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標題: | 探討紅麴二次代謝物monascin和lovastatin對過度糖化終產物誘導的第二型糖尿病小鼠之影響 The effects of monascin and lovastatin on type 2 diabetes in advanced glycation end-product (AGEs) induced mice |
作者: | Si-Shi Lu 呂思詩 |
指導教授: | 潘子明(Tzu-Ming Pan) |
關鍵字: | monascin,過度糖化終產物,胰島素阻抗,抗氧化酵素, monascin,advanced glycation end-products,insulin resistance,antioxidant enzymes, |
出版年 : | 2013 |
學位: | 碩士 |
摘要: | 本研究以過度糖化終產物 (advanced glycation end-products, AGEs) 建立糖尿病小鼠之動物模式,並同時以腹腔注射 monascin (MS) 和lovastatin (LOV),評估MS和LOV能否改善胰島素阻抗和血糖調節機制。
研究結果顯示, AGE誘導能夠降低小鼠之胰島素敏感性和葡萄糖耐受性。MS 和LOV 能藉由提升胰島中胰島素的合成和抗氧化酵素的表現以降低AGE誘導產生之高血糖和胰島素阻抗之症狀。結果顯示AGE可能會導致小鼠胰臟β細胞功能障礙和氧化壓力從而產生胰島素抗性。 本研究發現與控制組相比AGE組有胰島素阻抗之現象,包括血糖上升,血清胰島素含量增加,而血清TG,TC 也分別上升。抗發炎方面,AGE組促發炎細胞激素TNF-α、IL-6和IL-1β表現量顯著提升,而MS和LOV處理後會有抑制促發炎細胞激素表現之效果。抗氧化方面,AGE組肝臟p47phox (NADPH oxidase subunit) 蛋白質表現顯著增加 (p < 0.05),且超氧化物歧化酶 (superoxide dismutase, SOD)、觸酶 (catalase, CAT) 和麩胱甘肽 (glutathione, GSH) 皆顯著較控制組低。同時用MS和LOV處理後,肝臟p47phox蛋白質表現量受到抑制,抗氧化酵素SOD、CAT與GSH亦有上升的趨勢。 The aim of this study is to investigate the improvement of moanscin (MS) and lovastatin (LOV) on insulin resistance and the mechanism of MS and LOV for blood glucose regulation. In this study we also provide evidence that advanced glycation end-products (AGEs) are able to affect insulin sensitivity and glucose tolerance in vivo. AGE also reduced expression of islet insulin. Together these results suggest that the AGEs pathway may play an important role in the loss of functional β-cell mass in diabetes, at least in part through the induction of oxidative stress and the alternation of β-cell differentiation and survival. We found that AGE induction significantly resulted in hyperglycemia in BALB/c mice, but MS and LOV effectively inhibited inflammation and hyperglycemia caused by AGE. MS and LOV inhibited TNF-α, IL-6, IL-1β expression in pancreases, liver and kidney. We also found that MS and LOV inhibited p47phox (NADPH subunit) expression in liver. Results demonstrated that MS and LOV elevated antioxidant enzyme: superoxide dismutase (SOD), catalase (CAT) and glutathione (GSH) in pancreases, liver and kidney. |
URI: | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/5931 |
全文授權: | 同意授權(全球公開) |
顯示於系所單位: | 生化科技學系 |
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