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http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/60420完整後設資料紀錄
| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 黃慶璨(Ching-Tsan Huang) | |
| dc.contributor.author | Cheng-Hung Lu | en |
| dc.contributor.author | 呂承鴻 | zh_TW |
| dc.date.accessioned | 2021-06-16T10:17:44Z | - |
| dc.date.available | 2014-02-01 | |
| dc.date.copyright | 2013-08-26 | |
| dc.date.issued | 2013 | |
| dc.date.submitted | 2013-08-16 | |
| dc.identifier.citation | 第七章 參考文獻
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/60420 | - |
| dc.description.abstract | 異源基因表達為現今生技產業中一項重要的平台技術，但異源基因表達量會受許多因素影響，例如:宿主與異源基因密碼子偏性差異大小、宿主是否具有轉譯後修飾作用、啟動子的強度、轉形株的拷貝數、誘導培養條件是否最適化等。
嗜甲醇酵母菌異源表達系統如Pichia pastoris，具有簡易的分生操作、成熟的醱酵技術、良好蛋白質表現量及具轉譯後修飾作用等特點，為目前應用最廣泛的真核表達系統之一。本研究針對P. pastoris表達載體上的訊息胜肽alpha-factor，為來自Saccharomyces cerevisiae交配因子，探討其核酸序列密碼子最適化是否有助於提升外泌蛋白質表現量。以木聚醣酶xyn11B’做為目標基因，研究結果顯示，具有優化訊息胜肽alpha-factor的轉形株，其活性顯著提升，顯示此策略可以提升異源蛋白質表現量。 | zh_TW |
| dc.description.provenance | Made available in DSpace on 2021-06-16T10:17:44Z (GMT). No. of bitstreams: 1 ntu-102-R00b22041-1.pdf: 1630874 bytes, checksum: 160ad856f829473f69f2a5decc1914e5 (MD5) Previous issue date: 2013 | en |
| dc.description.tableofcontents | 謝誌 Ⅰ
摘要 Ⅱ Abstract Ⅲ 目錄 Ⅳ 表目錄 Ⅶ 圖目錄 Ⅷ 第一章 前言 1 1. 基因工程 1 2. 異源表達系統 1 2.1 原核表達系統 1 2.2 真核表達系統 2 3. 嗜甲醇酵母菌表達系統 3 3.1 Pichia pastoris 7 3.2 Alpha-factor 9 4. 瘤胃真菌Neocallimastix frontalis的木聚醣酶 13 5. 影響P. pastoris異源蛋白質表現量 16 5.1 菌株表現型 16 5.2 同義密碼子偏性 16 5.3 異源基因拷貝數 17 5.4 啟動子 18 5.5 訊息胜肽 18 5.6 培養和誘導 19 6. 研究動機與目的 20 第二章 材料與方法 22 1. 菌株與培養條件 22 2. 培養基 22 3. 表現質體建構 24 4. 引子 29 5. 質體轉形 30 6. 轉形株DNA分析 31 7. 轉形株篩選 33 8. 三角瓶培養及生產 33 9. 胞內蛋白質萃取 34 10. 木聚醣酶活性測定 35 11. 西方墨點法 37 12. 即時定量聚合酶鏈鎖反應 39 第三章 結果 40 1. α-factor和xyn11B’密碼子最適化 40 2. 表現載體建構 45 3. 轉形及抗生素濃度篩選 47 4. P.pastoris轉形株染色體分析 49 5. P.pastoris轉形株試管培養分析 50 6. P.pastoris轉形株拷貝數測定 53 7. P.pastoris轉形株搖瓶培養分析 56 8. P.pastoris胞內酵素活性 58 9. SDS-PAGE與西方墨點法分析 60 第四章 討論 62 1. 密碼子使用率 62 2. 分泌路徑 63 3. 轉形株抗藥性 63 4. 轉基因拷貝數 64 5. 胞外和胞內表現量 65 第五章 結論 66 第六章 未來展望 67 第七章 參考文獻 68 | |
| dc.language.iso | zh-TW | |
| dc.title | 利用密碼子最適化的α-factor提升P. pastoris異源蛋白質外泌表現量 | zh_TW |
| dc.title | The use of codon optimized alpha-factor to enhance the
secretion of heterologous protein in Pichia pastoris | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 101-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 許瑞祥(Ruey-Shyang Hseu),楊啟伸(Chii-Shen Yang) | |
| dc.subject.keyword | 嗜甲醇酵母菌,拷貝數,密碼子最適化, | zh_TW |
| dc.subject.keyword | methylotrophic yeasts,copy number,codon optimization, | en |
| dc.relation.page | 73 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2013-08-17 | |
| dc.contributor.author-college | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 生化科技學系 | zh_TW |
| 顯示於系所單位： | 生化科技學系 | |
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