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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 林江珍 | |
dc.contributor.author | Chieh-Ling Chen | en |
dc.contributor.author | 陳玨伶 | zh_TW |
dc.date.accessioned | 2021-06-16T07:06:10Z | - |
dc.date.available | 2019-08-11 | |
dc.date.copyright | 2014-08-11 | |
dc.date.issued | 2014 | |
dc.date.submitted | 2014-07-10 | |
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dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/57830 | - |
dc.description.abstract | 近幾年,奈米複合材料廣泛於生物科技領域發展,例如:奈米銀粒子或是奈米磁性鐵粒子固定於脫層矽片上。脫層矽片包含奈米矽片(NSP)或是奈米雲母片(NMP)。奈米矽片經由蒙托土脫層而奈米雲母片經由氟化雲母脫層。奈米矽片的片徑為80-100奈米,而奈米雲母片為300-1000奈米,但兩者厚度皆為1奈米。NSP或是NMP具有物理貼附於細菌表面的特性,因此奈米金屬粒子/奈米雲母片(NMP)可以有不同之應用。
第一部份,將奈米銀粒子固定於奈米雲母片(NMP)之奈米複合材料可藉由表面增強拉曼散射(SERS)方式來偵測細菌的光譜訊號。在表面增強拉曼散射之偵測技術上,奈米銀粒子/奈米雲母片具有可撓性與三維熱點效應(尤其是z方向),可以增加SERS偵測的靈敏度與穩定性。由於細菌的細胞壁化學成分有所差別,例如革蘭氏陽性菌(金黃色葡萄菌)及革蘭氏陰性菌(大腸桿菌),SERS技術可以很快速地將其辨別出來。 第二部分,我們發現NMP具有非常優異的細菌捕捉能力。因此,我們利用磁性奈米粒子容易分離的特性,合成新穎的奈米磁性鐵粒子/奈米雲母片用於捕捉水中的細菌。在NMP水溶液中以原位(in situ)聚合方式,共沉澱二價及三價鐵離子鹽類,將使奈米磁性鐵粒子(Fe3O4)緊密附著於NMP表面,奈米磁性粒子粒徑大小約為8.3奈米。NMP-Fe3O4奈米複合物可以在水中捕捉且將細菌聚集成團,並藉由磁鐵可將捕捉之細菌分離出來。將NMP-Fe3O4奈米複合材料加入104 CFU/mL金黃色葡萄球菌之菌液,經由磁鐵分離後可使菌量大幅下降至100 CFU/mL。由掃描式電子顯微鏡(SEM)可以觀察NMP與細菌表面緊密的貼附。因此,此新穎的磁性Fe3O4/NMP奈米複合材料將可輕易且快速地將水中之細菌分離。 | zh_TW |
dc.description.abstract | Nanohybrids have been widely developed for biotechnology uses in recent years. Various nanohybrids are synthesized by embedded silver nanoparticles (AgNP) or iron oxide nanoparticles (Fe3O4) on the exfoliated silicate clay nanoplatelets. The exfoliated silicate clay nanoplatelets include nanoscale silicate platelets (NSP) from Montmorillonite and nanoscale Mica platelets (NMP) from the fluorinated Mica, which exhibit different lateral dimension of 80-100 nm for the NSP and 300-1000 nm for the NMP with the same thickness of 1 nm. The NSP or NMP nanohybrids are enabling of physically adhering onto to bacterial surfaces and resulting different applications, such as bacterial separation and detection.
In the first part of this study, novel nanohybrids of silver nanoparticles (AgNP) on the nanomica paltelets (NMP) can provide label-free analysis of bacteria via surface-enhanced Raman spectroscopy (SERS). The AgNP/NMP with flexibility and three-dimensional (3D) hotjunctions (particularly in z-direction) were discovered for improving the stability and enhancing of the sensitivity of the SERS nanotechnology. The SERS profiles recorded by such a platform are sensitive and stable, that could readily reflect different bacterial cell walls found in gram positive (S. aureus) and gram negative bacteria (E. coli). We further observed the unexpected bacterial capturing behavior for NMP, prepared from the exfoliation of layered structure of Mica clays. The new class of the nanohybrids comprising of the structurally exfoliated nanoscale Mica platelets (NMP) and magnetic iron-oxide nanoparticles (Fe3O4) were synthesized and employed for capturing bacteria in water. The in situ co-precipitation of aqueous Fe2+/Fe3+ salts subsequently afforded Fe3O4 of ca. 8.3 nm in diameter, tightly attached NMP surface. It was demonstrated that the NMP-Fe3O4 nanohybrids enabled to capture and aggregate the bacteria in water into lumps that became maneuverable and removable by a magnet. A single clycle of the nanohybrids treatment and subsequent magnetic removal was shown the reduction of the bacteria (Staphylococcus aureus, SA) from the concentration of 104 to 100 CFU/mL. The intensive adhering force of NMP and bacterial surface was also observable by using scanning electron microscope (SEM). The magnetically maneuvering the microbes through the association with the Fe3O4/NMP implies the potential design of new devices enabling the removal of microbes from water medium. | en |
dc.description.provenance | Made available in DSpace on 2021-06-16T07:06:10Z (GMT). No. of bitstreams: 1 ntu-103-R01549018-1.pdf: 3667630 bytes, checksum: a1da4082b24644e3b5f418425b9f592a (MD5) Previous issue date: 2014 | en |
dc.description.tableofcontents | 口試委員會審定書 …………………………………………………………................I
Acknowledgements ………………………………………………………….............. II 中文摘要 …………………………………………………………..............III Abstract ………………………………………………………...................V Contents ………………..…………………………………......................VII Figure captions …………………………………………………………..............IX Table captions ………………………………………………………...............XIV Scheme captions ………………………………………………………................XV Chapter 1 Introduction and Literature Review……........................................................1 1.1 Introduction of Nanomaterial……………………………………. 1 1.1.1. Background…….………………………….……...…….1 1.1.2. Nano Mica Platelets (NMP)………….…………………3 1.1.3. Silver Nanoparticles (AgNP)…………………………....5 1.1.4. Magnetic Iron Oxide Nanoparticles (IONP)……..……...6 1.2. Detection of Bacteria by Surface-Enhanced Raman Scattering (SERS)……………………………………………………………8 1.2.1 Background……………………………………………..8 1.2.2 Mechanism of SERS……………………………………9 1.2.3 Subtract of SERS………………………………………10 1.3. Capturing and Magnetic Separating of Bacteria……………...…13 1.3.1 Background...……………………………….…….…. . 13 1.3.2 Stabilization of Magnetic Nanoparticles……………… 14 1.3.3 Affinity Ligands to Bind the Target Cells………….…. 15 Chapter 2 Experimental Section………………………………................................. 19 2.1. Materials………………………………………………………...19 2.2. Exfoliation of Layered Mica into Random Nano Mica Platelets (NMP)……………………......................................................... .20 2.3. Synthesis of AgNP/NMP Nanohybrids………………………… 21 2.4. Synthesis of Iron oxide nanoparticles (Fe3O4)……………….….23 2.5. Synthesis of Fe3O4/NMP and Fe3O4/Mica Nanohybrids…..……24 2.6. Bacteria growth and sample Preparation………………………...25 2.7. Preparation of SERS Sample…....................................................26 2.8. Surface-Enhanced Raman Spectroscopy (SERS) Experiments....27 2.9 Bacterial Capture Efficiency of Fe3O4/Mica and Fe3O4/NMP…..28 2.10 Characterization…...………………………………………….... 29 Chapter 3 Results and Discussion…………………………………........................... 30 3.1. Synthesis of AgNP/Clay SERS Substrate……………….............30 3.1.1. Synthesis of AgNP/Clay Nanohybrids……………….. 30 3.1.2. 3D hot-junctions and flexibility in Ag/NMP SERS substrate………………………………………………. 35 3.1.3. Relationship between SERS intensity and inter-particle gap…………………………………………..................36 3.1.4. Differentiation of SERS signals between hydrophilic and hydrophobic bacteria………...........………………..….39 3.2. Synthesis of Magnetic Clay for Physically Capturing and Maneuvering Bacteria…………………………………………..41 3.2.1. Relative mobility rate of magnetically attracting clays with respect to clay platelet size and iron-oxide weight proportion in the Nanohybrids…………..……………..41 3.2.2. Synthesis of the Nanohybrids Comprising of Mica Nanoplatelets and Magnetic Iron-Oxide Nanoparticles..44 3.2.3. Synthesis and Characterization of Magnetic Iron Oxides on NMP………………………………………………..46 3.2.4. Capturing Microbial and Maneuverability under Magnetic Field ………………...……………..…………………..48 3.2.5. The Proposed Mechanism for Bacterial Capturing and Removing………...……………..……………………..52 Chapter 4 Conclusion…………………………………………………………….….55 Chapter 5 Future work…………………………………………………...……….….57 References…………………………………………………………...…...58 Figure Captions Figure 1-1. Morphology of nanomaterials (a) AgNP (b) CNTs (c) layer silicates…...2 Figure 1-2. Size comparison of naturally-occurring objects and some synthesized nanomaterials……………………………………………………..……..2 Figure 1-3. The structure of montmorillonite (MMT)……………………………….4 Figure 1-4. The scheme illustrates two strategies to fabricate multifunctional magnetic nanoparticles and their potential applications………………...7 Figure 1-5. Schemes for two fabrication strategies for SERS substrates using EBL. Two processes are presented. The left hand side process consists in an chemical etching that follows the electron beam exposing, the dissolution of the remaining PMMA layer, and deposition of metal. The substrate ends up with metal over the whole surface. The right hand side shows a metal deposition immediately after the e-beam exposition. After removal of the photoresistor layer, the substrate will present a series of isolated NPs, separated by regions where only bare Si substrate is exposed…………………………………………………………………11 Figure 1-6. (A) the process (a-f) for fabricating an array of AgNPs on porous anodic alumina surface of Ag/AAO SERS substrates. (B) top-view TEM image of Ag/AAO SERS substrate. (C) cross-sectional TEM image of Ag/AAO SERS substrate…………………………………………………………12 Figure 1-7. (A) Illustration of the capture of bacteria by vancomycin-conjugated magnetic nanoparticles via a plausible multivalent interaction (B) diameter)Van can bind to the terminal peptide, D-Ala-D-Ala, on the cell wall of a Gram-positive bacterium via hydrogen bonds……………….16 Figure 1-8. Cartoon representation of the vancomycin-D-alanyl-D-alanine interaction responsible for mediating the interaction between the nanoparticles and the bacteria. The critical components for the strong H-bonding interaction both on the vancomycin molecule (the heptapeptide backbone) and the D-alanyl-D-alanine dipeptide exposed from the bacterial surface are highlighted……………………………...16 Figure 1-9. TEM images of a Salmonella bacterium after binding to (a) bare MNPs, (b) antibody-immobilized MNPs, and (c) antibody-immobilized MNPs and antibody-immobilized TiO2. (d) A magnified view of the boxed region in (c)…………………………………………………………….17 Figure 1-10. Magnetic, porous, sugar-functionalized (MaPoS) PEG microgels are able to selectively bind and discriminate between different strains of bacteria Escherichia coli………………………………………………………...18 Figure 3-1. UV-Vis absorption of (a) AgNP/Mica and (b) AgNP/NMP nanohybrids in aqueous solution at 10 ppm silver concentration with different weight of AgNP/Clay..…….…………………………………………………...33 Figure 3-2. Transmission electron micrographs and size distribution (insert) of Ag at different weight ratios of Ag/Mica: (a) 1/99 (b) 7/93 and Ag/NMP: (a) 1/99 (b) 7/93 (c) 15/85, (d) 30/70, and (e) 50/50……………………....34 Figure 3-3. SERS spectra of S.aureus measured by various AgNP/NMP ratios of SERS substrates……………………………………………………......37 Figure 3-4. SERS spectra of E.coli measured by various AgNP/NMP ratios of SERS substrates……………………………………………………………….39 Figure 3-5. The bacterial cell wall structures of three different types bacterium. (a)Gram positive bacterium; (b) Gram-negative bacterium……………40 Figure 3-6. Relative maneuvering rate of four Fe3O4/clay under magnetic field…..42 Figure 3-7. TEM images and size distribution of (a) Pristine Fe3O4 (b) Fe3O4/Mica (c) Fe3O4/NMP(scale bar: 50μm)…………………..…………………45 Figure 3-8. Wide angle X-ray diffraction patterns of Fe3O4, Fe3O4/Mica and Fe3O4/NMP……………………………………………………..……47 Figure 3-9. Hysteresis curve of Fe3O4 (green line), Fe3O4/Mica (blue line) and Fe3O4/NMP (orange line) at 298 K………………………………….…47 Figure 3-10. Efficiency of the exfoliated NSP and NMP for capturing S. aureus from LB. The starting concentration (106, 105, 104, 103, and 102) of the SA in Fe3O4/ Clay solution (black column), S. aureus concentration in supernatant after magnetic separation of Fe3O4/Mica (blue column) and Fe3O4/NMP (purple column). The inset shows the photograph of SA (a) before magnetic capture (b) solution after magnetic capture by Fe3O4/Mica (c) solution after magnetic capture by Fe/NMP…………..50 Figure 3-11. Efficiency of the exfoliated NSP and NMP for capturing E. coli from LB. The starting concentration (106, 105, 104, and 103) of the EC in Fe3O4/ Clay solution (black column), E. coli concentration in supernatant after magnetic separation of Fe3O4/Mica (blue column) and Fe3O4/NMP (purple column). The inset shows the photograph of E. coli (a) before magnetic capture (b) solution after magnetic capture by Fe3O4/Mica (c) solution after magnetic capture by Fe3O4/NMP………………...……...51 Figure 3-12. SEM image of (a) pristine S. aureus after (b) Fe3O4/Mica treatment and (c) Fe3O4/NMP treatment (Scale bar: 1μm)……………………...…….54 Figure 3-13. SEM image of (a) pristine E.coli after (b) Fe3O4/Mica treatment and (c) Fe3O4/NMP treatment (Scale bar: 1μm)…………………...…………..54 Figure 5-1. AgNP/Fe3O4/NMP Captureing and Detecting Microbes…………...….57 Table Captions Table 1-1. Physical and magnetic properties of iron oxides………………………..7 Table 3-1. Relationship between SERS intensity and interparticle gap (W)/ diameter (D) ratio of AgNPs…………………………………………...38 Table 3-2. Magnetic separation of different Fe3O4/NMP and Fe3O4/Mica weight ratio…………………………………………………………………….43 Scheme Captions Scheme 3-1. Conceptual illustration of (a) clay exfoliation to NMP from Mice clay, (b) synthesis of AgNP on Mica (Ag/Mica), and (c) synthesis of AgNP on NMP (Ag/NMP)……………………………………..33 Scheme 3-2. Synthesis of the nanohybrids of Fe3O4/Nanoscale Mica Platelets (NMP)…………………………………………………………….45 Scheme 3-3. The mechanism of captured microbes by Fe3O4/NMP Nanohybrid……………………………………………………….53 | |
dc.language.iso | en | |
dc.title | 矽片/銀或磁鐵粒子之奈米複合物合成及其物理捕捉細菌應用 | zh_TW |
dc.title | Synthesizing Nanohybrids of Silicate Platelets/Silver or Magnetic Iron-Oxide Particles for Physically Capturing Bacteria | en |
dc.type | Thesis | |
dc.date.schoolyear | 102-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 謝國煌,劉定宇,胡淑文,張志豪 | |
dc.subject.keyword | 奈米矽片,奈米銀粒子,表面增強拉曼,奈米磁矽鐵粒子,捕捉細菌,分離細菌, | zh_TW |
dc.subject.keyword | nano-silicate-platelets,silver nanoparticles,surface-enhancing raman scattering,magnetic iron oxide nanoparticle,bacterial capturing,bacterial separating, | en |
dc.relation.page | 80 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2014-07-10 | |
dc.contributor.author-college | 工學院 | zh_TW |
dc.contributor.author-dept | 高分子科學與工程學研究所 | zh_TW |
顯示於系所單位: | 高分子科學與工程學研究所 |
文件中的檔案:
檔案 | 大小 | 格式 | |
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ntu-103-1.pdf 目前未授權公開取用 | 3.58 MB | Adobe PDF |
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