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http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/59431完整後設資料紀錄
| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 黃義侑(Yi-You Huang) | |
| dc.contributor.author | Meng-Yow Hsieh | en |
| dc.contributor.author | 謝孟佑 | zh_TW |
| dc.date.accessioned | 2021-06-16T09:23:32Z | - |
| dc.date.available | 2027-12-31 | |
| dc.date.copyright | 2017-08-25 | |
| dc.date.issued | 2017 | |
| dc.date.submitted | 2017-06-22 | |
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/59431 | - |
| dc.description.abstract | 溫感性水膠為一種可注射的流體，使用微創手術的方式，可將水膠注入體內至所需的組織或器官進行治療。此外，溫感性水膠具有許多優點，例如藉由溫度的改變，不需要有機溶劑的參予，可將水膠由固態改變成液態，並且，形成原位凝膠狀態。於藥物傳輸和生物醫學的應用面，溫感性水膠藉由水膠型態的轉變，可做為藥物、生物活性因子的優良載體，並應用於組織工程的研究。
論文的研究主題是針對水膠進行骨組織修復進行討論。第一部分研究的主題是使用骨頭生長因子(BMP-2)與水膠進行結合，用於治療股骨頭缺血性壞死（AVN）。由大鼠的動物實驗結果得知，水膠中BMP-2(1μg/ mL)的劑量治療4週後，可有效降低股骨頭缺血性壞死引起的股骨損傷，治療8週後，股骨頭和股骨下生長板下方更多的骨小梁的生成。由上述結果可以得知，水膠混摻BMP-2生長因子的系統，對於股骨頭缺血性壞死的病症的具有不錯的修復潛力。 另一項研究是使用溫感性水膠系統混摻BMP-2生長因子，用於治療骨缺損。藉由相同濃度的BMP-2於不同濃度水膠系統釋放曲線決定出25wt%為最合適的水膠濃度。在動物實驗中，用25wt%的水膠混摻BMP-2進行骨缺損的治療，發現水膠含生長因子的系統，於12週的實驗結果發現骨缺損的部位已有顯著的骨癒合效果。若在12周治療過程中發現，將BMP-2的濃度提高會有更好的骨癒合效果外，若BMP-2的濃度達到20μg/mL時，由臨床的實驗結果得知，骨癒合效果與自體移植組相當。 | zh_TW |
| dc.description.abstract | Thermo-sensitive hydrogels are injectable fluids that can be introduced into the body in a minimally invasive manner prior to solidifying or gelling with the desired tissue or organ. Additionally, the thermos sensitive hydrogels own many advantages, such as it does not require organic solvents and can be an in-situ forming gel. Because of the simplicity of pharmaceutical and biomedical uses of the water-based sol-gel transition that can be used as drug delivery-control systems, bioactive compounds delivery, and tissue engineering. In this study we aimed the bone tissue repair.
The first part was focused on avascular necrosis (AVN) treat with thermogel blend with BMP-2.From results showed that AVN surgery plus MP treatment has successfully reduced the damage of femoral head 4 weeks after treatment. A dosage of BMP-2 (1μg/mL) in hydrogel has effectively reduced the femoral damage induced by AVN surgery plus MP treatment by preserving more epiphysis of the femoral head and more trabeculae below the growth plate of the femoral head 8 weeks after treatment. Furthermore, BMP-2 in hydrogel attenuated the femoral head damage after AVN surgery plus MP treatment at doses of 1 and 2μg/mL. These findings strongly suggest that BMP-2 in the hydrogel has a great potential for treating patients suffering from avascular necrosis or other conditions characterized by the osteonecrosis of bones. The other study was to develop a suitable biodegradable thermosensitive hydrogel system as a carrier for bone morphogenetic protein (BMP)-2 delivery in the treatment of critical-sized femoral defects. The BMP-2 release from BOX hydrogel exhibited a near-linear release profile in vitro. In animal experiments, treatment of critical-sized bony defects with 25 wt% BOX hydrogel carrying BMP-2 effectively promoted fracture healing during the 12-week trial period and higher concentrations of BMP-2 treatment correlated with better bone quality. Most importantly, clinical outcome and bone healing in the BOX-hydrogel group with 20 μg/mL BMP-2 were nearly equivalent to those in the autograft group in a 12-week treatment course. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-16T09:23:32Z (GMT). No. of bitstreams: 1 ntu-106-D00548001-1.pdf: 3121131 bytes, checksum: 08446cdd48b777c777f526dabc452a41 (MD5) Previous issue date: 2017 | en |
| dc.description.tableofcontents | 誌謝 i
中文摘要 ii ABSTRACT iii CONTENTS v LIST OF FIGURES ix LIST OF TABLES xii Chapter 1 Introduction 1 1.1 Background of the Study 2 1.1.1 Study for Avascular Necrosis 2 1.1.2 Study for Bone Defect 2 1.2 Motivation of the Study 4 1.3 Research Aim 5 Chapter 2 Review of the Literatures 7 2.1 Smart Materials 8 2.1.1 Smart Materials for Electrochromic 8 2.1.2 Smart Materials for Electrostrictive 8 2.1.3 Light-Responsive Materials 9 2.1.4 Smart Materials for Shape Memory 9 2.1.5 Thermoresponsive Materials 10 2.2 Hydrogels 11 2.3 Thermosensitive Hydrogel 14 2.4 Tissue Engineering 16 2.4.1 History of Tissue Engineering 16 2.4.2 In Situ Tissue Engineering 17 2.5 Bone Morphogenetic Proteins 19 2.6 Avascular Necrosis 21 2.7 Bone Defects 22 2.8 Thermogel Composite for Bone Tissue Regeneration 24 Chapter 3 Materials and Method 26 3.1 Materials 27 3.2 Synthesis of BOX Copolymer 28 3.3 Characteristic of BOX Copolymer 29 3.3.1 Gel Permeation Chromatography (GPC) Characterization 29 3.3.2 1H Nuclear Magnetic Resonance Measurements 29 3.3.3 Determination of Sol-Gel Phase Transition By the Test Tube Inverting Method 29 3.3.4 Determination of Sol-Gel-Sol Phase Transition By Rheometer 30 3.4 Biocompatibility Test-Agar Diffusion 31 3.5 Cytotoxicity Assay 33 3.6 Degradation of Hydrogel Copolymers 34 3.7 Release of BMP-2 From the BOX Hydrogel 35 3.8 Treatment of the AVN Animal Model with BOX Hydrogel Carrying BMP-2 36 3.8.1 Rat Model of Avascular Necrosis 36 3.8.2 Statistics Analysis 37 3.9 Treatment of Critically Sized Femoral Defects Animal Model with BOX Hydrogel Carrying BMP-2 38 3.9.1 Intracutaneous Irritation Test 38 3.9.2 Creation of Critically Sized (10 mm) Femoral Defects 38 3.9.3 Radiographic Analysis and μCT 39 3.9.4 Histological Staining 39 3.9.5 Biomechanical Testing 39 3.9.6 Western Blot Analysis 40 3.9.7 Statistical Analysis 40 Chapter 4 Results 41 4.1 Characterization of the BOX Copolymer 42 4.2 Thermosensitive Sol-Gel-Sol Transition of mPEG-PLGA and BOX Copolymers 43 4.3 In Vitro Degradation of mPEG-PLGA and BOX Hydrogels Copolymers 45 4.4 pH Changes in mPEG-PLGA and BOX Hydrogels During Hydrolytic Degradation 46 4.5 Biocompatibility Test for Agar Diffusion 47 4.6 Cytotoxicity of mPEG-PLGA and BOX Hydrogels 48 4.7 In Vitro BMP-2 Release From the BOX Hydrogel 49 4.8 Rat Model of Avascular Necrosis 50 4.9 Treatment of Critical-Sized Bone Defects with the BMP-2 Loaded BOX Hydrogel 51 4.10 Evaluation of Bone Repair By the Examination of Bone Remodeling Markers 52 4.11 Bone Healing Evaluated By Gross Appearance and μCT Analysis 53 4.12 Biomechanical Tests of Bone Healing 54 Chapter 5 Discussion 55 5.1 The Characteristic of Thermogel 56 5.2 The Growth Factor Release (BMP-2) from Thermogel 57 5.3 Treatment of the AVN with Biodegradable Thermosensitive Hydrogel 58 5.4 Repair of Bone Defects with Biodegradable Thermosensitive Hydrogel 59 Chapter 6 Conclusions and Future Work 62 6.1 Thermogel 63 6.2 Treatment of the AVN with Biodegradable Thermosensitive Hydrogel Carrying BMP-2 64 6.3 Treatment of Critically Sized Femoral Defects with Recombinant BMP-2 Delivered by Biodegradable Thermosensitive Hydrogel 65 6.4 Future Work 66 REFERENCES 67 | |
| dc.language.iso | en | |
| dc.subject | 骨缺損 | zh_TW |
| dc.subject | 溫感性水膠 | zh_TW |
| dc.subject | 原位 | zh_TW |
| dc.subject | 骨頭生長因子 | zh_TW |
| dc.subject | 股骨頭缺血性壞死 | zh_TW |
| dc.subject | avascular necrosis | en |
| dc.subject | in-situ | en |
| dc.subject | BMP-2 | en |
| dc.subject | femoral defects | en |
| dc.subject | thermogel | en |
| dc.title | 溫感水膠複合物於骨組織再生之應用 | zh_TW |
| dc.title | Application of Thermogel Composite for Bone Tissue Regeneration | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 105-2 | |
| dc.description.degree | 博士 | |
| dc.contributor.oralexamcommittee | 陳嘉明(Jia-Ming Chern),彭國狄(Kuo-Ti Peng),黃意真(Yi-Cheng Huang),許馨云(Hsin-Yun Hsu) | |
| dc.subject.keyword | 溫感性水膠,原位,骨頭生長因子,股骨頭缺血性壞死,骨缺損, | zh_TW |
| dc.subject.keyword | thermogel,in-situ,BMP-2,avascular necrosis,femoral defects, | en |
| dc.relation.page | 110 | |
| dc.identifier.doi | 10.6342/NTU201700756 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2017-06-22 | |
| dc.contributor.author-college | 工學院 | zh_TW |
| dc.contributor.author-dept | 醫學工程學研究所 | zh_TW |
| 顯示於系所單位： | 醫學工程學研究所 | |
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