請用此 Handle URI 來引用此文件：
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/58377完整後設資料紀錄
| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 謝文陽(Wung-Yang Shieh) | |
| dc.contributor.author | Han-Yu Tsai | en |
| dc.contributor.author | 蔡涵聿 | zh_TW |
| dc.date.accessioned | 2021-06-16T08:13:04Z | - |
| dc.date.available | 2014-03-09 | |
| dc.date.copyright | 2014-03-09 | |
| dc.date.issued | 2014 | |
| dc.date.submitted | 2014-02-14 | |
| dc.identifier.citation | Annweiler, E., Materna, A., Safinowski, M., Kappler, A., Richnow, H. H., Michaelis, W., & Meckenstock, R. U. (2000). Anaerobic degradation of 2-methylnaphthalene by a sulfate-reducing enrichment culture. Applied and Environmental Microbiology, 66(12), 5329-5333.
ATSDR. (2005). Toxicological profile for Naphthalene, 1-Methylnaphthalene, and 2-Methylnaphthalene. ATSDR. (2009). Case studies in environmental medicine toxicity of polycyclic aromatic hydrocarbons (PAHs). Bamforth, S. M., & Singleton, I. (2005). Bioremediation of polycyclic aromatic hydrocarbons: current knowledge and future directions. Journal of Chemical Technology and Biotechnology, 80(7), 723-736. Chen, Q., Janssen, D. B., & Witholt, B. (1996). Physiological changes and alk gene instability in Pseudomonas oleovorans during induction and expression of alk genes. Journal of Bacteriology, 178(18), 5508-5512. Dean-Ross, D., Moody, J., & Cerniglia, C. E. (2002). Utilization of mixtures of polycyclic aromatic hydrocarbons by bacteria isolated from contaminated sediment. Fems Microbiology Ecology, 41(1), 1-7. Ferrero, M., Llobet-Brossa, E., Lalucat, J., Garcia-Valdes, E., Rossello-Mora, R., & Bosch, R. (2002). Coexistence of two distinct copies of naphthalene degradation genes in Pseudomonas strains isolated from the western Mediterranean region. Applied and Environmental Microbiology, 68(2), 957-962. Goris, J., Konstantinidis, K. T., Klappenbach, J. A., Coenye, T., Vandamme, P., & Tiedje, J. M. (2007). DNA-DNA hybridization values and their relationship to whole-genome sequence similarities. International Journal of Systematic and Evolutionary Microbiology, 57(Pt 1), 81-91. Janda, J. M., & Abbott, S. L. (2007). 16S rRNA gene sequencing for bacterial identification in the diagnostic laboratory: pluses, perils, and pitfalls. Journal of Clinical Microbiology, 45(9), 2761-2764. Kazunga, C., & Aitken, M. D. (2000). Products from the incomplete metabolism of pyrene by polycyclic aromatic hydrocarbon-degrading bacteria. Applied and Environmental Microbiology, 66(5), 1917-1922. Mahajan, M. C., Phale, P. S., & Vaidyanathan, C. S. (1994). Evidence for the involvement of multiple pathways in the biodegradation of 1- and 2-methylnaphthalene by Pseudomonas putida CSV86. Archives of Microbiology, 161(5), 425-433. Murata, Y., Emi, Y., Denda, A., & Konishi, Y. (1992). Ultrastructural analysis of pulmonary alveolar proteinosis induced by methylnaphthalene in mice. Experimental and Toxicologic Pathology, 44(1), 47-54. Palleroni, N. J., Doudoroff, M., Stanier, R. Y., Solanes, R. E., & Mandel, M. (1970). Taxonomy of the aerobic pseudomonads: the properties of the Pseudomonas stutzeri group. Journal of General Microbiology, 60(2), 215-231. Samanta, S. K., Chakraborti, A. K., & Jain, R. K. (1999). Degradation of phenanthrene by different bacteria: evidence for novel transformation sequences involving the formation of 1-naphthol. Applied Microbiology and Biotechnology 53(1), 98-107. Selesi, D., Jehmlich, N., von Bergen, M., Schmidt, F., Rattei, T., Tischler, P., Meckenstock, R. U. (2010). Combined genomic and proteomic approaches identify gene clusters involved in anaerobic 2-methylnaphthalene degradation in the sulfate-reducing enrichment culture N47. Journal of Bacteriology, 192(1), 295-306. Shimomura, Lisa. (2000). Determination of the presence of a naphthalene dioxygenase gene in microorganisms isolated from contaminated soils on the central coast of califormia. Biological Sciences Department. California Polytechnic State University. Stringfellow, W. T., & Aitken, M. D. (1995). Competitive metabolism of naphthalene, methylnaphthalenes, and fluorene by phenanthrene-degrading pseudomonads. Applied and Environmental Microbiology, 61(1), 357-362. USEPA. (1995). Cost and performance report, land treatment at the Scott Lumber company superfund site, Alton, Missouri. Verhille, S., Baida, N., Dabboussi, F., Hamze, M., Izard, D., & Leclerc, H. (1999). Pseudomonas gessardii sp. nov. and Pseudomonas migulae sp. nov., two new species isolated from natural mineral waters. International Journal of Systematic and Evolutionary Microbiology, 49 Pt 4, 1559-1572. Wakeham, S. G., Schaffner, C., & Giger, W. (1980). Polycyclic aromatic-hydrocarbons in recent lake-sediments. 2.compounds derived from biogenic precursors during early diagenesis. Geochimica Et Cosmochimica Acta, 44(3), 415-429. Warshawsky, D. (2001). Polycyclic and heterocyclic aromatic hydrocarbons Patty's Toxicology (5 ed., Vol. 4): John Wiley & Sons, Inc. Wei Zhou, Donglan He, Xiaohua Li, Huanhuan Zhang, Xiaobo Zeng & Guojun Cheng. (2013). Isolation and characterization of naphthalene-degrading strains, Pseudomonas sp. CZ2 and CZ5. African Journal of Microbiology Research, 7, 13-19. Weissenfels, W. D., Beyer, M., & Klein, J. (1990). Degradation of phenanthrene, fluorene and fluoranthene by pure bacterial cultures. Applied Microbiology and Biotechnology, 32(4), 479-484. Williams, P. A., Catterall, F. A., & Murray, K. (1975). Metabolism of naphthalene, 2-methylnaphthalene, salicylate, and benzoate by Pseudomonas PG: regulation of tangential pathways. Journal of Bacteriology, 124(2), 679-685. Wong, J. W. C., Lai, K. M., Wan, C. K., Ma, K. K., & Fang, M. (2002). Isolation and optimization of PAH-degradative bacteria from contaminated soil for PAHs bioremediation. Water Air and Soil Pollution, 139(1-4), 1-13. 楊金鐘 (1999). 汙染土壤之整治復育技術. 第五屆土壤汙染防治研討會論文集: 第47頁. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/58377 | - |
| dc.description.abstract | 多環芳香烴 (Polycyclic aromatic hydrocarbons, PAHs) 為近年來備受關注的汙染物，經由水、空氣或食物進入人體。許多多環芳香烴已經被認定為致癌物，臨床實驗報告指出若長期接觸高濃度多環芳香烴混合物，會導致皮膚癌、胃癌或肝癌的產生。研究發現很多細菌可以利用低分子量的PAHs作為單一碳源，將它們轉換為代謝路徑中較簡單受質。
微生物對PAHs的生物分解作用可分為礦化作用 (mineralization)、共代謝作用 (cometabolism) 及非專一性自由基氧化作用 (unspecific radical oxidation)。為了解台灣沿岸海域中具有分解二甲基萘 (2-methylnaphthalene) 能力的細菌種類、不同培養條件對菌株降解二甲基萘的影響、菌株降解二甲基萘的可能機制，本研究以多環芳香烴為單一碳源進行增菌培養，分離出四株可利用二甲基萘為單一碳源的細菌，經16S rRNA基因定序，三株為假單胞菌屬 (Pseudomonas) 及一株為 Cobetia菌屬，而假單胞菌屬中編號ME2具有良好降解活性。利用高效能液相層析儀 (High Performance Liquid Chromatography, HPLC) 探討ME2在不同培養條件下降解二甲基萘的差異，發現在單一碳源的情況下，ME2在pH8、30℃下，培養30天後可將1000 ppm二甲基萘降解 40~50%，而供給有機營養物可促使菌株降解達 70%。 | zh_TW |
| dc.description.abstract | Polycyclic aromatic hydrocarbons (PAHs) pollution has attracted much of attention in recent years. The pollutants can enter the human body through water, air or food. Many of the PAHs are carcinogens. They could cause skin cancer, stomach cancer or liver cancer when human expose to PAHs in high concentrations for a long period of time. It was found that many bacteria can use low molecular weight PAHs as single carbon source and convert them into simple substrates in the metabolic pathway. Microbial biodegradation of PAHs can be classified into mineralization, cometabolism and unspecific radical oxidation.
To investigate the bacterial species that have the ability to decompose 2-methylnaphthalene in the coastal waters of Taiwan, the influences of different culture conditions on the degradation of PAHs, and possible mechanisms of 2-methylnaphthalene degradation, the present study used 2-methylnaphthalene as sole carbon source in the enrichment culture. Four bacteria strains were isolated. Strains ME2, ME3 and ME4 are Pseudomonas spp. and strain ME1 is Cobetia sp. Strain ME2 has the best degradation activity. It was chosen for the later study. High performance liquid chromatography (HPLC) was used to investigate the bacterial PAHs degradation ability in different culture conditions. Strain ME2 could degrade 400~500 ppm 2-methylnaphthalene after 30 days at pH 8 and 30 ℃. When supplied with organic nutrients, the degradation ability of ME2 was enhanced up to 700 ppm. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-16T08:13:04Z (GMT). No. of bitstreams: 1 ntu-103-R00241214-1.pdf: 1016228 bytes, checksum: 4032136f7af59d4ec34e21b11045f56c (MD5) Previous issue date: 2014 | en |
| dc.description.tableofcontents | 中文摘要 …ii
Abstract ...iii 第一章 前言 ….1 第一節、 多環芳香烴簡介 1 第二節、 降解多環芳香烴之微生物 2 第三節、 多環芳香烴汙染實例 2 第四節、 多環芳香烴整治現況 3 第五節、 2-甲基萘及其毒性 3 第六節、 2-甲基萘降解途徑 4 第二章 材料和方法 ….5 第一節、 細菌樣本 5 第二節、 培養基配製 (成分比例詳見附錄一、二) 5 第三節、 分離、培養與純化菌株 5 第四節、 分解效率再現性之測試 6 第五節、 2-甲基萘與萘濃度檢量線製備 7 第六節、 2-甲基萘降解能力測試 8 第七節、 分離株形態與生理生化測試 11 第八節、 分離株之16S rRNA基因分析 16 第九節、 nahAc基因分析 20 第十節、 ndo基因分析 21 第三章 結果 ...22 第一節、 2-甲基萘降解菌株的篩選與分離 22 第二節、 2-甲基萘降解細菌分離株16S rRNA基因序列分析 22 第三節、 2-甲基萘降解細菌分離株16S rRNA基因的親緣分析 23 第四節、 2-甲基萘降解細菌分離株形態與生理生化測試 23 第五節、 分離株之2-甲基萘降解能力測試 25 第六節、 2-甲基萘降解相關基因探討 27 第四章 討論 ...28 第一節、 分離株之16S rRNA分析與生理生化特性比較 28 第二節、 分離株之2-甲基萘降解能力探討 31 第三節、 2-甲基萘降解性細菌探討 33 第五章 總結 ...34 Reference ...35 表目錄 表一、各分離株與最相似菌種的 16S rRNA 基因相似度 39 表二、各分離株在各種不同生長條件的測試 40 表三、各分離株的酵素活性生化反應測試 41 表四、各分離株利用不同單一碳源生長測試 42 表五、各分離株發酵測試結果 43 表六、分離株對於其他芳香烴作為單一碳源利用測試 44 表七、ME1分離株與標準菌株的比較 45 表八、ME2分離株與標準菌株的比較 46 表九、ME3分離株與標準菌株的比較 47 表十、ME4分離株與標準菌株的比較 48 表十一、不同培養基對ME1、ME3及ME4 菌株降解2-甲基萘的影響 49 圖目錄 圖一、歸類為 Cobetia屬的分離株及其相似菌種的標準菌株之 16S rRNA基因親緣演化樹 50 圖二、歸類為 Pseudomonas屬的分離株及其相似菌種的標準菌株之 16S rRNA基因親緣演化樹 51 圖三、歸類為 Pseudomonas屬的分離株及其相似菌種的標準菌株之 16S rRNA基因親緣演化樹 52 圖四、萘之 HPLC檢量線 53 圖五、2-甲基萘之 HPLC檢量線 53 圖六、不同溫度下菌株 ME2的2-甲基萘濃度變化曲線 54 圖七、不同溫度下菌株 ME2的生長曲線..................................................................54 圖八、不同起始濃度下菌株 ME2的2-甲基萘濃度變化曲線 55 圖九、不同起始濃度下菌株 ME2的生長曲線 55 圖十、不同 pH下菌株 ME2的2-甲基萘濃度變化曲線 56 圖十一、不同 pH下菌株 ME2的生長曲線 56 圖十二、不同培養基下菌株 ME2的2-甲基萘濃度變化曲線 57 圖十三、不同培養基下菌株 ME2的生長曲線 57 圖十四、添加2-甲基萘與否之菌株 ME2在 PY培養基的生長曲線 58 附錄目錄 附錄一、PY培養基、 PY培養液、1/10 PY培養液、 PY保存培養基成分 59 附錄二、PYC培養基、 M培養液成分 60 附錄三、2-甲基萘結構式 61 | |
| dc.language.iso | zh-TW | |
| dc.subject | 高效能液相層析儀 | zh_TW |
| dc.subject | Pseudomonas | zh_TW |
| dc.subject | Cobetia | zh_TW |
| dc.subject | 培養條件 | zh_TW |
| dc.subject | 二甲基?降解 | zh_TW |
| dc.subject | Pseudomonas | en |
| dc.subject | Cobetia | en |
| dc.subject | HPLC | en |
| dc.subject | 2-methylnaphthalene | en |
| dc.title | 臺灣北部沿海中2-甲基萘降解細菌的分離及特性研究 | zh_TW |
| dc.title | Isolation and characterization of 2-methylnaphthalene-degrading marine bacteria from the coastal water of northern Taiwan | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 102-1 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 劉玫英(Mei-Ying Liu),李宗徽(Tzung-Huei Li),陳卓昇(Juo-Sheng chen),劉秀美(Shiou-Mei Liu) | |
| dc.subject.keyword | 二甲基?降解,培養條件,高效能液相層析儀,Pseudomonas,Cobetia, | zh_TW |
| dc.subject.keyword | Pseudomonas,Cobetia,HPLC,2-methylnaphthalene, | en |
| dc.relation.page | 61 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2014-02-14 | |
| dc.contributor.author-college | 理學院 | zh_TW |
| dc.contributor.author-dept | 海洋研究所 | zh_TW |
| 顯示於系所單位： | 海洋研究所 | |
文件中的檔案：
| 檔案 | 大小 | 格式 | |
|---|---|---|---|
| ntu-103-1.pdf 未授權公開取用 | 992.41 kB | Adobe PDF |
系統中的文件，除了特別指名其著作權條款之外，均受到著作權保護，並且保留所有的權利。