請用此 Handle URI 來引用此文件：
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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 林致廷(Chih-Ting Lin) | |
| dc.contributor.author | Ting-Wei Wu | en |
| dc.contributor.author | 吳亭緯 | zh_TW |
| dc.date.accessioned | 2021-06-16T09:18:05Z | - |
| dc.date.available | 2019-08-01 | |
| dc.date.copyright | 2017-07-17 | |
| dc.date.issued | 2017 | |
| dc.date.submitted | 2017-07-10 | |
| dc.identifier.citation | Chia-Hong Gao and Chih-Ting Lin, “A microfluidic particle-analyzing device with novel coplanar electrode design based on impedance sensing,” Master Thesis, Graduate Institute of Electronics Engineering, July, 2016.
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/59218 | - |
| dc.description.abstract | 流式細胞技術於分子生物學、生理學、檢驗技術上皆扮演著舉足輕重的腳色。而其中雷射光學方式利用分析細胞通過反射或折射的螢光，常用來作為精準的單一細胞分析。但此種方式常受限於龐大的儀器體積、專業人才使用等高成本，常僅在醫療或研究機構做準確細胞篩選檢測。因此電阻抗式流式細胞技術因而被提出，用來作為體積小、操作簡易、成本低的可攜式裝置。本研究即延續已初步設計的搭載新型平面式電極的電阻抗式流式細胞技術，加以討論在交流電訊號下不同頻率對其分析不同粒子特性的影響，找出適當的使用頻寬。並且利用一系列實驗來佐證不同頻率下分析的能力。找出頻寬後，透過基板材質與不同電極設計來拓寬此使用頻寬來達到更佳的儀器使用效果。最後使用新型電極設計來進行一系列的實驗，來驗證本研究的元件，具有在高操作頻率下判斷直徑6和10微米的聚苯乙烯粒子、具-COOH基的該粒子、二氧化矽、三聚氰胺、鐵磁粒子、人急性單核性白血病细胞(THP1)等種類的能力。本研究所改良基板與電極的元件，可同時偵測粒子和細胞大小、位置、種類的資訊，來對初步疾病篩檢目的有重要的參考意義。 | zh_TW |
| dc.description.abstract | Flow cytometry takes up an important role in molecular and cellular biology, physiology and diagnosis techniques. One of flow cytometry method, the laser light system, utilizes analysis on reflection and refraction of fluorescent light from a passing cell. It is always implemented as a precise single cell analysis. But this method is always constrained to high cost such as a huge equipment size and professional use. It could be only practiced in medical or research institution for accurate cell sorting and detection. Therefore, impedance flow cytometry is then proposed to be a small, easy and low-cost portable device. This research then follows the preliminarily designed impedance flow cytometry with new coplanar electrode design. It discusses the influence of device impedance spectroscopy on analyzing different particle properties by finding out an adequate operational frequency range. By experiments, its ability will be demonstrated to analyze under multi-frequencies. When finding out the frequency bandwidth, a better sensing performance of the device will be achieved by considering substrate materials and new electrode spatial designs. At last, the new device with a specific electrode design will be verified by a set of experiments. It demonstrates the ability of differentiating 6μm and 10μm plain polystyrene, carboxylated polystyrene, silica, melamine, magnetic beads and THP1 Cell. The proposed device with modified substrate and electrode design could classify bead and cell’s size, position and type, which has imperative meaning for initial disease diagnosis purpose. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-16T09:18:05Z (GMT). No. of bitstreams: 1 ntu-106-R04943173-1.pdf: 5808050 bytes, checksum: aa097bce00d840dd71912af708c0efb3 (MD5) Previous issue date: 2017 | en |
| dc.description.tableofcontents | Certificate of approval by oral committee i
Acknowledgement ii Abstract in Chinese iii Abstract in English iv Contents vi List of Figures viii Chapter 1. Introduction 1 1.1 Structure of the Thesis 1 1.2 Overview of Bio-material Sensing Technology 2 1.3 Review for Microfluidics Impedance Flow Cytometry 5 1.4 Motivation of My Work 12 Chapter 2. Theory 18 2.1 Coulter Principle 18 2.2 Developed Equivalent Circuit Model 20 2.3 Impedance Multi-Frequencies Spectroscopy 23 (1) Operational Frequency Range 23 (2) Substrate Material Effect 25 (3) Electrode Spatial Design 26 2.4 Theory of Particle Sensing 30 (1) Lock-In Amplifier 30 (2) Particle Sensing Under Multi-frequencies 32 (3) Dielectric Analysis 34 Chapter 3. Experiment 37 3.1 Device Fabrication 37 3.2 Device Design 42 (1) Overall Design 42 (2) Substrate Material Design 47 (3) Electrode Design 50 3.3 Materials and Measurement Parameters 52 3.4 Experiment Method and Setup 54 Chapter 4. Results and Discussion 60 4.1 Operational Frequency Range Simulation and Verification 60 4.2 Substrate Material Effect 67 4.3 Electrode Spatial Design Effect 73 4.4 Bead Surface and Material Effect 84 (1) Surface Effect 84 (2) Material Effect 87 Chapter 5. Conclusions 93 Reference 95 | |
| dc.language.iso | en | |
| dc.title | 一種微流體粒子分析元件的阻抗頻譜發展與應用 | zh_TW |
| dc.title | The development of a microfluidic particle-analyzing device by impedance spectroscopy | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 105-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 陳奕帆(Yih-Fan Chen),李舒昇(Shu-Sheng Lee),劉怡劭(Yi-Shao Liu) | |
| dc.subject.keyword | 單一細胞檢測技術,微流體,流式細胞儀,阻抗頻譜分析,電極設計, | zh_TW |
| dc.subject.keyword | single cell detection,microfluidics,flow cytometry,impedance spectroscopy,electrode design, | en |
| dc.relation.page | 102 | |
| dc.identifier.doi | 10.6342/NTU201701263 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2017-07-11 | |
| dc.contributor.author-college | 電機資訊學院 | zh_TW |
| dc.contributor.author-dept | 電子工程學研究所 | zh_TW |
| 顯示於系所單位： | 電子工程學研究所 | |
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