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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 郭柏齡 | |
dc.contributor.author | Pei-Chain Chih | en |
dc.contributor.author | 計佩岑 | zh_TW |
dc.date.accessioned | 2021-06-16T08:45:01Z | - |
dc.date.available | 2018-09-02 | |
dc.date.copyright | 2013-09-02 | |
dc.date.issued | 2013 | |
dc.date.submitted | 2013-08-22 | |
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dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/59021 | - |
dc.description.abstract | T cell migration plays an important role in inflammation and wound healing. They circulate in the blood and migrate into different tissues to execute their tasks. In this study, we investigated the effects of substrate stiffness, cell activation, diabetes mellitus (DM), and hydrostatic pressure on T cell migration. We hypothesized that stiffer substrates and application of hydrostatic pressure increase the speed of T cell migration, and that defect in T cell activation and DM condition decrease the migration speed. Mouse QL9 T cells were used and live cell images were taken to analyze the migration speed. Polyacrylamide (PA) gels of stiffness varying from 1 to 34 kPa and with graded stiffness were used to study the effects of substrate stiffness on T cell migration. Poly(dimethylsiloxane) (PDMS)-based cell chips were fabricated to apply a 10 cmH2O hydrostatic pressure to the cultured cells. The DM condition was simulated using STZ-treated mice. The T cell activation was modulated via application of interleukin-2 (IL-2) and defect of cell activation was simulated by knocking out CD28, an important cell-membrane receptor for T cell activation. Since application of the hydrostatic pressure may affect the traffic of water through cell membrane via aquaporin1 (AQP1), which has been shown to play an important role in cell migration, we also examined whether application of hydrostatic pressure facilitated the expression of AQP1. Our results indicate that the T cell migration was not sensitive to the variation of substrate stiffness. Application of a 10 cmH2O hydrostatic pressure may enhance the migration speed, but the difference was not significant when compared with cells without pressure exposure. Application of the 10 cmH2O enhanced AQP1 expression, but the relationships among AQP1 expression, migration speed, and hydrostatic pressure require further studies to clarify. DM condition and knockout of CD28 appeared to decrease the migration speed, but the difference was either not significant when compared with control. | en |
dc.description.provenance | Made available in DSpace on 2021-06-16T08:45:01Z (GMT). No. of bitstreams: 1 ntu-102-R00945015-1.pdf: 2448445 bytes, checksum: a132ec0c50195cdbecbc3412dd0b9eda (MD5) Previous issue date: 2013 | en |
dc.description.tableofcontents | 誌謝 i
中文摘要 iii ABSTRACT v CONTENTS vii LIST OF FIGURES ix LIST OF TABLES xiii Chapter 1 Introduction 1 1.1 Cell migration 1 1.2 T cell 2 1.3 The purpose of this study 4 Chapter 2 Materials and Methods 11 2.1 Stiffness substrate part 11 2.1.1 Coverslip Surface Modification 11 2.1.2 Polymerization of Polyacrylamide Gels 12 2.1.3 Functionalization of Polyacrylamide Gels 14 2.1.4 Real-time observation of cell migration 16 2.2 Hydrostatic pressure, cell activation and DM 16 2.2.1 Device design 16 2.2.2 Soft lithography 17 2.2.3 Real-time observation of cell migration 18 2.2.4 Immunofluorescence 20 2.3 Cell Culture 22 2.3.1 2C CTLs. 22 2.3.2 Generation of 2C CTLs 22 2.3.3 Diabetes induction and monitoring 23 2.4 Cell migration analysis 23 2.5 Statistical analysis 25 Chapter 3 Results and Discussion 26 3.1 Substrate stiffness experiments 26 3.1.1 Cell migration on uniform-compliant gels PA gels 26 3.1.2 Cell migration on gradient-compliant PA gels 29 3.2 Cell activation and DM 32 3.2.1 The effects of cell activation on T cell migration speed 32 3.2.2 The effects of DM on T cell migration speed 34 3.3 T cell migration under hydrostatic pressure 35 3.3.1 The effects of hydrostatic pressure on T cell migration speed 35 3.3.2 The Expression of AQP1 on NIH 3T3 fibroblasts under hydrostatic pressure 40 Chapter 4 Conclusion and Future Works 42 REFERENCE 43 | |
dc.language.iso | en | |
dc.title | 基質硬度、靜水壓、細胞活化與糖尿病對T細胞移動的影響 | zh_TW |
dc.title | The Effects of Substrate Stiffness, Hydrostatic Pressure, Cell Activation and Diabetes Mellitus on T Cell Migration | en |
dc.type | Thesis | |
dc.date.schoolyear | 101-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 陳淑靜,李超煌 | |
dc.subject.keyword | T細胞,細胞移動,基質硬度,水膠,靜水壓,細胞活化,糖尿病,水通道蛋白1, | zh_TW |
dc.subject.keyword | T cell,cell migration,substrate stiffness,PA gels,hydrostatic pressure,cell activation,diabetes mellitus,aquaporin1, | en |
dc.relation.page | 46 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2013-08-22 | |
dc.contributor.author-college | 電機資訊學院 | zh_TW |
dc.contributor.author-dept | 生醫電子與資訊學研究所 | zh_TW |
顯示於系所單位: | 生醫電子與資訊學研究所 |
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