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| ???org.dspace.app.webui.jsptag.ItemTag.dcfield??? | Value | Language |
|---|---|---|
| dc.contributor.advisor | 杜宜殷(Yi-Yin Do) | |
| dc.contributor.author | Chien-Hsiang Chiu | en |
| dc.contributor.author | 邱建翔 | zh_TW |
| dc.date.accessioned | 2021-06-16T05:41:03Z | - |
| dc.date.available | 2019-09-04 | |
| dc.date.copyright | 2014-09-04 | |
| dc.date.issued | 2014 | |
| dc.date.submitted | 2014-08-12 | |
| dc.identifier.citation | 李宜龍. 2008. 苦瓜與蝴蝶蘭EIN3同源基因選殖與分析. 國立臺灣大學園藝學系碩士論文.
李盛新. 2006. 香蕉 ACC 氧化酶基因啟動子活性分析與默化質體之轉殖. 國立臺灣大學園藝學系碩士論文. 林宜佑. 2004. 應用RNA干擾技術抑制香蕉ACC氧化酶基因表現之研究. 國立臺灣大學園藝學系碩士論文. 葉美倫. 2010. 香蕉ACC氧化酶基因默化轉殖株分析. 國立臺灣大學園藝學系碩士論文. 廖育辰. 2008. 香蕉ACC氧化酶基因默化轉殖之研究. 國立台灣大學園藝學系碩士論文. Abeles, F. B. 1973. Ethylene in plant biology. Academic Press, London. 302pp. Areas J.A.G., F. M. Lajolo. 1981. Starch transformation during banana ripening: I -The phosphorylase and phosphatase behaviour in Musa acuminata. J. Food Biochem. 5: 19-37 Baroja-Fernandez, E., F. J. Munoz, J. Li, A. Bahaji, G. Almargo, M. Montero, E. Etxeberria, M. Hidalgo, M. T. Sesma, an J. Pozueta-Romero. 2012. Sucrose synthase activity in the sus1/sus2/sus3/sus4 Arabidopsis mutant is sufficient to support normal cellulose and starch production. Proc. Natl. Acad. Sci. 109: 321-326. Barratt D.H.P., et al. 2009. Normal growth of Arabidopsis requires cytosolic invertase but not sucrose synthase. Proc. Natl. Acad. Sci. USA 106: 13124–13129. Barry, C. S., B. Blume, M. Bouzayen, W. Cooper, A. J. Hamilton, and D. Grierson. 1996. Differertial expression of the 1-aminocyclopropane-1-carboxylate oxidase gene family of tomaoto. Plant J. 9:525-535. Baier M, Hemmann G, Holman R, Corke F, Card R, Smith C, Rook F, Bevan MW. 2004. Characterization of mutants in Arabidopsis showing increased sugar-specific gene expression, growth, and developmental responses. Plant Physiol . 134: 81–91 Boos, P. K., Davies, Christopher, and S. P. Robinson. 1996. Expression of anthocyanin biosynthesis pathways genes in red and white grapes. Plant J. 32: 565-569. Bramley, P. M. 2002. Regulation of carotenoid formation during tomato fruit ripening and development. J. Exp. Bot. 377: 2107-2113. Cabello, S., C. Lorenz, S. Crespo, J. Cabrera, R. Ludwing, C. Escobar, and J. Hofmann. 2014. Altered sucrose synthase and invertase expression affects the local and systemeric sugar metabolism of nematode-infected Arabidopsis thaliana plants. J. Exp. Bot. 2014. 65: 201-212. Chao, F., M. Chen, C. J. Xu, L. Bai, X. R. Yin, X. Li, A. C. Allan, L. B. Ferguson, and K. S. Chen. 2012. Transcriptomic analysis of Chinese bayberry(Myrica rubra) fruit development and ripening using RNA-Seq. BMC Genomics 13: 1471-2164. Choudhury, S. B., S. Roy, D. N. Sengupta. 2008. Characterization of transcriptional profiles of MA-ACS1 and MA-ACO1 genes in response to ethylene, auxin, wounding, cold and different photoperiods during ripening in banana fruit. J. Plant Physiol. 165: 1865-1878. Cordenunsi B.R., F. M. Lajolo. 1995. Starch breakdown during banana ripening: sucrose synthase and sucrose phosphate synthase. J. Agric. Food Chem. 43(2): 347-351 CSIRO. 1972. Banana ripening guide. Div Food Res., Cirular 8:1-12. do Nascimento, J. R. O., B. R. Cordenunsi, F. M. Lajojo, and M. J. C. Alcocer. 1997. Banana sucrose-phosphate synthase gene expression during fruit ripening. Planta 203: 283-288. Do, Y. Y., T. S. Thay, T.W. Chang, and P. L. Huang. 2005. Molecular cloning and characterization of a novel 1-aminocyclopropane-1carboxylate oxidase gene involved in ripening of banana fruits. J. Agric. Food Chem. 53:8239-8247. Dong, J. G., D. Olson, A. Silverstone, and S. F. Yang. 1992. Sequence of a cDNA coding for a 1-aminocyclopropane-1-carboxylate oxidase homolog from apple fruit. Plant Physiol. 98:1530-1531. Drury, R., S. Ho‥rtensteiner, I. Donnison, C. R. Bird, and G. B. Seymour. 1999. Chlorophyll catabolism and gene expression in the peel of ripening banana fruits. Physiol. Plant. 107: 32-38. Ehness, R., Ecker, M., Godt, D. E., and Roitsch, T. 1997. Glucose and stress independently regulate source and sink metabolism and defense mechanisms via signal transduction pathways involving protein phosphorylation. Plant Cell 9: 1825–1841. Feng, C., M. Chen, C. J. Xu, L. Bai, X. R. Y, X. Li, A. C. Allan, L. B. Ferguson, and K. S. Chen. 2012. Transcriptomic analysis of Chinese bayberry (Myrica rubra) fruit development and ripening using RNA-Seq. BMC Genomics 13:19. F. X. Cunningham, Jr. and E. Gantt. 1998. Genes and enzymes of carotenoid biosynthesis in plants. Plant Mol. Biol. 49: 557-583. Giovannoni. J. J. 2004. Genetic regulation of fruit development and ripening. Plant Cell 16: 170-180. Gollop, R., S. Even, V. Colova-Tsolova, A. Perl. 2002. Expression of the grape dihydroflavonol reductase gene and analysis of its promoter region. J. Exp. Bot. 53: 1397–1409 Gollop R, Farhi S, Peri A. 2001. Regulation of the leucoanthocyanidin dioxygenase gene expression in Vitis vinifera. Plant Sci. 161: 579–588. Grierson, D., A. Slater, J. Speirs, and G. A. Tucker. 1985. The appearance of polygalacturonase mRNA in tomatos; one of a series of changes in gene expression during development and ripening. Planta 163:263-271. Ha, S. H., J. B. Kim, J. S. Park, S. W. Lee, and K. J. Cho. 2007. A comparison of the carotenoid accumulation in Capsicum varieties that show different ripening colours: deletion of the capsanthin-capsorubin synthase gene is not a prerequisite for the formation of a yellow pepper. J. Exp. Bot. 58: 3135-3144. Hamilton, A. J., M. Bouzayen, and D. Grierson. 1991. Identification of a tomato for the ethylene-forming enzyme by expression in yeast. Proc. Natl. Acad. Sci. USA 88:7434-7437. He, Fei., L. Mu, G. L. Yan, N. N. Liang, Q. H. Pan, J. Wang, M. J. Reeves, an C. Q. Duan. 2010. Biosynthesis of anthocyanins and their Regulation in colored Grapes. Molecules 15: 9057-9091. Hont, A.D., F. Denoeud, J-M. Aury, F-C. Baurens, F. Carreel, O.Garsmeur, B.Noel, S.Bocs, G.Droc, M. Rouard, C.D.Silva, K.Jabbari, C.Cardi, J.Poulain, M.Souquet, K.Labadie, C.Jourda, J.Lengelle,M. Rodier-Goud,A. Alberti, M.Bernard, M.Correa, S.Ayyampalayam, M.R.Mckain, J.Leeben-Mack, D.Burgess8, M.Freeling8, D.Mbe’guie’-A-Mbe’guie’9, M.Chabannes5, T.Wicker, O.Panaud, J.Barbosa, E.Hribova, P.Heslop-Harrison, R.Habas5, R.Rivallan, P.Francois, C.Poiron, A.Kilian, D.Burthia, C.Jenny, F.Bakry, S.Brown, V.Guignon, G.Kema, M.Dita, C. Waalwijk, S.Joseph, A.Dievart , O.Jaillon, J.Leclercq, X.Argout, E.Lyons, A.Almeida8, M.Jeridi, J.Dolezel, N.Roux, A.M.Risterucci, J. Weissenbach, M.Ruiz, J.C.Glaszmann, F.Que’tier, N.Yahiaoui and P.Wincker. 2012. The banana (Musa acuminata) genome and the evolution of monocotyledonous plants. Nature 488:213-217. Huang, F. C., Y. Y. Do and P. L. Huang. 2006. Genomic organization of a diverse ACC synthase gene family in banana and expression characteristics of the gene member involved in ripening of banana fruits. J. Agric. Food Chem. 54: 3859-3868. Huang, P. L., J. E. Parks, W. H. Rottmann, and A. Theologis. 1991. Two genes encoding 1-aminocyclopropane-1-carboxylate synthase in zucchini (Cucurbita pepo) are clustered and similar but differentially regulated. Proc. Natl. Acad. Sci. 88: 7021-70215. Hubbard N.L., D. M. Pharr, S. C. Huber. 1990. Role of sucrose phosphate synthase in sucrose biosynthesis in ripening bananas and its relationship to the respiratory climacteric. Plant Physiol.94: 201-208. Hubert, O., G. Piral, C. Galas, F.-C. Baurens, D. Mbeguie-A-Mbeguie. 2014. Changes in ethylene signaling and MADS box gene expression areassociated with banana finger drop. Plant Sci. 223: 99-108. Jeong, S. T., N, Goto-Yamomoto, S. Kobayashi, M.Esaja. 2004. Effects of plant hormones and shading on the accumulation of anthocyanins and the expression of anthocyanin biosynthetic genes in grape berry skins. Plant Sci. 167: 247-252. Karlova R., F. M. Rosin, J. Busscher-Lange, V. Parapunova, P. T. Do, A. R. Fernie, P. D. Fraser, C. Baxter, G. C. Angenent, and R. A. deMaagd. 2011. Transcriptome and metabolite profiling show that APETALA2A is a major regulator of tomato fruit ripening. Plant cell 23: 923-941. Kitdamrongsont, K., P. Pothavorn, S. Swangpol, S. Wongniam, K. Atawongsa, J. Svasti, and J. Somana. 2008. Anthocyanin composition of wild bananas in Thailand. J. Agric. Food Chem. 56: 10853-10857. Koch K. 2004. Sucrose metabolism: regulatory mechanisms and pivotal roles in sugar sensing and plant development. Plant Biol. 7:235–246. Mita S, Hirano H, Nakamura K. 1997. Negative regulation in the expression of a sugar-inducible gene in Arabidopsis thaliana. Plant Physiol. 114: 575–582 Matzke. M., A. J. Matzke, and J. M. Kooter. 2001. RNA: guiding gene silencing. Science 293:1080-1083. Ne’meth K, Salchert K, Putnoky P, Bhalerao R, Koncz-Ka`lma`n Z, Stankovic-Stangeland B, Bako` L, Mathur J, O‥ kre’sz L, Stabel S, et al. 1998. Pleiotropic control of glucose response by PRL1, a nuclear WD protein, in Arabidopsis. Genes Dev. 12: 3059–3073 Oeller, P. W., M. W. Lu, L. P. Taylor, D. A. Pike, and A. Theologis. 1991. Reversible inhibition of tomato fruit senescence by antisense RNA. Science 254:437-439. Ohto M-A, K. Onai, Y. Furukawa, E. Aoki, T. Araki, K. Nakamura. 2001. Effects of sugar on vegetative development and floral transition in Arabidopsis. Plant Physiol. 127: 252–261 Sato, Y., R. Morita, M. Nishimura, H. Yamaguchi, and M. Kusaba. 2007. Mendel’s green cotyledon gene encodes a positive regulator of the chlorophyll-degrading pathway. PNAS 104: 14169-14174. Terra, N. N., E. Garcia, F. M. Lajolo. 1983. Starch sugar transformation during banana ripening.: the behavior of UDP-glucosepyrophosphorylase, sucrose synthetase and invertase. J. Food Sci. 48: 1097-1100. Tsukaya, H. T. Ohshima. S. Naito, M. Chino, Y. Chomeda. 1991. Sugar-dependent expression of CHS-A gene for chalcone synthase from petunia in transgenic Arabidopsis. Plant Physiol. 97: 1414-1421. Vargas W.A., G. L. Salerno. 2010. The Cinderella story of sucrose hydrolysis: alkaline/neutral invertases, from cyanobacteria to unforeseen roles in plant cytosol and organelles. Plant Sci. 178: 1–8. Wang, X., H. Kong, and H. Ma. 2014. F-box proteins regulate ethylene signaling and more. Genes Dev. 23: 391-396. Weiss D. 2000. Regulation of flower pigmentation and growth: multiple signaling pathways control anthocyanin synthesis in expanding petals. Physiol. Plant 110: 152–157 Wiersma, P. A., H. Zhang, C. Lu, A. Quail, and P. T. A. Toivonen. 2007. Survey of the expression of genes for ethylene synthesis and perception during maturation and ripening of ‘Sunrise’ and ‘Golden Delicious’ apple fruit. Postharvest Biol. Tec. 44: 204-211. Woodson. W. R., K. Y. Park, A. Drory, P. B. Larsen, and H. Wang. 1992. Expression of ethylene biosynthetic pathway transcript in senescing carnation flowers. Plant Physiol. 99:526-532. Worrell A.C., J. M. Bruneau, K. Summerfelt, M. Boersig, T. A. Voelker. 1991. Expression of a maize sucrose-phosphate synthase in tomatoes alters leaf carbohydrate partitioning. Plant Cell 3: 1121-1130. Xiong, A. S., Q. H. Yao, R. H. Peng, X. Li, P. L. Han, and H. Q. Fan. 2005. Different effects on ACC oxidase gene silencing triggered by RNA interference in transgenic tomato. Plant Cell 23:639-646. Yang, X. T., Z. Q. Zhang, D. J, X. M. Huang, L. Y. Xu, and X. Q. Pang. 2009. Charaterization of chlorophyll degradation in banana and plantain during ripening at high temperature. Food Chem. 114: 383-390. Yang, X. T., X. Q. Pang, L. Y. Xu, R. Fang, X. M. Huang, P. J. Guan, W. J. Lu, and Z. Zhang. 2009. Accumulation of soluble sugars in peel at high temperature leads to stay green ripe banana fruit. J. Exp. Bot. 40: 4051-4062. Yu, K., Q. Xu, X. Da, F. Guo, Y. Ding, and X. Deng. 2012. Transcriptome changes during fruit development and ripening of sweet orange (Citrus sinensis). BMC Genomics 13:10. Zhu, X., A. Wang, S. Zhu, and L. Zhang. 2011. Expression of ACO1, ERS1 and ERF1 genes in harvested bananas in relation toheat-induced defense against Colletotrichum musae. J. Plant Physiol. 168: 1634-1640. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/56668 | - |
| dc.description.abstract | 1-氨基環丙烷-1-羧酸 (1-aminocyclopropane-1-carboxylate acid, ACC) 氧化酶為參與植物荷爾蒙乙烯 (ethylene) 生合成的重要酵素。香蕉具有兩種ACC氧化酶，分別為 Mh-ACO1與Mh-ACO2，其酵素活性不同，為了瞭解此二種ACC氧化酶與香蕉果實後熟之相關性，本研究運用即時定量反轉錄聚合酶連鎖反應 (real-time reverse transcription polymerase chain reaction, real-time RT-PCR)，分別以北蕉Mh-ACO1默化轉殖株與Mh-ACO2默化轉殖株之果實為材料，進行乙烯生合成、乙烯訊息傳導、葉綠體代謝等相關基因之表現分析，結果顯示Mh-ACO1默化轉殖株中，Mh-ACO1基因之默化效果於果皮第五期尤為顯著，Mh-ACO2之基因表現也受到抑制，表明了Mh-ACO1與Mh-ACO2間存在互相調控的關係；於Mh-ACO2默化轉殖株中，Mh-ACO2最佳默化效果則出現在果皮第五至第六期。Mh-ACO1默化轉殖株果肉內，Mh-ACO1表現趨勢與未轉殖株完全相異，Mh-ACO1基因僅於第一期表現高於未轉殖株，之後呈現穩定表現，且未出現如未轉殖株之第三與第七期的表現高峰。Mh-ACO2基因於Mh-ACO2默化轉殖株果肉內第五期，具有良好之默化效果，但在第七期表現高於未轉殖株。於Mh-ACO1默化轉殖株果皮中，乙烯訊息傳導相關基因Mh-CTR1 (constitutive triple response 1) 具有不同於未轉殖株的表現趨勢，轉殖株於第五期具有顯著之基因表現高峰，而未轉殖株於第一至五期，維持低量表現。乙烯受體基因Mh-ERS1(ethylene response 1 )於Mh-ACO2默化轉殖株果肉內第四期，具有表現高峰，而未轉殖株之高峰於第五期才出現。葉綠素代謝基因CS2 (chlase 2) 於Mh-ACO2默化轉殖株與未轉殖株果皮七個時期，趨勢完全一致，但轉殖株之表現顯著低於未轉殖株。花青素生合成基因CHI (chalcone isomerase)，於Mh-ACO2默化轉殖株內之表現高峰，由未轉殖株的第三期延後至第六期。類胡蘿蔔素生合成基因ZDS1 (zeta-carotene 1) 於Mh-ACO1、Mh-ACO2默化轉殖株及未轉殖株表現相異，Mh-ACO1默化轉殖株內，ZDS1基因於第五期表現顯著高於未轉殖株，惟至第七期降為未轉殖株之一半表現量；於Mh-ACO2默化轉殖株內，ZDS1基因僅在果皮第三期出現略高於未轉殖株之表現高峰。 | zh_TW |
| dc.description.abstract | ACC oxidase is a crucial enzyme which participates in the ethylene biosynthesis of plants. Mh-ACO1 and Mh-ACO2 are two distinct ACC oxidase genes in bananas. To better understand the physiological functions of these two genes and the gene expression patterns that relate to ethylene biosynthesis and signal transduction, and chlorophyll metabolisms in ACC oxidase silenced transgenic banana fruits, the real-time RT-PCR was employed for gene expression analysis. The results showed that Mh-ACO1 gene had a significant silenced effect in the stage 5 of peels from Mh-ACO1 silenced transgenic banana, and the Mh-ACO2 gene was also repressed, indicating there existed an interaction between these two ACC oxidase genes. For the Mh-ACO2 silenced transgenic banana, the best silencing effect was shown in the stages 5 and 6 of peels. In addition, the gene expression pattern in pulps was quite different from untransformed wild type for the Mh-ACO1 gene in Mh-ACO1 silenced transgenic banana, and there was no gene expression peaks in stages 3 and 7 such as those in untransformed wild type, the Mh-ACO1 gene expressions in Mh-ACO1 silenced banana were than wild type in stages 3 and 7. The Mh-ACO2 gene expression level in pulps from Mh-ACO2 silenced transgenic banana showed the stage 5 had the most obvious silenced effect but the expression was higher than wild type in stage 7. Mh-CTR1, which is involved in ethylene signal transduction, showed a different gene expression tendency when in both Mh-ACO1 and Mh-ACO2 silenced transgenic bananas compared with wild type. The Mh-ACO1 silenced transgenic banana, expressed strongly Mh-CTR1 gene in stage 5 while the wild type retained a constant expression level. In the pulps of banana fruits, the expression of ethylene receptor gene Mh-ERS1 showed highest level in stage 4 in Mh-ACO2 silenced transgenic banana while highest level was found in stage 5 from untransformed wild type. The expression tendency of chlorophyll metabolism related gene CS2 was almost the same between Mh-ACO2 silenced transgenic banana and wild type, but the expression level for Mh-ACO2 silenced transgenic banana was quite low. The expression of CHI gene, involved in the anthocyanin biosynthesis pathway, was delayed from stages 3 to 6 in Mh-ACO2 silenced transgenic banana. As to the Carotenoid biosynthesis involved gene ZDS1, the expression patterns were totally different in Mh-ACO1, Mh-ACO2 silenced transgenic bananas, and the wild type. In the peels of Mh-ACO1 silenced transgenic banana, the ZDS1 expressed relatively high, but decreased in stage 7 where the expression level was half of the wild type. However, for ZDS1 gene in Mh-ACO2 silenced transgenic banana, the expression level was higher in stage 3. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-16T05:41:03Z (GMT). No. of bitstreams: 1 ntu-103-R01628127-1.pdf: 4389547 bytes, checksum: e32c27cec93a5d814361cda43efdea12 (MD5) Previous issue date: 2014 | en |
| dc.description.tableofcontents | 中文摘要 I
Abstract III 壹、前言 1 貳、前人研究 2 一、乙烯生合成相關基因之研究 2 二、乙烯訊息傳導相關基因之研究 3 三、葉綠素代謝相關基因研究 5 四、花青素生合成相關基因之研究 6 五、類胡蘿蔔素生合成相關基因之研究 7 六、醣類生合成相關基因研究 7 七、ACC氧化酶基因RNA干擾 (RNA interference, RNAi) 構築香蕉基因轉殖 8 八、ACC氧化酶基因默化香蕉轉殖株特性分析 8 九、轉錄體分析 11 參、材料與方法 14 一、植物材料 14 二、植物基因組RNA抽取 14 三、即時定量反轉錄聚合酶連鎖反應 14 肆、結果 22 一、ACC氧化酶基因默化轉殖株香蕉果皮內乙烯生合成相關基因表現 22 二、ACC氧化酶基因默化轉殖株香蕉果肉內乙烯生合成相關基因表現 22 三、ACC氧化酶基因默化轉殖株果皮內乙烯訊息傳導相關基因表現 22 四、ACC氧化酶基因默化轉殖株果肉內乙烯訊息傳導相關基因表現 23 五、ACC氧化酶基因默化轉殖株果皮內葉綠素代謝相關基因表現 24 六、ACC氧化酶基因默化轉殖株果皮內花青素生合成相關基因表現 24 七、ACC氧化酶基因默化轉殖株果皮內類胡蘿蔔素生合成相關基因表現 24 八、ACC氧化酶基因默化轉殖株果肉內醣類生合成相關基因表現 25 伍、討論 45 一、乙烯生合成相關基因於ACC氧化酶基因默化轉殖株不同後熟時期表現 45 二、乙烯訊息傳導相關基因於ACC氧化酶基因默化轉殖株不同後熟時期之表現 46 三、葉綠素代謝相關基因於ACC氧化酶基因默化轉殖株不同後熟時期之表現 46 四、花青素生合成相關基因於ACC氧化酶基因默化轉殖株不同後熟時期之表現 47 五、類胡蘿蔔素生合成相關基因於ACC氧化酶基因默化轉殖株不同後熟時期之表現 47 六、醣類生合成相關基因於ACC氧化酶基因默化轉殖株不同後熟時期之表現 48 七、熱圖譜分析轉殖株內基因表現形態 48 陸、結語 50 柒、參考文獻 51 | |
| dc.language.iso | zh-TW | |
| dc.subject | 果實後熟 | zh_TW |
| dc.subject | ACC氧化? | zh_TW |
| dc.subject | RNA干擾 | zh_TW |
| dc.subject | fruit ripening | en |
| dc.subject | RNA interference | en |
| dc.subject | ACC oxidase | en |
| dc.title | 香蕉ACC氧化酶基因默化轉殖株中果實後熟相關基因表現之分析 | zh_TW |
| dc.title | Expression Analyses for Fruit Ripening-Involved Genes in Transgenic banana RNAi lines of 1-Aminocyclopropane-1-Carboxylate Oxidase Genes | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 102-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.coadvisor | 黃鵬林(Pung-Ling Huang) | |
| dc.contributor.oralexamcommittee | 何國傑(Kuo-Chieh Ho),洪傳揚(Chwan-Yang Hong) | |
| dc.subject.keyword | ACC氧化?,RNA干擾,果實後熟, | zh_TW |
| dc.subject.keyword | ACC oxidase,RNA interference,fruit ripening, | en |
| dc.relation.page | 57 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2014-08-12 | |
| dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
| dc.contributor.author-dept | 園藝學研究所 | zh_TW |
| Appears in Collections: | 園藝暨景觀學系 | |
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