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標題: | 蠶絲蛋白/肌腱胞外基質支架於肌腱修復上之應用 Application of Silk Fibroin/Tendon-Derived Extracellular Matrix Scaffold for Tendon Repair |
作者: | Jia-Ni Lee 李佳霓 |
指導教授: | 黃義侑(Yi-You Huang) |
關鍵字: | 肌腱組織工程,氣隙電紡絲法,蠶絲蛋白,細胞外基質,肌腱修復, Tendon tissue engineering,Air-gap electrospun,Silk fibroin,Extracellular matrix,Tendon repair, |
出版年 : | 2014 |
學位: | 碩士 |
摘要: | 肌腱組織由緻密的膠原纖維束所組成,主要功能為負責關節活動過程中之力量傳遞。目前臨床之腱傷可透過自體移植(autografts)、異體移植(allografts)、異種移植(xenografts)或人工肌腱置換治療。然而,提供移植處產生併發症、異體移植引發免疫排斥與人工肌腱疲乏等潛在問題,均促使仿生肌腱修復基材之發展。
過往文獻指出肌腱經去細胞處理後將可保留大量的胞外基質與相關生長因子,有鑑於此,我們將電紡所得之蠶絲蛋白纖維束浸泡於肌腱胞外基質液中,後續凍乾處理得結構與微環境近似天然組織之蠶絲/肌腱胞外基質(SF/t-ECM)複合支架。 將材料與肌腱細胞(tenocytes)共同培養七天後,SF/t-ECM複合支架細胞增生約60 %並開始分泌膠原蛋白;SEM及Confocal則可觀察到細胞於SF/t-ECM複合支架表層及深部細胞成紡錘狀貼附良好;另一方面,HE染色顯示SF/t-ECM複合支架於培養十六天後細胞已可浸潤到底層纖維,同時間點下SF支架則未觀察到此現象;動物實驗的部份,材料植入後十六天HE及Masson’s Trichrome染色均顯示SF/t-ECM複合支架相較SF之組別有更多的纖維母細胞(fibroblasts)浸潤及膠原纖維(collagen fibers)生成。 本研究設計之SF/t-ECM複合支架,解決了現階段肌腱組織工程上細胞無法深部浸潤支架增生並分泌胞外基質來提供機械強度之問題,結果顯示此近似天然組織之仿生基材未來應於肌腱修復上將相當具有潛力。 Tendons, which play an important role in force transmission, are composed of densely packed collagen fibers. When tendons are injured, surgical treatments are used to repair the damaged tendon by autografts, allografts, xenografts, or prosthetic devices. However, the risks of donor-site morbidity, transplant rejection, and injury recurrence usually limit long-term function recovery. There is a need to engineer biomaterial scaffolds that mimic the native tendon to overcome these problems. Previous studies have shown a lot of tendon extracellular matrix components as well as growth factors are still retained in decellularized tissues. Hence, we developed the silk fibroin/tendon-derived extracellular matrix (SF/t-ECM) scaffolds that are to be morphologically, biochemically, and histologically similar to the ECM of native tendon tissues. The results indicate cell proliferation on the SF/t-ECM scaffolds was significantly higher than that on the silk fibroin (SF) control groups after 7 days of cell culture. Histology demonstrated cells infiltrated through the full thickness of the SF/t-ECM scaffolds. Moreover, in rat tendon repair model, the SF/t-ECM scaffolds exhibited more fibroblasts proliferation and collagen fibers synthesis as compared with the SF control groups after 16 days of scaffold implanted. This investigation demonstrate that SF/t-ECM scaffolds have potential application in tendon repair. |
URI: | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/57969 |
全文授權: | 有償授權 |
顯示於系所單位: | 醫學工程學研究所 |
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