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標題: | 新穎轉錄因子參與阿拉伯芥抵抗灰黴病菌之研究 Identification of novel transcription factors involved in the Arabidopsis defense response to Botrytis cinerea |
作者: | Yu-Pin Lu 呂毓蘋 |
指導教授: | 金洛仁(Laurent Zimmerli) |
關鍵字: | 灰黴病菌,防禦反應,乙烯反應轉錄因子,活性氧物種,阿拉伯芥, Botrytis cinerea,defense response,ERF,ROS,Arabidopsis, |
出版年 : | 2014 |
學位: | 碩士 |
摘要: | 灰黴病菌是(Botrytis cinerea)一種會感染多種作物的腐生型真菌。灰黴病菌會刺激植物宿主產生大量活性氧物種來促進宿主細胞死亡進而造成感染。在阿拉伯芥中,茉莉酸及乙烯訊號傳遞路徑對於抵抗灰黴病菌扮演重要的角色,而乙烯反應轉錄因子(ETHYLENE RESPONSE FACTORs, ERFs)也參與其中。藉由篩選過度表現轉錄因子的種子庫,我們發現阿拉伯芥ERF19在抵抗灰黴病菌中扮演重要腳色。ERF19是屬於ERF/AP2轉錄因子中的DREB亞家族。ERF19過度表現植株在感染灰黴病菌後呈現較嚴重的壞死病徵,並且茉莉酸/乙烯路徑中的植物防禦素PDF1.2的基因表現量也較低。再者,ERF19過度表現植株累積更多的活性氧物種。綜合而言,我們研究結果顯示ERF19不但參與了茉莉酸/乙烯路徑的調控,也可能影響活性氧物種的累積。之後藉由研究ERF19所結合的DNA序列將有助於深入瞭解ERF19在阿拉伯芥抵抗灰黴病菌的作用機制。 Botrytis cinerea is a necrotrophic fungal pathogen that attacks many crop plants. B. cinerea triggers plants to generate large amounts of reactive oxygen species (ROS) and induces local cell death to facilitate infection. Both jasmonic acid (JA) and ethylene (ET) signaling pathways play critical roles during Arabidopsis resistance to B. cinerea. Some ETHYLENE RESPONSE FACTORs (ERFs) are known to be involved in B. cinerea resistance. Through the screening of an over-expression transcription factors library, we identified AtERF19 as a critical player in Arabidopsis resistance to B. cinerea. ERF19 belongs to the DREB subfamily A-5 of ERF/AP2 transcription factor. Arabidopsis lines over-expressing ERF19 developed more severe necrosis symptoms than wild-type plants. Moreover, accumulation of the JA/ET-dependent plant defensin PDF1.2 was reduced in mock- and B. cinerea-inoculated ERF19 over-expression lines. In addition, a stronger ROS burst was observed in OE lines. Our findings suggest that not only the ROS burst but also JA/ET signaling pathways are probably associated with AtERF19-mediated signaling in response to B. cinerea attack. Further studies of AtERF19 binding sequences and target genes will help elucidate the role of the plant transcription factor ERF19 during Arabidopsis infection by B. cinerea. |
URI: | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/58546 |
全文授權: | 有償授權 |
顯示於系所單位: | 植物科學研究所 |
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