龍葵葉片95%酒精萃取物之抗氧化能力及抗醣化作用分析

Abstract

最終醣化蛋白(advanced glycation end-products, AGEs)的形成，導致發炎反應的發生與胰島素抗性(insulin resistance)的產生。有證據顯示抗氧化劑可以抑制活性氧(reactive oxygen species, ROS)的形成，經由抑制糖基化(glycation)減少醣化終產物的生成。龍葵(Solanum nigrum)可以作為改善血糖的藥用食物，然而其活性成分與作用機轉仍然不清楚。本研究結果顯示，龍葵95%乙醇萃取物的抗氧化活性高於龍葵50%乙醇萃取物和龍葵水萃取物。此外，龍葵95%乙醇萃取物具有抑制糖基化的活性，可以抑制果糖胺(fructosamine)與α-二羰基化合物(α-dicarbonylcompiund)的生成。在每毫克龍葵95%乙醇萃取物中，solasonine與solamargine的量分別為0.484毫克和0.183毫克。基於上述，龍葵具備當作萃取抗糖基化和抗糖尿病活性成分的潛力。

Solanum nigrum are recently not only considered to be the brand-new source of functional ingredients that exerts antiglycation and anti-diabetes activities but also to be kind of medicinal food for improving blood glucose. However, there are only few identities of the active compounds and how those above-mentioned compounds counteract diabetes are still unknown. To induces insulin resistance occurs in an inflammatory response that requires the hyperglycemia results in the formation of advanced glycation end-products (AGEs). Evidence indicates that antioxidants can suppress the formation of reactive oxygen species, decrease levels of AGEs by inhibiting glycation. In this research demonstrates that 95% ethanolic extracts of Solanum nigrum exerted significant antioxidative activity compared with 50% ethanolic extracts and aqueous extracts. Moreover, 95% ethanolic extracts of S. nigrum produced antiglycative activity, which contributed to the inhibition of fructosamine and generation of α-dicarbonyl compounds. The results indicate that 95% ethanolic extracts of S. nigrum are with great bioactivities on antiglycation and anti-diabetes.