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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 鍾國芳(Kuo-Fang Chung),陳凱儀(Kai-Yi Chen) | |
| dc.contributor.author | Yu-Lan Huang | en |
| dc.contributor.author | 黃郁嵐 | zh_TW |
| dc.date.accessioned | 2021-06-16T09:39:55Z | - |
| dc.date.available | 2020-02-16 | |
| dc.date.copyright | 2017-02-16 | |
| dc.date.issued | 2017 | |
| dc.date.submitted | 2017-02-08 | |
| dc.identifier.citation | 王玉婷 (2012) 島嶼中的島嶼: 從粒線體 DNA 探討臺灣不同高山地區臺灣高山田鼠 (Microtus kikuchii) 的遺傳結構. 東海大學生命科學系碩士論文.
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/59825 | - |
| dc.description.abstract | 臺灣的瓦氏組小檗 (Berberis sect. Wallchianae) 生長於中高海拔山區，Yu & Chung (2014: Phytotaxa 184: 61–99) 人依其外觀形態的差異分為11個種類。大部分的種類有明顯的地理區隔，僅有少部分為同域分布；使用葉綠體片段重建分子親緣關係，結果支持臺灣產所有種類皆為特有種。但由於快速輻射演化而導致DNA累積變異不足，種間關係並無解析度。在本研究取樣所有臺灣瓦氏組小檗種類 (花蓮小檗、長葉小檗、高山小檗、清水山小檗、早田氏小檗、臺灣小檗、眠月小檗、南投小檗、南臺灣小檗、神武小檗和太魯閣小檗) 、菲律賓小檗，以中國大陸產的小檗作為外群，首次使用限制酶位點標定之核酸定序法 (RAD-Seq) 得到大量資料，從中篩選出32,505個單核苷酸多態性 (SNPs) 重建高支持度的分子親緣關係樹。結果顯示臺灣的所有種類可分為三大譜系，並能各自對應特殊外觀形態和地理分布。除了台灣小檗和南臺灣小檗的關係有待釐清以外，分子親緣關係結果皆支持Yu & Chung (2014) 以外觀形態所定義的種，並且支持高地小檗和B. morii sp. nov.為新種。結合末次冰盛期的物種分布模擬和染色體觀察結果 (無多倍體化現象，皆為2n=28)，推論臺灣瓦氏組小檗的種化機制，主要受地理區隔影響。此結果亦證實RAD-Seq的資料，有助於重建快速分化譜系的分子親緣關係。 | zh_TW |
| dc.description.abstract | Berberis sect. Wallichianae in Taiwan can be found in subalpine and montane-temperate areas. Most of them are morphologically and ecologically different, only few are sympatry. Previous phylogenetic study (2014: Phytotaxa 184: 61–99) based on three chloroplast DNA sequences region strongly support them, 11 species, as a monophyletic group endemic to Taiwan, but the phylogenetic relationship among them in the radiation are unresolved. Here we sample all of Taiwanese endemic species (i.e., B. aristatoserrulata, B. brevisepala, B. chingshuiensis, B. hayatana, B. kawakamii, B. mingetsensis, B. nantoensis, B. pengii, B. ravenii, B. schaaliae, and B. tarokoensis) and endemic species from Philippines and China, as the out groups. We constructed a well-supported phylogenetic tree based on 32,505 SNPs filtered from RAD-Seq data. The lineage of three group corresponding to geographic and morphological data. Except for the argument between B. kawakmii and B. pengii, the phylogenetic tree confirmed the delimitations of previous species and two new species (B. alpicola and B. morii sp. nov.). With the results of species niche modeling (Last glacial period) and chromosome number (2n=28), we infer to main speciation by geographic isolation. The result also confirms the utility of RAD-Seq data for reconstructing phylogenetic relationships in young and rapid lineages. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-16T09:39:55Z (GMT). No. of bitstreams: 1 ntu-106-R03625011-1.pdf: 4408479 bytes, checksum: a0e42679191ca19728d4e0e3a8ef1b77 (MD5) Previous issue date: 2017 | en |
| dc.description.tableofcontents | 摘要 i
Abstract ii 目 錄 iii 第一章 前言 1 1-1 臺灣產瓦氏組小檗屬介紹 1 1-2 小檗屬染色體 3 1-3 輻射演化 4 1-4 物種分布模型 6 1-5 限制酶切位相關DNA定序 7 第二章 研究目標 9 第三章 臺灣瓦氏組小檗屬染色體 10 3-1植物材料 10 3-2染色體數量鏡檢觀察 10 3-3染色體數量結果 11 第四章 物種分布模型 13 4-1 物種分布點資料與環境預測圖層 13 4-2 預測分布結果 15 第五章 RAD-Seq 17 5-1 材料與方法 17 5-2 結果 24 第六章 討論 36 6-1 染色體數量與種化機制 36 6-2 物種分布模型 40 6-3 RAD-Seq資料的影響因子 41 6-4 物種界限 43 6-5 臺灣瓦氏組小檗的種化機制 47 第七章 結論 51 第八章 引用文獻 52 | |
| dc.language.iso | zh-TW | |
| dc.title | 使用限制酶位點標定之核酸定序法探討臺灣瓦氏組小檗之輻射演化 | zh_TW |
| dc.title | Resolving evolutionary radiation in Berberis sect.
Wallichianae of Taiwan using restriction site-associated DNA sequencing (RAD-Seq) | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 105-1 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 王震哲(Jenn-Che Wang) | |
| dc.subject.keyword | 輻射演化,臺灣,小檗屬,限制?位點標定之核酸定序法, | zh_TW |
| dc.subject.keyword | Rapid radiation,Berberis,Taiwan,RAD-Seq, | en |
| dc.relation.page | 76 | |
| dc.identifier.doi | 10.6342/NTU201700390 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2017-02-08 | |
| dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
| dc.contributor.author-dept | 森林環境暨資源學研究所 | zh_TW |
| 顯示於系所單位： | 森林環境暨資源學系 | |
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