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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 林乃君 | |
dc.contributor.author | Ya-Hui Chuang | en |
dc.contributor.author | 莊雅惠 | zh_TW |
dc.date.accessioned | 2021-06-16T10:19:57Z | - |
dc.date.available | 2018-08-27 | |
dc.date.copyright | 2013-08-27 | |
dc.date.issued | 2013 | |
dc.date.submitted | 2013-08-16 | |
dc.identifier.citation | 王明仁。2002。塔塔加高山森林生態系中土壤微生物生質量和酸性磷酸酯酶活性與土壤中生物有效性磷含量之關係。國立臺灣大學農業化學研究所碩士論文,臺北,臺灣。
朱亭錚。2007。臺灣草苺栽培之過去與前瞻。國立臺灣大學園藝學研究所碩士論文,臺北,臺灣。 安寶貞、蔡志濃。2009年。植物疫病菌之診斷技術開發。農業生技產業 20: 44–48。 卓昕岑。2002。高海拔塔塔加及低海拔福山森林土壤微生物族群研究。國立臺灣大學農業化學研究所碩士論文,臺北,臺灣。 張廣淼、蔡正賢、吳添益。2007。肥料用量對高架草苺生育及產量之影響。苗栗區農業改良場研究彙報 1: 1–14。 張廣淼、吳添益。2004。有機資材應用於草苺栽培。pp. 59–276。財團法人全方位農業振興基金會,臺北,臺灣。 張廣淼。2004。草苺高架床育苗技術。pp. 1–13。行政院農業委員會苗栗區農業改良場。 張必輝。2002。臺大山地實驗農場梅峰地區不同經歷之土壤肥力特性與其上之植物營養特性。國立臺灣大學農業化學所碩士論文,臺北,臺灣。 隔山普宣、酊田治幸。1990。促成イチゴの循環方式ロックウール栽培における育苗培地,定植法および培養液濃度。德島農試研報 27: 18–28。 楊秋忠、趙維良、廖啟成、黃山內、曾顯雄、許文輝。2003。臺灣土壤微生物之收集應用。中正農業科技社會公益基金會,臺北,臺灣。 潘暉真。2007。荖濃溪和楠梓仙溪流域微生物相之調查。國立中山大學生物科學系碩士論文,高雄,臺灣。 鍾仁賜。2004。草苺之土壤水分與營養管理。2004 果菜健康管理研討會專集 (楊秀珠、林信山編),pp. 213–225。行政院農業委員會農業藥物毒物試驗所,臺中,臺灣。 魏偉勝。2009。不同施肥管理對土壤有機質含量及組成之影響。國立臺灣大學農業化學所碩士論文,臺北,臺灣。 簡宣裕、石信德、林俊義、張明暉、劉邦基。2008。抗輪點病毒轉基因木瓜之生態影響評估-I.土壤微生物族群數與有效性氧份濃度的差異。臺灣農業研究 57: 49–62。 蘇文瀛、高清文。2000。害物管理手冊 (草苺篇)。行政院農業委員會農業藥物毒物試驗所,臺中,臺灣。 鐘珮哲、彭淑貞、張廣淼、楊秀珠、余思葳。2012。草苺病蟲害之發生與管理。行政院農業委員會動植物防疫檢疫局和農業藥物毒物試驗所編。 行政院農業委員會。2011。農業統計年報(100年)。臺北:行政院農業委員會。網址:http://agrstat.coa.gov.tw/sdweb/public/book/Book.aspx。上網日期:2013/2/3。 Adesemoye, A. O. and Kloepper, J.W. 2009. Plant-microbes interactions in enhanced fertilizer-use efficiency. Appl. Microbiol. Biotechnol. 85: 1–12. Altindag, M., Sahin, M., Esitken, A., Ercisli, S., Guleryuz, M., Donmez, M. F. and Sahin, F. 2006. Biological control of brown rot (Moniliana laxa Ehr.) on apricot (Prunus armeniaca L. cv. Hacıhaliloğlu) by Bacillus, Burkholdria, and Pseudomonas application under in vitro and in vivo conditions. Biol. Control 38: 369–372. Bhatt, I. D. and Dhar, U. 2000. Micropropagation of Indian wild strawberry. Plant Cell Tiss. Org. 60: 83–88. Burgess, L. W., Summerell, B. A., Bullock, S., Gott, K. P. and Backhouse, D. 1994. Laboratory manual for Fusarium research. 3rd ed. Dept. Crop Sci., Uni. Sydney/ Royal Botanic Gardens. Chalfoun, N. R., Castagnaro, A. P. and Diaz Ricci, J. C. 2011. Induced resistance activated by a culture filtrate derived from an avirulent pathogen as a mechanism of biological control of anthracnose in strawberry. Biol. Control 58: 319–329. Carroll, J., Pritts, M. and Heidenreich, C. 2011. Production guide for organic strawberries. http://www.nysipm.cornell.edu/organic_guide/strawberry.pdf. NYS IPM Publ. No. 226 v2, 48. New York: Cornell Univ. Castellanos-Morales, V., Villegas-Moreno, J., Vierheilig, H. and Cardenas-Navarro, R. 2012. Nitrogen availability drives the effect of Glomus intraradices on the growth of strawberry (Fragaria x ananassa Duch.) plants. J. Sci. Food Agric. DOI 10.1002/jsfa.5618. Castellanos-Morales, V., Villegas, J., Wendelin, S., Vierheilig, H., Eder, R. and Cardenas-Navarro, R. 2010. Root colonisation by the arbuscular mycorrhizal fungus Glomus intraradices alters the quality of strawberry fruits (Fragaria × ananassa Duch.) at different nitrogen levels. J. Sci. Food Agric. 90: 1774–1782. Cho, C. T. and Moon, B. J. 1984. Studies on the wilt of strawberry caused by Fusarium oxysporum f. sp. fragariae in Korea. Korean J. Plant Protect. 23: 74–81. Elomari, M., Coroler, L., Verhille, S., Izard, D. and Leclerc, H. 1997. Pseudomonas monteilii sp. nov., isolated from clinical specimens. Int. J. Syst. Bacteriol. 47: 846–852. Fang, X., Kuo, J., You, M. P., Finnegan, P. M. and Barbetti, M. J. 2012. Comparative root colonisation of strawberry cultivars Camarosa and Festival by Fusarium oxysporum f. sp. fragariae. Plant Soil: DOI 10.1007/s11104-012-1205-8. Fang, X., You, M. P. and Barbetti, M. J. 2012. Reduced severity and impact of Fusarium wilt on strawberry by manipulation of soil pH, soil organic amendments and crop rotation. Eur. J. Plant Pathol. DOI 10.1007/s10658-012-0042-1. Fang, X. L., Phillips, D., Li, H., Sivasithamparam, K. and Barbetti, M. J. 2011. Severity of crown and root diseases of strawberry and associated fungal and oomycete pathogens in Western Australia. Australas. Plant Pathol. 40: 109–119. Fang, X. L., Phillips, D., Verheyen, G., Li, H., Sivasithamparam, K. and Barbetti, M. J. 2012. Yields and resistance of strawberry cultivars to crown and root diseases in the field, and cultivar responses to pathogens under controlled environ conditions. Phytopathol. Mediterr. 51: 69–84. Fang, X., Phillips, D., Li, H., Sivasithamparam, K. and Barbetti M. J. 2011. Comparisons of virulence of pathogens associated with crown and root diseases of strawberry in Western Australia with special reference to the effect of temperature. Sci. Hort. 131: 39–48. Gu, C.-B., Jiang, L.-L., Wang, K.-Y., Shi, X.-B., Duan, H.-M. and Lin, C.-H. 2010. Induction and characteristics of Fusarium oxysporum f. sp. fragariae ZY-W resistant to tebuconazole. China Agri. Sci. 43: 2897–2904 (in Chinese). Hose, J. E., Lightner, D. V., Redman, R. M. and Danald, D. A. 1984. Observations on the pathogenesis of the imperfect fungus, Fusarium solani, in the California brown shrimp, Penaeus californiensis. J. Invertebr. Pathol. 44: 292–303. Husaini, A. M., Aquil, S., Bhat M., Qadri, T., Kamaluddin, and Abdin, M. Z. 2008. A High-Efficiency Direct Somatic Embryogenesis system for strawberry. J. Crop Sci. Biotech. 11: 107–100. Jetiyanon, K., Fowler, W. D., and Kloepper, J. W. 2003. Broad-spectrum protection against several pathogens by PGPR mixtures under field conditions in Thailand. Plant Dis. 87: 1390–1394. Jiao, Z., Kawamura, Y., Mishima, N., Yang, R., Li, N., Liu, X., and Ezaki, T. 2003. Need to differentiate lethal toxin-producing strains of Burkholderia gladioli, Which cause seere food poisoning: description of B. gladioli pathovar cocovenenans and an emended description of B. gladioli. Microbiol. Immunol. 47: 915–925. Ling, N., Zhang, W., Tan, S., Huang, Q. and Shen, Q. 2012. Effect of the nursery application of bioorganic fertilizer on spatial distribution of Fusarium oxysporum f. sp. niveum and its antagonistic bacterium in the rhizosphere of watermelon. Appl. Soil Ecol. 59: 13–19. Kirad, K. S., Barche, S. and Singh, D. B. 2009. Response of Integrated Nutrient Management in strawberry (Fragaria x ananassa D.). Acta. Hort. 842: 653–656. Ko, C.-Y., Al-Abdulkarim, A. M., Al-Jowid, S. M. and Al-Baiz, A. 2009. An effective disinfection protocol for plant regeneration from shoot tip cultures of strawberry. Afr. J. Biotech. 11: 2611–2615. Maas, J. L. 2004. Diseases of Fruits and Vegetables: Volume II—Strawberry disease management, pp. 441–483. Springer Netherlands. Naruto, F., Hiroyuki, U., Kazuhiro, I., Masaru, M., Minoru, T. and Yoichi, A. 2002. Specific Oligonucleotide Primers Based on Sequences of the 16S-23S rDNA Spacer Region for the Detection of Burkholderia gladioli by PCR. J. Gen. Plant Pathol. 68: 220–224. Pirlak, M. and Kose, M. 2009. Effects of plant growth promoting rhizobacteria on yield and some fruit properties of strawberry. J. Plant Nutr. 32: 1173–1184. Sakila, S., Ahmed, M. B., Roy, U. K., Biswas, M. K., Karim, R., Razvy, M. A., Hossain, M., Islam, R. and Hoque, A. 2007. Micropropagation of Strawberry (Fragaria X ananassa Duch.) A Newly Introduced Crop in Bangladesh. Am.-Eurasian J. Sci. Res. 2: 151–154. Smith, B. J. 2009. Nitrogen fertilizer affects the severity of anthracnose crown rot disease of greenhouse grown strawberries. Plant Health Progress. DOI:10.1094/PHP-2009-0609-01-RS. Stoyanova, M., Pavlina, I., Moncheva, P., and Bogatzevska, N. 2007. Biodiversity and Incidence of Burkholderia Species. Biotechnol. Biotec. Eq. 47: 306–310. Wang, R. J., Cheng, S. H., Liou, Y. T., Chang, C. M., Chang, H. Y. and Chang, S. J. 2010. Standardization of evaluated parameters of strawberry cultivars by principle components analysis techniques. J. Agri. Assoc. Taiwan 11: 105–120. Wu, H., Yang, X., Fan, J., Miao, W., Ling, N., Xu, Y., Huang, Q. and Shen, Q. 2009. Suppression of Fusarium wilt of watermelon by a bio-organic fertilizer containing combinations of antagonistic microorganisms. Bio. Control 54: 287–300. Wang, Z. W., Li, X. Z., Liu, Y. L. and Wang, J. J. 1999. Biological control of strawberry wilt with antagonistic microbes. Chinese J. Bio. Control 15: 187 (in Chinese). Vinee-Prue, D. and Guttridge, C. G. 1973. Floral Initiation in Strawberry: Spectral Evidence for the Regulation of Flowering by Long-day Inhibition. Planta (Berl.) 110: 165–172. Zhang, Y., Fan, T., Jia, W., Zhang, W., Liu, Q., Li, B. and Zhang, L. 2012. Identification and characterization of a Bacillus subtilis strain TS06 as bio-control agent of strawberry replant disease. African J. Biotech. 3: 570–580. Zeng, F. C., Huang, Y., Zhao, Y. Q. and Ma, S. Q. 2006. Biological characteristics of strawberry Fusarium wilt. J. Sichuan Agri. Uni. 24: 156–160 (in Chinese). Zheng L., Zhu, Q. Z., Feng, Z. L. and Huang, J. B. 2006. Biological characteristics and pathogeny identification of strawberry Fusarium wilt. Hubei Agri. Sci. 45: 194–195 (in Chinese). Cornell Univ. 2013. Cornell Fruit. Cornell Univ: Dept. of Horticulture. Available at: http://www.fruit.cornell.edu/berry/production/strawberryproduction.htm. Accessed 3 Febuary 2013. Nicholls, Z., Layden, I., Bagshaw, J., Stockwell, B. and Grobler, L. 2008. Strawberry: best soil, water and nutrient management practices. Queensland: Dept. of Primary Industries and Fisheries. Available at: http://era.deedi.qld.gov.au/1441/1/strawb_final-web3.pdf. Accessed 3 Febuary 2013. Ohio State Univ. 2013. Integrated management of strawberry diseases. Ohio State Univ/OARDC: Dept. of Plant Pathology. Available at: http://www.oardc.ohio-state.edu/fruitpathology/organic/PDF/OSU-Organic-Strawberry-Disease.pdf. Accessed 3 Febuary 2013. Poling, E. B. 2012. Strawberry Plant Structure and Growth Habit. Cornell Univ: Dept. of Horticulture. Available at: http://www.hort.cornell.edu/expo/proceedings/2012/Berries/Berry%20Plant%20Structure%20Poling.pdf. Accessed 3 Febuary 2013. Phillips, D. and Golzar, H. 2008. Strawberry root and crown rot disease survey: 2005 and 2006 seasons. Government of Western Australia, Australia: Dept. of Agriculture and Food. Available at: http://www.agric.wa.gov.au/objtwr/imported_assets/content/hort/fn/cp/strawberries/strawberrydisease_bn08.pdf. Accessed 3 Febuary 2013. Ullio, L. and Macarthur, E. 2004. Strawberry disease control guide. Government of New South Wales, Australia: Dept. of Primary Industries. Available at: http://www.dpi.nsw.gov.au/__data/assets/pdf_file/0011/119558/strawberry-disease-control.pdf.Accessed 3 Febuary 2013. USDA. 2012. Nutrient & Pest management. USDA: Natural Resources Conservation Service. Available at: http://www.nrcs.usda.gov/wps/portal/nrcs/main/national/landuse/crops/npm. Accessed 5 May 2012. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/60503 | - |
dc.description.abstract | 草苺 (Fragaria x ananassa Duch.) 在臺灣屬於高經濟價值果樹之一,近年來發現草苺育苗期植株萎凋死亡之情形相當嚴重。根據近期研究,臺灣未曾記錄之草苺尖鐮孢菌 (Fusarium oxysporum f. sp. fragariae) 為造成草苺萎凋的主因之一,會造成新葉有大小葉的狀況。本研究之目的為發展草苺尖鐮孢菌的控制方法。首先對草苺尖鐮孢菌的病原接種與生長特性進行分析,再將自行篩選出具有拮抗病原菌能力、可能可促進植物生長特性且不具常用藥劑感受性之土壤分離菌株應用於草苺育苗期和本田期病害管理上,評估其維護種植株健康狀態之能力。經過病原性測試後發現,草苺尖鐮孢菌在接種濃度為 1×106 spores/mL 下,發病率及嚴重度為 20% 和 50% 左右,且會隨接種濃度提高而加重。菌絲體較適生長溫度為 28°C,而在酸鹼值為 5.0 時生長較差,與其他酸鹼值下有顯著差異。草苺的最適生長酸鹼值為 5.0 至 6.8,因此,未來或許可利用監控土壤酸鹼度的方式,來降低草苺鐮孢菌的危害。經過選擇性培養基初步篩選,從土壤分離菌株當中,挑選拮抗草苺炭疽及鐮孢菌的 595 和 376 做為主要測試菌株,此二菌株鑑定結果均屬於 Bacillus spp.,依據其拮抗能力,應可降低草苺根系遭受病原侵擾。因此,搭配具有生物防治潛力且能快速增殖之 Trichoderma spp. ML1 和 ML56 共同澆灌於植株根圈環境中來評估其對草苺病害及生長狀況之影響。綜合所有試驗結果顯示,Bacillus spp. 595 能夠有效控制病害發生率,且在短期內促進植株生長,適合於育苗期施用;然而在較為複雜之田間環境中,不論是每週或隔週的澆灌頻率,混合 Bacillus spp. 和 Trichoderma spp. 的處理會有較好的病害控制和促進植株生長效果。本研究直接於草苺產業的重要產區進行田間試驗,其試驗結果極具有產業應用價值與進一步相關研究之參考性。 | zh_TW |
dc.description.abstract | Strawberry is one of the fruits with greater economic profit in Taiwan. Recently, the wilting or death ratio of seedlings and younger plants during its vegetative stage has been found severely high in Miaoli, and the newly reported Fusarium oxysporum f. sp. fragariae is one of the major causing agents of strawberry wilting in Taiwan. Plants have been diagnosed with the typical symptoms of F. oxysporum f. sp. fragariae infection, which are assymetrical plantlets, minor chlorosis and dehydration. The aim of this study is to investigate the pathogenicity and pysiological characteristics of F. oxysporum f. sp. fragariae, and the effects of soil microbes with antagonistic, plant growth-promoting and pesticide-insensitive activities on the disease incidence during vegetative and fruiting stages of strawberry growting in a nethouse or in the field. The results indicate that the incidence and severity of Fusarium wilt of strawberry inoculated with 1×106 spores/mL under greenhouse environment were around 20% and 50%, respectively, and both increased as the inoculum concentration went up. F. oxysporum f. sp. fragariae prefers 28°C and grows poorly at pH 5.0. The optimum pH for strawberry is 5.0 to 6.8, so we might be able to decrease the disease incidence of strawberry wilt by monitoring the pH value of soil or substrate. Bacillus spp. 595 and 376 were selected for evaluation and the results showed that either in a pot assay or field application, 595 is relatively suitable to be applied during the vegetative stage and combination of Bacillus spp. and Trichoderma spp. has better disease-control and plant growth-promoting effects in the field condition. | en |
dc.description.provenance | Made available in DSpace on 2021-06-16T10:19:57Z (GMT). No. of bitstreams: 1 ntu-102-R00645004-1.pdf: 3749833 bytes, checksum: f457fd08b4557c4fbc66fbf6424f7441 (MD5) Previous issue date: 2013 | en |
dc.description.tableofcontents | 摘要 I
Abstract II 圖目錄 V 表目錄 VI 壹、前言 1 貳、前人研究 4 一、臺灣苗栗縣草苺栽培與病蟲害管理模式 4 二、草苺尖鐮孢菌之蔓延、傳播 8 三、土壤微生物之應用 10 四、研究動機與目的 11 參、材料與方法 12 一、促進植物生長根棲細菌之分離及其特性分析 12 二、草苺尖鐮孢菌病原性與生理特性之分析 19 三、苗栗區農業改良場網室育苗期盆栽試驗 22 四、苗栗區農業改良場網室本田期盆栽試驗 27 五、苗栗縣大湖鄉甜美觀光草苺園本田期田間試驗 30 六、統計分析 33 肆、結果 34 一、土壤分離菌株之特性分析及鑑定 34 二、草苺尖鐮孢菌之病原接種分析與生長特性 42 三、網室育苗期盆栽試驗之土壤分離菌株處理對草苺植株病害控制 46 四、網室本田期盆栽試驗之土壤分離菌株處理對草苺植株病害控制 48 五、本田期田間試驗之土壤分離菌株處理對草苺植株病害控制 51 六、網室育苗期盆栽試驗之土壤分離菌株處理對促進草莓植株的生長效果 63 七、網室本田期盆栽試驗之土壤分離菌株處理對促進草莓植株的生長效果 68 八、本田期田間試驗之土壤分離菌株處理對促進草莓植株的生長效果 74 伍、討論 79 一、土壤分離菌株對草莓萎凋病之病害控制效果 79 二、土壤分離菌株對草莓植株之促進生長效果 85 陸、結論 87 柒、參考文獻 88 捌、附錄 96 | |
dc.language.iso | zh-TW | |
dc.title | 臺灣土壤分離菌株 Bacillus spp. 對草苺萎凋病害防治效果之研究 | zh_TW |
dc.title | The disease-control effect of the Bacillus spp. isolated from soil in Taiwan on pathogenic Fusarium wilt of strawberry | en |
dc.type | Thesis | |
dc.date.schoolyear | 101-2 | |
dc.description.degree | 碩士 | |
dc.contributor.coadvisor | 鍾仁賜 | |
dc.contributor.oralexamcommittee | 廖乾華,葉信宏,鍾嘉綾 | |
dc.subject.keyword | 草苺,草苺尖鐮孢菌,土壤微生物,病害抑制效果, | zh_TW |
dc.subject.keyword | Fragaria x ananassa Duch. (strawberry),Fusarium oxysporum f. sp. fragariae,soil microbes,disease-control effect,plant growth-promoting effect, | en |
dc.relation.page | 104 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2013-08-16 | |
dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
dc.contributor.author-dept | 植物醫學碩士學位學程 | zh_TW |
顯示於系所單位: | 植物醫學碩士學位學程 |
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