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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 林璧鳳 | |
dc.contributor.author | Ya-Ching Chang | en |
dc.contributor.author | 詹雅晴 | zh_TW |
dc.date.accessioned | 2021-06-16T06:37:53Z | - |
dc.date.available | 2019-08-04 | |
dc.date.copyright | 2014-08-04 | |
dc.date.issued | 2014 | |
dc.date.submitted | 2014-07-30 | |
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dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/57208 | - |
dc.description.abstract | 益生菌是指對宿主有正面效益的活性微生物,藉由活化腸道內的巨噬細胞或樹突細胞進而刺激或調節宿主的免疫系統;魚油藉由降低吞噬細胞活性、減少發炎激素分泌方式抑制過度或慢性的發炎反應。本實驗探討合併補充兩種具免疫調節能力的益生菌與含魚油的綜合營養劑對免疫調節的效果是否比單獨補充顯著。
本實驗檢測的益生菌為Lactobacilus rhamnosus及Bifidobacterium lactis之混合物;綜合營養劑除了魚油外含有多種微量營養素。實驗分成兩部分,分別為非特異性及特異性免疫反應。非特異性免疫反應實驗中,利用九週大的BALB/c小鼠,管餵樣品六週後觀察其先天性及非特異之適應性免疫指標,結果合併補充的組別較單一樣品補充顯著提升自然殺手細胞活性、提升血清總IgM、IgG及IgA含量及降低脾臟細胞IL-2分泌量。得知合併補充較具免疫調節能力後,接以進行特異性免疫反應實驗,同樣以九週大的BALB/c小鼠進行OVA致免模式,檢測指標除了有非特異性免疫指標,亦含OVA特異性抗體及脾臟細胞在OVA刺激下激素的檢測。結果顯示合併補充組別顯著促進B細胞增生、血清總IgG及IgA含量及脾臟細胞IL-10分泌量;血液吞噬細胞活性、T細胞增生有增加的趨勢,卻依然降低脾臟細胞IL-2分泌量。合併補充組別特異性免疫結果在血清OVA-specific IgG有較高趨勢,在OVA刺激下脾臟細胞IL-2分泌量顯著上升。 綜合以上結果得知合併補充益生菌及含魚油之綜合營養劑相較單一補充的組別,較能提升BALB/c小鼠免疫細胞的活化及免疫系統的調節能力,其中,最明顯的是提升血清總抗體含量,顯示小鼠抵抗致病原感染的能力及黏膜免疫都有提升的現象。 | zh_TW |
dc.description.abstract | Probiotics is defined as the microbe that beneficially affect the host. By influencing the intestinal macrophage and dendritic cell, probiotics may stimulate the host immune response or promote the immune-modulation function. Fish oil alleviates the excessive and chronic inflammation by reducing the phagocytic activity of macrophage and pro-inflammatory cytokine level. Therefore, we wondered that whether the combination of probiotics and nutrition supplement containing fish oil would multiple the effects of immune-modulatory function.
In this study, the mixture of Lactobacilus rhamnosus and Bifidobacterium lactis were used as the source of probiotics and the nutrition supplement contains fish oil and various micronutrients. There were two parts in this experiment: non-specific and specific immune response. In the first part, nine-week-old BALB/c female mice were tube-fed with different contents including probiotics alone, nutrition supplement alone or combined sample for 6 weeks and followed by performing sacrifice to measure the innate and non-specific adaptive immune indicators. The results showed that combined-sample group significantly increased activity of NK cell, elevated total IgM, IgG, IgA in the serum and reduced IL-2 secretion of splenocytes. Knowing that the immune-modulatory ability of combined-sample group, we continued with the next part. In the experiment, nine-week-old BALB/c female mice were not only fed by different sample for 9 weeks but also immunized with OVA, followed by sacrifice. The innate immune responses and adaptive immune responses including OVA-specific and non-specific were examined. The results showed that combined-sample group significantly induced B cell proliferation, elevated total IgG, IgA in the serum, and significantly increased IL-10 but reduced IL-2 secretion of splenocytes. Combined-sample group also tend to induce phagocytic activity and T cell proliferation. In the OVA-specific indicators, combined-sample group tend to increase OVA-specific IgG and significantly increased IL-2 secretion with OVA- stimulated splenocytes. In conclusion, combined-sample group is the most remarkable to modulate the immune system within other single-sample groups, especially the elevated level of total antibodies. Supporting that supplied BALB/c mice with probiotics combined nutrition supplement containing fish oil may benefit the immune system than the single sample group, which may avoid the extracellular pathogen invasion and promote mucosal immunity. | en |
dc.description.provenance | Made available in DSpace on 2021-06-16T06:37:53Z (GMT). No. of bitstreams: 1 ntu-103-R01b22043-1.pdf: 1995065 bytes, checksum: fd318f6bec4a0a6e5be7124db1d70879 (MD5) Previous issue date: 2014 | en |
dc.description.tableofcontents | 前言 1
第一章 文獻回顧 3 第一節 免疫系統概述 3 一、 先天性免疫反應 3 二、 適應性免疫反應 5 1. T細胞 5 2. B細胞 7 第二節 營養素與免疫系統 8 第三節 益生菌與免疫系統 12 第四節 營養素與益生菌的協同作用 16 第五節 研究動機 17 第二章 非特異性免疫反應 18 第一節 前言 18 第二節 材料方法 18 一、 實驗動物 18 二、 組別及劑量計算 19 三、 實驗流程及分析項目 22 1. 實驗流程 22 2. 分析項目: 22 四、 樣品收集與分析 23 1. 血液樣品收集 23 2. 血液吞噬細胞活性分析 23 3. 小鼠犧牲 24 4. 腹腔細胞的取得 25 5. 脾臟細胞的取得 26 6. 脾臟自然殺手細胞活性分析 27 7. 脾臟細胞表型的分析 28 8. 脾臟細胞增生能力的測定 29 9. 腹腔/脾臟細胞激素分泌量的測定 30 10. 血清中非特異性抗體的測定 32 11. 統計方法 33 第三節 結果 34 一、 體重變化及攝食量 34 二、 組織絕對/相對重量 36 三、 血液吞噬細胞的活性 37 四、 脾臟細胞自然殺手細胞的活性 38 五、 脾臟淋巴細胞亞群分佈比例 39 六、 脾臟細胞增生能力 40 七、 血清非特異性抗體的含量 40 八、 脾臟細胞細胞激素 42 九、 腹腔細胞細胞激素 45 第四節 討論 46 一、 小鼠組織重量 46 二、 先天性免疫反應 46 三、 適應性免疫反應 47 1. 脾臟淋巴細胞亞群分佈比例及細胞增生能力 47 2. 抗體 47 3. 細胞激素 48 (1). 脾臟細胞激素 48 (2). 腹腔細胞激素 48 第三章 特異性免疫反應 49 第一節 前言 49 第二節 材料方法 50 一、 實驗動物 50 二、 組別及劑量計算 50 三、 實驗流程及分析項目 51 1. 實驗流程 51 2. 分析項目 51 四、 特異性免疫實驗操作模式 51 五、 樣品收集與分析 52 1. 血液樣品收集 52 2. 血液吞噬細胞活性分析 52 3. 小鼠犧牲 52 4. 腹腔細胞的取得與培養 52 5. 脾臟細胞的取得與培養 52 6. 脾臟自然殺手細胞活性分析 52 7. 脾臟細胞表型的分析 52 8. 脾臟細胞增生能力的測定 53 9. 腹腔/脾臟細胞激素分泌量的測定 53 10. 血清非特異性抗體的測定 53 11. 血清中特異性抗體 53 12. 統計方法 54 第三節 結果 55 一、 體重變化及攝食量 55 二、 組織絕對/相對重量 56 三、 血液吞噬細胞的活性 58 四、 脾臟細胞自然殺手細胞活性 59 五、 脾臟淋巴細胞亞群分佈比例 60 六、 脾臟細胞增生能力 61 七、 血清非特異性抗體的含量 62 八、 血清特異性抗體的含量 63 九、 脾臟細胞細胞激素 65 十、 腹腔細胞細胞激素 68 第四節 討論 69 一、 小鼠組織重量 69 二、 先天性免疫反應 69 三、 適應性免疫反應 69 1. 脾臟淋巴細胞亞群分佈比例及細胞增生能力 69 2. 抗體 70 (1). 非特異性抗體 70 (2). OVA特異性抗體 70 3. 細胞激素 70 第四章 綜合討論與總結 72 第一節 綜合討論 72 一、 單獨補充益生菌 72 二、 單獨補充綜合營養劑 73 三、 合併補充綜和營養劑及益生菌 74 四、 腸道免疫與腸道菌相討論 78 第二節 總結 79 第五章 參考文獻 80 | |
dc.language.iso | zh-TW | |
dc.title | 合併補充益生菌及含魚油之綜合營養劑對BALB/c小鼠免疫的影響 | zh_TW |
dc.title | The combined effects of probiotics and nutrition supplement containing fish oil on immune response in BALB/c Mice | en |
dc.type | Thesis | |
dc.date.schoolyear | 102-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 江孟燦,江伯倫,林金源,洪永瀚 | |
dc.subject.keyword | 免疫調節,Lactobacilus rhamnosus及Bifidobacterium lactis,魚油,微量營養素, | zh_TW |
dc.subject.keyword | immune-modulatory,Lactobacilus rhamnosus and Bifidobacterium lactis,fish oil,micronutrients, | en |
dc.relation.page | 108 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2014-07-31 | |
dc.contributor.author-college | 生命科學院 | zh_TW |
dc.contributor.author-dept | 生化科技學系 | zh_TW |
顯示於系所單位: | 生化科技學系 |
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