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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 于宏燦(Hon-Tsen Yu) | |
dc.contributor.author | Po-Yu Liu | en |
dc.contributor.author | 劉勃佑 | zh_TW |
dc.date.accessioned | 2021-06-16T05:39:34Z | - |
dc.date.available | 2015-08-21 | |
dc.date.copyright | 2014-08-21 | |
dc.date.issued | 2014 | |
dc.date.submitted | 2014-08-12 | |
dc.identifier.citation | 呂曉沛,2013。以多源基因體與多源轉錄體方法研究食葉性白面鼯鼠 (Petaurista alborufus lena)的消化道微生物菌群結構與功能。國立臺灣大學動物學研究所博士論文。
李培芬,1983。大赤鼯鼠之生殖與生態研究。國立臺灣大學動物學研究所碩士論文。 李培芬,1998a。台灣地區飛鼠研究之回顧。台灣日本飛鼠松鼠保育與經營管理研討會。國立臺灣大學動物系, 東海大學生物學系。 李培芬,1998b。臺灣的飛鼠。農委會林務局。 郭奇芊,1999。福山試驗林大赤鼯鼠之食性、活動範圍及活動模式。國立臺灣大學動物學研究所碩士論文。 Aw, T. Y. 2003. Cellular Redox: A Modulator of Intestinal Epithelial Cell Proliferation. Physiology, 18(5), 201-204. Baron, Stephen F., & Hylemon, Phillip B. 1997. Biotransformation of Bile Acids, Cholesterol, and Steroid Hormones. Gastrointestinal Microbiology (R. Mackie & B. White, Eds.). pp. 470-510. Springer US. Cheung, Eric C., Athineos, D., Lee, P., Ridgway, Rachel A., Lambie, W., Nixon, C., Vousden, Karen H. 2013. TIGAR Is Required for Efficient Intestinal Regeneration and Tumorigenesis. Developmental Cell, 25(5), 463-477. Dryden, G. M. 2008a. Digestion and the Supply of Nutrients. Animal Nutrition Science. pp. 85-114. CABI. Dryden, G. M. 2008b. Feed Mill Design and Management: an Introduction Animal Nutrition Science. pp. 273-291. CABI. Dryden, G. M. 2008c. Rational Formulation. Animal Nutrition Science. pp. 218-225. CABI. Eisenberg, J. F. 1978. The evolution of arboreal herbivores in the class Mammalia. The ecology of arboreal folivores (GG Montgomery, ed.). Smithsonian Institution Press, Washington, DC, 574, 135-152. Erban, A., Schauer, N., Fernie, A. R., & Kopka, J. 2006. Nonsupervised Construction and Application of Mass Spectral and Retention Time Index Libraries From Time-of-Flight Gas Chromatography-Mass Spectrometry Metabolite Profiles. Metabolomics, Vol. 358, pp. 19-38. Springer. Fell, D. A. 2010. Evolution of Central Carbon Metabolism. Molecular Cell, 39(5), 663-664. Frickenschmidt, A., Frohlich, H., Bullinger, D., Zell, A., Laufer, S., Gleiter, C. H., Kammerer, B. 2008. Metabonomics in cancer diagnosis: mass spectrometry-based profiling of urinary nucleosides from breast cancer patients. Biomarkers, 13(4), 435-449. Hume, I. D. 2005. Concepts of Digestive Efficiency. In J. M. Starck & T. Wang (Eds.), Physiological and ecological adaptations to feeding in vertebrates. pp. 43-58. Enfield, NH: Science Publishers. Kanehisa, M., & Goto, S. 2000. KEGG: kyoto encyclopedia of genes and genomes. Nucleic Acids Res, 28(1), 27-30. Kanehisa, M., Goto, S., Sato, Y., Kawashima, M., Furumichi, M., & Tanabe, M. 2014. Data, information, knowledge and principle: back to metabolism in KEGG. Nucleic Acids Res, 42(Database issue), D199-205. Kuo, C.-C., & Lee, L.-L. 2003. Food Availability and Food Habits of Indian Giant Flying Squirrels (Petaurista philippensis) in Taiwan. Journal of Mammalogy, 84(4), 1330-1340. Kuo, C.-C., & Lee, L.-L. 2012. Home range and activity of the Indian giant flying squirrel (Petaurista philippensis) in Taiwan: influence of diet, temperature, and rainfall. Acta Theriologica, 57(3), 269-276. Lu, H.-P., Wang, Y.-b., Huang, S.-W., Lin, C.-Y., Wu, M., Hsieh, C.-h., & Yu, H.-T. 2012. Metagenomic analysis reveals a functional signature for biomass degradation by cecal microbiota in the leaf-eating flying squirrel (Petaurista alborufus lena). BMC Genomics, 13(1), 466. Matsumoto, M., Kibe, R., Ooga, T., Aiba, Y., Kurihara, S., Sawaki, E., Benno, Y. 2012. Impact of Intestinal Microbiota on Intestinal Luminal Metabolome. Sci. Rep., 2. Matsumoto, M., Kibe, R., Ooga, T., Aiba, Y., Sawaki, E., Koga, Y., & Benno, Y. 2013. Cerebral low-molecular metabolites influenced by intestinal microbiota: a pilot study. Frontiers in Systems Neuroscience, 7. Morgan, X., Tickle, T., Sokol, H., Gevers, D., Devaney, K., Ward, D., Huttenhower, C. 2012. Dysfunction of the intestinal microbiome in inflammatory bowel disease and treatment. Genome Biology, 13(9), R79. Morgenthal, K., Wienkoop, S., Wolschin, F., & Weckwerth, W. 2006. Integrative Profiling of Metabolites and Proteins Metabolomics, Vol. 358, pp. 57-75. Springer. Nakayama, H., & Weser, E. 1972. Adaptation of small bowel after intestinal resection: increase in the pentose phosphate pathway. Biochim Biophys Acta, 279(3), 416-423. Noor, E., Eden, E., Milo, R., & Alon, U. 2010. Central Carbon Metabolism as a Minimal Biochemical Walk between Precursors for Biomass and Energy. Molecular Cell, 39(5), 809-820. Ohdoi, C., Nyhan, W. L., & Kuhara, T. 2003. Chemical diagnosis of Lesch–Nyhan syndrome using gas chromatography-mass spectrometry detection. Journal of Chromatography B, 792(1), 123-130. Oliver, S. G., Winson, M. K., Kell, D. B., & Baganz, F. 1998. Systematic functional analysis of the yeast genome. Trends in Biotechnology, 16(9), 373-378. Ott, K.-H., & Aranibar, N. 2006. Nuclear Magnetic Resonance Metabonomics Metabolomics, Vol. 358, pp. 247-271. Springer. Pauling, L., Robinson, A. B., Teranishi, R., & Cary, P. 1971. Quantitative Analysis of Urine Vapor and Breath by Gas-Liquid Partition Chromatography. Proceedings of the National Academy of Sciences, 68(10), 2374-2376. Rothman, N., World Health Organization, & International Agency for Research on Cancer. 2011. Molecular epidemiology: Principles and practices. Lyon, France: International Agency for Research on Cancer, World Health Organization, 132. Scholz, M., & Selbig, J. 2006. Visualization and Analysis of Molecular Data Metabolomics, Vol. 358, pp. 87-104. Springer. Shimodaira, H. 2004. Approximately Unbiased Tests of Regions Using Multistep-Multiscale Bootstrap Resampling. The Annals of Statistics, 32(6), 2616-2641. Soga, T. 2006. Capillary Electrophoresis-Mass Spectrometry for Metabolomics Metabolomics, Vol. 358, pp. 129-137. Springer. Steuer, R., Morgenthal, K., Weckwerth, W., & Selbig, J. 2006. A Gentle Guide to the Analysis of Metabolomic Data Metabolomics, Vol. 358, pp. 105-126. Stevens, C. E., & Hume, I. D. 2004a. Digestion of carbohydrate, lipids, and protein and the absorption of end products Comparative Physiology of the Vertebrate Digestive System. pp. 152-187. Cambridge University Press. Stevens, C. E., & Hume, I. D. 2004b. Microbial fermentation and synthesis of nutrients and the absorption of end products Comparative Physiology of the Vertebrate Digestive System. pp. 188-228. Cambridge University Press. Suzuki, R., & Shimodaira, H. 2006. Pvclust: an R package for assessing the uncertainty in hierarchical clustering. Bioinformatics, 22(12), 1540-1542. Tolstikov, V. V., Fiehn, O., & Tanaka, N. 2006. Application of Liquid Chromatography-Mass Spectrometry Analysis in Metabolomics Metabolomics, Vol. 358, pp. 141-155. Urich, K. 1994. Comparative animal biochemistry: Springer-Verlag. Viant, M. R. 2006. Revealing the Metabolome of Animal Tissues Using 1H Nuclear Magnetic Resonance Spectroscopy Metabolomics, Vol. 358, pp. 229-246. Springer. Weigoldt, M., Meens, J., Bange, F. C., Pich, A., Gerlach, G. F., & Goethe, R. 2013. Metabolic adaptation of Mycobacterium avium subsp. paratuberculosis to the gut environment. Microbiology, 159(Pt 2), 380-391. Wishart, D. S., Jewison, T., Guo, A. C., Wilson, M., Knox, C., Liu, Y. F., Scalbert, A. 2013. HMDB 3.0-The Human Metabolome Database in 2013. Nucleic Acids Research, 41(D1), D801-D807. Wishart, D. S., Knox, C., Guo, A. C., Eisner, R., Young, N., Gautam, B., Forsythe, I. 2009. HMDB: a knowledgebase for the human metabolome. Nucleic Acids Research, 37, D603-D610. Wishart, D. S., Tzur, D., Knox, C., Eisner, R., Guo, A. C., Young, N., Querengesser, L. 2007. HMDB: the human metabolome database. Nucleic Acids Research, 35, D521-D526. Xia, J. G., Mandal, R., Sinelnikov, I. V., Broadhurst, D., & Wishart, D. S. 2012. MetaboAnalyst 2.0-a comprehensive server for metabolomic data analysis. Nucleic Acids Research, 40(W1), W127-W133. Xia, J. G., Psychogios, N., Young, N., & Wishart, D. S. 2009. MetaboAnalyst: a web server for metabolomic data analysis and interpretation. Nucleic Acids Research, 37, W652-W660. Yuan, M., Breitkopf, S. B., Yang, X., & Asara, J. M. 2012. A positive/negative ion-switching, targeted mass spectrometry-based metabolomics platform for bodily fluids, cells, and fresh and fixed tissue. Nat Protoc, 7(5), 872-881. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/56643 | - |
dc.description.abstract | 食葉性哺乳動物為一有效利用能量及與消化道微生物共生適應的植食性哺乳動物,其以富含纖維素的成熟葉子為食。大赤鼯鼠(Petaurista philippensis grandis)和白面鼯鼠(P. alborufus lena)是台灣典型的食葉性哺乳動物,膨大及延長的盲腸是與其他後腸發酵動物共通的消化道結構,且為提供共生微生物進行厭氧代謝之處。代謝產物為代謝過程中的產物,即反映動物的生理和代謝狀態及消化道的化學環境。本研究透過液相層析質譜儀非標的性之代謝體分析大鼠和飛鼠消化道內容物的代謝產物,比較馴養之實驗大鼠,在餵食一般飼料或高纖維飼料者,與食葉性飛鼠的消化道代謝產物組成差異和代謝途徑之生化反應動態的代謝體學。主成份分析配合K-means分群法發現大鼠和飛鼠的消化道內容物代謝產物組成與消化道的解剖結構、位置分佈相關,且消化的時間會減少代謝產物組成的變異程度。而五碳糖磷酸途徑可能為飛鼠後腸高度表現的代謝途徑,推測與提供脂肪酸等植物性食物來源缺乏之養分的生合成有關。 | zh_TW |
dc.description.abstract | Folivorous mammals, which consume mature leaves, are able to extract energy from leave cellulose, with the aid from microbes inhabiting in their guts. Two flying squirrels in Taiwan, red-giant flying squirrel (Petaurista philippensis grandis) and white-faced flying squirrel (P. alborufus lena) that contain enlarged caeca (where microbial fermentation happens) are typical folivorous mammals. Metabolites are the products of metabolism and reflect physiological status and gastrointestinal chemical environment of an animal. We analyzed untargeted gut metabolic substance of the flying squirrels by liquid chromatography-mass spectrometry (LC-MS). Specifically, we compare flying squirrel with laboratory rats to reveal the difference between wild folivores (flying squirrel) and domesticated laboratory rats, especially to highlight the uniqueness of hindgut metabolome of flying squirrels. We found that metabolite composition corresponds to gut anatomical structures with metabolite groups revealed by principle component analysis (PCA) coupled with K-means cluster analysis. Variation of metabolite composition also relates to digestive time. In addition, Pentose phosphate pathway (PPP) might be a highly expressed pathway in flying squirrels’ hindguts. PPP in flying squirrel hindgut might relate to biosynthesis of certain essential nutrients (e.g. fatty acids which lack in leaves). | en |
dc.description.provenance | Made available in DSpace on 2021-06-16T05:39:34Z (GMT). No. of bitstreams: 1 ntu-103-R01b41015-1.pdf: 2951463 bytes, checksum: 2ac1d1341d04a655541dde3c7f47610f (MD5) Previous issue date: 2014 | en |
dc.description.tableofcontents | 口試委員會審定書 #
致謝 i 中文摘要 ii ABSTRACT iii 目錄 iv 圖目錄 vii 表目錄 viii 第1章 前言 1 1.1 植食性哺乳動物的食性與消化 1 1.2 腸道共生微生物—二次代謝與發酵 1 1.3 研究對象描述 2 1.4 代謝體學簡介 3 1.4.1 比較生物化學 3 1.4.2 代謝體與代謝體學 3 1.4.3 代謝體學研究技術發展 4 1.4.4 代謝體學研究方法 5 1.5 研究目的 6 第2章 材料與方法 7 2.1 實驗方法之建立–大鼠系統 7 2.2 食葉性飛鼠採集 7 2.3 消化道內容物採樣 7 2.4 液相層析–電噴灑–質譜術(LC-ESI-MS)分析消化道內容物代謝體 8 2.4.1 樣本前處理與樣本製備 8 2.4.2 分析儀器系統與參數設定 8 2.5 建立代謝體資料庫 9 2.5.1 宿主代謝體參考資料庫 9 2.5.2 共生微生物代謝體參考資料庫 10 2.6 原始數據處理 10 2.7 資料探勘與統計分析 11 2.7.1 資料探勘之減維分析 11 2.7.2 代謝途徑分析 11 第3章 結果 12 3.1 比較食葉性飛鼠與馴養之實驗大鼠的消化道代謝體組成 12 3.2 飛鼠後腸之高影響力代謝產物與代謝途徑 13 第4章 討論 14 4.1 食性、消化與代謝之關係探討 14 4.1.1 消化順序與代謝產物組成的變化 14 4.2 食葉性飛鼠後腸之五碳糖磷酸途徑 15 4.2.1 消化道中的五碳糖磷酸途徑 16 參考文獻 17 圖 21 表 28 附錄 29 附錄一 階層集群分析驗證K-means法對消化道代謝體操作單元的分群 29 附錄二 以飼料及胃部代謝體資訊定義食物代謝體組成 31 附錄三 其他圖表 34 | |
dc.language.iso | zh-TW | |
dc.title | 植食性哺乳動物消化道之代謝體學研究 | zh_TW |
dc.title | Gastrointestinal Metabolomic Study of Herbivorous Mammals | en |
dc.type | Thesis | |
dc.date.schoolyear | 102-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 廖本揚(Ben-Yang Liao),郭志鴻(Chih-Horng Kuo),王涵青(Han-Ching Wang),黃曉薇(Shiao-Wei Huang) | |
dc.subject.keyword | 食葉性哺乳動物,大赤鼯鼠,白面鼯鼠,代謝體學,五碳糖磷酸途徑, | zh_TW |
dc.subject.keyword | folivrous mammals,red-giant flying squirrel,Petaurista philippensis grandis,white-faced flying squirrel,P. alborufus lena,metabolomics,pentose phosphate pathway, | en |
dc.relation.page | 36 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2014-08-12 | |
dc.contributor.author-college | 生命科學院 | zh_TW |
dc.contributor.author-dept | 生命科學系 | zh_TW |
顯示於系所單位: | 生命科學系 |
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