請用此 Handle URI 來引用此文件:
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/57473
完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 潘建源(Chien-Yuan Pan) | |
dc.contributor.author | Wan-Hsuan Hsu | en |
dc.contributor.author | 徐婉瑄 | zh_TW |
dc.date.accessioned | 2021-06-16T06:47:37Z | - |
dc.date.available | 2015-08-01 | |
dc.date.copyright | 2014-08-01 | |
dc.date.issued | 2014 | |
dc.date.submitted | 2014-07-25 | |
dc.identifier.citation | Amoroso, S., Schmid-Antomarchi, H., Fosset, M., and Lazdunski, M. (1990). Glucose, sulfonylureas, and neurotransmitter release: role of ATP-sensitive K+ channels. Science 247, 852-854.
Arjona, F.J., de Baaij, J.H., Schlingmann, K.P., Lameris, A.L., van Wijk, E., Flik, G., Regele, S., Korenke, G.C., Neophytou, B., Rust, S., et al. (2014). CNNM2 mutations cause impaired brain development and seizures in patients with hypomagnesemia. PLoS genetics 10, e1004267. Bear, M.F., Connors, B.W., and Paradiso, M.A. (2007). Neuroscience : exploring the brain, 3rd edn (Philadelphia, PA: Lippincott Williams & Wilkins). Bouron, A., and Reuter, H. (1996). A role of intracellular Na+ in the regulation of synaptic transmission and turnover of the vesicular pool in cultured hippocampal cells. Neuron 17, 969-978. Bush, A.I. (2000). Metals and neuroscience. Current opinion in chemical biology 4, 184-191. Cao, Y.Q., and Tsien, R.W. (2010). Different relationship of N- and P/Q-type Ca2+ channels to channel-interacting slots in controlling neurotransmission at cultured hippocampal synapses. The Journal of neuroscience : the official journal of the Society for Neuroscience 30, 4536-4546. Chang, K.S., Sun, C.J., Chiang, P.L., Chou, A.C., Lin, M.C., Liang, C., Hung, H.H., Yeh, Y.H., Chen, C.D., Pan, C.Y., and Chen, Y.T. (2012). Monitoring extracellular K+ flux with a valinomycin-coated silicon nanowire field-effect transistor. Biosensors & bioelectronics 31, 137-143. Chen, K.-I., Li, B.-R., and Chen, Y.-T. (2011). Silicon nanowire field-effect transistor-based biosensors for biomedical diagnosis and cellular recording investigation. Nano Today 6, 131-154. Chiang, P.L., Chou, T.C., Wu, T.H., Li, C.C., Liao, C.D., Lin, J.Y., Tsai, M.H., Tsai, C.C., Sun, C.J., Wang, C.H., et al. (2012). Nanowire transistor-based ultrasensitive virus detection with reversible surface functionalization. Chemistry, an Asian journal 7, 2073-2079. Clarke, N.D., and Berg, J.M. (1998). Zinc fingers in Caenorhabditis elegans: finding families and probing pathways. Science 282, 2018-2022. Cohen-Karni, T., Casanova, D., Cahoon, J.F., Qing, Q., Bell, D.C., and Lieber, C.M. (2012). Synthetically encoded ultrashort-channel nanowire transistors for fast, pointlike cellular signal detection. Nano letters 12, 2639-2644. Elfstrom, N., Karlstrom, A.E., and Linnros, J. (2008). Silicon nanoribbons for electrical detection of biomolecules. Nano letters 8, 945-949. Ganz, T. (2013). Systemic iron homeostasis. Physiological reviews 93, 1721-1741. Guieu, V., Ravelet, C., Perrier, S., Zhu, Z., Cayez, S., and Peyrin, E. (2011). Aptamer enzymatic cleavage protection assay for the gold nanoparticle-based colorimetric sensing of small molecules. Analytica chimica acta 706, 349-353. Hertz, L., and Dienel, G.A. (2005). Lactate transport and transporters: general principles and functional roles in brain cells. Journal of neuroscience research 79, 11-18. Hinke, J.A.M. (1961). THE MEASUREMENT OF SODIUM AND POTASSIUM ACTIVITIES IN THE SQUID AXON BY MEANS OF CATION-SELECTIVE GLASS MICRO-ELECTRODES. J Physiol (1961), 156, pp 314-335. Hung, H.H., Huang, W.P., and Pan, C.Y. (2013). Dopamine- and zinc-induced autophagosome formation facilitates PC12 cell survival. Cell biology and toxicology 29, 415-429. Kautz, L., and Nemeth, E. (2014). Molecular liaisons between erythropoiesis and iron metabolism. Blood. Kofuji, P., and Newman, E.A. (2009). Regulation of potassium by glial cells in the centralnervous system. 151-175. Kuo, H.C., Cheng, C.F., Clark, R.B., Lin, J.J., Lin, J.L., Hoshijima, M., Nguyen-Tran, V.T., Gu, Y., Ikeda, Y., Chu, P.H., et al. (2001). A defect in the Kv channel-interacting protein 2 (KChIP2) gene leads to a complete loss of I(to) and confers susceptibility to ventricular tachycardia. Cell 107, 801-813. Leblanc, N., and Hume, J.R. (1990). Sodium current-induced release of calcium from cardiac sarcoplasmic reticulum. Science 248, 372-376. Li, B.-R., Hsieh, Y.-J., Chen, Y.-X., Chung, Y.-T., Pan, C.-Y., and Chen, Y.-T. (2013a). An Ultrasensitive Nanowire-Transistor Biosensor for Detecting Dopamine Release from Living PC12 Cells under Hypoxic Stimulation. Journal of the American Chemical Society 135, 16034-16037. Li, B.R., Chen, C.C., Kumar, U.R., and Chen, Y.T. (2014). Advances in nanowire transistors for biological analysis and cellular investigation. The Analyst 139, 1589-1608. Li, Y., Ji, X., Song, W., and Guo, Y. (2013b). Design of a sensitive aptasensor based on magnetic microbeads-assisted strand displacement amplification and target recycling. Analytica chimica acta 770, 147-152. Lin, T.W., Hsieh, P.J., Lin, C.L., Fang, Y.Y., Yang, J.X., Tsai, C.C., Chiang, P.L., Pan, C.Y., and Chen, Y.T. (2010). Label-free detection of protein-protein interactions using a calmodulin-modified nanowire transistor. Proceedings of the National Academy of Sciences of the United States of America 107, 1047-1052. Lytton, J. (2007). Na+/Ca2+ exchangers: three mammalian gene families control Ca2+ transport. The Biochemical journal 406, 365-382. Maragakis, N.J., and Rothstein, J.D. (2004). Glutamate transporters: animal models to neurologic disease. Neurobiology of disease 15, 461-473. McCarthy, R.C., Park, Y.H., and Kosman, D.J. (2014). sAPP modulates iron efflux from brain microvascular endothelial cells by stabilizing the ferrous iron exporter ferroportin. EMBO reports. Meuwis, K., Boens, N., De Schryver, F.C., Gallay, J., and Vincent, M. (1995). Photophysics of the fluorescent K+ indicator PBFI. Biophysical journal 68, 2469-2473. Minta, A., and Tsien, R.Y. (1989). Fluorescent indicators for cytosolic sodium. The Journal of biological chemistry 264, 19449-19457. Musumeci, D., and Montesarchio, D. (2012). Polyvalent nucleic acid aptamers and modulation of their activity: a focus on the thrombin binding aptamer. Pharmacology & therapeutics 136, 202-215. Muyderman, H., Hansson, E., and Nilsson, M. (1997). Adrenoceptor-induced changes of intracellular K+ and Ca2+ in astrocytes and neurons in rat cortical primary cultures. Neuroscience letters 238, 33-36. Nagatoishi, S., Nojima, T., Galezowska, E., Gluszynska, A., Juskowiak, B., and Takenaka, S. (2007). Fluorescence energy transfer probes based on the guanine quadruplex formation for the fluorometric detection of potassium ion. Analytica chimica acta 581, 125-131. Ng, F., Mammen, O.K., Wilting, I., Sachs, G.S., Ferrier, I.N., Cassidy, F., Beaulieu, S., Yatham, L.N., Berk, M., and International Society for Bipolar, D. (2009). The International Society for Bipolar Disorders (ISBD) consensus guidelines for the safety monitoring of bipolar disorder treatments. Bipolar disorders 11, 559-595. Origa, R., Galanello, R., Ganz, T., Giagu, N., Maccioni, L., Faa, G., and Nemeth, E. (2007). Liver iron concentrations and urinary hepcidin in beta-thalassemia. Haematologica 92, 583-588. Ozaki, H., Nishihira, A., Wakabayashi, M., Kuwahara, M., and Sawai, H. (2006). Biomolecular sensor based on fluorescence-labeled aptamer. Bioorganic & medicinal chemistry letters 16, 4381-4384. Park, E.H., and Durand, D.M. (2006). Role of potassium lateral diffusion in non-synaptic epilepsy: a computational study. Journal of theoretical biology 238, 666-682. Pellerin, L., and Magistretti, P.J. (1994). Glutamate uptake into astrocytes stimulates aerobic glycolysis: a mechanism coupling neuronal activity to glucose utilization. Proceedings of the National Academy of Sciences of the United States of America 91, 10625-10629. Rinehart, J., Maksimova, Y.D., Tanis, J.E., Stone, K.L., Hodson, C.A., Zhang, J., Risinger, M., Pan, W., Wu, D., Colangelo, C.M., et al. (2009). Sites of regulated phosphorylation that control K-Cl cotransporter activity. Cell 138, 525-536. Rose, C.R. (1997). Intracellular Na+ Regulation in Neurons and Glia: Functional Implications. The Neuroscientist 3, 85-88. Sheldon, C., Diarra, A., Cheng, Y.M., and Church, J. (2004). Sodium influx pathways during and after anoxia in rat hippocampal neurons. The Journal of neuroscience : the official journal of the Society for Neuroscience 24, 11057-11069. Steel, A., and Hediger, M.A. (1998). The Molecular Physiology of Sodium- and Proton-Coupled Solute Transporters. News in physiological sciences : an international journal of physiology produced jointly by the International Union of Physiological Sciences and the American Physiological Society 13, 123-131. Takenaka, S., and Juskowiak, B. (2011). Fluorescence detection of potassium ion using the G-quadruplex structure. Analytical sciences : the international journal of the Japan Society for Analytical Chemistry 27, 1167-1172. Tominaga, M., and Takayama, Y. (2014). Interaction between TRP and Ca -activated chloride channels. Channels 8. Tsai, C.C., Chiang, P.L., Sun, C.J., Lin, T.W., Tsai, M.H., Chang, Y.C., and Chen, Y.T. (2011). Surface potential variations on a silicon nanowire transistor in biomolecular modification and detection. Nanotechnology 22, 135503. Tsien, A.M.a.R.Y. (1989). Fluorescent Indicators cytosolic sodium. Voma, C., Barfell, A., Croniger, C., and Romani, A. (2014). Reduced cellular Mg2+ content enhances hexose 6-phosphate dehydrogenase activity and expression in HepG2 and HL-60 cells. Archives of biochemistry and biophysics 548, 11-19. Wang, Q., Huang, J., Yang, X., Wang, K., He, L., Li, X., and Xue, C. (2011). Surface plasmon resonance detection of small molecule using split aptamer fragments. Sensors and Actuators B: Chemical 156, 893-898. Weilinger, N.L., Maslieieva, V., Bialecki, J., Sridharan, S.S., Tang, P.L., and Thompson, R.J. (2013). Ionotropic receptors and ion channels in ischemic neuronal death and dysfunction. Acta pharmacologica Sinica 34, 39-48. Xiao, A.Y., Homma, M., Wang, X.Q., Wang, X., and Yu, S.P. (2001). Role of K+ efflux in apoptosis induced by AMPA and kainate in mouse cortical neurons. Neuroscience 108, 61-67. Xie, X.M., and Smart, T.G. (1991). A physiological role for endogenous zinc in rat hippocampal synaptic neurotransmission. Nature 349, 521-524. Xu, J., Song, D., Xue, Z., Gu, L., Hertz, L., and Peng, L. (2013). Requirement of glycogenolysis for uptake of increased extracellular K+ in astrocytes: potential implications for K+ homeostasis and glycogen usage in brain. Neurochemical research 38, 472-485. Yu, S.P., Canzoniero, L.M., and Choi, D.W. (2001). Ion homeostasis and apoptosis. Current opinion in cell biology 13, 405-411. Yu, S.P., Yeh, C., Strasser, U., Tian, M., and Choi, D.W. (1999). NMDA receptor-mediated K+ efflux and neuronal apoptosis. Science 284, 336-339. Zhang, Y., Tian, J., Zhai, J., Luo, Y., Wang, L., Li, H., and Sun, X. (2011). Fluorescence-enhanced potassium ions detection based on inherent quenching ability of deoxyguanosines and K+-induced conformational transition of G-rich ssDNA from duplex to G-quadruplex structures. Journal of fluorescence 21, 1841-1846. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/57473 | - |
dc.description.abstract | 生物利用不同的離子來維持各種生理活性。如鈣離子參與神經傳導物質的釋放以及肌肉收縮。鈉離子是細胞外液中濃度最高的離子,對於調節血壓和維持血液循環的平衡扮演重要的角色,血清中的鈉離子增加會使血壓升高;而鈉離子和鉀離子的共同合作使神經衝動維持正常的傳遞。鉀離子是細胞內含量最多的離子,負責調控細胞膜的通透性以及細胞生長等多種細胞活性;最重要的是,鉀離子決定靜止膜電位以調控神經細胞的興奮性。由於細胞在組織間彼此靠得很緊密,當微環境中離子濃度改變時,細胞間彼此會互相影響。我們感興趣的是了解在細胞膜兩側的鈉、鉀離子濃度變化並確認鈉鉀離子對於細胞間微環境的影響。我們首先利用鈉離子專一性的螢光染劑,研究細胞內鈉離子的濃度變化。研究結果顯示,細胞內的鈉離子基本濃度是16.5 ± 1.6 mM,而在10 μM 麩胺酸刺激神經細胞後,神經細胞內鈉離子濃度上升到154.0 ± 68.2 mM,再慢慢回到基礎值; 而細胞內鉀離子基本濃度是142.5 ± 43.5 mM,而在10 μM 麩胺酸刺激神經細胞後,神經細胞內鉀離子濃度下降到10.6 ± 0.8 mM,但卻不再上升。而為了研究細胞膜表面的鉀離子濃度,我們將可與鉀離子結合的適體(aptamer)修飾在矽奈米線場效電晶體上。數據顯示此適體對於不同的鹼金族離子有不同的親和力:鉀 (Kd = 7.9 ± 0.4 mM) > 銫 (Kd = 12.1 ± 0.5 mM) > 鈉 (Kd = 24.8 ± 4.1 mM) >> 鋰 (Kd = 189.0 ± 44.5 mM)。最近我們正試著將細胞置於矽奈米線上,以偵測細胞膜表面的鉀離子濃度。綜合以上測量結果,可以讓我們對於神經細胞間相互作用有更詳細的了解。 | zh_TW |
dc.description.abstract | An organism utilizes different ions to support various physiological activities. Ca2+ participates in neurotransmitters release and muscle contraction. Na+ is the ion with highest concentration in the body fluid; the increase in the serum Na+ concentration elevates the blood pressure. In conjugation with K+, Na+ is involved in the transmission of nerve impulses. K+ is the most abundant ion inside the cell and regulates many cell activities like membrane permeability, growth, etc. Most importantly, K+ determines the resting membrane potential to modulate the neuron excitability. Because cells are in close contact with each other in tissues, the ion concentration changes at the interstitial microenvironment between cells will affect each other. Therefore, we are interested in understanding the concentrations of Na+ and K+ at both sides of the plasma membrane to verify their contributions to the microenvironment. We first used the Na+ specific fluorescence indicators to investigate the changes of intracellular Na+ concentration ([Na+]i) in primary-cultured neurons. The basal [Na+]i was 16.5 ± 1.6 mM and elevated to 154.0 ± 68.2 mM when stimulated by glutamate (10 μM); the concentration decline gradually afterwards. We used the K+ specific fluorescence indicators to investigate the changes of intracellular K+ concentration ([K+]i) in primary-cultured neurons. The basal [K+]i was 142.5 ± 43.5 mM and decreased to 10.6 ± 0.8 mM when stimulated by glutamate (10 μM); however, the [K+]i did not recover after the stimulation in 2 min. To investigate the K+ efflux at the membrane surface, we modified a K+-specific aptamer on the silicon nanowire field-effect transistor. The binding of K+ onto the apatamer changes the field effect surrounding the nanowire resulting in the conductivity changes. The data shows that the functionalized device responded to different alkali ions with various affinities: K+ (Kd = 7.9 ± 0.4 mM) > Cs+ (Kd = 12.1 ± 0.5 mM) > Na+ (Kd = 24.8 ± 4.1 mM) >> Li+ (Kd = 189.0 ± 44.5 mM). We are currently testing the K+ concentration at the membrane surface by anchoring cell onto the silicon nanowire. These measurements of the ion concentrations at the micro-environment will provide detail information in understanding the neuron-neuron interactions. | en |
dc.description.provenance | Made available in DSpace on 2021-06-16T06:47:37Z (GMT). No. of bitstreams: 1 ntu-103-R01b41024-1.pdf: 1232685 bytes, checksum: 28076a557d65f32e3428222c2007ae18 (MD5) Previous issue date: 2014 | en |
dc.description.tableofcontents | Catalog
致謝 I 中文摘要 II 英文摘要 III Introduction 1 The importance of ions in various physiological activities 1 Ionic concentrations at both sides of the plasma membrane 6 Roles of monovalent cations in neuron (Action potential) 7 The properties of ion channels 8 Gila cell regulate extracellular potassium concentration 9 Silicon nanowire field-effect transistors 11 G-quadruplex based aptamer 12 Aims 14 Materials and Methods 15 Chemicals 15 Primary culture of bovine chromaffin cells 15 Primary culture of cortical neurons 17 Fluorescence imaging 18 Calculated intracellular ions concentration 18 Silicon nanowire modification (Figure 1) 19 Electrical measurement 20 Data analysis 21 Results 23 The mechanism of Na+ influx and K+ efflux 23 DMPP elevates [Na+]i and decrease [K+]i in chromaffin cells 23 L-Glutamic acid elevates [Na+]i and decrease [K+]i in culture neurons 24 Calculated intracellular ions concentration 25 The bare SiNW-FET responses to pH changes 26 SiNW-FET detected K+ and Na+ 26 Neurons was stimulated by uncage glutamate in different [Na+] loading buffer 27 Neurons was stimulated with AMPA in different [Na+] loading buffer 28 SiNW-FET detected alkali ions and the calibrated response of Li+, Na+, K+ and CS+ 29 SiNW-FET response to K+ changes under 80 mM [Na+] 29 Discussion 30 The detection of [Na+] 30 The detection of [K+] 31 The [Na+] and [K+] play key role in membrane potential 32 The calculation of [K+]i 33 The FAT-5 aptamer modified SiNW-FET response to Li+, Na+, K+ and CS+ 34 The Debye length of 80 mM [Na+] buffer 34 Conclusion 35 Reference 36 Figures 41 | |
dc.language.iso | en | |
dc.title | 偵測細胞膜內外單價陽離子濃度 | zh_TW |
dc.title | Detecting the Monovalent Cation Concentrations at the Vicinity of the Plasma Membrane | en |
dc.type | Thesis | |
dc.date.schoolyear | 102-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 陳逸聰(Yit-Tsong Chen),謝如姬(Ru-Chi Shieh) | |
dc.subject.keyword | 鈉離子濃度,鉀離子濃度,細胞膜電位,神經傳導,矽奈米線場效電晶體, | zh_TW |
dc.subject.keyword | sodium ion concentration,potassium ion concentration,membrane potentia,neurotransmission,silicon nanowire field effect transistor, | en |
dc.relation.page | 55 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2014-07-25 | |
dc.contributor.author-college | 生命科學院 | zh_TW |
dc.contributor.author-dept | 生命科學系 | zh_TW |
顯示於系所單位: | 生命科學系 |
文件中的檔案:
檔案 | 大小 | 格式 | |
---|---|---|---|
ntu-103-1.pdf 目前未授權公開取用 | 1.2 MB | Adobe PDF |
系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。