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標題: | 針對精神分裂症患者週邊血液內七個微小核醣核酸的表現程度在急性期及部分緩解期之比較 Peripheral Blood Levels of Seven MicroRNAs from Acute to Partial Remission in Schizophrenia |
作者: | Su-Yin Lee 李素音 |
指導教授: | 陳為堅(Wei-Jian Chen) |
關鍵字: | 精神分裂症,微小核醣核酸,周邊血液, Schizophrenia,peripheral blood,microRNA, |
出版年 : | 2013 |
學位: | 碩士 |
摘要: | 過去的研究已證實微小核醣核酸無論在精神分裂症患者的腦部或是週邊血液內都有異常表現,而高度安全、低侵入性的週邊血液檢體內的微小核醣核酸表現被認為是非常具有潛力可發展成有效的臨床生物指標。先前研究從週邊血液內找出七個異常表現的微小核醣核酸,包括miR-34a、miR-449a、miR-564、miR-432、miR-548d、miR-572和miR-652。但對於這七個微小核醣核酸的異常表現是否在精神分裂症不同的發病階段會保持穩定或是會隨著疾病嚴重程度而變動則有待研究。
此篇研究主要目的為:1) 比較精神分裂症患者在急性期入院時與部分緩解出院時兩個階段內的微小核醣核酸表現是否有變化及 2) 再驗證這七個週邊血液內的微小核醣核酸表現量和精神分裂症的相關。研究對象包括48位精神分裂症住院病患及37位性別年齡配對健康對照。病患的血液檢體分別於急性期入院時及部分緩解出院時各抽取一次(健康自願者兩次血液抽取時間為間隔兩個月)。本研究以ABI PRISM 7900 Real Time PCR system定量微小核醣核酸的表現量,再各自比較兩組對象在兩階段內及兩組之間的七個微小核醣核酸表現差異。研究結果發現,精神病患在住院到出院至少兩個月的時間內其七個微小核醣核酸表現量都趨向穩定無太大變化;健康對照組在兩個月追蹤期間內只有miR-548d表現量有顯著增加;比較健康對照和病患的表現量發現,miR-34a, miR-449a, miR-548d, miR-572有達到顯著差異。除了miR-432,其它六個微小核醣核酸和過去研究結果都一致為上調節,此結果懷疑微小核醣核酸的表現量有可能和病程的長短有關。此七個微小核醣核酸被證實在周邊血液內有穩定的表現量,這更加強了周邊血液的微小核醣核酸能夠被當成生物指標的潛力,但是否只對於精神分裂症有特異性及是否在長期追蹤的狀態下依舊表現穩定則需要進一步追蹤探討。 Aberrant expression levels of seven microRNAs (miRNAs), including miR-34a, miR-449a, miR-564, miR-432, miR-548d, miR-572, and miR-652, in peripheral blood were associated with schizophrenia in a previous study. However, it remains unknown whether the expression profiles of the seven-miRNAs remain stable over the clinical course of schizophrenia. This study aimed to cross-validate the association of the seven-miRNAs signature with schizophrenia and to compare the expression change in peripheral levels of the seven-miRNAs from acute to partial remission in inpatients with schizophrenia. A total of 48 patients with schizophrenia and 37 healthy controls were enrolled and their peripheral blood mononuclear cells were collected respectively both at admission (acute state/baseline, T1) and discharge (partial remission / at least two months interval follow-up, T2). In this study, we found that peripheral blood levels of seven-miRNAs signature seem stable from T1 to T2 in schizophrenia inpatients and healthy controls, except for miR-548d in healthy controls. The expression levels of miR-34a, miR-449a, miR-548d and miR-572 were significantly up-regulated when comparing schizophrenia patients with healthy controls, which successfully replicated previous study. However, miR-432 was down-regulated and suspected the expression profiles associate with illness duration. The results suggested that the seven-miRNAs in peripheral blood cells were stable over the clinical course of schizophrenia and it might be served as potential biomarkers for diagnosis of schizophrenia illness. Collectively, the mononuclear leukocyte-based miRNA profiling is an effective way to identify the candidate biomarkers for schizophrenia. |
URI: | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/60257 |
全文授權: | 有償授權 |
顯示於系所單位: | 流行病學與預防醫學研究所 |
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