請用此 Handle URI 來引用此文件:
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/58082
完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 賀端華(Tuan-hua David Ho) | |
dc.contributor.author | Ching-Yi Liao | en |
dc.contributor.author | 廖靜誼 | zh_TW |
dc.date.accessioned | 2021-06-16T08:05:38Z | - |
dc.date.available | 2019-06-30 | |
dc.date.copyright | 2014-07-08 | |
dc.date.issued | 2014 | |
dc.date.submitted | 2014-06-25 | |
dc.identifier.citation | Adamski, N.M., Anastasiou, E., Eriksson, S., O'Neill, C.M., and Lenhard, M. (2009). Local maternal control of seed size by KLUH/CYP78A5-dependent growth signaling. Proc Natl Acad Sci U S A 106: 20115-20120.
Anastasiou, E., Kenz, S., Gerstung, M., MacLean, D., Timmer, J., Fleck, C., and Lenhard, M. (2007). Control of plant organ size by KLUH/CYP78A5-dependent intercellular signaling. Dev Cell 13: 843-856. Ashikari, M., Sakakibara, H., Lin, S., Yamamoto, T., Takashi, T., Nishimura, A., Angeles, E.R., Qian, Q., Kitano, H., and Matsuoka, M. (2005). Cytokinin oxidase regulates rice grain production. Science 309: 741-745. Bommert, P., Nagasawa, N.S., and Jackson, D. (2013). Quantitative variation in maize kernel row number is controlled by the FASCIATED EAR2 locus. Nat Genet 45: 334-337. Chen, Y., Fan, X., Song, W., Zhang, Y., and Xu, G. (2012). Over-expression of OsPIN2 leads to increased tiller numbers, angle and shorter plant height through suppression of OsLAZY1. Plant Biotechnol J 10: 139-149. Chern, C.G., Fan, M.J., Yu, S.M., Hour, A.L., Lu, P.C., Lin, Y.C., Wei, F.J., Huang, S.C., Chen, S., Lai, M.H., Tseng, C.S., Yen, H.M., Jwo, W.S., Wu, C.C., Yang, T.L., Li, L.S., Kuo, Y.C., Li, S.M., Li, C.P., Wey, C.K., Trisiriroj, A., Lee, H.F., and Hsing, Y.I. (2007). A rice phenomics study--phenotype scoring and seed propagation of a T-DNA insertion-induced rice mutant population. Plant Mol Biol 65: 427-438. Clouse, S.D. (2011). Brassinosteroid signal transduction: from receptor kinase activation to transcriptional networks regulating plant development. Plant Cell 23: 1219-1230. Donald, C.M.t. (1968). The breeding of crop ideotypes. Euphytica 17: 385-403. Du, L., Li, N., Chen, L., Xu, Y., Li, Y., Zhang, Y., Li, C., and Li, Y. (2014). The ubiquitin receptor DA1 regulates seed and organ size by modulating the stability of the ubiquitin-specific protease UBP15/SOD2 in Arabidopsis. Plant Cell 26: 665-677. Duan, P., Rao, Y., Zeng, D., Yang, Y., Xu, R., Zhang, B., Dong, G., Qian, Q., and Li, Y. (2014). SMALL GRAIN 1, which encodes a mitogen-activated protein kinase kinase 4, influences grain size in rice. Plant J 77: 547-557. Fan, C., Xing, Y., Mao, H., Lu, T., Han, B., Xu, C., Li, X., and Zhang, Q. (2006). GS3, a major QTL for grain length and weight and minor QTL for grain width and thickness in rice, encodes a putative transmembrane protein. Theor Appl Genet 112: 1164-1171. Fang, W., Wang, Z., Cui, R., Li, J., and Li, Y. (2012). Maternal control of seed size by EOD3/CYP78A6 in Arabidopsis thaliana. Plant J 70: 929-939. Fuller, D.Q. (2007). Contrasting patterns in crop domestication and domestication rates: recent archaeobotanical insights from the Old World. Ann Bot 100: 903-924. Garcia, D., Fitz Gerald, J.N., and Berger, F. (2005). Maternal control of integument cell elongation and zygotic control of endosperm growth are coordinated to determine seed size in Arabidopsis. Plant Cell 17: 52-60. Guo, H., Li, L., Aluru, M., Aluru, S., and Yin, Y. (2013). Mechanisms and networks for brassinosteroid regulated gene expression. Curr Opin Plant Biol 16: 545-553. Horiguchi, G., Ferjani, A., Fujikura, U., and Tsukaya, H. (2006). Coordination of cell proliferation and cell expansion in the control of leaf size in Arabidopsis thaliana. J Plant Res 119: 37-42. Hu, X., Qian, Q., Xu, T., Zhang, Y., Dong, G., Gao, T., Xie, Q., and Xue, Y. (2013). The U-box E3 ubiquitin ligase TUD1 functions with a heterotrimeric G alpha subunit to regulate Brassinosteroid-mediated growth in rice. PLoS Genet 9: e1003391. Hu, Y., Xie, Q., and Chua, N.H. (2003). The Arabidopsis auxin-inducible gene ARGOS controls lateral organ size. Plant Cell 15: 1951-1961. Huang, J., Pray, C., and Rozelle, S. (2002). Enhancing the crops to feed the poor. Nature 418: 678-684. Huang, R., Jiang, L., Zheng, J., Wang, T., Wang, H., Huang, Y., and Hong, Z. (2013). Genetic bases of rice grain shape: so many genes, so little known. Trends Plant Sci 18: 218-226. Huang, X., Qian, Q., Liu, Z., Sun, H., He, S., Luo, D., Xia, G., Chu, C., Li, J., and Fu, X. (2009). Natural variation at the DEP1 locus enhances grain yield in rice. Nat Genet 41: 494-497. Ikeda, M., Miura, K., Aya, K., Kitano, H., and Matsuoka, M. (2013). Genes offering the potential for designing yield-related traits in rice. Curr Opin Plant Biol 16: 213-220. Inze, D., and De Veylder, L. (2006). Cell cycle regulation in plant development. Annu Rev Genet 40: 77-105. Ishimaru, K., Hirotsu, N., Madoka, Y., Murakami, N., Hara, N., Onodera, H., Kashiwagi, T., Ujiie, K., Shimizu, B., Onishi, A., Miyagawa, H., and Katoh, E. (2013). Loss of function of the IAA-glucose hydrolase gene TGW6 enhances rice grain weight and increases yield. Nat Genet 45: 707-711. Jain, M., Kaur, N., Tyagi, A.K., and Khurana, J.P. (2006). The auxin-responsive GH3 gene family in rice (Oryza sativa). Funct Integr Genomics 6: 36-46. Jiang, Y., Bao, L., Jeong, S.Y., Kim, S.K., Xu, C., Li, X., and Zhang, Q. (2012). XIAO is involved in the control of organ size by contributing to the regulation of signaling and homeostasis of brassinosteroids and cell cycling in rice. Plant J 70: 398-408. Jiao, Y., Wang, Y., Xue, D., Wang, J., Yan, M., Liu, G., Dong, G., Zeng, D., Lu, Z., Zhu, X., Qian, Q., and Li, J. (2010). Regulation of OsSPL14 by OsmiR156 defines ideal plant architecture in rice. Nat Genet 42: 541-544. Jofuku, K.D., Omidyar, P.K., Gee, Z., and Okamuro, J.K. (2005). Control of seed mass and seed yield by the floral homeotic gene APETALA2. Proc Natl Acad Sci U S A 102: 3117-3122. Kang, X., Li, W., Zhou, Y., and Ni, M. (2013). A WRKY transcription factor recruits the SYG1-like protein SHB1 to activate gene expression and seed cavity enlargement. PLoS Genet 9: e1003347. Kim, T.W., and Wang, Z.Y. (2010). Brassinosteroid signal transduction from receptor kinases to transcription factors. Annu Rev Plant Biol 61: 681-704. Kurakawa, T., Ueda, N., Maekawa, M., Kobayashi, K., Kojima, M., Nagato, Y., Sakakibara, H., and Kyozuka, J. (2007). Direct control of shoot meristem activity by a cytokinin-activating enzyme. Nature 445: 652-655. Li, J., Nie, X., Tan, J.L., and Berger, F. (2013). Integration of epigenetic and genetic controls of seed size by cytokinin in Arabidopsis. Proc Natl Acad Sci U S A 110: 15479-15484. Li, M., Tang, D., Wang, K., Wu, X., Lu, L., Yu, H., Gu, M., Yan, C., and Cheng, Z. (2011). Mutations in the F-box gene LARGER PANICLE improve the panicle architecture and enhance the grain yield in rice. Plant Biotechnol J 9: 1002-1013. Li, X., Qian, Q., Fu, Z., Wang, Y., Xiong, G., Zeng, D., Wang, X., Liu, X., Teng, S., Hiroshi, F., Yuan, M., Luo, D., Han, B., and Li, J. (2003). Control of tillering in rice. Nature 422: 618-621. Li, Y., Zheng, L., Corke, F., Smith, C., and Bevan, M.W. (2008). Control of final seed and organ size by the DA1 gene family in Arabidopsis thaliana. Genes Dev 22: 1331-1336. Mao, H., Sun, S., Yao, J., Wang, C., Yu, S., Xu, C., Li, X., and Zhang, Q. (2010). Linking differential domain functions of the GS3 protein to natural variation of grain size in rice. Proc Natl Acad Sci U S A 107: 19579-19584. Miura, K., Ikeda, M., Matsubara, A., Song, X.J., Ito, M., Asano, K., Matsuoka, M., Kitano, H., and Ashikari, M. (2010). OsSPL14 promotes panicle branching and higher grain productivity in rice. Nat Genet 42: 545-549. Nakagawa, H., Tanaka, A., Tanabata, T., Ohtake, M., Fujioka, S., Nakamura, H., Ichikawa, H., and Mori, M. (2012). Short grain1 decreases organ elongation and brassinosteroid response in rice. Plant Physiol 158: 1208-1219. Pautler, M., Tanaka, W., Hirano, H.Y., and Jackson, D. (2013). Grass meristems I: shoot apical meristem maintenance, axillary meristem determinacy and the floral transition. Plant Cell Physiol 54: 302-312. Piao, R., Jiang, W., Ham, T.H., Choi, M.S., Qiao, Y., Chu, S.H., Park, J.H., Woo, M.O., Jin, Z., An, G., Lee, J., and Koh, H.J. (2009). Map-based cloning of the ERECT PANICLE 3 gene in rice. Theor Appl Genet 119: 1497-1506. Qi, P., Lin, Y.S., Song, X.J., Shen, J.B., Huang, W., Shan, J.X., Zhu, M.Z., Jiang, L., Gao, J.P., and Lin, H.X. (2012). The novel quantitative trait locus GL3.1 controls rice grain size and yield by regulating Cyclin-T1;3. Cell Res 22: 1666-1680. Sauter, M., Mekhedov, S.L., and Kende, H. (1995). Gibberellin promotes histone H1 kinase activity and the expression of cdc2 and cyclin genes during the induction of rapid growth in deepwater rice internodes. Plant J 7: 623-632. Schruff, M.C., Spielman, M., Tiwari, S., Adams, S., Fenby, N., and Scott, R.J. (2006). The AUXIN RESPONSE FACTOR 2 gene of Arabidopsis links auxin signalling, cell division, and the size of seeds and other organs. Development 133: 251-261. Shomura, A., Izawa, T., Ebana, K., Ebitani, T., Kanegae, H., Konishi, S., and Yano, M. (2008). Deletion in a gene associated with grain size increased yields during rice domestication. Nat Genet 40: 1023-1028. Song, X.J., Huang, W., Shi, M., Zhu, M.Z., and Lin, H.X. (2007). A QTL for rice grain width and weight encodes a previously unknown RING-type E3 ubiquitin ligase. Nat Genet 39: 623-630. Stahl, Y., and Simon, R. (2010). Plant primary meristems: shared functions and regulatory mechanisms. Curr Opin Plant Biol 13: 53-58. Tanaka, A., Nakagawa, H., Tomita, C., Shimatani, Z., Ohtake, M., Nomura, T., Jiang, C.J., Dubouzet, J.G., Kikuchi, S., Sekimoto, H., Yokota, T., Asami, T., Kamakura, T., and Mori, M. (2009). BRASSINOSTEROID UPREGULATED1, encoding a helix-loop-helix protein, is a novel gene involved in brassinosteroid signaling and controls bending of the lamina joint in rice. Plant Physiol 151: 669-680. Tong, H., Liu, L., Jin, Y., Du, L., Yin, Y., Qian, Q., Zhu, L., and Chu, C. (2012). DWARF AND LOW-TILLERING acts as a direct downstream target of a GSK3/SHAGGY-like kinase to mediate brassinosteroid responses in rice. Plant Cell 24: 2562-2577. Tong, H., Jin, Y., Liu, W., Li, F., Fang, J., Yin, Y., Qian, Q., Zhu, L., and Chu, C. (2009). DWARF AND LOW-TILLERING, a new member of the GRAS family, plays positive roles in brassinosteroid signaling in rice. Plant J 58: 803-816. Vriet, C., Russinova, E., and Reuzeau, C. (2012). Boosting crop yields with plant steroids. Plant Cell 24: 842-857. Wang, E., Wang, J., Zhu, X., Hao, W., Wang, L., Li, Q., Zhang, L., He, W., Lu, B., Lin, H., Ma, H., Zhang, G., and He, Z. (2008). Control of rice grain-filling and yield by a gene with a potential signature of domestication. Nat Genet 40: 1370-1374. Wang, Y., and Li, J. (2006). Genes controlling plant architecture. Curr Opin Biotechnol 17: 123-129. Wu, C.Y., Trieu, A., Radhakrishnan, P., Kwok, S.F., Harris, S., Zhang, K., Wang, J., Wan, J., Zhai, H., Takatsuto, S., Matsumoto, S., Fujioka, S., Feldmann, K.A., and Pennell, R.I. (2008). Brassinosteroids regulate grain filling in rice. Plant Cell 20: 2130-2145. Xia, T., Li, N., Dumenil, J., Li, J., Kamenski, A., Bevan, M.W., Gao, F., and Li, Y. (2013). The ubiquitin receptor DA1 interacts with the E3 ubiquitin ligase DA2 to regulate seed and organ size in Arabidopsis. Plant Cell 25: 3347-3359. Xing, Y., and Zhang, Q. (2010). Genetic and molecular bases of rice yield. Annu Rev Plant Biol 61: 421-442. Ye, H., Li, L., and Yin, Y. (2011). Recent advances in the regulation of brassinosteroid signaling and biosynthesis pathways. J Integr Plant Biol 53: 455-468. Zhang, C., Bai, M.Y., and Chong, K. (2014). Brassinosteroid-mediated regulation of agronomic traits in rice. Plant Cell Rep 33: 683-696. Zhou, Y., Zhang, X., Kang, X., Zhao, X., Zhang, X., and Ni, M. (2009). SHORT HYPOCOTYL UNDER BLUE1 associates with MINISEED3 and HAIKU2 promoters in vivo to regulate Arabidopsis seed development. Plant Cell 21: 106-117. Zhu, K., Tang, D., Yan, C., Chi, Z., Yu, H., Chen, J., Liang, J., Gu, M., and Cheng, Z. (2010). Erect panicle2 encodes a novel protein that regulates panicle erectness in indica rice. Genetics 184: 343-350. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/58082 | - |
dc.description.abstract | 世界作物產率即將達到飽和,因應世界人口增加的食物需求,高產量作物的育種日漸重要。植物基因工程提供一個有效提高作物產量的途徑。利用正向遺傳學的方法,我們從台灣水稻T-DNA突變庫找到一個表現出大穀粒性狀的T-DNA活化表現株BG2act。插入點上下游有數個基因被T-DNA上的強化子活化表現。BIG GRAIN 2 (BG2)轉譯一個新穎的植物特有未知蛋白,胺基酸序列在開花植物裡具有保守性,確認其活化表現與大穀粒性狀有關。以玉米ubiquitin啟動子大量表現BG2,轉殖株呈現多效性的影響,包含植株高度、花穗長度、穀粒大小與分糵角度的增加。產量分析結果顯示,大量表現BG2亦增加了穀粒重量,總產量卻輕微減少,指出到處表現BG2對於增產有些負面影響。組織學分析顯示,BG2大量表現呈現的大穀粒是因為總細胞數目的增加而非細胞大小改變的影響,指出BG2可能參與細胞週期的調控。降低BG2表現的植株性狀近似於野生型,顯示BG2具有功能重複性。BG2廣泛表現於各發育時期,尤其在地上部年輕組織中表現最多。次細胞內定位分析結果顯示,BG2主要表現在近細胞膜的位置,在細胞質與細胞核內也有較低量表現。BG2的表現可被生長素、油菜素類固醇及激勃素誘導表現。生長素及油菜素類固醇生合成與訊息傳導的代表性基因在BG2大量表現植株中有增加與降低表現,幾個細胞週期調控基因也有表現增加情形。大量表現油菜素類固醇生合成訊息傳導途徑中正向調控子常引起植株高度與穀粒大小的增加,綜合以上結果我們發現BG2可能是一個新的油菜素類固醇訊息傳導途徑之正向調控子,透過促進細胞增殖控制水稻穀粒大小。BG2是否透過油菜素類固醇訊息傳導控制細胞週期以及是否涉及多種植物荷爾蒙交互條控仍待進一步的實驗證明。BG2的研究連結了油菜素類固醇等植物荷爾蒙與細胞週期對穀粒大小的調控,並可能成為一個新的分子育種標的基因。 | zh_TW |
dc.description.abstract | The world’s crop productivity has reached a plateau in recent years. Breeding of high-yielding rice is crucial for meeting the food demand of increasing world population. Plant genetic engineering offers an efficient alternative for increase in crop productivity. Using the forward genetic approach, a T-DNA activation tagged mutant BG2act exhibiting big grain phenotype was identified from our T-DNA the Taiwan Rice Insertional Mutagenesis (TRIM) population. Several genes flanking the T-DNA insertion site are activated by the enhancer in the T-DNA construct. BIG GRAIN 2 (BG2), a novel gene encodes an unknown function protein which is conserved in Angiosperms, is validated responsible for the big grain phenotype. Overexpression of BG2 by maize ubiquitin promoter causes pleiotropic effects in transgenic rice, including increase in plant height, panicle length, grain size, and tiller angle. Yield analysis of BG2OX shows increase in grain weight but slightly decrease in total yield, indicating other negative influences caused by ubiquitous expression of BG2. Histological analysis results shows that cell number but not cell size accounts for the increase in grain length in BG2OX and BG2act lines, indicating that BG2 may be involved in the cell cycle regulation in grain size regulation. Knockdown expression of BG2 displays similar phenotype as wild-type plants, suggesting that BG2 has functional redundancy. BG2 is expressed ubiquitously during rice development, especially in shoot and young tissues. BG2 is mainly localized in plasma membrane and connected with the cell wall, and also localized in cytoplasm and nucleus at lower level. Expression of BG2 can be induced by auxin, brassinosteroids (BRs), and gibberellin (GA). Genes involved in biosynthesis and signaling of auxin and BR are up-regulated or down-regulated in BG2OX transgenic plant. Several genes involved in cell cycle also detected up-regulated. As overexpression of BRs biosynthesis genes and positive regulators usually lead to increase of plant height and grain size, these results indicate that BG2 may act as a novel positive regulator of BR response, increasing grain size by promoting cell proliferation. Crosstalk of hormones and other grain size control genes in grain size regulation will be further demonstrated. This study suggests that BG2 may provide a possible connection between plant hormones such as BRs and cell cycle regulation in grain size control, potentially as the target applied for molecular breeding. | en |
dc.description.provenance | Made available in DSpace on 2021-06-16T08:05:38Z (GMT). No. of bitstreams: 1 ntu-103-R01b42011-1.pdf: 9218686 bytes, checksum: 2d771e9681be8eb83170e7ad91cdef5e (MD5) Previous issue date: 2014 | en |
dc.description.tableofcontents | 口試委員會審定書 i
謝誌 ii 中文摘要 iii Abstract v Content of Figures x Content of Tables xi Chapter 1 Introduction 1 1.1 Food crisis and crop productivity 1 1.2 Rice functional genomics for yield improvement 1 1.3 Factors controlling yield-related traits in rice 3 1.3.1 Plant architecture 3 1.3.2 Panicle morphology and grain number 3 1.3.3 Grain size and filling 4 1.4 Brassinosteroids and yield improvement in rice 6 1.4.1 Brassinosteroid signaling transduction 6 1.4.2 Yield improvement by brassinosteoids 8 1.5 Organ size control in plant 9 1.5.1 Cell cycle and plant hormone regulation 9 1.5.2 WUSHEL-CLV signaling and plant G protein network 10 1.5.3 Seed size control in Arabidopsis 11 1.5.4 Ubiquitin-proteasome pathway in seed size control 12 1.6 Specific aim and strategy 14 Chapter 2 Materials and Methods 15 2.1 Plant materials 15 2.2 Plasmid construction 15 2.3 Phylogenetic analysis of BG2-related protein 16 2.4 Gene bombardment and confocol microscopy 16 2.4.1 Midi preparation of plasmid DNA for transient subcellular localization assays 16 2.4.2 Particle bombardment 17 2.4.3 Confocol microscopy 18 2.5 RNA extraction 19 2.6 Reverse transcription polymerase chain reaction (RT-PCR) 20 2.7 Histochemical GUS staining assay 20 2.8 Scanning electronic microscope analysis 21 2.9 Hormone treatment 21 2.10 Antibody production 22 Chapter 3 Results 23 3.1 BG2 encodes an unknown function protein and is conserved among Angiosperms. 23 3.2 Characterization of the BG2 overexpression lines 25 3.3 Histological analysis of BG2OX lines 26 3.4 Expression profile of BG2 27 3.4.1 BG2 is preferentially expressed in the developing tissues. 27 3.4.2 BG2 located majorly in cell membrane. 28 3.6 Mechanism of organ size control 29 3.6.1 BG2 overexpression up-regulates expression of cell cycle regulating genes. 29 3.6.2 BG2 expression can be induced by auxin, GA, and BR. 30 3.6.3 BG2 overexpression up-regulates expression of genes involved in BRs biosynthesis and signaling. 30 3.6.4 BG2 overexpression up-regulates expression of genes involved in auxin biosynthesis and signaling. 31 3.6.5 BG2 overexpression up-regulates expression of yield trait relative genes. 31 Chapter 4 Discussion 33 4.1 Pleiotropic effects are an important consideration in molecular breeding programs 33 4.2 Functional redundancy between BG2 and BG2L 34 4.3 The roles BG2 plays in the BR signal transduction pathway 34 4.4 Putative function linkage between BRs and cell cycle in grain size control 36 4.5 Hormone crosstalk among BRs, auxin and GAs on organ size control 37 4.6 Yield control genes and G protein network 39 4.7 Grain size control genes and domestication of crops 39 4.8 BG2 application to enhance rice yield 41 4.9 Conclusion 42 4.10 Future works 43 References 46 Figures 56 Tables 82 Appendix 88 | |
dc.language.iso | en | |
dc.title | 一個新穎的植物特有基因BIG GRAIN 2透過促進細胞增殖調控水稻穀粒大小 | zh_TW |
dc.title | BIG GRAIN 2, a novel plant specific gene involved in promoting cell proliferation to control grain size in rice | en |
dc.type | Thesis | |
dc.date.schoolyear | 102-2 | |
dc.description.degree | 碩士 | |
dc.contributor.coadvisor | 余淑美(Su-May Yu) | |
dc.contributor.oralexamcommittee | 張英?(Ing-Feng Chang) | |
dc.subject.keyword | 水稻,產量相關性狀,器官大小,細胞增長,植物荷爾蒙,生長素,油菜素類固醇,激勃素, | zh_TW |
dc.subject.keyword | Oryza sativa,yield-related trait,organ size,cell proliferation,plant hormone,auxin,brassinosteroids,gibberellin, | en |
dc.relation.page | 93 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2014-06-25 | |
dc.contributor.author-college | 生命科學院 | zh_TW |
dc.contributor.author-dept | 植物科學研究所 | zh_TW |
顯示於系所單位: | 植物科學研究所 |
文件中的檔案:
檔案 | 大小 | 格式 | |
---|---|---|---|
ntu-103-1.pdf 目前未授權公開取用 | 9 MB | Adobe PDF |
系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。