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???org.dspace.app.webui.jsptag.ItemTag.dcfield??? | Value | Language |
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dc.contributor.advisor | 黃念祖(Nien-Tsu Huang) | |
dc.contributor.author | Jui Chang | en |
dc.contributor.author | 張瑞 | zh_TW |
dc.date.accessioned | 2021-06-16T09:36:39Z | - |
dc.date.available | 2021-09-01 | |
dc.date.copyright | 2020-09-16 | |
dc.date.issued | 2020 | |
dc.date.submitted | 2020-08-17 | |
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dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/59761 | - |
dc.description.abstract | 全血分析提供許多判斷人體健康狀況的參數,如各類血球數目、特定蛋白質濃度等,因此可以提供適當的治療方法。然而,傳統上通常需要訓練有素的醫學檢查人員,大型的量測儀器以及許多冗長的樣本處理過程來從全血樣本中提取血漿來進行血液分析。為了解決這些問題,我們希望研發一個整合比色分析的試紙與無須動力之真空式微流道系統進行微升體積的全血處理和分析。該系統共分為三個區域:一、血漿分離區域,二、檢測區域,三、吸入腔。對於血漿分離,我們設計了一個用於紅細胞沉降的三角形溝槽;同時,我們在裝置中加入了紅血球凝集因子(Anti-D),以加快紅細胞沉降速率(erythrocyte sedimentation rate, ESR)。對於大多數臨床樣品,此晶片可在7分鐘內將未經過任何血液樣本處理步驟的50微升全血樣本中提取10微升的血漿。在血球沉降及血漿純化的過程中,紅血球的去除率約為99.99%,且未觀察到溶血作用。經由血液處理後的血漿將會自動流向比色試紙所在的檢測區域。本論文使用兩種比色試紙,分別為單純葡萄糖試紙檢測及整合奈米金粒之橫向流動免疫測定(Lateral flow immunoassay, LFIA)試紙進行C-reactive protein (CRP) 濃度檢測。上述測試皆可於20分鐘內完成,由於檢測的快速性和系統的便攜性,我們認為此設備具有發展成為定點照護(POC)系統的潛力。 | zh_TW |
dc.description.abstract | Whole blood analysis is one of the most standard methods to understand or monitor one’s health conditions. To achieve a precise whole blood analysis results, it requires well-trained medical examiners, bulky instruments, and multiple sample pretreatment processes to extract blood plasma for blood analysis. To address these problems, we propose a vacuum-driven power-free microfluidic device integrated colorimetric test strip integrated for whole blood processing and analysis. The system consists of three regions: (1) the plasma separation region, (2) the detection region, and (3) the suction chamber. For plasma separation, we design a triangular trench for red blood cells (RBCs) sedimentation. Also, RBCs agglutination reagent was deposited on the trench to accelerate the erythrocyte sedimentation rate (ESR). For most clinical samples, our device can extract about 10 µL plasma from 50 μL whole blood without any sample pretreatment in 7 minutes. The RBCs removal rate is about 99.99% and no hemolysis effect is observed during the process. As for colorimetric detection, the extracted blood plasma automatically flows toward the detection region where a colorimetric-based test strip is located. In this thesis, we use two colorimetric test strips for demonstration, which are glucose colorimetric test strips for blood glucose measurement and gold nanoparticles integrated lateral flow immunoassay (LFIA) test strips for C-reactive protein (CRP) detection. All tests can be done within 20 minutes. Based on the above features, our device shows great potential to be applied in point-of-care (POC) applications. | en |
dc.description.provenance | Made available in DSpace on 2021-06-16T09:36:39Z (GMT). No. of bitstreams: 1 U0001-1308202017350900.pdf: 4837444 bytes, checksum: 350ff15fa4e43bb6618a49dd50cc1f7d (MD5) Previous issue date: 2020 | en |
dc.description.tableofcontents | 口試委員會審定書 # 中文摘要 i ABSTRACT ii CONTENTS iii LIST OF FIGURES vi LIST OF TABLES x Chapter 1 Introduction 1 1.1 Research Background and Motivation 1 1.2 Literature Review 3 1.2.1 Microfluidics for Whole Blood Process 3 1.2.2 Power-free Microfluidics for Plasma Separation 7 1.2.3 Colorimetric Biosensing 13 Chapter 2 Theory 17 2.1 Blood Cell Separation Mechanism 17 2.2 Vacuum Microfluidic Device 18 2.3 Colorimetric Sensing Mechanism 23 2.3.1 Enzymatic Colorimetric Test Strip 23 2.3.2 LFIA Test 24 Chapter 3 Materials and Methods 26 3.1 Whole Blood 26 3.1.1 Blood Sample 26 3.1.2 Defined Parameters to Evaluate Whole Blood Processing Performance 26 3.2 PDMS Microfluidic Device 26 3.2.1 System Overview 26 3.2.2 Device Design 27 3.2.3 PMMA Mold Fabrication 29 3.2.4 PDMS Chip Assembling 30 3.2.5 Vacuum Device Flow Rate Performance and Control 31 3.3 COMSOL Simulation 32 3.4 Colorimetric Test Strip 33 3.4.1 Glucose Test Strip 33 3.4.2 LFIA Test Strip 34 3.5 Experiment Protocol 38 3.5.1 PDMS Device operated by the syringe pump 38 3.5.2 Vacuum Device 39 Chapter 4 Results and Discussion 42 4.1 COMSOL Simulation 42 4.1.1 Trench Length 42 4.1.2 Trench Depth 43 4.1.3 Steps-like Trench 44 4.2 Trench Optimization and Validation 45 4.2.1 Trench Geometry Optimization 45 4.2.2 Dimensionless Validation 46 4.3 Vacuum Microfluidics Device 48 4.3.1 Flow Rate Control 48 4.3.2 Separation Performance 49 4.4 Colorimetric Glucose Analysis 51 4.4.1 Calibration Curve 51 4.4.2 Clinical Sample Analysis 52 4.5 LFIA Test for CRP 54 4.5.1 Off-chip LFIA CRP Measurement 54 4.5.2 Vacuum Device LFIA CRP Measurement 54 Chapter 5 Conclusion 56 Chapter 6 Future Work 57 References 59 | |
dc.language.iso | en | |
dc.title | 無動力式微流道晶片進行全血血漿分離及原位比色法生物標記物檢測
| zh_TW |
dc.title | A Power-free Microfluidic Device for Whole Blood Plasma Separation and In-situ Colorimetric-based Biomarkers Detection | en |
dc.type | Thesis | |
dc.date.schoolyear | 108-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 盧彥文(Yen-Wen Lu),陳建甫(Chien-Fu Chen),楊東霖(Tony Yang) | |
dc.subject.keyword | 全血處理,無動力式微流道,原位檢測,比色檢測, | zh_TW |
dc.subject.keyword | whole blood processing,power-free microfluidics,in-situ detection,colorimetric detection, | en |
dc.relation.page | 63 | |
dc.identifier.doi | 10.6342/NTU202003303 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2020-08-18 | |
dc.contributor.author-college | 電機資訊學院 | zh_TW |
dc.contributor.author-dept | 生醫電子與資訊學研究所 | zh_TW |
Appears in Collections: | 生醫電子與資訊學研究所 |
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U0001-1308202017350900.pdf Restricted Access | 4.72 MB | Adobe PDF |
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