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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
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dc.contributor.advisor | 王淑珍(Shu-Jen Wang) | |
dc.contributor.author | Po-Chun Lin | en |
dc.contributor.author | 林柏均 | zh_TW |
dc.date.accessioned | 2021-06-16T08:21:09Z | - |
dc.date.available | 2019-03-18 | |
dc.date.copyright | 2014-03-18 | |
dc.date.issued | 2014 | |
dc.date.submitted | 2014-01-28 | |
dc.identifier.citation | 陳薌雯 (2012) 生長素、一氧化氮與oxylipins共同參與調控光照誘導水稻種子波動狀根發生。國立臺灣大學生物資訊暨農學院系碩士論文。
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dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/58589 | - |
dc.description.abstract | 在印度塔爾沙漠被發現的印度梨形孢真菌 (Piriformospora indica, P. indica) 為對植物具有正面效益的益生真菌,雖然在其他植物中已有許多研究觀察到P. indica之效用,但是詳細的機制仍需進一步研究。目前相關研究大多還是以阿拉伯芥為主,然而發表在大麥等穀類作物的研究逐年增加,說明此真菌具提升糧食作物生長勢之潛力。本研究針對水稻品種臺中在來一號 (Oryza sativa L. cv. Taichung Native 1, TCN1),觀察真菌寄宿對其所產生之影響,結果發現接種P. indica使得TCN1之主根變短,而不定根的長度及數目則是有增加之趨勢。此外,雖主根上的側根數目減少,但側根長度卻仍呈現增長之現象。除了發現根系結構受P. indica寄宿而產生改變外,亦能觀察其地上部及根部鮮重有提升之趨勢。進一步觀察對植體內生長素 (auxin) 及乙烯 (ethylene) 反應之基因表現變化,發現這些基因表現皆會因接種P. indica而被誘導提升,推測生長素和乙烯可能參與在P. indica改變根系之調控機制中。另外在鹽分及乾旱逆境的實驗中說明,接種P. indica能維持寄主在逆境中的生長,結果指出P. indica可能透由抗氧化酵素活性的提升來清除逆境下生成的活性氧化物 (reactive oxygen spices, ROS),增加水稻對逆境之耐受性。 | zh_TW |
dc.description.abstract | Piriformospora indica is a beneficial endophytic fungi for plants. Positive effects of P. indica on plant growth have been observed in several plant species, the mechanism of P. indica-promoted plant growth still need to be revealed. So far, most of the physiological responses of plants colonized by P. indica was observed in Arabidopsis. Some researches related to P. indica effect on cereal crops such as barley were published, and it was implied that P. indica colonization can be a powerful approach to improve crop production. Our works presented here was focused on the effect of P. indica on rice (Oryza sativa L. cv. Taichung Native 1, TCN1) growth and the abiotic stress tolerance. The result showed that P. indica reduced primary roots elongation. In addition, the total number and length of adventitious roots were also increased. Although the total number of lateral roots on primary roots was decreased, the length of total lateral roots was increased. In addition to the change of root architecture, the fresh weight of root systems was significantly increased in seedlings colonized by P. indica. Furthermore, we found that auxin- and ethylene-responsive genes in P. indica–colonized roots were up-regulated. Therefore, the level of auxin and ethylene might increase in colonized roots and participated in the P. indica-induced physiological pathway, but it still need some further study to confirm. Moreover, P. indica could help TCN1 to reduce the damage caused by salinity and drought stresses. Results showed that P. indica could up-regulate the antioxidant enzyme activities under stresses and environment recovery stage to enhance stress tolerance of rice plants. | en |
dc.description.provenance | Made available in DSpace on 2021-06-16T08:21:09Z (GMT). No. of bitstreams: 1 ntu-103-R00621118-1.pdf: 2507818 bytes, checksum: 41502b2a8f41067b2e92f313f96d1493 (MD5) Previous issue date: 2014 | en |
dc.description.tableofcontents | Abstract………………………………………………………………………………i
中文摘要…………………………………………………………………………ii 目錄……………………………………………………………………………….....iii 圖目錄……………………………………………………………………………v 附圖目錄…………………………...…………………………………………….….v 縮寫字對照………………………...…………………………………………….….vi 前言…………….………………………….…………………………………………..1 1. 內生菌根菌與植物間之互利共生………………………………..………….1 2. 印度梨形孢真菌之介紹……………………….…………….………………2 3. 印度梨形孢真菌調控植物體內的賀爾蒙………………………………..2 4. 印度梨形孢真菌的寄宿提升植物耐逆境之能力……………….……….3 5. 印度梨形孢真菌在水稻中之研究…….…………………………....……..…5 5. 本論文研究主題…………………….…………………………....……..…5 材料與方法…….………………………………….…………………………..…..6 植物材料.………………………………………………………………….……6 Piriformospora indica繼代之方式.……………………………………….……6 水稻栽培以及接種P. indica 的方式……………..………………….………..7 鹽分逆境之處理………………………………………………………………..8 脫水逆境之處理.…………………………..…………………………………..8 p-chlorophenoxyisobutyric acid (PCIB) 抑制劑之處理………………..……..8 P. indica細胞壁的萃取液…………………………….………………………..8 酵素活性之測定…………………………………………………….………..9 Catalase (CAT) activity analysis……………………...………..………..9 Ascorbate peroxidase (APX) activity analysis………………………..……..9 Glutathione reductase (GR) activity analysis…….…….……...…………..9 Superoxide dismutase (SOD) activity analysis…………………..…..……..10 葉綠素含量分析………………………………………………….……………10 RNA之萃取…………………………………………..…………………………11 TURBO DNase處理……………………………………….……………………11 RNA電泳………………………………………………….………………….…12 即時反轉錄聚合酶鏈鎖反應 (real-time RT-PCR)………………..…..……12 結果…………….……………………………………………………….…………....13 印度梨形孢真菌 (Piriformospora indica) 提升水稻生長勢並改變其根系結構………………………………………………………………….………..…..13 印度梨形孢真菌透由生長素之調控改變根系結構………………..……….....13 印度梨形孢真菌的寄宿能增進水稻耐鹽分逆境之能力………….……….…14 印度梨形孢真菌的寄宿促進水稻耐旱能力……………………….…………..16 討論…………….………………………………………………………….…………17 接種印度梨形孢真菌使水稻根系型態發生改變………………….…….…....17 接種印度梨形孢真菌與其細胞壁萃取液對主根型態影響之異同..…….…....17 印度梨形孢的寄宿與生長素之關聯……………….................……..…………17 生長素與乙烯之交互作用………………………………………….…….……18 印度梨形孢之寄宿提升水稻在逆境下之耐受性………………….….………18 結語及未來展望…………………………………………………….……..…...19 參考文獻………………………………………………………..…………………...33 圖目錄 圖一、 TCN1水稻幼苗接種印度梨形孢真菌5天後之生長分析............................21 圖二、施加生長素作用抑制劑PCIB後,接種P. indica後對水稻TCN1之影響。...................................................................................................................22 圖三、施加生長素作用抑制劑PCIB後,TCN1水稻根部基因表現之變化。.......23 圖四、 TCN1水稻幼苗接種印度梨形孢真菌兩天後再施加生長素抑制劑PCIB之生長情形。………………………………………………………………24 圖五、 TCN1水稻幼苗接種印度梨形孢真菌處理150 mM NaCl之生長分析。...25 圖六、TCN1水稻幼苗接種印度梨形孢真菌處理150 mM NaCl之地下部以及第四片葉之抗氧化酵素活性。………………………….…..............……………26 圖七、TCN1水稻幼苗接種印度梨形孢真菌處理鹽分逆境後,移至正常生長環境中復原,並進行生長分析。............................................................……27 圖八、TCN1水稻幼苗接種印度梨形孢真菌鹽逆境後復原10天後之地下部以及第四片葉之抗氧化酵素活性。....................................................……28 圖九、脫水逆境下印度梨形孢對TCN1秈稻之影響。..............................................29 圖十、脫水逆境下印度梨形孢對TNG67梗稻之影響。......………………………30 附圖目錄 附圖一、印度梨形孢真菌之細胞壁萃取液對於TCN1水稻幼苗植株之影響。......................................................................................................31 附圖二、印度梨形孢真菌在水稻植株內影響生長素與乙烯交互作用可能之途徑……………………………………………………………………….32 | |
dc.language.iso | zh-TW | |
dc.title | 印度梨型孢真菌可增進水稻生長及鹽分/乾旱逆境之耐受性 | zh_TW |
dc.title | Piriformospora indica Increases Rice Growth and Salt/Drought Stress Tolerance | en |
dc.type | Thesis | |
dc.date.schoolyear | 102-1 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 張孟基(Men-Chi Chang),鄭秋萍(Chiu-Ping Cheng),劉啟德(Chi-Te Liu),陳仁治(Jen-Chih Chen) | |
dc.subject.keyword | 水稻 (Oryza sativa L.),印度梨形孢真菌 (Piriformospora indica),鹽分逆境,乾旱逆境, | zh_TW |
dc.subject.keyword | rice (Oryza sativa L.),Piriformospora indica,salt stress,drought stress, | en |
dc.relation.page | 36 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2014-01-29 | |
dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
dc.contributor.author-dept | 農藝學研究所 | zh_TW |
顯示於系所單位: | 農藝學系 |
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