請用此 Handle URI 來引用此文件:
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/59052
完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 余家利(Chia-Li Yu) | |
dc.contributor.author | Yu-Chien Tang | en |
dc.contributor.author | 唐語謙 | zh_TW |
dc.date.accessioned | 2021-06-16T08:47:00Z | - |
dc.date.available | 2013-09-24 | |
dc.date.copyright | 2013-09-24 | |
dc.date.issued | 2013 | |
dc.date.submitted | 2013-08-20 | |
dc.identifier.citation | 1. Huang, C.Y., et al., Nationwide surveillance in uterine cancer: survival analysis and the importance of birth cohort: 30-year population-based registry in Taiwan. PLoS One, 2012. 7(12): p. e51372.
2. Boll, D., et al., Incidence and survival trends of uncommon corpus uteri malignancies in the Netherlands, 1989-2008. Int J Gynecol Cancer, 2012. 22(4): p. 599-606. 3. Bokhman, J.V., Two pathogenetic types of endometrial carcinoma. Gynecol Oncol, 1983. 15(1): p. 10-7. 4. Taxt, T., et al., Multispectral analysis of uterine corpus tumors in magnetic resonance imaging. Magn Reson Med, 1992. 23(1): p. 55-76. 5. Prat, J., et al., Endometrial carcinoma: pathology and genetics. Pathology, 2007. 39(1): p. 72-87. 6. Llobet, D., et al., Molecular pathology of endometrial carcinoma: practical aspects from the diagnostic and therapeutic viewpoints. J Clin Pathol, 2009. 62(9): p. 777-85. 7. Creasman, W., Revised FIGO staging for carcinoma of the endometrium. Int J Gynaecol Obstet, 2009. 105(2): p. 109. 8. Gentleman, R.C., et al., Bioconductor: open software development for computational biology and bioinformatics. Genome Biol, 2004. 5(10): p. R80. 9. Bolstad, B.M., et al., A comparison of normalization methods for high density oligonucleotide array data based on variance and bias. Bioinformatics, 2003. 19(2): p. 185-93. 10. Shi, L., et al., The balance of reproducibility, sensitivity, and specificity of lists of differentially expressed genes in microarray studies. BMC Bioinformatics, 2008. 9 Suppl 9: p. S10. 11. Masson, S., et al., Up-regulated expression of HGF in rat liver cells after experimental endotoxemia: a potential pathway for enhancement of liver regeneration. Growth Factors, 2001. 18(4): p. 237-50. 12. Cheng, W.F., et al., High mesothelin correlates with chemoresistance and poor survival in epithelial ovarian carcinoma. Br J Cancer, 2009. 100(7): p. 1144-53. 13. Ludwig, J.A. and J.N. Weinstein, Biomarkers in cancer staging, prognosis and treatment selection. Nat Rev Cancer, 2005. 5(11): p. 845-56. 14. Agrawal, D., et al., Osteopontin identified as lead marker of colon cancer progression, using pooled sample expression profiling. J Natl Cancer Inst, 2002. 94(7): p. 513-21. 15. Kainkaryam, R.M., et al., Smart pooling of mRNA samples for efficient transcript profiling. Methods Mol Biol, 2012. 876: p. 189-94. 16. Ruotsalainen, H., et al., Complete genomic structure of mouse lysyl hydroxylase 2 and lysyl hydroxylase 3/collagen glucosyltransferase. Matrix Biol, 2001. 20(2): p. 137-46. 17. van der Slot, A.J., et al., Identification of PLOD2 as telopeptide lysyl hydroxylase, an important enzyme in fibrosis. J Biol Chem, 2003. 278(42): p. 40967-72. 18. Noda, T., et al., PLOD2 induced under hypoxia is a novel prognostic factor for hepatocellular carcinoma after curative resection. Liver Int, 2012. 32(1): p. 110-8. 19. Dong, S., et al., Histology-based expression profiling yields novel prognostic markers in human glioblastoma. J Neuropathol Exp Neurol, 2005. 64(11): p. 948-55. 20. Chang, H.Y., et al., Gene expression signature of fibroblast serum response predicts human cancer progression: similarities between tumors and wounds. PLoS Biol, 2004. 2(2): p. E7. 21. Arao, T., et al., ZD6474 inhibits tumor growth and intraperitoneal dissemination in a highly metastatic orthotopic gastric cancer model. Int J Cancer, 2006. 118(2): p. 483-9. 22. Baek, J.Y., et al., Characterization of human phosphoserine aminotransferase involved in the phosphorylated pathway of L-serine biosynthesis. Biochem J, 2003. 373(Pt 1): p. 191-200. 23. Ojala, P., et al., mRNA differential display of gene expression in colonic carcinoma. Electrophoresis, 2002. 23(11): p. 1667-76. 24. Friederichs, J., et al., Gene expression profiles of different clinical stages of colorectal carcinoma: toward a molecular genetic understanding of tumor progression. Int J Colorectal Dis, 2005. 20(5): p. 391-402. 25. Vie, N., et al., Overexpression of phosphoserine aminotransferase PSAT1 stimulates cell growth and increases chemoresistance of colon cancer cells. Mol Cancer, 2008. 7: p. 14. 26. Martens, J.W., et al., Association of DNA methylation of phosphoserine aminotransferase with response to endocrine therapy in patients with recurrent breast cancer. Cancer Res, 2005. 65(10): p. 4101-17. 27. Toyama, A., et al., Proteomic characterization of ovarian cancers identifying annexin-A4, phosphoserine aminotransferase, cellular retinoic acid-binding protein 2, and serpin B5 as histology-specific biomarkers. Cancer Sci, 2012. 103(4): p. 747-55. 28. Yasuda, M., et al., Expression of hypoxia inducible factor-1alpha (HIF-1alpha) and glucose transporter-1 (GLUT-1) in ovarian adenocarcinomas: difference in hypoxic status depending on histological character. Oncol Rep, 2008. 19(1): p. 111-6. 29. Behrooz, A. and F. Ismail-Beigi, Dual control of glut1 glucose transporter gene expression by hypoxia and by inhibition of oxidative phosphorylation. J Biol Chem, 1997. 272(9): p. 5555-62. 30. Macheda, M.L., S. Rogers, and J.D. Best, Molecular and cellular regulation of glucose transporter (GLUT) proteins in cancer. J Cell Physiol, 2005. 202(3): p. 654-62. 31. Kim, K., et al., Prognostic Relevance of the Expression of CA IX, GLUT-1, and VEGF in Ovarian Epithelial Cancers. Korean J Pathol, 2012. 46(6): p. 532-40. 32. Ramani, P., A. Headford, and M.T. May, GLUT1 protein expression correlates with unfavourable histologic category and high risk in patients with neuroblastic tumours. Virchows Arch, 2013. 462(2): p. 203-9. 33. Shim, B.Y., et al., Glucose transporter 1 (GLUT1) of anaerobic glycolysis as predictive and prognostic values in neoadjuvant chemoradiotherapy and laparoscopic surgery for locally advanced rectal cancer. Int J Colorectal Dis, 2013. 28(3): p. 375-83. 34. Jozwiak, P., et al., Expression of hypoxia-related glucose transporters GLUT1 and GLUT3 in benign, malignant and non-neoplastic thyroid lesions. Mol Med Rep, 2012. 6(3): p. 601-6. 35. Airley, R., et al., Glucose transporter Glut-1 is detectable in peri-necrotic regions in many human tumor types but not normal tissues: Study using tissue microarrays. Ann Anat, 2010. 192(3): p. 133-8. 36. Airley, R., et al., Glucose transporter glut-1 expression correlates with tumor hypoxia and predicts metastasis-free survival in advanced carcinoma of the cervix. Clin Cancer Res, 2001. 7(4): p. 928-34. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/59052 | - |
dc.description.abstract | 目的:
本研究的目標是找出子宮內膜癌(endometrial endometrioid adenocarcinoma ,EEC)在癌化、侵襲及轉移過程中的相關基因。 材料方法: 手術取得了十個子宮內膜正常的患者(normal endometria, NEM)、十個非典型子宮內膜增生患者(atypical endometrial hyperplasia, AEH)及八十五個子宮內膜癌患者(EEC)的子宮內膜組織檢體。利用GeneChip Array各別分析正常、非典型子宮內膜增生及子宮內膜癌的基因表現圖譜。接著,利用半定量反轉錄聚合酶鍊鎖反應(semi-quantitative reverse transcriptase PCR ,SQ RT-PCR)與定量反轉錄聚合酶鍊鎖反應(real time reverse transcriptase PCR ,RTQ RT-PCR),找出與臨床病理及生存因子之間相關的基因。 結果: 進行微陣列分析之後,利用半定量聚合酶鍊鎖反應,我們挑選出七個候選基因。接著根據先前文獻之報導,挑選其中三個可能為致癌基因的候選者,包含PSAT1、SLC2A1及PLOD2做後續之分析。在八十五個子宮內膜癌患者中,PLOD2在子宮內膜癌(EEC)相對於正常子宮內膜(NEM)及非典型子宮內膜增生(AEH)的表現量顯著較高(p=0.024);而在具較深的肌層侵入、組織分級較高、分化較不完全的子宮內膜癌患者中,也發現了比較高的PLOD2表現量(肌層侵入較深:p=0.041;腫瘤組織分級較高:p=0.039)。此外,其餘兩個基因則在所有分析的臨床病理參數上,都沒有看到其基因表現量與臨床表現具顯著之關係。 結論: PLOD2可能是子宮內膜癌在癌化過程中扮演著致癌基因的重要角色。然而,其表現量僅與癌化過程及部分病理參數相關,並未與其他臨床表現具明顯之相關性。未來可能需要增大檢體量進一步驗證、或同時再找尋並深入研究其他基因,用以找出在子宮內膜癌的癌化過程、進展及轉移中具關鍵角色,並能用於病患的診斷預後之基因。 | zh_TW |
dc.description.abstract | Abstract
OBJECTIVE: This study aimed to identify the dysregulated genes involved in the tumorigenesis and progression of endometrial endometrioid adenocarcinoma (EEC). METHODS: Specimens of endometrial tissues included 10 normal endometria (NEM), 10 atypical endometrial hyperplasia (AEH), and 85 EEC. The expression profiles were compared using GeneChip Array. The gene expression levels were determined by semi-quantitative reverse transcriptase PCR (SQ RT-PCR) in 16 samples and real-time reverse transcriptase PCR (RTQ RT-PCR) in 85 EEC patients. The correlations between the expression of dysregulated genes and clinico-pathologic parameters including survival analysis of EEC were evaluated. Results: Seven dysregulated genes were first identified through microarray and SQ RT-PCR. Three genes, including PSAT1, SLC2A1, and PLOD2, which were selected according to past reports, underwent subsequent analysis. In our samples, the relative expression levels of PLOD2 (p=0.024) were higher in EEC than in NEM and AEH. The PLOD2 expression levels were higher in EEC with deep myometrial invasion (p=0.041) and higher histologic grade (p=0.039), but had no difference with other clinico-pathologic parameters. The expression levels of two other genes with clinico-pathologic parameters were not different significantly. Conclusions: PLOD2 might be a dysregulated gene in the tumorigenesis of EEC, but it is not involved in all bio-pathologic features. In the future, we might need to collect more patients to conduct further analysis, or try to investigate other candidates to find genes that may be potential molecular markers for clinical prognosis while detecting the disease and the progression of EEC. | en |
dc.description.provenance | Made available in DSpace on 2021-06-16T08:47:00Z (GMT). No. of bitstreams: 1 ntu-102-P00448010-1.pdf: 2477022 bytes, checksum: 79aaf83880db06e80705b871af5e8b4a (MD5) Previous issue date: 2013 | en |
dc.description.tableofcontents | 目 錄
口試委員會審定書………………………………………………………………… i 誌謝……………………………………………………………………………… ii 中文摘要……………………………………………………………………… iii 英文摘要………………………………………………………………………… v 前言………………………………………………………………… 1 材料方法………………………………………………………………… 3 結果…………………………………………………………………… 8 討論…………………………………………………………………… 12 參考文獻………………………………………………………………………… 17 附錄……………………………………………………………………………... 34 | |
dc.language.iso | zh-TW | |
dc.title | PLOD2表現與子宮內膜癌在癌化、侵襲及轉移關係之探討 | zh_TW |
dc.title | Correlation between PLOD2 expression level and the tumorigenesis and progression of endometrial endometrioid adenocarcinoma | en |
dc.type | Thesis | |
dc.date.schoolyear | 101-2 | |
dc.description.degree | 碩士 | |
dc.contributor.coadvisor | 鄭文芳(Wen-Fang Cheng) | |
dc.contributor.oralexamcommittee | 陳祈安(Chi-An Chen) | |
dc.subject.keyword | 子宮內膜癌,致癌基因,腫瘤生成,原膠原離胺酸, | zh_TW |
dc.subject.keyword | Endometrial endometrioid adenocarcinoma,oncogene,carcinogenesis,PLOD2, | en |
dc.relation.page | 38 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2013-08-20 | |
dc.contributor.author-college | 醫學院 | zh_TW |
dc.contributor.author-dept | 分子醫學研究所 | zh_TW |
顯示於系所單位: | 分子醫學研究所 |
文件中的檔案:
檔案 | 大小 | 格式 | |
---|---|---|---|
ntu-102-1.pdf 目前未授權公開取用 | 2.42 MB | Adobe PDF |
系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。