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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 李士傑 | |
dc.contributor.author | Wen-Lu Chung | en |
dc.contributor.author | 鍾文祿 | zh_TW |
dc.date.accessioned | 2021-06-16T09:55:21Z | - |
dc.date.available | 2022-02-08 | |
dc.date.copyright | 2017-02-08 | |
dc.date.issued | 2016 | |
dc.date.submitted | 2016-12-29 | |
dc.identifier.citation | References
Ablain, J., E. M. Durand, S. Yang, Y. Zhou and L. I. Zon (2015). 'A CRISPR/Cas9 vector system for tissue-specific gene disruption in zebrafish.' Dev Cell 32(6): 756-764. Araya, C., L. C. Ward, G. C. Girdler and M. Miranda (2016). 'Coordinating cell and tissue behavior during zebrafish neural tube morphogenesis.' Dev Dyn 245(3): 197-208. Bachner, D., M. Ahrens, N. Betat, D. Schroder and G. Gross (1999). 'Developmental expression analysis of murine autotaxin (ATX).' Mech Dev 84(1-2): 121-125. Barbayianni, E., E. Kaffe, V. Aidinis and G. Kokotos (2015). 'Autotaxin, a secreted lysophospholipase D, as a promising therapeutic target in chronic inflammation and cancer.' Prog Lipid Res 58: 76-96. Bernardos, R. L. and P. A. Raymond (2006). 'GFAP transgenic zebrafish.' Gene Expr Patterns 6(8): 1007-1013. Bradford, Y., T. Conlin, N. Dunn, D. Fashena, K. Frazer, D. G. Howe, J. Knight, P. Mani, R. Martin, S. A. Moxon, H. Paddock, C. Pich, S. Ramachandran, B. J. Ruef, L. Ruzicka, H. Bauer Schaper, K. Schaper, X. Shao, A. Singer, J. Sprague, B. Sprunger, C. Van Slyke and M. Westerfield (2011). 'ZFIN: enhancements and updates to the Zebrafish Model Organism Database.' Nucleic Acids Res 39(Database issue): D822-829. Choi, J. W., D. R. Herr, K. Noguchi, Y. C. Yung, C. W. Lee, T. Mutoh, M. E. Lin, S. T. Teo, K. E. Park, A. N. Mosley and J. Chun (2010). 'LPA receptors: subtypes and biological actions.' Annu Rev Pharmacol Toxicol 50: 157-186. Ciruna, B., A. Jenny, D. Lee, M. Mlodzik and A. F. Schier (2006). 'Planar cell polarity signalling couples cell division and morphogenesis during neurulation.' Nature 439(7073): 220-224. De Marco, P., E. Merello, G. Piatelli, A. Cama, Z. Kibar and V. Capra (2014). 'Planar cell polarity gene mutations contribute to the etiology of human neural tube defects in our population.' Birth Defects Res A Clin Mol Teratol 100(8): 633-641. Estivill-Torrus, G., P. Llebrez-Zayas, E. Matas-Rico, L. Santin, C. Pedraza, I. De Diego, I. Del Arco, P. Fernandez-Llebrez, J. Chun and F. R. De Fonseca (2008). 'Absence of LPA1 signaling results in defective cortical development.' Cereb Cortex 18(4): 938-950. Fotopoulou, S., N. Oikonomou, E. Grigorieva, I. Nikitopoulou, T. Paparountas, A. Thanassopoulou, Z. Zhao, Y. Xu, D. L. Kontoyiannis, E. Remboutsika and V. Aidinis (2010). 'ATX expression and LPA signalling are vital for the development of the nervous system.' Dev Biol 339(2): 451-464. Fukushima, N. (2004). 'LPA in neural cell development.' J Cell Biochem 92(5): 993-1003. Geldmacher-Voss, B., A. M. Reugels, S. Pauls and J. A. Campos-Ortega (2003). 'A 90-degree rotation of the mitotic spindle changes the orientation of mitoses of zebrafish neuroepithelial cells.' Development 130(16): 3767-3780. Girdler, G. C., C. Araya, X. Ren and J. D. Clarke (2013). 'Developmental time rather than local environment regulates the schedule of epithelial polarization in the zebrafish neural rod.' Neural Dev 8: 5. Goto, T., L. Davidson, M. Asashima and R. Keller (2005). 'Planar cell polarity genes regulate polarized extracellular matrix deposition during frog gastrulation.' Curr Biol 15(8): 787-793. Greenman, R., A. Gorelik, T. Sapir, J. Baumgart, V. Zamor, M. Segal-Salto, S. Levin-Zaidman, V. Aidinis, J. Aoki, R. Nitsch, J. Vogt and O. Reiner (2015). 'Non-cell autonomous and non-catalytic activities of ATX in the developing brain.' Front Neurosci 9: 53. Guemez-Gamboa, A., N. G. Coufal and J. G. Gleeson (2014). 'Primary cilia in the developing and mature brain.' Neuron 82(3): 511-521. Hirokawa, N., Y. Tanaka and Y. Okada (2009). 'Left-right determination: involvement of molecular motor KIF3, cilia, and nodal flow.' Cold Spring Harb Perspect Biol 1(1): a000802. Hong, E. and R. Brewster (2006). 'N-cadherin is required for the polarized cell behaviors that drive neurulation in the zebrafish.' Development 133(19): 3895-3905. Houben, A. J. and W. H. Moolenaar (2011). 'Autotaxin and LPA receptor signaling in cancer.' Cancer Metastasis Rev 30(3-4): 557-565. Joyner, A. L., A. Liu and S. Millet (2000). 'Otx2, Gbx2 and Fgf8 interact to position and maintain a mid-hindbrain organizer.' Curr Opin Cell Biol 12(6): 736-741. Juriloff, D. M. and M. J. Harris (2012). 'A consideration of the evidence that genetic defects in planar cell polarity contribute to the etiology of human neural tube defects.' Birth Defects Res A Clin Mol Teratol 94(10): 824-840. Kiecker, C. and A. Lumsden (2005). 'Compartments and their boundaries in vertebrate brain development.' Nat Rev Neurosci 6(7): 553-564. Kimmel, C. B., W. W. Ballard, S. R. Kimmel, B. Ullmann and T. F. Schilling (1995). 'Stages of embryonic development of the zebrafish.' Dev Dyn 203(3): 253-310. Koike, S., Y. Yutoh, K. Keino-Masu, S. Noji, M. Masu and H. Ohuchi (2011). 'Autotaxin is required for the cranial neural tube closure and establishment of the midbrain-hindbrain boundary during mouse development.' Dev Dyn 240(2): 413-421. Koster, R. W. and S. E. Fraser (2001). 'Direct imaging of in vivo neuronal migration in the developing cerebellum.' Curr Biol 11(23): 1858-1863. Kwan, K. M., E. Fujimoto, C. Grabher, B. D. Mangum, M. E. Hardy, D. S. Campbell, J. M. Parant, H. J. Yost, J. P. Kanki and C. B. Chien (2007). 'The Tol2kit: a multisite gateway-based construction kit for Tol2 transposon transgenesis constructs.' Dev Dyn 236(11): 3088-3099. Lai, S. L., W. L. Yao, K. C. Tsao, A. J. Houben, H. M. Albers, H. Ovaa, W. H. Moolenaar and S. J. Lee (2012). 'Autotaxin/Lpar3 signaling regulates Kupffer's vesicle formation and left-right asymmetry in zebrafish.' Development 139(23): 4439-4448. Lee, S. J., T. H. Chan, T. C. Chen, B. K. Liao, P. P. Hwang and H. Lee (2008). 'LPA1 is essential for lymphatic vessel development in zebrafish.' FASEB J 22(10): 3706-3715. Lowery, L. A. and H. Sive (2004). 'Strategies of vertebrate neurulation and a re-evaluation of teleost neural tube formation.' Mech Dev 121(10): 1189-1197. Lun, M. P., E. S. Monuki and M. K. Lehtinen (2015). 'Development and functions of the choroid plexus-cerebrospinal fluid system.' Nat Rev Neurosci 16(8): 445-457. Marchler-Bauer, A., M. K. Derbyshire, N. R. Gonzales, S. Lu, F. Chitsaz, L. Y. Geer, R. C. Geer, J. He, M. Gwadz, D. I. Hurwitz, C. J. Lanczycki, F. Lu, G. H. Marchler, J. S. Song, N. Thanki, Z. Wang, R. A. Yamashita, D. Zhang, C. Zheng and S. H. Bryant (2015). 'CDD: NCBI's conserved domain database.' Nucleic Acids Res 43(Database issue): D222-226. Mitchell, L. E. (2005). 'Epidemiology of neural tube defects.' Am J Med Genet C Semin Med Genet 135C(1): 88-94. Nishimura, T., H. Honda and M. Takeichi (2012). 'Planar cell polarity links axes of spatial dynamics in neural-tube closure.' Cell 149(5): 1084-1097. Nishioka, T., N. Arima, K. Kano, K. Hama, E. Itai, H. Yukiura, R. Kise, A. Inoue, S. H. Kim, L. Solnica-Krezel, W. H. Moolenaar, J. Chun and J. Aoki (2016). 'ATX-LPA1 axis contributes to proliferation of chondrocytes by regulating fibronectin assembly leading to proper cartilage formation.' Sci Rep 6: 23433. Offermanns, S., V. Mancino, J. P. Revel and M. I. Simon (1997). 'Vascular system defects and impaired cell chemokinesis as a result of Galpha13 deficiency.' Science 275(5299): 533-536. Ohuchi, H., Y. Hayashibara, H. Matsuda, M. Onoi, M. Mitsumori, M. Tanaka, J. Aoki, H. Arai and S. Noji (2007). 'Diversified expression patterns of autotaxin, a gene for phospholipid-generating enzyme during mouse and chicken development.' Dev Dyn 236(4): 1134-1143. Perrakis, A. and W. H. Moolenaar (2014). 'Autotaxin: structure-function and signaling.' J Lipid Res 55(6): 1010-1018. Saunders, L. P., A. Ouellette, R. Bandle, W. C. Chang, H. Zhou, R. N. Misra, E. M. De La Cruz and D. T. Braddock (2008). 'Identification of small-molecule inhibitors of autotaxin that inhibit melanoma cell migration and invasion.' Mol Cancer Ther 7(10): 3352-3362. Stockinger, P., J. L. Maitre and C. P. Heisenberg (2011). 'Defective neuroepithelial cell cohesion affects tangential branchiomotor neuron migration in the zebrafish neural tube.' Development 138(21): 4673-4683. Stracke, M. L., H. C. Krutzsch, E. J. Unsworth, A. Arestad, V. Cioce, E. Schiffmann and L. A. Liotta (1992). 'Identification, purification, and partial sequence analysis of autotaxin, a novel motility-stimulating protein.' J Biol Chem 267(4): 2524-2529. Takacs, C. M. and A. J. Giraldez (2016). 'miR-430 regulates oriented cell division during neural tube development in zebrafish.' Dev Biol 409(2): 442-450. Takeda, S., Y. Yonekawa, Y. Tanaka, Y. Okada, S. Nonaka and N. Hirokawa (1999). 'Left-right asymmetry and kinesin superfamily protein KIF3A: new insights in determination of laterality and mesoderm induction by kif3A-/- mice analysis.' J Cell Biol 145(4): 825-836. Tawk, M., C. Araya, D. A. Lyons, A. M. Reugels, G. C. Girdler, P. R. Bayley, D. R. Hyde, M. Tada and J. D. Clarke (2007). 'A mirror-symmetric cell division that orchestrates neuroepithelial morphogenesis.' Nature 446(7137): 797-800. Thisse, C., B. Thisse, T. F. Schilling and J. H. Postlethwait (1993). 'Structure of the zebrafish snail1 gene and its expression in wild-type, spadetail and no tail mutant embryos.' Development 119(4): 1203-1215. van Meeteren, L. A. and W. H. Moolenaar (2007). 'Regulation and biological activities of the autotaxin-LPA axis.' Prog Lipid Res 46(2): 145-160. van Meeteren, L. A., P. Ruurs, C. Stortelers, P. Bouwman, M. A. van Rooijen, J. P. Pradere, T. R. Pettit, M. J. Wakelam, J. S. Saulnier-Blache, C. L. Mummery, W. H. Moolenaar and J. Jonkers (2006). 'Autotaxin, a secreted lysophospholipase D, is essential for blood vessel formation during development.' Mol Cell Biol 26(13): 5015-5022. Wheeler, N. A., J. A. Lister and B. Fuss (2015). 'The Autotaxin-Lysophosphatidic Acid Axis Modulates Histone Acetylation and Gene Expression during Oligodendrocyte Differentiation.' J Neurosci 35(32): 11399-11414. Wilson, N. H. and E. T. Stoeckli (2012). 'Sonic Hedgehog regulates Wnt activity during neural circuit formation.' Vitam Horm 88: 173-209. Yamaguchi, Y. and M. Miura (2013). 'How to form and close the brain: insight into the mechanism of cranial neural tube closure in mammals.' Cell Mol Life Sci 70(17): 3171-3186. Yuelling, L. W., C. T. Waggener, F. S. Afshari, J. A. Lister and B. Fuss (2012). 'Autotaxin/ENPP2 regulates oligodendrocyte differentiation in vivo in the developing zebrafish hindbrain.' Glia 60(10): 1605-1618. Yukiura, H., K. Hama, K. Nakanaga, M. Tanaka, Y. Asaoka, S. Okudaira, N. Arima, A. Inoue, T. Hashimoto, H. Arai, A. Kawahara, H. Nishina and J. Aoki (2011). 'Autotaxin regulates vascular development via multiple lysophosphatidic acid (LPA) receptors in zebrafish.' J Biol Chem 286(51): 43972-43983. Yung, Y. C., N. C. Stoddard, H. Mirendil and J. Chun (2015). 'Lysophosphatidic Acid signaling in the nervous system.' Neuron 85(4): 669-682. Zhai, G., Q. Gu, J. He, Q. Lou, X. Chen, X. Jin, E. Bi and Z. Yin (2014). 'Sept6 is required for ciliogenesis in Kupffer's vesicle, the pronephros, and the neural tube during early embryonic development.' Mol Cell Biol 34(7): 1310-1321. Goody, M. F. and Henry, C. A. (2013). Phalloidin Staining and Immunohistochemistry of Zebrafish Embryos. Bio-protocol 3(11): e786. http://www.bio- protocol.org/e786 | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/60089 | - |
dc.description.abstract | 自分泌運動因子(Autotaxin, Atx)是一種存在於大部分的體液當中之分泌性溶血磷脂酶,在胚胎發育的過程中扮演著重要的角色。已知在老鼠胚胎中剔除Atx導致血管與神經系統發育不全,並於胚胎發育期9.5天時死亡。然而,Atx調控神經系統發育的作用機制則尚未明瞭,主要由於哺乳動物胚胎發育不易觀察,因此我嘗試利用體外發育且通體透明之斑馬魚建立Atx缺失模式以利機轉探討。首先我觀察到atx表現於出生後11小時至36小時的斑馬魚胚之神經系統,利用嗎 | zh_TW |
dc.description.abstract | Autotaxin (Atx), a secreted form of lysophospholipase D, is present in most body fluids. Atx is vital for different developmental processes. Loss of Atx causes vascular and neural defects and embryonic lethality at E9.5 in mice. However, how Atx affects neural development in vivo remains largely unclear mainly due to the difficulty of studying early development in mammals. To address this point, I used translucent zebrafish embryos as a model. I observed that atx expressed in neural tissues from 11 to 36 hours post fertilization (hpf), corresponding to the period of early neural development. Knockdown of atx with morpholino caused defects in formation of midbrain-hindbrain boundary (MHB) and brain ventricles in 24-hpf embryos. I further examined midbrains of atx morphants by cryosection at various developmental stages and observed that neural tube and midline formation were severely affected. It suggests that the Atx loss of function cause defects in neural cell migration toward the midline during neurulation. Knockdown of atx by MO is known to inhibit the formation of Küpffer vesicle, left-right asymmetry and subsequent cardiac looping in zebrafish. These defects may secondarily interfere with neurulation. To avoid this complication, I generated a neural-specific CRISPR construct targeting atx driven by a gfap promoter and successfully disrupted atx in neural tissue. I found brain and neural tube defects similar to atx morphants in the 48 hpf F0 embryos-injected with the atx-targeting plasmid. The formation of neural tube midline was severely delayed or malformed. Taken together, I unequivocally demonstrate the specific role of Atx in the neural tube formation and brain development in zebrafish. Further investigation using this model could help to elucidate the regulation of neural tube formation by Atx. | en |
dc.description.provenance | Made available in DSpace on 2021-06-16T09:55:21Z (GMT). No. of bitstreams: 1 ntu-105-R03b21006-1.pdf: 3092038 bytes, checksum: b968f55d0af1f467f26430e147d01898 (MD5) Previous issue date: 2016 | en |
dc.description.tableofcontents | Content
口試委員審定書 II 致謝 III 摘要 VI Abstract VII Introduction 1 Materials and methods 5 Zebrafish 5 Morpholino oligonucleotides (MO) 5 Whole-mount in situ hybridization 5 Fluorescence-activated cell sorting (FACS) 6 Genomic DNA extraction 6 Mutation screening 7 RT-PCR 7 Tissue-specific atx disruption in zebrafish embryo 8 Phalloidin staining and cryoscetion 9 Results 10 Sequence characterization of zebrafish autotaxin 10 Expression pattern of atx in zebrafish embryo 11 Knockdown of Atx affects neural development in zebrafish embryo 11 Tissue-specific disrupt atx in developing neural tissue in zebrafish 14 Discussion 17 References 23 Figures 28 Figure 1. Zebrafish Enpp2 (autotaxin) is clustered with human and mouse Enpp2 within Enpp family. 29 Figure 2. Autotaxins (Atx) are highly conserved among human, mouse and zebrafish. 31 Figure 3. The functional domains of autotaxin is conserved in zebrafish as compared to that of human. 33 Figure 4. Expression patterns of autotaxin (atx) in zebrafish embryos fom 11 to 36 hours post fertilization (hpf). 35 Figure 5. Knockdown of atx malformation of brain and midbrain-hindbrain boundary. 37 Figure 6. Knockdown of atx results in neurulation defects. 39 Figure 7. atx CRISPR design. 41 Figure 8. Knockout of atx in gfap expressing cells. 43 Figure 9. Neural tissue specific atx disruption resulted in brain and midline formation defects. 45 Appendix 46 Figure S1. atx MO effenicency (Lai, Yao et al. 2012). 47 | |
dc.language.iso | en | |
dc.title | 組織專一性破壞自分泌運動因子(Autotaxin)導致斑馬魚神經管發育異常 | zh_TW |
dc.title | Tissue-specific Autotaxin Disruption Impairs Neurulation in Zebrafish | en |
dc.type | Thesis | |
dc.date.schoolyear | 105-1 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 管永恕,鄭邑荃,蔡素宜,郭典翰 | |
dc.subject.keyword | 斑馬魚,自分泌運動因子,神經管發育,神經中間線形成, | zh_TW |
dc.subject.keyword | Autotaxin,midline formation,neural tube formation,neurulation,zebrafish, | en |
dc.relation.page | 47 | |
dc.identifier.doi | 10.6342/NTU201603861 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2016-12-30 | |
dc.contributor.author-college | 生命科學院 | zh_TW |
dc.contributor.author-dept | 生命科學系 | zh_TW |
顯示於系所單位: | 生命科學系 |
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