced by electrospinning method. Carbohydrate Polymers, 2007, 67: 403-409. [67] Charernsriwilaiwat, N., Opanasopit, P., Rojanarata, T., Ngawhirunpat T. and Supaphol, P., Preparation and characterization of chitosan-hydroxybenzotriazole / polyvinyl alcohol blend nanofibers by the electrospinning technique. Carbohydrate Polymers, 2010, 81: 675-680. [68] Majumdar, S. and Adhikari, B., Polyvinyl alcohol: A taste sensing material. Sensors and Actuators B-Chemical, 2006, 114: 747-755. [69] Yue, W., Preparation of low-molecular-weight hyaluronic acid by ozone treatment. Carbohydrate Polymers, 2012, 89: 709–712. [70] Cui, S.W., Phillips, G.O., Blackwell, B. and Nikiforuk, J., Characterisation and properties of Acacia senegal (L.) Willd. var. senegal with enhanced properties (Acacia (sen) SUPERGUMTM): Part 4. Spectroscopic characterisation of Acacia senegal var. senegal and Acacia (sen) SUPERGUMTM arabic. Food Hydrocolloids, 2007, 21: 347-352. [71] Burger, C., Hsiao, B.S. and Chu, B., Nanofibrousmaterials and their applications. Annual Reviews, 2006, 36: 333-368. [72] Ali, B.H., Ziada, A. and Blunden, G., Biological effects of gum arabic: A review of some recent research. Food and Chemical Toxicology, 2009, 47: 1-8. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/60833 | - |
dc.description.abstract | 許多研究已證實,含有透明質酸的複合生醫材料有助於細胞培養。由於幾丁聚醣和透明質酸有著生物相容性高、易於改質等優點,若製成和胞外間質結構相似的奈米纖維,可增加其在生醫領域裡的應用。因此本研究目標為以靜電紡絲法製作透明質酸、幾丁聚醣和聚乙烯醇的三成分複合奈米纖維膜。實驗中以不同濃度和比例的三成分溶液來搭配流量、電壓的改變,來尋找適合靜電紡絲的參數範圍。
根據實驗結果,由電子顯微鏡和黏度分析,可知黏度明顯影響靜電紡絲的製程。熱化學分析結果證實透明質酸和幾丁聚醣為均勻物理性混合,無明顯相分離產生,紅外線吸收光譜也顯示官能基並未有異動。總和以上所述,本研究成功地將透明質酸、幾丁聚醣和聚乙烯醇製成奈米纖維膜,希冀未來可應用於組織工程等相關科技中。 | zh_TW |
dc.description.abstract | Until today, many studies have proven that attachment and proliferation of cells can be enhanced by adding hyaluronan into the experimental materials. With the advantages of good biocompatibility and functional group modification for both chitosan and hyaluronan, fabricating them into nanofibrous structures like extracellular matrix may improve their applications in biomedical field. So the aim of this research is to fabricate hyaluronan/chitosan/polyvinyl alcohol (HA/CS/PVA, or H/C/P) composite nanofibers by electrosinning. In following experiments, the material concentrations, material ratios, feed flow rates and applied voltages were varied to investigate the best parameters for electrospinning.
According to the results, thermal analysis indicated that HA and CS in the fabricated composite nanofibrous mats were mixed homogeneously without obvious phase separation. The spectrum from FT-IR also showed no alteration of functional groups. In this research, the hyaluronan/chitosan/polyvinyl alcohol nanofibrous mats were successfully fabricated. It is expected that such composite mats can be applied in tissue engineering-related fields in the future. | en |
dc.description.provenance | Made available in DSpace on 2021-06-16T10:32:14Z (GMT). No. of bitstreams: 1 ntu-102-R00524052-1.pdf: 13379848 bytes, checksum: 75cbebf0e4fbdb390529631d528033ed (MD5) Previous issue date: 2013 | en |
dc.description.tableofcontents | 誌 謝 I
摘 要 III Abstract V 目 錄 VII 圖目錄 XI 表目錄 XV 符號縮寫說明 XVII 中英文字對照表 XIX 1 緒 論 1 1.1 研究背景與動機 1 1.2 實驗架構與流程 3 2 文獻回顧 5 2.1 組織工程 5 2.2 胞外間質 7 2.3 細胞支架 9 2.4 聚電解質複合物 11 2.5 以靜電紡絲法製作奈米纖維 12 2.5.1 靜電紡絲原理 12 2.5.2 靜電紡絲實驗參數 14 2.5.3 靜電紡絲裝置種類 18 2.5.4 靜電紡絲材料種類 21 2.5.5 幾丁聚醣和透明質酸之靜電紡絲法 22 2.6 幾丁聚醣 24 2.7 透明質酸 26 2.8 聚乙烯醇 28 2.9 天然多醣 29 3 實驗材料、儀器與方法 31 3.1 實驗材料 31 3.2 實驗儀器 32 3.3 實驗方法 33 3.3.1 透明質酸/幾丁聚醣/聚乙烯醇混合溶液配製 33 3.3.2 混合溶液物化性質分析 36 3.3.3 靜電紡絲法 37 3.3.4 奈米纖維之分析 38 4 實驗結果與討論 41 4.1 溶液性質分析 41 4.1.1 黏度 41 4.1.2 導電度 45 4.2 奈米纖維之製備及分析 48 4.2.1 溶液濃度及組成之影響 48 4.2.2 透明質酸分子量之影響 53 4.2.3 聚乙烯醇分子量之影響 64 4.2.4 攪拌時間之影響 70 4.2.5 氯化鈉之影響 75 4.3 熱性質分析 80 4.3.1 TGA 80 4.3.2 DSC 82 4.4 FT-IR 84 4.5 機械性質測試 86 5 結論及未來研究方向 89 5.1 結論 89 5.2 未來研究方向 90 6 參考文獻 91 | |
dc.language.iso | zh-TW | |
dc.title | 以靜電紡絲法製備透明質酸/幾丁聚醣/聚乙烯醇複合奈米纖維之研究 | zh_TW |
dc.title | Fabrication of Hyaluronan/Chitosan/Polyvinyl Alcohol Composite Nanofibers by Electrospinning | en |
dc.type | Thesis | |
dc.date.schoolyear | 101-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 王大銘(Da-Ming Wang),謝子陽 | |
dc.subject.keyword | 透明質酸,幾丁聚醣,聚乙烯醇,靜電紡絲法,戊二醛, | zh_TW |
dc.subject.keyword | Hyaluronan,Chitosan,Polyvinyl Alcohol,Electrospinning,Glutaraldehyde, | en |
dc.relation.page | 98 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2013-08-14 | |
dc.contributor.author-college | 工學院 | zh_TW |
dc.contributor.author-dept | 化學工程學研究所 | zh_TW |
顯示於系所單位: | 化學工程學系 |
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