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| ???org.dspace.app.webui.jsptag.ItemTag.dcfield??? | Value | Language |
|---|---|---|
| dc.contributor.advisor | 蔡偉博 | |
| dc.contributor.author | Chun-Chieh Lin | en |
| dc.contributor.author | 林雋杰 | zh_TW |
| dc.date.accessioned | 2021-06-16T10:20:18Z | - |
| dc.date.available | 2018-08-26 | |
| dc.date.copyright | 2013-08-26 | |
| dc.date.issued | 2013 | |
| dc.date.submitted | 2013-08-16 | |
| dc.identifier.citation | [1] Lysaght MJ, Hazlehurst AL. Tissue engineering: The end of the beginning. Tissue Engineering. 2004;10:309-20.
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/60515 | - |
| dc.description.abstract | 幾丁聚醣 (chitosan)，是將幾丁質去乙醯化的衍生物，它具有好的生物相容性和降解性在組織工程的應用上，常被用來製作支架。本研究的目的在於試圖添加聚乙二醇及羧酸甜菜鹼進入幾丁聚醣支架中，期待它們能改善支架的物理性質，藉由支架上骨母細胞的培養，觀察加入聚乙二醇及羧酸甜菜鹼的支架是否會隊谷母細胞的生長及分化造成影響。
從結果中我們發現，含有聚乙二醇的支架具有更大的孔洞，較差的機械性質及更高的含水量。在細胞實驗中，我們發現含有聚乙二醇的支架對骨母細胞的生長效果有顯著的提升，但是對骨母細胞的分化上無明顯的助益。 在第二部分的實驗中我們發現，在支架中參入羧酸甜菜鹼，會讓支架具有較高的交聯程度，較強的機械性質，及較低的含水量。在細胞實驗中，我們發現加入羧酸甜菜鹼的支架相較於純幾丁聚醣支架，在骨母細胞生長上也有所提升，此外，在骨母細胞的分化上，加入羧酸甜菜鹼的支架明顯優於含有聚乙二醇的支架，因為對分化來講，它具有較好的細胞型態以及不錯的細胞生長。 從此實驗中得知，加入親水性分子像是聚乙二醇及羧酸甜菜鹼進入幾丁聚醣支架中，會增進骨母細胞的生長，但是對骨母細胞的分化並無直接的影響。 | zh_TW |
| dc.description.abstract | Chitosan, the deacetylated derivative of chitin. Because chitosan is biocompatible and biodegradable, so it is a promising scaffold material for tissue engineering applications. In this study, we tried to add poly(ethylene glycol) (PEG) and carboxybetaine (CBMA) to scaffolds and expected they would improve the physical property of chitosan scaffolds. Then we cultured osteoblasts on scaffold to observe the influence of chitosan scaffolds incorporated with poly(ethylene glycol) or carboxybetaine on the proliferation and differentiation of osteoblasts.
As result, we found scaffolds containing PEG exhibited bigger pore size, weaker mechanical properties and higher water content of scaffolds. In cell culture experiment, we observed that the cell proliferation of osteoblasts on CHI/PEG scaffolds were better than the cell proliferation of osteoblasts on pure chitosan scaffolds. However, addition of PEG to scaffolds had no benefit on differentiation of osteoblasts. In second part of study, by characteristic analysis, we found scaffolds containing CBMA exhibited degree of crosslinking, stronger mechanical properties and lower water content of scaffolds. In cell culture experiment, we observed that the cell proliferation of osteoblasts on CHI/CBMA scaffolds were better than the cell proliferation of osteoblasts on pure chitosan scaffolds. In addition, differentiation of osteoblasts of CHI/CBMA scaffolds was much better than that of pure chitosan scaffolds, because of good cell proliferation and cell form. The research presents that adding hydrophilic molecule such as poly(ethylene glycol) (PEG) and carboxybetaine (CBMA) to scaffolds improved the cell proliferation of osteoblasts, however, had no directly influence on differentiation of osteoblast. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-16T10:20:18Z (GMT). No. of bitstreams: 1 ntu-102-R00524073-1.pdf: 1587171 bytes, checksum: 3cd3a9792d04401493a40afcd8fd3943 (MD5) Previous issue date: 2013 | en |
| dc.description.tableofcontents | Abstract ii
List of Tables viii List of Figures ix Chapter 1 1 Introduction 1 1.1 Tissue Engineering 1 1.1.1 Overview of Tissue Engineering 1 1.1.2 Scaffolding in Tissue Engineering 3 1.1.3 Cell culture in the scaffold 5 1.1.4 Application of bone engineering 7 1.2 Osteoblasts 8 1.3 Proliferation and differentiation of osteoblasts 10 1.4 Chitosan 12 1.5 Poly (ethylene glycol), PEG 13 1.6 Carboxybetaine, CBMA 14 1.7 Chitosan scaffold fabrication techniques 14 1.7.1 Freeze drying and crosslinking by azide chemistry 14 1.7.2 Free radical polymerization crosslinking method 16 1.8 Motive and aim 17 1.9 Research frame work 17 Chapter 2 18 Materials and Methods 18 2.1 Materials 18 2.1.1 Chitosan and poly(ethylene glycol) scaffold 18 2.1.2 Chitosan and carboxybetaine scaffold 18 2.1.3 Cell culture 19 2.1.4 Lactate dehydrogenase (LDH) assay 20 2.1.5 Alkaline phosphatase (ALP) activity 20 2.1.6 Mineralization 21 2.1.7 Equipments and experimental materials 21 2.1.8 Solution formula 22 2.2 Methods 24 2.2.1 Preparation of chitosan conjugated with photoreactive groups or peptides 24 2.2.2 Synthesis of chitosan conjugated with methacrylate (CHI-ene) 26 2.2.3 Preparation of chitosan and poly(ethylene glycol) scaffold 26 2.2.4 Preparation of chitosan and carboxybetaine scaffold 27 2.2.5 Characterization of chitosan and poly(ethylene glycol) scaffolds 28 2.2.6 Characterization of chitosan and carboxybetaine scaffolds 30 2.2.7 Cell cultured on chitosan/PEG and chitosan/CBMA scaffolds 31 2.2.8 Cell proliferation (LDH) 32 2.2.9 Alkaline phosphatase (ALP) activity 33 2.2.10 Calcium quantification 33 2.2.11 Mineralization culture of osteoblasts/scaffold constructs 34 2.2.12 Statistic analysis 34 Chapter 3 35 Characterization and cell affinity analysis of chitosan and poly(ethylene glycol) scaffold 35 3.1 Characterization of chitosan and poly(ethylene glycol) scaffolds 35 3.1.1 Effects of poly(ethylene glycol) concentration on pore structure 35 3.1.2 Effects of poly(ethylene glycol)concentration on mechanical properties 36 3.1.3 Effects of poly(ethylene glycol) concentration on water content 37 3.2 Culture of osteoblasts in chitosan and poly(ethylene glycol) scaffolds 37 3.3 Mineralization culture of chitosan and poly(ethylene glycol) scaffolds 39 3.4 Discussion 40 Chapter 4 54 Characterization and cell affinity analysis of chitosan and carboxybetaine scaffold 54 4.1 1H NMR of chitosan and CHI-ene 54 4.2 Characterization of chitosan and carboxybetaine scaffolds 55 4.1.1 Effects of carboxybetaine concentration on mechanical properties 55 4.1.2 Effects of carboxybetaine concentration on water content 56 4.3 Culture of osteoblasts in chitosan and carboxybetaine scaffolds 56 4.4 Mineralization culture of chitosan and carboxybetaine scaffolds 58 4.5 Discussion 59 Chapter 5 71 Conclusion 71 Chapter 6 73 Future Works 73 Reference 74 Appendix 79 | |
| dc.language.iso | en | |
| dc.subject | 骨組織工程 | zh_TW |
| dc.subject | 聚乙二醇 | zh_TW |
| dc.subject | 幾丁聚醣支架 | zh_TW |
| dc.subject | 羧酸甜菜鹼 | zh_TW |
| dc.subject | 骨母細胞 | zh_TW |
| dc.subject | chitosan scaffold | en |
| dc.subject | PEG | en |
| dc.subject | CBMA | en |
| dc.subject | osteoblasts | en |
| dc.subject | bone tissue engineering | en |
| dc.title | 幾丁聚醣支架添加聚乙二醇或羧酸甜菜鹼對於骨母細胞生長及分化的影響 | zh_TW |
| dc.title | The influence of chitosan scaffolds incorporated with poly(ethylene glycol) or carboxybetaine on the proliferation and differentiation of osteoblasts | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 101-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 王孟菊,游佳欣 | |
| dc.subject.keyword | 幾丁聚醣支架,聚乙二醇,羧酸甜菜鹼,骨母細胞,骨組織工程, | zh_TW |
| dc.subject.keyword | chitosan scaffold,PEG,CBMA,osteoblasts,bone tissue engineering, | en |
| dc.relation.page | 82 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2013-08-16 | |
| dc.contributor.author-college | 工學院 | zh_TW |
| dc.contributor.author-dept | 化學工程學研究所 | zh_TW |
| Appears in Collections: | 化學工程學系 | |
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