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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 陳惠文(Huei-Wen Chen) | |
dc.contributor.author | Hsin-Tung Liu | en |
dc.contributor.author | 劉芯彤 | zh_TW |
dc.date.accessioned | 2021-06-16T09:31:48Z | - |
dc.date.available | 2022-02-24 | |
dc.date.copyright | 2017-02-24 | |
dc.date.issued | 2017 | |
dc.date.submitted | 2017-02-15 | |
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dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/59656 | - |
dc.description.abstract | 液相層析串聯質譜儀分析廣泛地被應用於諸多領域,在法醫學或生物領域中,奈米級液相層析質譜分析技術相對傳統液相層析又有高靈敏及樣品量需求少等優點,本篇研究欲將其應用於法醫毒理以偵測66種常見濫用藥物、其相關代謝物以及海洛因不純物-acetylthebaine之一級、二級代謝物。然而,分析物含括極性及非極性特性,難以同時使用一種層析管柱進行偵測,因此,本篇研究欲混和兩種固定相於一體以改善此缺點。首先,篩選七種固定相並以Hypersil gold C18及Porous graphite carbon (PGC) 作為最終兩混和材質,接著,嘗試以不同比例及混和方式比較分析物峰形及峰寬,並選擇1P9HG混和型層析管柱有較好之表現。然而,由於PGC的特殊性,一些分析物於最佳化時無法被偵測,推測可能因其有活性碳相似之性質導致分析物吸附,嘗試調整沖提液溫度及游離源電壓,同時評估分析物出現數目,最終發現仍有兩藥物消失(JWH-018 及acetylthebaol) 。排除此現象,整體而言1P9HG混和型層析管柱有最好之結果,期望未來能針對量少且極性程度差異大的樣品有實務上的應用。 | zh_TW |
dc.description.abstract | Liquid chromatography coupling to mass spectrometry is widely used in several areas. In the forensic science or biological area, it is sometimes quite challenging for the detection of compounds in limited amount samples. The miniaturized Nano-LC present as a great choice for compensating this issue. Sixty-six drugs and phase I, II metabolites of acetylthebaine from heroin impurities were selected as our target analytes. A wide range of polarities are included which is quite difficult in detection within one sample shot due to the analytical column limited selection properties. In order to solve the problem, we tried to combine two materials with different properties out of seven columns. Hypersil gold C18 and Porous graphite carbon (PGC) were selected as our final mixing candidates. Several parameters had been evaluated, including mixing ratios and mixing fashions, by comparing analytes’ peak shape and peak width. The final pre-mixing 1P9HG column was selected with good performance to most compounds except for the disappearance for two compounds. Temperature and ion source voltage modulation had been tested, however still, missing in two analytes (JWH-018 and acetylthebaol). Besides the phenomenon, it may be useful in detection of a wide range polarities samples in limited amount. | en |
dc.description.provenance | Made available in DSpace on 2021-06-16T09:31:48Z (GMT). No. of bitstreams: 1 ntu-106-R03447005-1.pdf: 5530016 bytes, checksum: 028beef335a78e2b9faba0748abfa587 (MD5) Previous issue date: 2017 | en |
dc.description.tableofcontents | 誌謝 II
中文摘要 III Abstract IV Contents V List of figures VII List of Tables IX Chapter 1 Introduction 1 1.1 The application of liquid chromatography-mass spectrometry (LC-MS) 1 1.2 LC-MS introduction 3 1.2.1 Mass spectrometry 3 1.2.2 Ionization source – Electrospray ionization (ESI) 3 1.2.3 Mass analyzer-Tandem Mass Spectrometry (MS/MS) with Triple Quadrupole Mass Spectrometer 6 1.2.4 Liquid chromatography 11 1.3 Nanoscale liquid chromatography (Nano-LC) 13 1.4 Introduction of Nano-LC 14 1.5 Social problems caused by drug abuse 16 1.5.1 Heroin abuse 17 1.6 Aims, hypothesis and objectives 23 1.6.1 Aims 23 1.6.2 Hypothesis 23 1.6.3 Objectives 26 Chapter 2 Materials and Methods 27 2.1 Equipment 27 2.2 Materials 27 2.3 Chemicals and reagents 28 2.4 In vitro synthesis of phase I and phase II metabolites 30 2.4.1 Conduction of Phase I reaction 30 2.4.2 Conduction of glucuronide-conjugation 30 2.4.3 Conduction of sulfonate-conjugation 31 2.5 Emitter tip construction 31 2.6 Packing analytical column 31 2.7 Nano-liquid chromatography-Mass spectrometry (Nano-LC-MS) 34 2.8 Data acquisition 35 2.9 Stocking solution and working solution preparation 35 2.10 Column selection 35 2.11 Combination of both polar and nonpolar materials 37 2.12 Conduction of the pre-mixing column 37 2.13 Conduction of the reverse-packing column 37 2.14 Temperature control 38 2.15 Test for ion source voltage 38 Chapter 3 Result and Discussion 39 3.1 In vitro synthesis of phase I and phase II metabolites 39 3.1.1 Phase I reaction 39 3.1.2 Phase II reaction 40 3.2 Column selection 43 3.2.1 Properties of seven candidate columns 43 3.2.2 Column selection 44 3.3 Detection of heroin biomarkers in PGC column 60 3.4 Combination of both polar and nonpolar materials 61 3.5 Conduction of the pre-mixing column 65 3.6 Conduction of the reverse-packing column 67 3.7 Temperature control 70 3.8 Test for different ion source voltage 73 3.9 Retention effect between PGC and target analytes 76 3.10 The disappearance of analytes 78 3.11 Column validation 81 3.12 Calibration curve 84 Appendix 86 References 127 | |
dc.language.iso | en | |
dc.title | 利用雙材料混和型層析管柱串聯奈米級液相層析質譜儀同時偵測極性與非極性濫用藥物 | zh_TW |
dc.title | Improved simultaneous detection of polar and nonpolar drugs by bimaterial hybridized column on nanoLC-MS/MS system | en |
dc.type | Thesis | |
dc.date.schoolyear | 105-1 | |
dc.description.degree | 碩士 | |
dc.contributor.coadvisor | 陳珮珊(Pai-Shan Chen) | |
dc.contributor.oralexamcommittee | 黃賢達,陳家揚 | |
dc.subject.keyword | 奈米級液相層析串聯質譜分析,Hypersil gold C18 層析管柱,PGC層析管柱,濫用藥物,雙材料混和型層析管柱,法醫毒理學, | zh_TW |
dc.subject.keyword | Nano liquid chromatography-tandem mass spectrometry,hypersil gold C18,porous graphite carbon (PGC),illicit drugs,bimaterial hybridized column,forensic toxicology, | en |
dc.relation.page | 128 | |
dc.identifier.doi | 10.6342/NTU201700506 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2017-02-15 | |
dc.contributor.author-college | 醫學院 | zh_TW |
dc.contributor.author-dept | 毒理學研究所 | zh_TW |
顯示於系所單位: | 毒理學研究所 |
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