嗜熱性真菌 Myceliophthora heterothallica 之化學成分及其生物活性研究

Abstract

本研究的目的在於尋找真菌Myceliophthora heterothallica中具有生物活性 的天然物，並且期望可以得到過去文獻未發現的新化合物，Myceliophthora heterothallica 的培養溫度為45℃，初步的活性測試顯示此真菌的乙酸乙酯萃取物有良好的抗癌和抗菌活性，為了瞭解Myceliophthora heterothallica 具有生物活性的化合物為何，在研究中以大量培養的方式以取得較為大量的萃取物，之後再以不同的純化方式進行純化，並以質譜和核磁共振的方式解出化合物的結構。

研究中得到了十二個化合物，其中包含了十一個已知化合物及一個先前未被發表的化合物。而除了分離和純化之外，這些純化後的化合物進行了包括了抗癌活性和抗菌活性生物活性的測試， 實驗結果發現其中有一個化合物具有抗菌的活性是先前未被報導過的，之後這些化合物的其他生物活性或是作用機制或許可以在之後的研究中繼續探討。

The aim of this research is to search for bioactive secondary metabolites from the fungus Myceliophthora heterothallica in order to obtain compounds not previously reported in the literature. Myceliophthora heterothallica was cultured at 45 ℃. Preliminary bioactivity tests showed that the EtOAc crude extract showed good bioactivity on anti-cancer and anti-bacteria bioassays. In order to identify the bioactive compounds of Myceliophthora heterothallica, the fungi was large-scale cultured to obtain large amounts of fungi extract. After extraction, various purification methods (gel filtration, SiO2 open column, HPLC) were applied to purify the bioactive compounds and the purified compounds were identified by NMR and mass spectroscopy. There were twelve compounds obtained in this study, including eleven known and one compound never reported previously. In addition to purification of compounds, anti-bacteria and anti-cancer bioactivity was tested. A known compound was shown to exhibit previously unreported anti-bacterial activity which warrants further research into its mechanism and biochemistry.