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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 周崇熙 | |
| dc.contributor.author | Tzu-Sheng Chuang | en |
| dc.contributor.author | 莊子昇 | zh_TW |
| dc.date.accessioned | 2021-05-16T16:18:16Z | - |
| dc.date.available | 2015-08-17 | |
| dc.date.available | 2021-05-16T16:18:16Z | - |
| dc.date.copyright | 2013-08-17 | |
| dc.date.issued | 2013 | |
| dc.date.submitted | 2013-08-15 | |
| dc.identifier.citation | 陳青聖。台灣地區養雞場沙門氏菌之分離與鑑定。碩士論文。國立屏東科技大學獸醫學系。2010。
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/5910 | - |
| dc.description.abstract | 沙門氏菌是重要的人畜共通傳染疾病，在飼養環境中很難被清除，其原因是由於許多無症狀的帶原動物藉由糞便間歇性排菌。又因廣泛使用抗菌劑，造成沙門氏菌的抗藥性增加，目前已出現有抗藥性細菌在人畜之間傳播。細菌抗藥性的產生可能是由於染色體的突變，抑或是抗藥性質體或者轉位子的存在加速抗藥性基因散佈。近幾年來又有新的抗藥性基因移動模式被提出，此模式為integron。本研究於2011-2012年間，蒐集雞隻及其環境來源沙門氏菌共22株，探討菌株是否含有第一型integron及其相關抗藥特性。調查結果發現，一日齡雛雞沙門氏菌均無偵測出integron，而大於一日齡雞隻及環境樣本則能檢出integron，檢出率分別為50 %（3/6）與75 %（6/8）。抗藥性基因方面，具有integron的菌株至少能檢測出三種抗藥性基因，而無integron菌株僅一株能檢測出三種抗藥性基因。一日齡雛雞及大於一日齡雞隻以TEM（57 %, 8/14）檢出率最高；環境樣本以dfrA1（63 %, 5/8）及PSE（63 %, 5/8）檢出率最高。在菌株抗藥性方面，具有Integron菌株至少對五種抗菌劑有抗藥性，一日齡雛雞及大於一日齡雞隻以Ampicillin（86 %, 12/14）抗藥性最高；環境樣本則以Ampicillin、Oxytetracycline及Tetracycline抗藥性最高，佔88 %（7/8）。本研究以統計方式分析出一日齡雛雞的沙門氏菌其多重抗藥性，低於來自飼養環境的沙門氏菌（P<0.05）。可能原因是由於家禽飼養的過程中廣泛使用抗菌劑，使環境中的沙門氏菌抗藥性愈趨嚴重。另外，具integron菌株呈現多重抗藥性的比例遠高於不具integron菌株，顯示integron與多重抗藥性有密切關係。 | zh_TW |
| dc.description.abstract | Salmonella is an important zoonotic pathogen that is difficult to control in food animal environment. However abuses of several drugs for the treatment of Salmonella infection had resulted in increment of antimicrobial resistance. Recently, there has been emergence of antimicrobial resistance in zoonotic diseases. In pathogenic bacteria, their drug resistance may be procurement by mutation or the transfer of plasmid or transposon. In recent year, many studies have demonstrated mobile DNA elements with a specific structure which obtains or exchanges the antibiotic resistance genes these elements that have been termed integrons.Samples for this study were collected from 2011 to 2012 from chicken and environment in Chicken farms and about 22 bacterial strains were isolated. Further studies on understanding the process of antimicrobial resistance and class 1 integron. The results have shown that no class 1 integron was detected in day old chicks. Chickens and environment samples can be detected integron, the detection rate was 50 % (3/6) and 75 % (6/8). The resistance genes: Integron-positive isolates was capable of detecting at least three types of resistance genes, integron-negative isolates only one was able to detect three types of resistance genes. Day-old chicks and chickens were the highest detection rate of TEM (57 %, 8/14). The highest detection rate of environment sample was dfrA1 (63 %, 5/8) and PSE (63 %, 5/8). About antimicrobial resistance, Integron-positive isolates have at least five antimicrobial resistance. Day-old chicks and chickens were the highest detection rate of Ampicillin (86%, 12/14). Ampicillin、oxytetracycline and tetracycline of environment sample get 88 % (7/8) of resistance. The result of statistical analysis is day-old chicks of resistance Salmonella lower than other raising environments (P<0.05). Due to the poultry process, the widespread use of antibacterial agents, increasingly resistant Salmonella. Furthermore, The integron-postive strains showed multi-resistance is much higher than the proportion of integron-negative strains. Which shows integron and multi-resistance have close relatationship | en |
| dc.description.provenance | Made available in DSpace on 2021-05-16T16:18:16Z (GMT). No. of bitstreams: 1 ntu-102-R99629036-1.pdf: 2161472 bytes, checksum: 3bbc240d92e7a29e79c6ce9fd361203a (MD5) Previous issue date: 2013 | en |
| dc.description.tableofcontents | 口試委員會審定書 #
誌謝… i 摘要… iii ABSTRACT iv 目錄… vi 表次… ix 圖次… x 第一章 緒言.. 1 第二章 文獻探討 3 第一節 沙門氏菌簡介 3 2.1.1 沙門氏菌命名與分類 3 2.1.2 沙門氏菌之型態與特性 4 第二節 沙門氏菌分離與鑑定 6 2.2.1 前增菌培養（pre-enrichment） 6 2.2.2 選擇性增菌培養（selective enrichment） 6 2.2.3 選擇性鑑別培養（selective isolation） 7 2.2.4 沙門氏菌之生化學 8 2.2.5 沙門氏菌血清學鑑定 8 第三節 沙門氏菌之流行病學 10 2.3.1 沙門氏菌症 10 2.3.2 人類沙門氏菌之流性病學 10 2.3.3 家禽沙門氏菌症與公共衛生的關係 11 第四節 沙門氏菌之抗藥性問題 14 第五節 沙門氏菌抗藥性基因傳播 17 2.5.1 整合子與基因片匣基本特徵及種類 18 2.5.2 Integron之流行病學調查 19 2.5.3 基因卡匣之流行病學調查 21 第三章 材料與方法 23 第一節 採樣流程與方法 23 3.1.1 檢體處理 23 第二節 沙門氏菌之分離與生化鑑定 25 3.2.1 沙門氏菌之分離培養 25 3.2.2 沙門氏菌之生化鑑定 25 3.2.3 沙門氏菌血清鑑定 26 第三節 沙門氏菌感受性試驗 29 第四節 以聚合酶酵素鏈鎖反應檢測抗藥性基因 30 3.4.1 Genomic DNA 萃取流程 30 3.4.2 抗藥性基因隻之偵測 31 3.4.3 PCR產物電泳 34 3.4.4 基因序列電泳分析 34 第五節 以巢式聚合酶酵素鏈鎖反應檢測抗藥性基因是否存在於integron 35 3.5.1 First round PCR 35 3.5.2 使用Gel/PCR DNA Fragments Extraction kit 35 3.5.3 Second round PCR(nested PCR) 36 第六節 統計方法 37 第四章 結果… 38 第一節 沙門氏菌分離株血清型的分布 39 第二節 沙門氏菌分離株之藥物感受性試驗結果 40 第三節 沙門氏菌分離株之抗藥性基因偵測 42 第五章 討論.. 46 第一節 雞場沙門氏菌之汙染率討論 46 第二節 沙門氏菌之血清型分佈 49 第三節 雞隻及環境分離株之抗藥性 52 第四節 雞隻及環境分離株之抗藥性基因 55 第六章 結論.. 59 第七章 參考文獻 61 Table 1 Reference inhibition zone for antimicrobial resistance test of each tested drug. 72 Table 2 Primer sequences and annealing temperatures used in the PCR reactions. 73 Table 3 Percentage of susceptibility over drugs for Salmonella at chicken farms 74 Table 4 Antimicrobial reisitance of 14 Salmonella from day-old chicks and chickens 75 Table 5 Antimicrobial resistance of 16 Salmonella from day-old chicks and raising environment. 76 Table 6 Antimicrobial resistance of 14 Salmonella from chickens and raising environment. 77 Table 7 Multidrug-resistant Salmonella isolates from day-old chicks and chickens 78 Table 8 Multidrug-resistant Salmonella isolates from day-old chicks and raising environment 78 Table 9 Association between antibiotic susceptibility profile and integrons in 22 isolates 79 Fig 1. PCR amplification of class 1 integrons among different serotypes of Salmonella 80 Fig 2. Detection of aadA gene in 14 Salmonella isolate from chicken 80 Fig 3. Detection of dfrA1 gene in 14 Salmonella isolate from chicken 81 Fig 4. Detection of PSE gene in 14 Salmonella isolate from chicken 81 Fig 5. Detection of Sul gene in 14 Salmonella isolate from chicken 82 Fig 6. Detection of TEM gene in 14 Salmonella isolate from chicken. 82 Fig 7. Detection of tetA gene in 14 Salmonella isolate from chicken 83 Fig 8. Detection of tetB gene in 14 Salmonella isolate from environment. 83 Fig 9. Detection of aadA gene in 8 Salmonella isolate from environment 84 Fig 10. Detection of dfrA1 gene in 8 Salmonella isolate from environment. 84 Fig 11. Detection of PSE gene in 8 Salmonella isolate from environment 85 Fig 12. Detection of Sul gene in 8 Salmonella isolate from environment 85 Fig 13 Detection of TEM gene in 8 Salmonella isolate from environment 86 Fig 14. Detection of tetA gene in 8 Salmonella isolate from environment 86 Fig 15. Detection of tetB gene in 8 Salmonella isolate from environment 87 Fig 16. 2.0 kb cassette array DNA sequence 88 Fig 17. Detection resistance genes from 22 isolates 89 | |
| dc.language.iso | zh-TW | |
| dc.subject | 整合子 | zh_TW |
| dc.subject | 抗藥性 | zh_TW |
| dc.subject | 雞隻 | zh_TW |
| dc.subject | 沙門氏菌 | zh_TW |
| dc.subject | antimicrobial resistance | en |
| dc.subject | integron | en |
| dc.subject | salmonella | en |
| dc.subject | chicken | en |
| dc.title | 雞隻及其飼養環境來源沙門氏菌抗藥特性調查 | zh_TW |
| dc.title | Characterization of Antimicrobial Resistance in Salmonella from Chicken and Its Raising Environment | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 101-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 蔡向榮,張紹光,沈瑞鴻,郭鴻志 | |
| dc.subject.keyword | 抗藥性,雞隻,沙門氏菌,整合子, | zh_TW |
| dc.subject.keyword | antimicrobial resistance,chicken,salmonella,integron, | en |
| dc.relation.page | 89 | |
| dc.rights.note | 同意授權(全球公開) | |
| dc.date.accepted | 2013-08-15 | |
| dc.contributor.author-college | 獸醫專業學院 | zh_TW |
| dc.contributor.author-dept | 獸醫學研究所 | zh_TW |
| 顯示於系所單位： | 獸醫學系 | |
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