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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 潘建源(Chien-Yuan Pan) | |
dc.contributor.author | Shi-Zhen Liu | en |
dc.contributor.author | 劉士禎 | zh_TW |
dc.date.accessioned | 2021-06-16T08:36:43Z | - |
dc.date.available | 2014-01-27 | |
dc.date.copyright | 2014-01-27 | |
dc.date.issued | 2013 | |
dc.date.submitted | 2013-11-04 | |
dc.identifier.citation | Aggarwal, B. B., A. Kumar, and A. C. Bharti. 'Anticancer Potential of Curcumin: Preclinical and Clinical Studies.' Anticancer Res 23, no. 1A (Jan-Feb 2003): 363-98.
Balch, W. E., R. I. Morimoto, A. Dillin, and J. W. Kelly. 'Adapting Proteostasis for Disease Intervention.' [In English]. Science 319, no. 5865 (Feb 15 2008): 916-19. Bandyopadhyay, U., S. Kaushik, L. Varticovski, and A. M. Cuervo. 'The Chaperone-Mediated Autophagy Receptor Organizes in Dynamic Protein Complexes at the Lysosomal Membrane.' Mol Cell Biol 28, no. 18 (Sep 2008): 5747-63. Becker, D. E. 'Drug Therapy in Dental Practice: General Principles. Part 2 - Pharmacodynamic Considerations.' Anesth Prog 54, no. 1 (Spring 2007): 19-23; quiz 24-5. Bjorkoy, G., T. Lamark, A. Brech, H. Outzen, M. Perander, A. Overvatn, H. Stenmark, and T. Johansen. 'P62/Sqstm1 Forms Protein Aggregates Degraded by Autophagy and Has a Protective Effect on Huntingtin-Induced Cell Death.' J Cell Biol 171, no. 4 (Nov 21 2005): 603-14. Dorai, T., and B. B. Aggarwal. 'Role of Chemopreventive Agents in Cancer Therapy.' Cancer Lett 215, no. 2 (Nov 25 2004): 129-40. Dziedzic, S. A., and A. B. Caplan. 'Identification of Autophagy Genes Participating in Zinc-Induced Necrotic Cell Death in Saccharomyces Cerevisiae.' Autophagy 7, no. 5 (May 2011): 490-500. Eng, K. E., M. D. Panas, G. B. Karlsson Hedestam, and G. M. McInerney. 'A Novel Quantitative Flow Cytometry-Based Assay for Autophagy.' Autophagy 6, no. 5 (Jul 2010): 634-41. Eskelinen, E. L. 'New Insights into the Mechanisms of Macroautophagy in Mammalian Cells.' Int Rev Cell Mol Biol 266 (2008): 207-47. Fan, F., and K. V. Wood. 'Bioluminescent Assays for High-Throughput Screening.' Assay Drug Dev Technol 5, no. 1 (Feb 2007): 127-36. Gould, S. J., and S. Subramani. 'Firefly Luciferase as a Tool in Molecular and Cell Biology.' Anal Biochem 175, no. 1 (Nov 15 1988): 5-13. Higashiyama, T., R. Inatsugi, S. Sakamoto, N. Sasaki, T. Mori, H. Kuroiwa, T. Nakada, et al. 'Species Preferentiality of the Pollen Tube Attractant Derived from the Synergid Cell of Torenia Fournieri.' Plant Physiol 142, no. 2 (Oct 2006): 481-91. Homma, K., K. Suzuki, and H. Sugawara. 'The Autophagy Database: An All-Inclusive Information Resource on Autophagy That Provides Nourishment for Research.' Nucleic Acids Res 39, no. Database issue (Jan 2011): D986-90. Hsiao, W. L., and L. Liu. 'The Role of Traditional Chinese Herbal Medicines in Cancer Therapy--from Tcm Theory to Mechanistic Insights.' Planta Med 76, no. 11 (Aug 2010): 1118-31. Iwai-Kanai, E., H. Yuan, C. Huang, M. R. Sayen, C. N. Perry-Garza, L. Kim, and R. A. Gottlieb. 'A Method to Measure Cardiac Autophagic Flux in Vivo.' Autophagy 4, no. 3 (Apr 2008): 322-9. Lai, Y. H., S. L. Yu, H. Y. Chen, C. C. Wang, H. W. Chen, and J. J. Chen. 'The Hlj1-Targeting Drug Screening Identified Chinese Herb Andrographolide That Can Suppress Tumour Growth and Invasion in Non-Small-Cell Lung Cancer.' Carcinogenesis 34, no. 5 (May 2013): 1069-80. Leone, M., D. Zhai, S. Sareth, S. Kitada, J. C. Reed, and M. Pellecchia. 'Cancer Prevention by Tea Polyphenols Is Linked to Their Direct Inhibition of Antiapoptotic Bcl-2-Family Proteins.' Cancer Res 63, no. 23 (Dec 1 2003): 8118-21. Levine, B., and D. J. Klionsky. 'Development by Self-Digestion: Molecular Mechanisms and Biological Functions of Autophagy.' Dev Cell 6, no. 4 (Apr 2004): 463-77. Levine, B., N. Mizushima, and H. W. Virgin. 'Autophagy in Immunity and Inflammation.' Nature 469, no. 7330 (Jan 20 2011): 323-35. Li, S., and B. Zhang. 'Traditional Chinese Medicine Network Pharmacology: Theory, Methodology and Application.' Chin J Nat Med 11, no. 2 (Mar 2013): 110-20. Li, W. W., J. Li, and J. K. Bao. 'Microautophagy: Lesser-Known Self-Eating.' [In English]. Cellular and Molecular Life Sciences 69, no. 7 (Apr 2012): 1125-36. Lin, N. Y., C. Beyer, A. Giessl, T. Kireva, C. Scholtysek, S. Uderhardt, L. E. Munoz, et al. 'Autophagy Regulates Tnfalpha-Mediated Joint Destruction in Experimental Arthritis.' Ann Rheum Dis 72, no. 5 (May 2013): 761-8. Lin, Y., N. Peng, J. Li, H. Zhuang, and Z. C. Hua. 'Herbal Compound Triptolide Synergistically Enhanced Antitumor Activity of Amino-Terminal Fragment of Urokinase.' Mol Cancer 12 (2013): 54. Liu, A. L., and G. H. Du. '[Network Pharmacology: New Guidelines for Drug Discovery].' Yao Xue Xue Bao 45, no. 12 (Dec 2010): 1472-7. Lu, Q., and J. G. Jiang. 'Chemical Metabolism of Medicinal Compounds from Natural Botanicals.' [In English]. Current Medicinal Chemistry 19, no. 11 (Apr 2012): 1682-705. Mizukami, K., T. Asada, T. Kinoshita, K. Tanaka, K. Sonohara, R. Nakai, K. Yamaguchi, et al. 'A Randomized Cross-over Study of a Traditional Japanese Medicine (Kampo), Yokukansan, in the Treatment of the Behavioural and Psychological Symptoms of Dementia.' Int J Neuropsychopharmacol 12, no. 2 (Mar 2009): 191-9. Moscat, J., and M. T. Diaz-Meco. 'P62 at the Crossroads of Autophagy, Apoptosis, and Cancer.' Cell 137, no. 6 (Jun 12 2009): 1001-4. Nakaso, K., Y. Yoshimoto, T. Nakano, T. Takeshima, Y. Fukuhara, K. Yasui, S. Araga, et al. 'Transcriptional Activation of P62/A170/Zip During the Formation of the Aggregates: Possible Mechanisms and the Role in Lewy Body Formation in Parkinson's Disease.' Brain Res 1012, no. 1-2 (Jun 25 2004): 42-51. Patel, A. S., L. Lin, A. Geyer, J. A. Haspel, C. H. An, J. Cao, I. O. Rosas, and D. Morse. 'Autophagy in Idiopathic Pulmonary Fibrosis.' PLoS One 7, no. 7 (2012): e41394. Peracchio, C., O. Alabiso, G. Valente, and C. Isidoro. 'Involvement of Autophagy in Ovarian Cancer: A Working Hypothesis.' J Ovarian Res 5, no. 1 (2012): 22. Shimomura, O. 'Bioluminescence in the Sea: Photoprotein Systems.' Symp Soc Exp Biol 39 (1985): 351-72. Shimomura, O., F. H. Johnson, and Y. Saiga. 'Extraction, Purification and Properties of Aequorin, a Bioluminescent Protein from Luminous Hydromedusan, Aequorea.' [In English]. Journal Of Cellular And Comparative Physiology 59, no. 3 (1962): 223-&. Su, H., F. Li, M. J. Ranek, N. Wei, and X. Wang. 'Cop9 Signalosome Regulates Autophagosome Maturation.' Circulation 124, no. 19 (Nov 8 2011): 2117-28. Susana Castro-Obregon, Ph.D. 'The Discovery of Lysosomes and Autophagy.' Nature Education 3, no. 9 (2010): 49. Tang, Z. P., Y. D. Xu, J. R. Peng, Y. K. Ou, and Z. Guan. '[Mechanism of Radiosensitization of 3-Methyladenine in Human Hypopharynx Cancer Cells].' Zhonghua Er Bi Yan Hou Tou Jing Wai Ke Za Zhi 47, no. 11 (Nov 2012): 937-41. Tanida, I., T. Ueno, and E. Kominami. 'Lc3 Conjugation System in Mammalian Autophagy.' Int J Biochem Cell Biol 36, no. 12 (Dec 2004): 2503-18. Tsai, M. L., C. C. Lin, W. C. Lin, and C. H. Yang. 'Antimicrobial, Antioxidant, and Anti-Inflammatory Activities of Essential Oils from Five Selected Herbs.' Biosci Biotechnol Biochem 75, no. 10 (2011): 1977-83. Tsien, R. Y. 'The Green Fluorescent Protein.' [In English]. Annual Review Of Biochemistry 67 (1998): 509-44. Wang, I. F., B. S. Guo, Y. C. Liu, C. C. Wu, C. H. Yang, K. J. Tsai, and C. K. J. Shen. 'Autophagy Activators Rescue and Alleviate Pathogenesis of a Mouse Model with Proteinopathies of the Tar DNA-Binding Protein 43.' [In English]. Proceedings of the National Academy of Sciences of the United States of America 109, no. 37 (Sep 11 2012): 15024-29. Wu, C., C. Suzuki-Ogoh, and Y. Ohmiya. 'Dual-Reporter Assay Using Two Secreted Luciferase Genes.' Biotechniques 42, no. 3 (Mar 2007): 290, 92. Wu, Y. T., H. L. Tan, G. Shui, C. Bauvy, Q. Huang, M. R. Wenk, C. N. Ong, P. Codogno, and H. M. Shen. 'Dual Role of 3-Methyladenine in Modulation of Autophagy Via Different Temporal Patterns of Inhibition on Class I and Iii Phosphoinositide 3-Kinase.' J Biol Chem 285, no. 14 (Apr 2 2010): 10850-61. Zughaier, S., P. Karna, D. Stephens, and R. Aneja. 'Potent Anti-Inflammatory Activity of Novel Microtubule-Modulating Brominated Noscapine Analogs.' PLoS One 5, no. 2 (2010): e9165. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/58885 | - |
dc.description.abstract | 許多傳統漢醫使用的草藥萃取物,具有開發調控細胞自噬新藥的潛力。細胞自噬是種依靠溶酶體(lysosome)的降解機制。在缺乏養分,逆境與細胞凋亡時扮演重要的角色。本研究使用 Irp62/HEK(接合水母冷光酶之複合蛋白)細胞株,建立篩選細胞自噬調控因子的平台,來研究草藥萃取物對細胞自噬的影響。以雷帕黴素(rapamycin)作為促進劑;6-氨基-3-甲基嘌呤(3-methyladenine)作為抑制劑。在497個草藥萃取物中,有14個可以促進細胞自噬。再使用MTT assay測試此14個潛力萃取物的毒性,挑選萃取自母草屬的HE717,來進行更進一步的測試。以臺盼藍細胞存活率分析法來驗證MTT assay的結果。而活化細胞自噬的能力,則是分別透過西方墨點法對p62、流式細胞儀與螢光顯微鏡對 GFP-LC3的影響來確認。綜合以上結果,由於 HE717具細胞自噬的活化能力,因此HE717具有製成藥物的潛力。本研究開發的生物篩選平台,是第一個用來篩選草藥對細胞自噬影響的平台。另外,研究結果也表示,母草屬的萃取物可能具有活化細胞自噬的效果。 | zh_TW |
dc.description.abstract | Several herbal extracts (HEs), used in traditional Chinese medicine, have potential for the purpose of autophagy regulation. Autophagy is a lysosome-dependent degradation pathway. It plays important roles in starvation, stress response and apoptosis. To establish the platform to validate the modulators of autophagy, Irp62/HEK, which contain the constitutively expressed p62-luciferase for detection cells were used to detect the effects of herbal extracts on autophagy. Rapamycin was used as the activator control, and 3-methyladenine (3-MA) was used as the inhibitor control. Among the 497 HEs screened by the luciferase assay, 14 could serve as the activator of autophagy. Among 14 HEs, MTT assay was used to test the toxicity by measure the viability of the cells, and HE717 was chosen for the further study. The low toxicity of HE717 was carried out by trypan blue exclusion assay. The effect of activators was validated by Western blot of p62, flow cytometer measurement and fluorescence puncta of GFP-LC3. Taken together, HE717, isolated from whole plant of Lindernia sp., has the potential for drug development due to its activator effect of autophagy. This was the first platform to screen autophagy modulators from herbal library and the first report that Lindernia sp. could activate autophagy through p62 and LC3. | en |
dc.description.provenance | Made available in DSpace on 2021-06-16T08:36:43Z (GMT). No. of bitstreams: 1 ntu-102-R99b41026-1.pdf: 3395037 bytes, checksum: f7d07b4d29cd0a08b10180c30cc935b1 (MD5) Previous issue date: 2013 | en |
dc.description.tableofcontents | 口試委員會審定書 #
摘要 i ABSTRACT ii CONTENTS iii LIST OF TABLES iv LIST OF FIGURES v ABBREVIATION vi Chapter 1 Introduction 1 Chapter 2 Material and Methods 8 Chapter 3 Results 14 Chapter 4 Discussion 19 Chapter 5 Conclusion 22 REFERENCE 36 APPENDIX 41 | |
dc.language.iso | en | |
dc.title | 從中草藥萃取物庫中篩選具調控細胞自噬作用的調節劑 | zh_TW |
dc.title | Screening for autophagy modulators from an herbal extract library | en |
dc.type | Thesis | |
dc.date.schoolyear | 102-1 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 齊肖琪(Shau-Chi Chi),廖永豐(Yung-Feng Liao) | |
dc.subject.keyword | 草藥萃取物,細胞自噬,p62,LC3,篩選平台,母草屬, | zh_TW |
dc.subject.keyword | herbal extract,autophagy,p62,LC3,Lindernia,screening platform, | en |
dc.relation.page | 45 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2013-11-04 | |
dc.contributor.author-college | 生命科學院 | zh_TW |
dc.contributor.author-dept | 生命科學系 | zh_TW |
顯示於系所單位: | 生命科學系 |
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