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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 林俊彬(Chun-Pin Lin) | |
dc.contributor.author | Wei-Sheng Yang | en |
dc.contributor.author | 楊為盛 | zh_TW |
dc.date.accessioned | 2021-06-16T06:40:06Z | - |
dc.date.available | 2014-10-15 | |
dc.date.copyright | 2014-10-15 | |
dc.date.issued | 2014 | |
dc.date.submitted | 2014-07-30 | |
dc.identifier.citation | 1. Torabinejad, M. and T. Ford, Root end filling materials: a review. Dental Traumatology, 1996. 12(4): p. 161-178.
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dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/57277 | - |
dc.description.abstract | 牙科的治療日新月異,其目的都是想要保持牙齒的健康。在根管治療中,根尖手術和逆充填、根管破損修補、活髓治療,這幾項難題的是我們所要面對的問題中,十分棘手的病症。隨著生醫材料的發展,這些問題有逐漸獲得改善。
Mineral trioxide aggregate (MTA)因具有生物相容性、生物活性、封閉性質佳等優點,受到牙科醫師的推崇和喜愛。但是 MTA 硬化時間過長、操作性質不佳與可能含有有毒重金屬等問題卻是臨床應用的重大缺點。許多研究顯示 MTA 良好的生物相容性、生物活性以及封閉能力,主要是因為它是鈣矽生醫陶瓷的關係,同樣的問題也是發生在鈣矽生醫陶瓷上,就是有硬化時間較長的問題。 本團隊在之前的研究之中有發現,以鈣矽生醫陶瓷作為基礎的材料,加入過渡元素之後,可以改善它的機械性制和生物特性,我們將這樣的材料稱為介穩水泥。鋅元素是過渡元素的一種,而他在醫學上的功用,受到大家廣泛的討論。鋅不僅能夠促進細胞生長,也能夠調節骨細胞的分化和形成、硬組織的分化,。鋅也在抗菌方面,也被許多醫療器材當作表面抗菌材料。本團隊已MTA為基礎,也就是鈣矽生醫陶瓷為改良,將鋅元素在材料燒結的過程,摻入到鈣矽生醫陶瓷之中,生產出含鋅的介穩水泥,企圖改善其機械性質,和生物相容性及生物活性,並和市售材料MTA作相互比較。 結果發現在機械性質方面,我們可以看到,介穩水泥加入鋅之後可以讓硬化時間大量的縮短,而在抗壓強度和微硬度方面,雖然略低於MTA,但都差距不大,我們推論這可能是和它的水合作用的快慢有關。在水溶液中的酸鹼值和離子釋放濃度方面,可以看到,介穩水泥加鋅之後的酸鹼值,都比MTA還來的接近原來pH值,這樣的情形可以推論,我們的水溶液酸鹼值更適合細胞存活,而這個推論,可以在生物相容性的實驗中獲得到證實。在生物相容性的實驗,介穩水泥加鋅之後的死亡率,比MTA和氫氧化鈣而言都有明顯下降的現象。另外在抗菌的部分的表現,我們發現它在前六個小時的抑菌效果比較明顯,但是時間拉長之後,其效果就開始減低,我們推論會造成這樣的結果,可能是因為E. faecalis對鹼性有比較好的耐受性關係。此外,鋅離子的釋放量,也低於鋅離子的最小殺菌濃度。最後,我們在生物活性方面,觀察結果發現,加鋅之後的介穩水泥,和MTA一樣都有促進鹼性磷酸酶增加的現象,我們可以推論說,含鋅的介穩水泥有刺激細胞分化和礦化的能力。最後,有加鋅的介穩水泥,在鈣離子的釋放量減少,代表著材料的溶解於水中的比例減少,我們可以在近一步大膽假設它的封閉性更好,而這也是我們在未來的實驗中可以再去證實的。 總結來說,介穩水泥加入鋅進去之後,以市售材料MTA相互比較,其硬化時間明顯減少,而且抗壓強度和微硬度接近MTA;其酸鹼值和離子釋放變化也減少,更接近人體的體液,所以不會造成細胞傷害,而且也可以含鋅介穩水泥可以促進細胞的分化和礦化。 | zh_TW |
dc.description.abstract | To keep the tooth health, the technology of dental treatment is continuously processing improved. Among the sticky problems, including apical surgery and retrograde filling, root canal perforation repair, and vital pulp therapy, we are difficult to handle.
Fortunately, we could solve this problem by applying the biomaterial improvement. MTA which based on calcium silicate bioceramics is one of the alternatives that be widely applied. As the MTA serves several advantages such as good biocompatibility, bioactive and good sealing ability, it’s most widely applied at the current stage. However, MTA is not an ideal material, for example, it takes long setting time, poor handling property and toxic ions contained, it turns to be critical in clinical application. According to many studies of MTA, many advantages of MTA are based on its main component: calcium silicate, however, our previous study showed that calcium silicate bioceramics had slow hydration rate and long setting time. Our group discovered such transitional elements that added to the calcium silicate cement, it would improve its material properties and we called this new material as Partial-Stabilized Cement (PSC). Zn is an essential trace element, that plays an important role in the body growth and development. Nevertheless, Zn also plays an important role in bone regulation, bone mineralization, stimulation of bone, inhibition of bone resorption, cell replication and differentiation of osteoblast cell. Furthermore, zinc plays an important role in medical material, because it would inhibit microbial growth. The aim of this study is to evaluate the effect of zinc content on material properties, biocompatibility and bioactivity of PSC. About the result, we could discover that the zinc doped to the PSC would reduce the setting time. The compressive strength and microhardness were lower than MTA, but it serves no significant differences. In the pH value, the pH value of PSC+Zn groups were closed to original value, it means this environment was much suitable for cell survival. The conclusion was confirmed form the data that the cell mortal rates of PSC+Zn groups were lower than MTA and Ca(OH)2 group. In the antibacterial test, the all experiment groups were better than control groups during 6 hours, but there were no significant different between all groups after 12 hours. This may be because of some alkalo-tolerant characteristics enabling the reduced susceptibility of E. faecalis. In addition, the zinc ion releasing concentration was lower than the minimum inhibitory concentration. In the bioactivity test, the all PSC+Zn groups were as well as MTA groups. Based upon such test, we could conclude that PSC+Zn groups were stimulate cell differentiation and mineralization. Finally, we can make some conclusions from this study. Firstly, the zinc added to the PSC would improve its material properties. Secondly, The PSC+Zn groups were good at biocompability and bioactivity. | en |
dc.description.provenance | Made available in DSpace on 2021-06-16T06:40:06Z (GMT). No. of bitstreams: 1 ntu-103-R00422017-1.pdf: 21045726 bytes, checksum: 7549fd258a4deafd1dc214d3c4129317 (MD5) Previous issue date: 2014 | en |
dc.description.tableofcontents | 謝誌 I
中文摘要 II ABSTRACT IV 目次 VI 圖表目錄 XI 第1章 前言 1 第2章 文獻回顧 3 2.1 牙齒的構造與結構 3 2.2 根管治療的目的 4 2.3 根尖手術與逆充填 5 2.4 根管穿孔修補 6 2.5 活髓治療 6 2.6 牙髓病修補材料 7 2.7 生醫材料 8 2.8 生醫陶瓷 9 2.9 三氧礦化物 10 2.10 鈣矽生醫陶瓷 11 2.11 介穩水泥 12 2.12 過度元素的添加對介穩水泥的影響 14 2.13 鋅的影響 15 2.14 溶膠-凝膠法 16 第3章 動機和目的 18 第4章 實驗材料製備與評估 19 4.1.1 材料製備 19 4.1.2 X光繞射評估分析材料粉末 19 4.2 硬化時間測試 20 4.2.1 測試樣本製備 20 4.2.2 測試方法 20 4.2.3 資料分析 21 4.3 抗壓強度測試 21 4.3.1 測試樣本製備 21 4.3.2 抗壓強度測試 21 4.3.3 實驗過程和紀錄 22 4.3.4 結果分析 22 4.4 表面微硬度測試 23 4.4.1 測試樣本製備 23 4.4.2 微硬度儀器規格 23 4.4.3 測試方法 23 4.4.4 資料分析 24 4.5 酸鹼值測試 24 4.5.1 模擬體液溶液SBF之製作 24 4.5.2 測試樣本製備 24 4.5.3 實驗過程和紀錄 25 4.6 離子釋放濃度測試 25 4.6.1 感應耦合電漿質譜分析儀(ICP-MS)校正 25 4.6.2 測試樣本製備 25 4.6.3 實驗過程和紀錄 26 4.7 材料抑菌效果評估 26 4.7.1 菌種培養 26 4.7.2 測試樣本製備 26 4.7.3 實驗過程和紀錄 27 4.8 材料生物相容性評估 27 4.8.1 人類牙髓細胞和牙周韌帶細胞初級培養 27 4.8.2 細胞貼附試驗 28 4.8.3 萃取液製備 28 4.8.4 WST-1 29 4.8.5 LDH 29 4.9 鹼性磷酸酶定性染色分析 31 4.9.1 Stock substrate solution配置 31 4.9.2 實驗步驟 31 4.10 鹼性磷酸酶定量染色分析 32 4.10.1 實驗步驟 32 4.11 動物實驗 33 4.11.1 實驗步驟 33 4.11.2 動物灌流 34 4.11.3 標本製作 35 4.11.4 Micro - CT照射 36 第5章 結果 37 5.1 硬化時間測試 37 5.2 抗壓強度測試 37 5.3 表面微硬度測試 38 5.4 水合產物之酸鹼值變化 39 5.5 水合產物之離子濃度變化 40 5.6 材料抑菌效果評估 40 5.7 材料生物相容性評估 41 5.7.1 細胞貼附試驗 41 5.7.2 WST-1 41 5.7.3 LDH 41 5.8 鹼性磷酸酶定性染色分析 42 5.9 鹼性磷酸酶定量分析 42 5.10 動物實驗 43 第6章 討論 44 6.1 添加鋅之後對材料機械性質的影響 44 6.2 添加鋅之後對酸鹼值變化 47 6.3 添加鋅之後對離子釋放量的變化 48 6.4 添加鋅之後對材料抑菌效果的影響 49 6.5 添加鋅之後對材料生物相容性的影響 51 6.6 添加鋅之後對其生物礦化能力的影響 53 第7章 結論 58 第8章 未來研究方向 59 第9章 圖表目錄 60 第10章 參考資料 99 | |
dc.language.iso | zh-TW | |
dc.title | 鋅含量對介穩水泥的材料性質和生物相容性及生物活性之影響 | zh_TW |
dc.title | Effect of Zinc Content on Material Properties, Biocompatibility and Bioactivity of Partial-stabilized Cements | en |
dc.type | Thesis | |
dc.date.schoolyear | 102-2 | |
dc.description.degree | 碩士 | |
dc.contributor.coadvisor | 林?輝(Feng-Huei Lin) | |
dc.contributor.oralexamcommittee | 鄧麗珍,章浩宏,姜昱至 | |
dc.subject.keyword | 介穩水泥,生醫陶瓷,含鋅鈣陶瓷,抗菌,生物活性,生物相容性, | zh_TW |
dc.subject.keyword | Partial-stabilized cement,bioceramics,zinc contained calcium silicate ceramic,antimicrobial ability,bioactivity,biocompability, | en |
dc.relation.page | 106 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2014-07-30 | |
dc.contributor.author-college | 牙醫專業學院 | zh_TW |
dc.contributor.author-dept | 臨床牙醫學研究所 | zh_TW |
顯示於系所單位: | 臨床牙醫學研究所 |
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