請用此 Handle URI 來引用此文件：
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/56967完整後設資料紀錄
| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 馮哲川(Zhe Chuan Feng) | |
| dc.contributor.author | Shu-Wen Chen | en |
| dc.contributor.author | 陳述文 | zh_TW |
| dc.date.accessioned | 2021-06-16T06:32:04Z | - |
| dc.date.available | 2016-08-16 | |
| dc.date.copyright | 2014-08-16 | |
| dc.date.issued | 2014 | |
| dc.date.submitted | 2014-08-06 | |
| dc.identifier.citation | [1] J. Y. Chen, C. J. Chen, M. Y. Liu, S. M. Cho, M. M. Hsu, T. C. Lynn, et al., 'Antibody to Epstein‐Barr virus‐specific DNase as a marker for field survey of patients with nasopharyngeal carcinoma in Taiwan,' Journal of medical virology, vol. 27, pp. 269-273, 1989.
[2] J. Fachiroh, T. Schouten, B. Hariwiyanto, D. K. Paramita, A. Harijadi, S. M. Haryana, et al., 'Molecular diversity of Epstein-Barr virus IgG and IgA antibody responses in nasopharyngeal carcinoma: a comparison of Indonesian, Chinese, and European subjects,' Journal of Infectious Diseases, vol. 190, pp. 53-62, 2004. [3] A. S. Fauci, Harrison's principles of internal medicine vol. 2: McGraw-Hill Medical New York, 2008. [4] K.-C. Chow, J. Ma, L.-S. Lin, K.-H. Chi, S.-H. Yen, S.-M. Liu, et al., 'Serum responses to the combination of Epstein-Barr virus antigens from both latent and acute phases in nasopharyngeal carcinoma: complementary test of EBNA-1 with EA-D,' Cancer Epidemiology Biomarkers & Prevention, vol. 6, pp. 363-368, 1997. [5] J. Luka, R. C. Chase, and G. R. Pearson, 'A sensitive enzyme-linked immunosorbent assay (ELISA) against the major EBV-associated antigens. I. Correlation between ELISA and immunofluorescence titers using purified antigens,' Journal of immunological methods, vol. 67, pp. 145-156, 1984. [6] J. Gray, 'Avidity of EBV VCA-specific IgG antibodies: distinction between recent primary infection, past infection and reactivation,' Journal of virological methods, vol. 52, pp. 95-104, 1995. [7] M. Kunimoto, S. Tamura, T. Tabata, and O. Yoshie, 'One-step typing of Epstein-Barr virus by polymerase chain reaction: predominance of type 1 virus in Japan,' The Journal of general virology, vol. 73, pp. 455-461, 1992. [8] C. V. Sapan, R. L. Lundblad, and N. C. Price, 'Colorimetric protein assay techniques,' Biotechnology and applied Biochemistry, vol. 29, pp. 99-108, 1999. [9] T. Riedel, C. Rodriguez-Emmenegger, A. de los Santos Pereira, A. Bědajankova, P. Jinoch, P. M. Boltovets, et al., 'Diagnosis of Epstein–Barr virus infection in clinical serum samples by an SPR biosensor assay,' Biosensors and Bioelectronics, vol. 55, pp. 278-284, 2014. [10] M. J. Schoning and A. Poghossian, 'Bio FEDs (Field‐Effect Devices): State‐of‐the‐Art and New Directions,' Electroanalysis, vol. 18, pp. 1893-1900, 2006. [11] T. Sakata, S. Matsumoto, Y. Nakajima, and Y. Miyahara, 'Potential behavior of biochemically modified gold electrode for extended-gate field-effect transistor,' Japanese Journal of Applied Physics, vol. 44, p. 2860, 2005. [12] L.-T. Yin, J.-C. Chou, W.-Y. Chung, T.-P. Sun, and S.-K. Hsiung, 'Study of indium tin oxide thin film for separative extended gate ISFET,' Materials Chemistry and Physics, vol. 70, pp. 12-16, 2001. [13] J. Jung, S. J. Kim, D. H. Yoon, B. Kim, S. H. Park, and H. J. Kim, 'Electrical Responses of Artificial DNA Nanostructures on Solution-Processed In-Ga-Zn-O Thin-Film Transistors with Multistacked Active Layers,' ACS applied materials & interfaces, vol. 5, pp. 98-102, 2012. [14] S. J. Kim, J. Jung, K. W. Lee, D. H. Yoon, T. S. Jung, S. R. Dugasani, et al., 'Low-cost label-free electrical detection of artificial DNA nanostructures using solution-processed oxide thin-film transistors,' ACS applied materials & interfaces, vol. 5, pp. 10715-10720, 2013. [15] S. J. Kim, J. Jung, D. H. Yoon, and H. J. Kim, 'The effect of various solvents on the back channel of solution-processed In–Ga–Zn–O thin-film transistors intended for biosensor applications,' Journal of Physics D: Applied Physics, vol. 46, p. 035102, 2013. [16] J. S. Park, W.-J. Maeng, H.-S. Kim, and J.-S. Park, 'Review of recent developments in amorphous oxide semiconductor thin-film transistor devices,' Thin Solid Films, vol. 520, pp. 1679-1693, 2012. [17] M. Ito, C. Miyazaki, M. Ishizaki, M. Kon, N. Ikeda, T. Okubo, et al., 'Application of amorphous oxide TFT to electrophoretic display,' Journal of Non-Crystalline Solids, vol. 354, pp. 2777-2782, 2008. [18] Y.-C. Shen, C.-H. Yang, S.-W. Chen, S.-H. Wu, T.-L. Yang, and J.-J. Huang, 'IGZO thin film transistor biosensors functionalized with ZnO nanorods and antibodies,' Biosensors and Bioelectronics, vol. 54, pp. 306-310, 2014. [19] K. H. Ji, J.-I. Kim, Y.-G. Mo, J. H. Jeong, S. Yang, C.-S. Hwang, et al., 'Comparative Study on Light-Induced Bias Stress Instability of IGZO Transistors With and Gate Dielectrics,' Electron Device Letters, IEEE, vol. 31, pp. 1404-1406, 2010. [20] H. Lorenz, M. Despont, N. Fahrni, N. LaBianca, P. Renaud, and P. Vettiger, 'SU-8: a low-cost negative resist for MEMS,' Journal of Micromechanics and Microengineering, vol. 7, p. 121, 1997. [21] J. L. West and N. J. Halas, 'Applications of nanotechnology to biotechnology: Commentary,' Current opinion in Biotechnology, vol. 11, pp. 215-217, 2000. [22] K. Sokolov, M. Follen, J. Aaron, I. Pavlova, A. Malpica, R. Lotan, et al., 'Real-time vital optical imaging of precancer using anti-epidermal growth factor receptor antibodies conjugated to gold nanoparticles,' Cancer research, vol. 63, pp. 1999-2004, 2003. [23] X.-L. Hu, Y.-J. Zhu, and S.-W. Wang, 'Sonochemical and microwave-assisted synthesis of linked single-crystalline ZnO rods,' Materials Chemistry and Physics, vol. 88, pp. 421-426, 12/15/ 2004. [24] X. Chen, G. Cheng, and S. Dong, 'Amperometric tyrosinase biosensor based on a sol–gel-derived titanium oxide–copolymer composite matrix for detection of phenolic compounds,' Analyst, vol. 126, pp. 1728-1732, 2001. [25] F. Majone, S. Tonetto, C. Soligo, and M. Panozzo, 'Identification of kinetochores and DNA synthesis in micronuclei induced by mitomycin C and colchicine in Chinese hamster ovary cells,' Teratogenesis, carcinogenesis, and mutagenesis, vol. 12, pp. 155-166, 1992. [26] L.-S. Jang and H.-K. Keng, 'Modified fabrication process of protein chips using a short-chain self-assembled monolayer,' Biomedical microdevices, vol. 10, pp. 203-211, 2008. [27] P. Skehan, R. Storeng, D. Scudiero, A. Monks, J. McMahon, D. Vistica, et al., 'New colorimetric cytotoxicity assay for anticancer-drug screening,' Journal of the National Cancer Institute, vol. 82, pp. 1107-1112, 1990. [28] S. Ghaemmaghami, W.-K. Huh, K. Bower, R. W. Howson, A. Belle, N. Dephoure, et al., 'Global analysis of protein expression in yeast,' Nature, vol. 425, pp. 737-741, 2003. [29] E. Stenberg, B. Persson, H. Roos, and C. Urbaniczky, 'Quantitative determination of surface concentration of protein with surface plasmon resonance using radiolabeled proteins,' Journal of colloid and interface science, vol. 143, pp. 513-526, 1991. [30] Z. Zhong, M. Li, D. Xiang, N. Dai, Y. Qing, D. Wang, et al., 'Signal amplification of electrochemical immunosensor for the detection of human serum IgG using double-codified nanosilica particles as labels,' Biosensors and Bioelectronics, vol. 24, pp. 2246-2249, 2009. [31] K. Abe, K. Takahashi, A. Sato, H. Kumomi, K. Nomura, T. Kamiya, et al., 'Amorphous In–Ga–Zn–O Dual-Gate TFTs: Current–Voltage Characteristics and Electrical Stress Instabilities,' Electron Devices, IEEE Transactions on, vol. 59, pp. 1928-1935, 2012. [32] K.-S. Son, J.-S. Jung, K.-H. Lee, T.-S. Kim, J.-S. Park, Y.-H. Choi, et al., 'Characteristics of double-gate Ga–In–Zn–O thin-film transistor,' Electron Device Letters, IEEE, vol. 31, pp. 219-221, 2010. [33] K. Weber and M. Osborn, 'The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis,' Journal of Biological Chemistry, vol. 244, pp. 4406-4412, 1969. [34] H. Schagger and G. Von Jagow, 'Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa,' Analytical biochemistry, vol. 166, pp. 368-379, 1987. [35] J. Adams, 'Technical considerations on the use of horseradish peroxidase as a neuronal marker,' Neuroscience, vol. 2, pp. 141-145, 1977. [36] W. N. Burnette, '“Western blotting”: electrophoretic transfer of proteins from sodium dodecyl sulfate-polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A,' Analytical biochemistry, vol. 112, pp. 195-203, 1981. [37] G. R. Pearson, 'ELISA tests and monoclonal antibodies for EBV,' Journal of virological methods, vol. 21, pp. 97-104, 1988. [38] J. Homola, S. S. Yee, and G. Gauglitz, 'Surface plasmon resonance sensors: review,' Sensors and Actuators B: Chemical, vol. 54, pp. 3-15, 1999. [39] J. Homola, 'Present and future of surface plasmon resonance biosensors,' Analytical and bioanalytical chemistry, vol. 377, pp. 528-539, 2003. [40] C.-C. Chang, N.-F. Chiu, D. S. Lin, Y. Chu-Su, Y.-H. Liang, and C.-W. Lin, 'High-sensitivity detection of carbohydrate antigen 15-3 using a gold/zinc oxide thin film surface plasmon resonance-based biosensor,' Analytical chemistry, vol. 82, pp. 1207-1212, 2010. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/56967 | - |
| dc.description.abstract | 在這篇論文中，我們以氧化銦鎵鋅薄膜電晶體並附有延伸金感測板構成生醫感測器檢測蛋白質。電晶體扮演著感應與訊號輸出的角色，而金感測板具有傳導蛋白質電荷到電晶體通道層以改變通道電流的功能。我們在此研究中測試了兩種蛋白質，一種則是小鼠血清裡的免疫球蛋白G，一種是人類皰疹病毒第四型(EBV)的抗體。在免疫球蛋白G的檢測中，我們利用氧化鋅奈米柱對感應區表面作改質，並藉由其高面積對體積比例而感善了感測器的靈敏度。在人類泡疹病毒第四型的檢測中，我們利用自組裝分子層及蛋白質交聯劑來作改質，為的是改善蛋白質的固定化。進一步地，我們還運用了上閘極電壓變化的分析來增加實驗的可靠性與可重複性。最後，我們將生醫感測器與目前現有的蛋白質檢測方法做比較，省時、高敏感度是我們感測器的顯著優勢。我們可以在10pg/ml離體的小鼠血清溶液中量測到濃度為8.98fM的小鼠免疫球蛋白G。並且專一性地量測到濃度為10pg/ml的EBV抗體溶液。 | zh_TW |
| dc.description.abstract | A biosensor structure consisting of an Indium-Gallium-Zinc-Oxide (IGZO) thin film transistor (TFT) and a gold extended sensing pad was demonstrated. The IGZO-TFT was the device for signal extraction, and the sensing pad could conduct protein charges resulting channel current changed. There were two kinds of target protein in the thesis, mouse IgG in the mouse serum and Epstein-Barr virus antibodies. In the detection for mouse IgG, the biosensor was functionalized by applying ZnO nanorods on the pad to modify the gold surface. ZnO nanorods improved the sensitivity of the biosensors by high surface-to-volume ratio. In the detection for EBV antibodies, the device was functionalized by self-assembled monolayer and crosslinker to solve the problem of protein immobilization. Furthermore, the top gate voltage analysis was employed to improve reliability and repeatability of the experiment. Finally, the TFT-biosensor was compared with the conventional methods for protein detection. Time-saving and high sensitivity were the significant advantages of the TFT biosensor. The bio-TFT is able to selectively detect 10pg/ml mouse IgG in the serum solution and 10pg/ml EBV antibodies in the buffer solution. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-16T06:32:04Z (GMT). No. of bitstreams: 1 ntu-103-R01941094-1.pdf: 7342417 bytes, checksum: 6e712e2f6c96c529a0df833ad26c0ac0 (MD5) Previous issue date: 2014 | en |
| dc.description.tableofcontents | 謝誌 I
摘要 II Abstract III Contents IV List of Figure VI List of Table IX Chapter 1 Introduction 1 1.1 Background of Epstein-Barr virus detection 1 1.2 Overview of FET biosensors 4 1.3 Thesis structure 7 Chapter 2 Fabrication of the TFT-biosensors 8 2.1 Introduction 8 2.2 Structure and fabrication 9 2.3 Functionalization by ZnO nanorods 13 2.4 Functionalization by SAM and crosslinker 21 Chapter 3 Protein detection of IgG in mouse serum 23 3.1 Introduction 23 3.2 Quantification and analysis of the ZnO nanorods 24 3.3 Detection IgG in mouse serum by TFT-biosensors 27 Chapter 4 Protein detection of Epstein-Barr Virus 36 4.1 Introduction 36 4.2 Detection EBV antibodies in buffer by TFT-biosensors 37 4.4 Comparison of conventional methods 52 4.5 Summary 62 Chapter 5 Conclusion 63 Reference 64 | |
| dc.language.iso | en | |
| dc.subject | 小鼠免疫球蛋白G | zh_TW |
| dc.subject | 人類皰疹病毒第四型 | zh_TW |
| dc.subject | 薄膜電晶體 | zh_TW |
| dc.subject | 高敏感度生物感測器 | zh_TW |
| dc.subject | 蛋白質檢測 | zh_TW |
| dc.subject | Epstein-Barr virus | en |
| dc.subject | mouse IgG | en |
| dc.subject | protein detection | en |
| dc.subject | high sensitivity biosensor | en |
| dc.subject | TFT | en |
| dc.title | 高敏感度銦鎵鋅氧化物薄膜電晶體於EB病毒蛋白質檢測應用 | zh_TW |
| dc.title | High Sensitivity IGZO Thin Film Transistor Biosensors for the Detection of Epstein-Barr Virus Protein | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 102-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.coadvisor | 黃建璋(JianJang Huang) | |
| dc.contributor.oralexamcommittee | 楊宗霖(Tsung-Lin Yang),吳育任(Yuh-Renn Wu) | |
| dc.subject.keyword | 小鼠免疫球蛋白G,人類皰疹病毒第四型,薄膜電晶體,高敏感度生物感測器,蛋白質檢測, | zh_TW |
| dc.subject.keyword | mouse IgG,Epstein-Barr virus,TFT,high sensitivity biosensor,protein detection, | en |
| dc.relation.page | 67 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2014-08-06 | |
| dc.contributor.author-college | 電機資訊學院 | zh_TW |
| dc.contributor.author-dept | 光電工程學研究所 | zh_TW |
| 顯示於系所單位： | 光電工程學研究所 | |
文件中的檔案：
| 檔案 | 大小 | 格式 | |
|---|---|---|---|
| ntu-103-1.pdf 未授權公開取用 | 7.17 MB | Adobe PDF |
系統中的文件，除了特別指名其著作權條款之外，均受到著作權保護，並且保留所有的權利。