請用此 Handle URI 來引用此文件:
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/60353
完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 嚴震東(Chen-Tung Yen) | |
dc.contributor.author | Wan-Ting Zhao | en |
dc.contributor.author | 趙婉婷 | zh_TW |
dc.date.accessioned | 2021-06-16T10:16:09Z | - |
dc.date.available | 2016-08-26 | |
dc.date.copyright | 2013-08-26 | |
dc.date.issued | 2013 | |
dc.date.submitted | 2013-08-18 | |
dc.identifier.citation | Aoki I, Tanaka C, Takegami T, Ebisu T, Umeda M, Fukunaga M, Fukuda K, Silva AC, Koretsky AP, Naruse S (2002) Dynamic activity-induced manganese-dependent contrast magnetic resonance imaging (DAIM MRI). Magnetic resonance in medicine : official journal of the Society of Magnetic Resonance in Medicine / Society of Magnetic Resonance in Medicine 48:927-933.
Aoki I, Wu YJ, Silva AC, Lynch RM, Koretsky AP (2004) In vivo detection of neuroarchitecture in the rodent brain using manganese-enhanced MRI. NeuroImage 22:1046-1059. Baliki MN, Geha PY, Apkarian AV, Chialvo DR (2008) Beyond feeling: chronic pain hurts the brain, disrupting the default-mode network dynamics. The Journal of neuroscience : the official journal of the Society for Neuroscience 28:1398-1403. Canals S, Beyerlein M, Keller AL, Murayama Y, Logothetis NK (2008) Magnetic resonance imaging of cortical connectivity in vivo. NeuroImage 40:458-472. Cao YQ (2006) Voltage-gated calcium channels and pain. Pain 126:5-9. Chuang KH, Belluscio L, Koretsky AP (2010) In vivo detection of individual glomeruli in the rodent olfactory bulb using manganese enhanced MRI. NeuroImage 49:1350-1356. Eschenko O, Canals S, Simanova I, Beyerlein M, Murayama Y, Logothetis NK (2010) Mapping of functional brain activity in freely behaving rats during voluntary running using manganese-enhanced MRI: implication for longitudinal studies. NeuroImage 49:2544-2555. Eschenko O, Evrard HC, Neves RM, Beyerlein M, Murayama Y, Logothetis NK (2012) Tracing of noradrenergic projections using manganese-enhanced MRI. NeuroImage 59:3252-3265. Farmer MA, Baliki MN, Apkarian AV (2012) A dynamic network perspective of chronic pain. Neuroscience letters 520:197-203. Francis JT, Xu S, Chapin JK (2008) Proprioceptive and cutaneous representations in the rat ventral posterolateral thalamus. Journal of neurophysiology 99:2291-2304. Fukuda J, Kawa K (1977) Permeation of manganese, cadmium, zinc, and beryllium through calcium channels of an insect muscle membrane. Science 196:309-311. Gobbo OL, Petit F, Gurden H, Dhenain M (2012) In vivo detection of excitotoxicity by manganese-enhanced MRI: comparison with physiological stimulation. Magnetic resonance in medicine : official journal of the Society of Magnetic Resonance in Medicine / Society of Magnetic Resonance in Medicine 68:234-240. Grunecker B, Kaltwasser SF, Zappe AC, Bedenk BT, Bicker Y, Spoormaker VI, Wotjak CT, Czisch M (2013) Regional specificity of manganese accumulation and clearance in the mouse brain: implications for manganese-enhanced MRI. NMR in biomedicine 26:542-556. Hu TC, Pautler RG, MacGowan GA, Koretsky AP (2001) Manganese-enhanced MRI of mouse heart during changes in inotropy. Magnetic resonance in medicine : official journal of the Society of Magnetic Resonance in Medicine / Society of Magnetic Resonance in Medicine 46:884-890. Inui T, Inui-Yamamoto C, Yoshioka Y, Ohzawa I, Shimura T (2011) Activation of projective neurons from the nucleus accumbens to ventral pallidum by a learned aversive taste stimulus in rats: a manganese-enhanced magnetic resonance imaging study. Neuroscience 177:66-73. Itoh K, Sakata M, Watanabe M, Aikawa Y, Fujii H (2008) The entry of manganese ions into the brain is accelerated by the activation of N-methyl-D-aspartate receptors. Neuroscience 154:732-740. Iwata M, LeBlanc BW, Kadasi LM, Zerah ML, Cosgrove RG, Saab CY (2011) High-frequency stimulation in the ventral posterolateral thalamus reverses electrophysiologic changes and hyperalgesia in a rat model of peripheral neuropathic pain. Pain 152:2505-2513. Kane PA, Ayton V, Walters HL, Benjamin I, Heaton ND, Williams R, Karani JB (1997) MnDPDP-enhanced MR imaging of the liver. Correlation with surgical findings. Acta Radiol 38:650-654. Kim SK, Nabekura J (2011) Rapid synaptic remodeling in the adult somatosensory cortex following peripheral nerve injury and its association with neuropathic pain. The Journal of neuroscience : the official journal of the Society for Neuroscience 31:5477-5482. Lauterbur PC (1973) Image Formation by Induced Local Interactions: Examples Employing Nuclear Magnetic Resonance. Nature 242:190-191. Lin YJ, Koretsky AP (1997) Manganese ion enhances T1-weighted MRI during brain activation: an approach to direct imaging of brain function. Magnetic resonance in medicine : official journal of the Society of Magnetic Resonance in Medicine / Society of Magnetic Resonance in Medicine 38:378-388. Luo L, Xu H, Li Y, Du Z, Sun X, Ma Z, Hu Y (2012) Manganese-enhanced MRI optic nerve tracking: effect of intravitreal manganese dose on retinal toxicity. NMR in biomedicine 25:1360-1368. Narita K, Kawasaki F, Kita H (1990) Mn and Mg influxes through Ca channels of motor nerve terminals are prevented by verapamil in frogs. Brain research 510:289-295. OTSU N (1979) A Threshold Selection Method from Gray-Level Histograms. Systems, Man and Cybernetics, IEEE Transactions on 9:62-66. Pautler RG, Koretsky AP (2002) Tracing odor-induced activation in the olfactory bulbs of mice using manganese-enhanced magnetic resonance imaging. NeuroImage 16:441-448. Pautler RG, Mongeau R, Jacobs RE (2003) In vivo trans-synaptic tract tracing from the murine striatum and amygdala utilizing manganese enhanced MRI (MEMRI). Magnetic resonance in medicine : official journal of the Society of Magnetic Resonance in Medicine / Society of Magnetic Resonance in Medicine 50:33-39. Paxino G, Watson C (2007) The Brain in Stereotaxic Coordinates. Academic Press, New York, ISBN0123741211. Porreca F, Ossipov MH, Gebhart GF (2002) Chronic pain and medullary descending facilitation. Trends in neurosciences 25:319-325. Saab CY (2012) Pain-related changes in the brain: diagnostic and therapeutic potentials. Trends in neurosciences 35:629-637. Silva AC, Lee JH, Wu CW, Tucciarone J, Pelled G, Aoki I, Koretsky AP (2008) Detection of cortical laminar architecture using manganese-enhanced MRI. Journal of neuroscience methods 167:246-257. Sloot WN, Gramsbergen JB (1994) Axonal transport of manganese and its relevance to selective neurotoxicity in the rat basal ganglia. Brain research 657:124-132. Tucciarone J, Chuang KH, Dodd SJ, Silva A, Pelled G, Koretsky AP (2009) Layer specific tracing of corticocortical and thalamocortical connectivity in the rodent using manganese enhanced MRI. NeuroImage 44:923-931. van der Zijden JP, Wu O, van der Toorn A, Roeling TP, Bleys RL, Dijkhuizen RM (2007) Changes in neuronal connectivity after stroke in rats as studied by serial manganese-enhanced MRI. NeuroImage 34:1650-1657. Watanabe T, Frahm J, Michaelis T (2004) Functional mapping of neural pathways in rodent brain in vivo using manganese-enhanced three-dimensional magnetic resonance imaging. NMR in biomedicine 17:554-568. Watanabe T, Natt O, Boretius S, Frahm J, Michaelis T (2002) In vivo 3D MRI staining of mouse brain after subcutaneous application of MnCl2. Magnetic resonance in medicine : official journal of the Society of Magnetic Resonance in Medicine / Society of Magnetic Resonance in Medicine 48:852-859. Watanabe T, Radulovic J, Boretius S, Frahm J, Michaelis T (2006) Mapping of the habenulo-interpeduncular pathway in living mice using manganese-enhanced 3D MRI. Magnetic resonance imaging 24:209-215. Wendland MF (2004) Applications of manganese-enhanced magnetic resonance imaging (MEMRI) to imaging of the heart. NMR in biomedicine 17:581-594. Woolf CJ (2011) Central sensitization: implications for the diagnosis and treatment of pain. Pain 152:S2-15. Yang PF, Chen DY, Hu JW, Chen JH, Yen CT (2011) Functional tracing of medial nociceptive pathways using activity-dependent manganese-enhanced MRI. Pain 152:194-203. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/60353 | - |
dc.description.abstract | 順磁性的錳離子其通透鈣離子通道之特性,使其可進入活化的神經細胞並順向經由運輸蛋白與突觸小泡傳輸到後突觸細胞。透過量測不同腦區的T1 影像強度可得知各腦區的不同活化程度。這些特徵使得錳離子成為在神經迴路造影研究上一有利的工具。本研究在建立一個慢性埋管給予錳離子的方法來探測神經網路的可塑性變化。首先測試錳離子注射之穩定性及急性神經毒性,實驗顯示等濃度錳離子注射之容積與影像體素大小呈一線性相關。比較初級感覺皮質前掌區(S1FL)之誘發電位振幅在局部注射同側視丘腹後核側邊(VPL) 40 mM 0.5 μl之錳離子後無顯著差異。其次使用長期埋管的動物在單次錳離子注射後觀察錳離子在腦內的清除狀況,實驗顯示腦內錳離子清除半衰期約為26.57小時。最後使用長期埋管在VPL測試40 mM, 0.5 μl之低劑量錳離子是否可鑑別傷害性疼痛刺激,實驗顯示此劑量可成功分辨由福馬林前掌注射之周邊感覺刺激或是食鹽水前掌注射組。以上實驗顯示0.5 μl, 40 mM錳離子以8天以上的間隔可以在慢性埋管中重複施打於腦中來探測神經網路的長期變化。 | zh_TW |
dc.description.abstract | The paramagnetic Mn2+ enters voltage-gated calcium channels and transported anterogradly in active neurons. Using these advantages, neuronal activity in different brain regions can be estimated in manganese-enhanced magnetic resonance imaging (MEMRI). The present study tested the injection constancy, toxicity, clearance rate and detectability of intracranial manganese ion to establish a longitudinal MEMRI protocol. In acute experiment, first we found with constant concentration solution, injected volumes linearly correlated to the voxel numbers in the T1 images. Next we estimated the acute Mn2+ toxicity by recording evoked field potentials in the primary somatosensory cortex (SI) and injecting 40 mM, 0.5 μl Mn2+ solutions in the ventroposterior thalamic nucleus (VPL). The amplitudes showed no significantly differences after Mn2+ administration. In chronic implanted animals, images acquired in different time intervals after a single 0.5 μl, 40 mM Mn2+ injection showed a clearance rate in a half-life of 26.57 hours. For a functional test, we used forepaw formalin pain model. At the dosage of 40 mM and 0.5 μl, using a counter-balanced repetitive Mn2+ injection design, we found transported Mn2+ signal in the SI could be clearly differentiated from saline control. These result indicated 40 mM 0.5μl Mn2+ can be used for longitudinal MEMRI method to test long-term change in neural circuit plasticity. | en |
dc.description.provenance | Made available in DSpace on 2021-06-16T10:16:09Z (GMT). No. of bitstreams: 1 ntu-102-R00b41004-1.pdf: 2088219 bytes, checksum: 3b5b0e76a17688e07637eff5d05469b5 (MD5) Previous issue date: 2013 | en |
dc.description.tableofcontents | Acknowledgements iii
Abstract v 1.Introduction 2 1.1. Chronic pain reshapes the brain circuitry 2 1.2. Brain functional imaging 3 1.3. Longitudinal study on pain pathway tracing 5 1.4. Purpose of this study 5 2. Materials and methods 7 2.1. MnCl2 solution preparation 7 2.1.1.Injection Reproducibility 7 2.1.2.Acute toxicity test by electrophysiological examination 8 2.1.3.Mn2+ clearance in the brain 9 2.1.4.Functional test of repeatedly Mn2+ injections 10 2.2. MRI acquisition and protocol 12 2.3. Image Analysis 13 2.4. Histology 15 3.Results 16 3.1. Mn2+ injected voxel numbers linearly correlated with injected dose 16 3.2. 0.5 μl of 40 mM Mn2+ injection caused little acute damage in the thalamus 16 3.3. Microinjected Mn2+ was cleared at a half-life of 26.57 hour 18 3.4. 40 mM and 0.5 μl of single Mn2+ injection in the VPL could successfully differentiate nociceptive VP-S1 response 18 4.Discussions 21 5.Conclusion 25 Reference 26 Figures 30 | |
dc.language.iso | en | |
dc.title | 錳離子增益性磁振造影法之大鼠長期觀測研究方法探討 | zh_TW |
dc.title | A Methodological Study of Longitudinal Manganese-Enhanced Magnetic Resonance Imaging in the Rat | en |
dc.type | Thesis | |
dc.date.schoolyear | 101-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 張程,陳志宏,陳德祐,林慶波 | |
dc.subject.keyword | 錳離子增益性磁振造影,視丘下侧,腹核,感覺皮質前肢區,長程觀察研究,疼痛, | zh_TW |
dc.subject.keyword | MEMRI,ventroposterior thalamic nucleus,forelimb of somatosensory cortex,longitudinal study,pain, | en |
dc.relation.page | 40 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2013-08-19 | |
dc.contributor.author-college | 生命科學院 | zh_TW |
dc.contributor.author-dept | 動物學研究所 | zh_TW |
顯示於系所單位: | 動物學研究所 |
文件中的檔案:
檔案 | 大小 | 格式 | |
---|---|---|---|
ntu-102-1.pdf 目前未授權公開取用 | 2.04 MB | Adobe PDF |
系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。