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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 李世光(Chih-Kung Lee) | |
| dc.contributor.author | Guan-Wei Lee | en |
| dc.contributor.author | 李冠緯 | zh_TW |
| dc.date.accessioned | 2021-06-16T06:45:44Z | - |
| dc.date.available | 2014-08-01 | |
| dc.date.copyright | 2014-08-01 | |
| dc.date.issued | 2014 | |
| dc.date.submitted | 2014-07-28 | |
| dc.identifier.citation | [1] 台灣亞太產業分析專業協進會, 掌握未來趨勢∼發展台灣生醫科技產業, (2011).
[2] 吳宗正, 生物感測器, 九州出版社, 台北市 (1996). [3] A. E. Cass, G. Davis, G. D. Francis et al., “Ferrocene-mediated enzyme electrode for amperometric determination of glucose,” Analytical Chemistry, 56(4), 667-671 (1984). [4] X. Wu, Y. Chai, R. Yuan et al., “A novel label-free electrochemical microRNA biosensor using Pd nanoparticles as enhancer and linker,” Analyst, 138(4), 1060-1066 (2013). [5] O. Lazcka, F. J. D. Campo, and F. X. Muñoz, “Pathogen detection: A perspective of traditional methods and biosensors,” Biosensors and Bioelectronics, 22(7), 1205-1217 (2007). [6] N. Shao, E. Wickstrom, and B. Panchapakesan, “Nanotube–antibody biosensor arrays for the detection of circulating breast cancer cells,” Nanotechnology, 19(46), 465101 (2008). [7] J. Zhao, C. Chen, L. Zhang et al., “An electrochemical aptasensor based on hybridization chain reaction with enzyme-signal amplification for interferon-gamma detection,” Biosensors and Bioelectronics, 36(1), 129-134 (2012). [8] E. P. Randviir, and C. E. Banks, “Electrochemical impedance spectroscopy: an overview of bioanalytical applications,” Analytical Methods, 5(5), 1098-1115 (2013). [9] O. Pänke, T. Balkenhohl, J. Kafka et al., [Impedance spectroscopy and biosensing] Springer, (2008). [10] Y. Arima, M. Toda, and H. Iwata, “Surface plasmon resonance in monitoring of complement activation on biomaterials,” Advanced drug delivery reviews, 63(12), 988-999 (2011). [11] J. Lee, R. Chunara, W. Shen et al., “Suspended microchannel resonators with piezoresistive sensors,” Lab on a Chip, 11(4), 645-651 (2011). [12] “https://www.biacore.com/lifesciences/products/systems_overview/Biacore_4000/System-Information/index.html?backurl=%252Flifesciences%252Fproducts%252Fsystems_overview%252Findex.html..” [13] L. C. Clark, and C. Lyons, “Electrode systems for continuous monitoring in cardiovascular surgery,” Annals of the New York Academy of sciences, 102(1), 29-45 (1962). [14] 胡啟章, 電化學原理與方法, 五南圖書, 台北市 (2007). [15] D. Wang, G. Chen, H. Wang et al., “A reusable quartz crystal microbalance biosensor for highly specific detection of single-base DNA mutation,” Biosensors and Bioelectronics, 48, 276-280 (2013). [16] 周淑芬 and 陳建源, 免疫感測器之技術發展及其應用, CHEMISTRY (THE CHINESE CHEM. SOC., TAIPEI), 59(2), 263-271 (2001). [17] B. Xie, M. Mecklenburg, B. Danielsson et al., “Microbiosensor based on an integrated thermopile,” Analytica chimica acta, 299(2), 165-170 (1994). [18] A. Newman, K. Hunter, and W. Stanbro, 'The capacitive affinity sensor: a new biosensor.' 596-598. [19] J. S. Daniels, and N. Pourmand, “Label‐Free Impedance Biosensors: Opportunities and Challenges,” Electroanalysis, 19(12), 1239-1257 (2007). [20] R. F. Taylor, I. G. Marenchic, and E. J. Cook, “An acetylcholine receptor-based biosensor for the detection of cholinergic agents,” Analytica Chimica Acta, 213, 131-138 (1988). [21] R. F. Taylor, I. G. Marenchic, and R. H. Spencer, “Antibody-and receptor-based biosensors for detection and process control,” Analytica chimica acta, 249(1), 67-70 (1991). [22] V. M. Mirsky, M. Riepl, and O. S. Wolfbeis, “Capacitive monitoring of protein immobilization and antigen–antibody reactions on monomolecular alkylthiol films on gold electrodes,” Biosensors and Bioelectronics, 12(9), 977-989 (1997). [23] R. Maalouf, C. Fournier-Wirth, J. Coste et al., “Label-free detection of bacteria by electrochemical impedance spectroscopy: comparison to surface plasmon resonance,” Analytical chemistry, 79(13), 4879-4886 (2007). [24] V. Nandakumar, J. T. La Belle, J. Reed et al., “A methodology for rapid detection of< i> Salmonella typhimurium</i> using label-free electrochemical impedance spectroscopy,” Biosensors and Bioelectronics, 24(4), 1039-1042 (2008). [25] L. Yang, Y. Li, and G. F. Erf, “Interdigitated Array Microelectrode-Based Electrochemical Impedance Immunosensor for Detection of Escherichia c oli O157: H7,” Analytical chemistry, 76(4), 1107-1113 (2004). [26] M. Jie, C. Y. Ming, D. Jing et al., “An electrochemical impedance immunoanalytical method for detecting immunological interaction of human mammary tumor associated glycoprotein and its monoclonal antibody,” Electrochemistry communications, 1(9), 425-428 (1999). [27] S.-J. Ding, B.-W. Chang, C.-C. Wu et al., “Electrochemical evaluation of avidin–biotin interaction on self-assembled gold electrodes,” Electrochimica acta, 50(18), 3660-3666 (2005). [28] Y. Liu, N. Tuleouva, E. Ramanculov et al., “Aptamer-based electrochemical biosensor for interferon gamma detection,” Analytical Chemistry, 82(19), 8131-8136 (2010). [29] T. Hianik, and J. Wang, “Electrochemical aptasensors–recent achievements and perspectives,” Electroanalysis, 21(11), 1223-1235 (2009). [30] R. A. Potyrailo, R. C. Conrad, A. D. Ellington et al., “Adapting selected nucleic acid ligands (aptamers) to biosensors,” Analytical Chemistry, 70(16), 3419-3425 (1998). [31] D. Xu, D. Xu, X. Yu et al., “Label-free electrochemical detection for aptamer-based array electrodes,” Analytical Chemistry, 77(16), 5107-5113 (2005). [32] F. Darain, D.-S. Park, J.-S. Park et al., “Development of an immunosensor for the detection of vitellogenin using impedance spectroscopy,” Biosensors and Bioelectronics, 19(10), 1245-1252 (2004). [33] M. Wang, L. Wang, G. Wang et al., “Application of impedance spectroscopy for monitoring colloid Au-enhanced antibody immobilization and antibody–antigen reactions,” Biosensors and Bioelectronics, 19(6), 575-582 (2004). [34] 郭書辰, 丙型干擾素血液測驗於潛伏性結核病感染之角色, 感染控制雜誌, 23(1), 46-50 (2013). [35] 王品惠, 全血丙型干擾素釋放分析方法在結核病診斷與治療之應用, 成功大學公共衛生研究所學位論文, 1-67 (2011). [36] 蔣佳蓉, 潛伏性結核菌感染, 台灣醫事檢驗學會, (2011). [37] C. S. Chen, K. N. Chang, Y. H. Chen et al., “Development of a label-free impedance biosensor for detection of antibody-antigen interactions based on a novel conductive linker,” Biosensors and Bioelectronics, 26(6), 3072-3076 (2011). [38] “http://big5.39kf.com/cooperate/book/05/cell-biology/2007-09-25-411591.shtml.” [39] http://www.piercenet.com/product/nhs-sulfo-nhs. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/57429 | - |
| dc.description.abstract | 結核病是一全球性傳染病，在未開發及開發中國家尤其盛行，據世界衛生組織統計，全世界約有三分之一的人受到結核分枝桿菌感染，每秒約有一個人受到結核病的感染。丙型干擾素 (IFN-γ) 為肺結核的指標蛋白質，全血丙型干擾素檢驗 (IGRA) 被認為是取代結核菌素皮膚試驗 (TST) 成為潛伏結核感染 (LTBI) 最佳診斷方法，雖然透過酶免疫測定法來檢測IFN-γ已有了很大的進步，但此法仍有耗費人力和費時等缺點。為了改善上述缺點，本團隊採用IFN-γ抗體來檢測不同濃度的IFN-γ抗原，以檢測丙型干擾素為目標，期望能夠發展出一套具有免標定、高精準度、快速檢測的檢驗方法。本研究選擇以電化學方法作為生物感測器的開發基礎，並利用導電連結分子使蛋白質分子與金電極鍵結在一起以量測IFN-γ抗體抗原間相互反應。
在本論文中，我們利用電化學循環伏安法以及阻抗分析法來量測數種導電連結分子的導電特性。從實驗結果可以發現導電連結分子的導電度與碳鏈長短具有相當大的關係，當碳鏈越短時，導電的效果越好，致使訊雜比提升進而改善檢測極限。為了確保連結分子能夠鍵結在金電極上面，我們採用螢光顯微術來分析其鍵結效果。最後透過循環伏安法與電化學阻抗分析法來量測IFN-γ抗體抗原間的交互作用，實驗結果顯示此感測器在10 pM~50 nM的量測範圍內，阻抗隨著濃度上升而上升，但當濃度高於此範圍後，阻抗隨著濃度上升而逐漸下降，我們推測造成此現象的原因，主要為此電化學系統中空間障礙與靜電作用力相互競爭所造成的結果。 | zh_TW |
| dc.description.abstract | Tuberculosis (TB) is an ancient disease constituted a long-term menace to public health. According to World Health Organization (WHO), mycobacterium tuberculosis (MTB) infected nearly a third of people of the world. There is about one new TB occurrence every second worldwide today. Interferon-gamma (IFN-γ) is associated with susceptibility to TB, and interferon-gamma release assays (IGRA) is considered to be the best alternative of tuberculin skin test (TST) for diagnosis of latent tuberculosis infection (LTBI). Although significant progress has been made with regard to the design of enzyme immunoassays for IFN-γ, adopting this assay is still labor-intensive and time-consuming. To alleviate these drawbacks, we used IFN-γ antibody to facilitate the detection of IFN-γ. Our team chose IFN-γ as the target protein and expected to pursue a label-free, high-precision, and rapid detection technique. We chose electrochemical method as the development foundation of the biosensor and adopted conductive linker to form a self-assembled monolayer (SAM). Through the impedance measurement, we can understand the interaction of IFN-γ antibody and IFN-γantigen.
In the thesis, cyclic voltammetry and electrochemical impedance spectroscopy are used to measure the conductive characteristics of three kinds of conductive linkers. From the experimental results, it can be found that conductivity is associated with the number of methylene chain. As the number of methylene chain decreased, the conductivity increased. Through the conductivity enhancement, we can obtain a higher signal/noise ratio such that the detection limit is improved. Fluorescence microscopy was used to verify that the conductive linkers have capability to bind with gold. Finally, we used the electrochemical methods to observe the interaction of IFN-γ antibody and IFN-γ antigen. The results show that for the range from 10 pM to 50 nM, the impedance would increase along with the rising of IFN-γ concentration. When the concentration exceeded this range, the impedance would decrease along with the rising of IFN-γ concentration. It is our conjecture that these two trends can be attributed to the competition between steric hindrance and electrostatic force. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-16T06:45:44Z (GMT). No. of bitstreams: 1 ntu-103-R01543001-1.pdf: 4113790 bytes, checksum: 5c3108f3cf06bf2f30ee56f0b8e6a95c (MD5) Previous issue date: 2014 | en |
| dc.description.tableofcontents | CONTENTS
口試委員會審定書 # 中文摘要 iii ABSTRACT iv CONTENTS vi 圖目錄 ix 表目錄 xii Chapter 1 緒論 1 1.1 研究背景 1 1.2 文獻回顧 8 1.3 研究動機 13 Chapter 2 實驗原理 16 2.1 電化學基本原理 16 2.1.1 前言 16 2.1.2 法拉第與非法拉第程序 18 2.1.3 電化學反應程序 21 2.1.4 電化學槽與其電阻 23 2.1.5 半反應與電極電位 23 2.1.6 參考電極 26 2.1.7 線性掃描伏安法 (Linear Sweep Voltammetry, LSV) 27 2.1.8 循環伏安法 28 2.2 電化學阻抗頻譜分析原理 29 2.2.1 交流電之電路原理 30 2.2.2 Randles 等效電路 31 2.2.3 電化學阻抗交流頻譜分析 35 2.3 螢光顯微術基本原理 37 Chapter 3 實驗架設 38 3.1 電化學量測方法 38 3.1.1 實驗設備 38 3.1.2 連接分子與生物分子 44 3.1.3 化學試劑與其他溶液 44 3.1.4 電極表面修飾方法 45 3.1.5 電化學量測流程 47 3.2 螢光顯微術量測方法 51 3.2.1 晶片修飾步驟 51 3.2.2 量測步驟 51 Chapter 4 實驗結果分析與討論 54 4.1 以螢光顯微術量測連結分子之鍵結效果 54 4.2 電化學量測結果 56 4.2.1 連結分子阻抗量測結果 56 4.2.2 抗體與導電連接分子的固定化 58 4.2.3 以CS20S為連結分子之IFN-γ抗體抗原反應 61 Chapter 5 結論與未來展望 72 5.1 結論 72 5.2 未來展望 73 參考文獻 74 | |
| dc.language.iso | zh-TW | |
| dc.subject | 電化學阻抗頻譜分析 | zh_TW |
| dc.subject | 導電連結分子 | zh_TW |
| dc.subject | 丙型干擾素 | zh_TW |
| dc.subject | 生物感測器 | zh_TW |
| dc.subject | 肺結核 | zh_TW |
| dc.subject | IFN-γ | en |
| dc.subject | EIS | en |
| dc.subject | conductive linker | en |
| dc.subject | biosensor | en |
| dc.subject | Tuberculosis | en |
| dc.title | 整合導電連接分子與電化學阻抗分析儀以檢測丙型干擾素之研究 | zh_TW |
| dc.title | Detection of Interferon-gamma based-on Integration of Conductive Linker and Electrochemical Impedance Spectroscopy | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 102-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 李世元(Shih-Yuan Lee),何國川(Kuo-Chuan Ho),李舒昇(Shu-Sheng Lee),黃念祖(Nien-Tsu Huang) | |
| dc.subject.keyword | 電化學阻抗頻譜分析,導電連結分子,丙型干擾素,生物感測器,肺結核, | zh_TW |
| dc.subject.keyword | IFN-γ,EIS,conductive linker,biosensor,Tuberculosis, | en |
| dc.relation.page | 76 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2014-07-28 | |
| dc.contributor.author-college | 工學院 | zh_TW |
| dc.contributor.author-dept | 應用力學研究所 | zh_TW |
| 顯示於系所單位： | 應用力學研究所 | |
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