請用此 Handle URI 來引用此文件：
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/56842完整後設資料紀錄
| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 林峰輝(Feng-Huei Lin) | |
| dc.contributor.author | Yen-Yu Chen | en |
| dc.contributor.author | 陳彥宇 | zh_TW |
| dc.date.accessioned | 2021-06-16T05:51:59Z | - |
| dc.date.available | 2019-09-04 | |
| dc.date.copyright | 2014-09-04 | |
| dc.date.issued | 2014 | |
| dc.date.submitted | 2014-08-08 | |
| dc.identifier.citation | [1] Kershaw EE, Flier JS. Adipose tissue as an endocrine organ. The Journal of clinical endocrinology and metabolism. 2004;89:2548-56.
[2] Sommer B, Sattler G. Current concepts of fat graft survival: histology of aspirated adipose tissue and review of the literature. Dermatologic surgery : official publication for American Society for Dermatologic Surgery [et al]. 2000;26:1159-66. [3] Tam CS, Lecoultre V, Ravussin E. Brown adipose tissue: mechanisms and potential therapeutic targets. Circulation. 2012;125:2782-91. [4] Nishimura S, Manabe I, Nagasaki M, Hosoya Y, Yamashita H, Fujita H, et al. Adipogenesis in obesity requires close interplay between differentiating adipocytes, stromal cells, and blood vessels. Diabetes. 2007;56:1517-26. [5] Patrick CW. Breast tissue engineering. Annual review of biomedical engineering. 2004;6:109-30. [6] Ramsay DT, Mitoulas LR, Kent JC, Larsson M, Hartmann PE. The use of ultrasound to characterize milk ejection in women using an electric breast pump. Journal of human lactation : official journal of International Lactation Consultant Association. 2005;21:421-8. [7] Lee MC, Rogers K, Griffith K, Diehl KA, Breslin TM, Cimmino VM, et al. Determinants of breast conservation rates: reasons for mastectomy at a comprehensive cancer center. The breast journal. 2009;15:34-40. [8] Murphy RX. American Society of Plastic Surgeons 2013 Plastic Surgery Statistics Report. ASPS National Clearinghouse of Plastic Surgery Procedural Statistics2013. p. 22. [9] Argus A, Mahoney MC. Indications for breast MRI: case-based review. AJR American journal of roentgenology. 2011;196:WS1-14. [10] Robbins TH. Rectus abdominis myocutaneous flap for breast reconstruction. The Australian and New Zealand journal of surgery. 1979;49:527-30. [11] Futter CM, Webster MH, Hagen S, Mitchell SL. A retrospective comparison of abdominal muscle strength following breast reconstruction with a free TRAM or DIEP flap. British journal of plastic surgery. 2000;53:578-83. [12] Pacella SJ, Vogel JE, Locke MB, Codner MA. Aesthetic and technical refinements in latissimus dorsi implant breast reconstruction: a 15-year experience. Aesthetic surgery journal / the American Society for Aesthetic Plastic surgery. 2011;31:190-9. [13] Di Candia M, Asfoor AA, Jessop ZM, Kumiponjera D, Hsieh F, Malata CM. Previous multiple abdominal surgeries: a valid contraindication to abdominal free flap breast reconstruction? Eplasty. 2012;12:e31. [14] Peer L. Loss of Weight and Volume in Human Fat Grafts: with Postulation of a 'Cell Survival Theory'. Plastic & Reconstructive Surgery. 1950;5:217-30. [15] Tanzi MC, Fare S. Adipose tissue engineering: state of the art, recent advances and innovative approaches. Expert review of medical devices. 2009;6:533-51. [16] Rajesh RV, Heo GN, Park MR, Nam JS, Kim NK, Yoon D, et al. Proteomic analysis of bovine omental, subcutaneous and intramuscular preadipocytes during in vitro adipogenic differentiation. Comparative biochemistry and physiology Part D, Genomics & proteomics. 2010;5:234-44. [17] Kim D, Monaco E, Maki A, de Lima AS, Kong HJ, Hurley WL, et al. Morphologic and transcriptomic comparison of adipose- and bone-marrow-derived porcine stem cells cultured in alginate hydrogels. Cell and tissue research. 2010;341:359-70. [18] Patrick CW, Jr. Adipose tissue engineering: the future of breast and soft tissue reconstruction following tumor resection. Seminars in surgical oncology. 2000;19:302-11. [19] Chen GP, Ushida T, Tateishi T. Scaffold design for tissue engineering. Macromol Biosci. 2002;2:67-77. [20] Rezwan K, Chen QZ, Blaker JJ, Boccaccini AR. Biodegradable and bioactive porous polymer/inorganic composite scaffolds for bone tissue engineering. Biomaterials. 2006;27:3413-31. [21] Patrick CW, Jr., Chauvin PB, Hobley J, Reece GP. Preadipocyte seeded PLGA scaffolds for adipose tissue engineering. Tissue engineering. 1999;5:139-51. [22] Patrick CW, Jr. Tissue engineering strategies for adipose tissue repair. The Anatomical record. 2001;263:361-6. [23] Augst AD, Kong HJ, Mooney DJ. Alginate hydrogels as biomaterials. Macromol Biosci. 2006;6:623-33. [24] Hohenester E, Tisi D, Talts JF, Timpl R. The crystal structure of a laminin G-like module reveals the molecular basis of alpha-dystroglycan binding to laminins, perlecan, and agrin. Molecular cell. 1999;4:783-92. [25] Garrett RH, Grisham CM. BIOCHEMISTRY. 4 ed: brooks/cole cengage learning; 2010. [26] Halberstadt C, Austin C, Rowley J, Culberson C, Loebsack A, Wyatt S, et al. A hydrogel material for plastic and reconstructive applications injected into the subcutaneous space of a sheep. Tissue engineering. 2002;8:309-19. [27] Chandler EM, Berglund CM, Lee JS, Polacheck WJ, Gleghorn JP, Kirby BJ, et al. Stiffness of photocrosslinked RGD-alginate gels regulates adipose progenitor cell behavior. Biotechnology and bioengineering. 2011;108:1683-92. [28] Yoon DM, Curtiss S, Reddi AH, Fisher JP. Addition of hyaluronic acid to alginate embedded chondrocytes interferes with insulin-like growth factor-1 signaling in vitro and in vivo. Tissue engineering Part A. 2009;15:3449-59. [29] Scorr JE, nOWELL MJ. ON THE MECHANISM OF SCISSION OF ALGINATE CHAINS BY PERIODATE. Carbohydrate Research. 1976;47:105-17. [30] Kang HA, Shin MS, Lee MY, Yang JW. Effectiveness test of alginate-derived polymeric surfactants. Abstr Pap Am Chem S. 2001;222:U447-U. [31] Su WY, Chen KH, Chen YC, Lee YH, Tseng CL, Lin FH. An injectable oxidated hyaluronic acid/adipic acid dihydrazide hydrogel as a vitreous substitute. Journal of biomaterials science Polymer edition. 2011;22:1777-97. [32] McMurry J. Organic Chemistry. 7 ed: brooks/cole cengage learning; 2008. [33] Singh M, Ray AR, Vasudevan P. Biodegradation studies on periodate oxidized cellulose. Biomaterials. 1982;3:16-20. [34] Place ES, Rojo L, Gentleman E, Sardinha JP, Stevens MM. Strontium- and zinc-alginate hydrogels for bone tissue engineering. Tissue engineering Part A. 2011;17:2713-22. [35] Ribeiro CC, Barrias CC, Barbosa MA. Calcium phosphate-alginate microspheres as enzyme delivery matrices. Biomaterials. 2004;25:4363-73. [36] Boontheekul T, Kong HJ, Mooney DJ. Controlling alginate gel degradation utilizing partial oxidation and bimodal molecular weight distribution. Biomaterials. 2005;26:2455-65. [37] Chen F, Tian M, Zhang DM, Wang JY, Wang QG, Yu XX, et al. Preparation and characterization of oxidized alginate covalently cross-linked galactosylated chitosan scaffold for liver tissue engineering. Mat Sci Eng C-Mater. 2012;32:310-20. [38] Bryant SJ, Durand KL, Anseth KS. Degradation kinetics influence ECM production of photoencapsulated chondrocytes in PEG-based hydrogels. Abstr Pap Am Chem S. 2001;222:U244-U. [39] Bryant SJ, Anseth KS. Hydrogel properties influence ECM production by chondrocytes photoencapsulated in poly(ethylene glycol) hydrogels. Journal of biomedical materials research. 2001;59:63-72. [40] Tampieri A, Sandri M, Landi E, Celotti G, Roveri N, Mattioli-Belmonte M, et al. HA/alginate hybrid composites prepared through bio-inspired nucleation. Acta biomaterialia. 2005;1:343-51. [41] Burdick JA, Chung C, Jia X, Randolph MA, Langer R. Controlled degradation and mechanical behavior of photopolymerized hyaluronic acid networks. Biomacromolecules. 2005;6:386-91. [42] Kwon YJ, Peng CA. Calcium-alginate gel bead cross-linked with gelatin as microcarrier for anchorage-dependent cell culture. BioTechniques. 2002;33:212-4, 6, 8. [43] Jeon O, Powell C, Ahmed SM, Alsberg E. Biodegradable, photocrosslinked alginate hydrogels with independently tailorable physical properties and cell adhesivity. Tissue engineering Part A. 2010;16:2915-25. [44] Jing W, Lin Y, Wu L, Li X, Nie X, Liu L, et al. Ectopic adipogenesis of preconditioned adipose-derived stromal cells in an alginate system. Cell and tissue research. 2007;330:567-72. [45] Tan H, Chu CR, Payne KA, Marra KG. Injectable in situ forming biodegradable chitosan-hyaluronic acid based hydrogels for cartilage tissue engineering. Biomaterials. 2009;30:2499-506. [46] Mathur AM, Moorjani SK, Scranton AB. Methods for synthesis of hydrogel networks: A review. J Macromol Sci R M C. 1996;C36:405-30. [47] Matsumoto T, Kano K, Kondo D, Fukuda N, Iribe Y, Tanaka N, et al. Mature adipocyte-derived dedifferentiated fat cells exhibit multilineage potential. Journal of cellular physiology. 2008;215:210-22. [48] Jones JR, Barrick C, Kim KA, Lindner J, Blondeau B, Fujimoto Y, et al. Deletion of PPARgamma in adipose tissues of mice protects against high fat diet-induced obesity and insulin resistance. Proceedings of the National Academy of Sciences of the United States of America. 2005;102:6207-12. [49] Wang Y, Sul HS. Pref-1 regulates mesenchymal cell commitment and differentiation through Sox9. Cell metabolism. 2009;9:287-302. [50] Mitterberger MC, Lechner S, Mattesich M, Kaiser A, Probst D, Wenger N, et al. DLK1(PREF1) is a negative regulator of adipogenesis in CD105(+)/CD90(+)/CD34(+)/CD31(-)/FABP4(-) adipose-derived stromal cells from subcutaneous abdominal fat pats of adult women. Stem cell research. 2012;9:35-48. [51] Gregoire FM, Smas CM, Sul HS. Understanding adipocyte differentiation. Physiological reviews. 1998;78:783-809. [52] Uriel S, Huang JJ, Moya ML, Francis ME, Wang R, Chang SY, et al. The role of adipose protein derived hydrogels in adipogenesis. Biomaterials. 2008;29:3712-9. [53] Kim WS, Mooney DJ, Arany PR, Lee K, Huebsch N, Kim J. Adipose tissue engineering using injectable, oxidized alginate hydrogels. Tissue engineering Part A. 2012;18:737-43. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/56842 | - |
| dc.description.abstract | 目前臨床上常使用自體脂肪移植的方式進行乳房重建手術，直接抽脂後注入乳房中，雖然具有相當多優點，但仍有40至60 %的體積會在植入後鈣化或脂肪再吸收，造成患者需要重覆手術才能維持理想的體積。本研究提出一種新材料－－透明質酸-層粘連蛋白-褐藻酸，能夠做為脂肪組織工程的細胞載體，應用於乳房重建手術中可以改善現行臨床上脂肪再吸收的問題。研究結果顯示，透明質酸-層粘連蛋白-褐藻酸能夠製作出體積約1.886±0.07 mm3的微球細胞載體，具有良好的質傳效率，降解至第六十天仍有60 %的質量，並且材料本身不具毒性，能夠促進細胞貼附、增生及維持分化，在第二週時不論是細胞活性或是維持分化能力都顯著高於一般的褐藻酸載體。於動物體內試驗也證明，透明質酸-層粘連蛋白-褐藻酸能夠同時促進脂肪分化及血管新生，避免脂肪再吸收及鈣化，甚至第三週時能再增加42 %的體積，證明透明質酸-層粘連蛋白-褐藻酸相當具有潛力應用於脂肪組織工程及改善乳房重建手術。 | zh_TW |
| dc.description.abstract | In recent years, autologous fat transplantation is commonly used in clinical breast reconstruction surgery. Fat tissue injected into the breast directly after liposuction, it has many benefits, however, tissue resorption or calcification result in 40–60% of the graft volume loss. It cause patient must accept reoperation for maintain identical volume. In current study present a new material-- Hyaluronic acid-Laminin-Alginate as a cell carrier for used in adipose tissue engineering and improve breast reconstruction surgery by avoid tissue resorption. Results showed that Hyaluronic acid-Laminin-Alginate could create a 1.886±0.07 mm3 cell carrier, has a suitable mass transfer efficiency. Also, it could remain 60 % weight after 60 days degraded, non-cytotoxicity, could promote cells attachment, proliferation and differentiation. Hyaluronic acid-Laminin-Alginate has significantly higher cell proliferation and adipogenesis than alginate in 2 weeks data. In vivo animal study also prove that Hyaluronic acid-Laminin-Alginate could promote both adipogenesis and angiogenesis, prevent resorption and calcification, and increase 42 % volume after 3 weeks implanted. Hyaluronic acid-Laminin-Alginate has the potential to be applied in adipose tissue engineering and improve breast reconstruction surgery. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-16T05:51:59Z (GMT). No. of bitstreams: 1 ntu-103-R01548012-1.pdf: 4506255 bytes, checksum: f2dde933404a90b05ac179ba97d15133 (MD5) Previous issue date: 2014 | en |
| dc.description.tableofcontents | 誌謝 ii
中文摘要 vii ABSTRACT viii 目次 ix 圖目次 xiii 表目次 xv 第一章 緒論 1 1.1 脂肪組織 1 1.1.1 脂肪細胞 2 1.1.2 其他細胞 4 1.2 乳房 5 1.3 全乳房切除術 6 1.4 乳房重建 7 1.4.1 人工植入物 8 1.4.2 自體組織移植 9 1.4.3 自體脂肪移植 11 1.5 細胞治療 12 1.5.1 成熟脂肪細胞 12 1.5.2 脂肪前驅細胞 12 1.5.3 脂肪幹細胞 13 1.6 訊息因子 15 1.7 支架 16 1.7.1 海綿狀 16 1.7.2 微球狀 16 1.8 褐藻酸 17 1.9 材料改質 18 1.9.1 層黏連蛋白 18 1.9.2 透明質酸 19 1.10 研究目的 20 第二章 理論基礎 21 2.1 RGD序列修飾之褐藻酸 21 2.2 透明質酸摻混褐藻酸 23 2.3 褐藻酸氧化開環 24 2.4 透明質酸氧化開環 25 2.5 亞胺形成反應 26 第三章 材料與方法 27 3.1 材料 27 3.2 透明質酸-層粘連蛋白-褐藻酸製備 29 3.3 脂肪幹細胞萃取 32 3.4 誘導脂肪分化 34 3.5 材料分析 35 3.4.1 傅里葉轉換紅外光譜 35 3.4.2 降解試驗 35 3.6 生物相容性試驗 37 3.5.1 3T3-L1脂肪前驅細胞株培養 37 3.5.2 乳酸脫氫酶細胞毒性試驗 37 3.5.3 存活/死亡細胞染色 38 3.5.4 WST-1細胞活性分析 38 3.5.5 掃描式電子顯微鏡觀察 39 3.7 脂肪分化評估試驗 40 3.6.1 流式細胞儀分析 40 3.6.2 免疫螢光染色分析 40 3.6.3 油紅染色分析 41 3.6.4 脂肪分化定量分析 41 3.8 體內試驗 42 3.7.1 小鼠皮下植入試驗 42 3.7.2 小動物電腦斷層掃描分析 42 3.7.3 組織學染色分析 42 3.9 統計分析 43 第四章 結果與討論 44 4.1 材料分析結果 44 4.1.1 HA-L-Alg材料合成之驗證 45 4.2 生物相容性試驗結果 49 4.2.1 細胞毒性測試結果 49 4.2.2 細胞死亡/存活染色結果 50 4.3 脂肪細胞包覆於材料之測試結果 54 4.3.1 包覆於材料中之脂肪細胞活性 54 4.3.2 脂肪細胞包覆於微球中之影像 55 4.4 脂肪分化評估結果 58 4.4.1 流式細胞儀測試結果 58 4.4.2 免疫螢光染色測試結果 60 4.4.3 油紅染色測試結果 61 4.5 脂肪分化結果比較 63 4.6 動物體內試驗結果 65 4.6.1 小動物電腦斷層掃描 65 4.6.2 植入物取出後比較 67 4.6.3 組織學染色結果 68 第五章 結論 70 參考文獻 72 | |
| dc.language.iso | zh-TW | |
| dc.subject | 支架 | zh_TW |
| dc.subject | 乳房重建 | zh_TW |
| dc.subject | 脂肪組織工程 | zh_TW |
| dc.subject | Adipose tissue engineering | en |
| dc.subject | Breast reconstruction | en |
| dc.subject | Scaffold | en |
| dc.title | 透明質酸-層黏連蛋白-褐藻酸細胞載體應用於脂肪組織工程之研究 | zh_TW |
| dc.title | The Study of HA-Laminin-Alginate Bead as Cell Carrier for Adipose Tissue Engineering | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 102-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.coadvisor | 戴浩志(Hao-Chih Tai) | |
| dc.contributor.oralexamcommittee | 徐善慧(Shan-hui Hsu),楊禎明(Jen-Ming Yang),張國基(Kuo-Chi Chang) | |
| dc.subject.keyword | 脂肪組織工程,支架,乳房重建, | zh_TW |
| dc.subject.keyword | Adipose tissue engineering,Scaffold,Breast reconstruction, | en |
| dc.relation.page | 77 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2014-08-08 | |
| dc.contributor.author-college | 工學院 | zh_TW |
| dc.contributor.author-dept | 醫學工程學研究所 | zh_TW |
| 顯示於系所單位： | 醫學工程學研究所 | |
文件中的檔案：
| 檔案 | 大小 | 格式 | |
|---|---|---|---|
| ntu-103-1.pdf 未授權公開取用 | 4.4 MB | Adobe PDF |
系統中的文件，除了特別指名其著作權條款之外，均受到著作權保護，並且保留所有的權利。