請用此 Handle URI 來引用此文件：
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/56554完整後設資料紀錄
| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 吳先琪(Shian-chee Wu) | |
| dc.contributor.author | Yi-An Tsai | en |
| dc.contributor.author | 蔡宜安 | zh_TW |
| dc.date.accessioned | 2021-06-16T05:34:34Z | - |
| dc.date.available | 2019-08-17 | |
| dc.date.copyright | 2014-08-17 | |
| dc.date.issued | 2014 | |
| dc.date.submitted | 2014-08-13 | |
| dc.identifier.citation | Auffan, M., Achouak, W., Rose, J., Roncato, M. A., Chaneac, C., Waite, D. T., Masion, A., Woicik, J. C., Wiesner, M. R., and Bottero, J. Y. (2008). Relation between the redox state of iron-based nanoparticles and their cytotoxicity toward Escherichia coli. Environ. Sci. Technol., 42(17), 6730-6735.
Bader, H., Sturzenegger, V., and Hoigne, J. (1988). Photometric method for the determination of low concentrations of hydrogen peroxide by the peroxidase catalyzed oxidation of N,N-diethyl-p-phenylenediamine (DPD). Water Research, 22(9), 1109-1115. Brenner, S. (1974). Genetics of Caenorhabditis-Elegans. Genetics, 77(1), 71-94. Byerly, L., Cassada, R. C., and Russell, R. L. (1976). The life cycle of the nematode Caenorhabditis elegans: I. Wild-type growth and reproduction. Developmental Biology, 51(1), 23-33. Che, C. H. (2008). Preparation of nanoscale zero-valent iron suspension and its transport model in aaturated aoil layer. Master Thesis. Graduate Institute of Envuronmental Engineering of National Taiwan University. Chen, J., and Caswell-Chen, E. P. (2004). Facultative vivipary is a life-history trait in Caenorhabditis elegans. Journal of nematology, 36(2), 107. Chen, J., Xiu, Z., Lowry, G. V., and Alvarez, P. J. J. (2011a). Effect of natural organic matter on toxicity and reactivity of nano-scale zero-valent iron. Water Research, 45(5), 1995-2001. Chen, P. H., Hsiao, K. M., and Chou, C. C. (2013). Molecular characterization of toxicity mechanism of single-walled carbon nanotubes. Biomaterials, 34(22), 5661-5669. Chen, P. J., Su, C. H., Tseng, C. Y., Tan, S. W., and Cheng, C. H. (2011b). Toxicity assessments of nanoscale zerovalent iron and its oxidation products in medaka (Oryzias latipes) fish. Marine Pollution Bulletin, 63(5–12), 339-346. Chen, P. J., Tan, S. W., and Wu, W. L. (2012). Stabilization or Oxidation of Nanoscale Zerovalent Iron at Environmentally Relevant Exposure Changes Bioavailability and Toxicity in Medaka Fish. Environ. Sci. Technol., 46(15), 8431-8439. de Best, J. H. (1999). Anaerobic transformation of chlorinated hydrocarbons in a packed-bed reactor. Ph. D. Thesis. University of Groningen. Gillham, R. W., and O'Hannesin, S. F. (1994). Enhanced degradation of halogenated aliphatics by zero-valent iron. Ground Water, 32(6), 958-967. Glavee, G. N., Klabunde, K. J., Sorensen, C. M., and Hadjipanayis, G. C. (1995). Chemistry of borohydride reduction of iron(II) and iron(III) ions in aqueous and nonaqueous media. formation of nanoscale Fe, FeB, and Fe2B powders. Inorganic Chemistry, 34(1), 28-35. Grieger, K. D., Fjordboge, A., Hartmann, N. B., Eriksson, E., Bjerg, P. L., and Baun, A. (2010). Environmental benefits and risks of zero-valent iron nanoparticles (nZVI) for in situ remediation: Risk mitigation or trade-off? J. Contam. Hydrol., 118(3-4), 165-183. He, D. H. (1994). Analysis of dissolves ferrous & ferric iron and their applications to natural waters. Master Thesis. Department of Oceanography of National Taiwan University. He, F., and Zhao, D. (2005). Preparation and characterization of a new class of starch-stabilized bimetallic nanoparticles for degradation of chlorinated hydrocarbons in water. Environ. Sci. Technol., 39(9), 3314-3320. He, F., and Zhao, D. Y. (2007). Manipulating the size and dispersibility of zerovalent iron nanoparticles by use of carboxymethyl cellulose stabilizers. Environ. Sci. Technol., 41(17), 6216-6221. Hunt, P. R., Marquis, B. J., Tyner, K. M., Conklin, S., Olejnik, N., Nelson, B. C., and Sprando, R. L. (2013). Nanosilver suppresses growth and induces oxidative damage to DNA in Caenorhabditis elegans. J. Appl. Toxicol., 33(10), 1131-1142. Hyeon, T., Lee, S. S., Park, J., Chung, Y., and Na, H. B. (2001). Synthesis of highly crystalline and monodisperse maghemite nanocrystallites without a size-selection process. Journal of the American Chemical Society, 123(51), 12798-12801. Kang, Y. S., Risbud, S., Rabolt, J. F., and Stroeve, P. (1996). Synthesis and characterization of nanometer-size Fe3O4 and γ-Fe2O3 Particles. Chemistry of Materials, 8(9), 2209-2211. Katsoyiannis, I. A., Ruettimann, T., and Hug, S. J. (2008). pH dependence of fenton reagent generation and As(III) oxidation and removal by corrosion of zero valent iron in aerated water. Environ. Sci. Technol., 42(19), 7424-7430. Keenan, C. R., Goth-Goldstein, R., Lucas, D., and Sedlak, D. L. (2009). Oxidative stress induced by zero-valent iron nanoparticles and Fe(II) in human bronchial epithelial cells. Environ. Sci. Technol., 43(12), 4555-4560. Khanna, N., Cressman, C. P., Tatara, C. P., and Williams, P. L. (1997). Tolerance of the nematode Caenorhabditis elegans to pH, salinity, and hardness in aquatic media. Arch. Environ. Contam. Toxicol., 32(1), 110-114. Kim, J., Takahashi, M., Shimizu, T., Shirasawa, T., Kajita, M., Kanayama, A., and Miyamoto, Y. (2008). Effects of a potent antioxidant, platinum nanoparticle, on the lifespan of Caenorhabditis elegans. Mech. Ageing. Dev., 129(6), 322-331. Klaine, S. J., Alvarez, P. J. J., Batley, G. E., Fernandes, T. F., Handy, R. D., Lyon, D. Y., Mahendra, S., McLaughlin, M. J., and Lead, J. R. (2008). Nanomaterials in the environment: Behavior, fate, bioavailability, and effects. Environ. Toxicol. Chem., 27(9), 1825-1851. Konstadinidis, K., Thakkar, B., Chakraborty, A., Potts, L. W., Tannenbaum, R., Tirrell, M., and Evans, J. F. (1992). Segment level chemistry and chain conformation in the reactive adsorption of poly(methyl methacrylate) on aluminum oxide surfaces. Langmuir, 8(5), 1307-1317. Lee, C., Kim, J. Y., Lee, W. I., Nelson, K. L., Yoon, J., and Sedlak, D. L. (2008). Bactericidal effect of zero-valent iron nanoparticles on Escherichia coli. Environ. Sci. Technol., 42(13), 4927-4933. Leung, M. C. K., Williams, P. L., Benedetto, A., Au, C., Helmcke, K. J., Aschner, M., and Meyer, J. N. (2008). Caenorhabditis elegans: An emerging model in biomedical and environmental toxicology. Toxicological Sciences, 106(1), 5-28. Li, Greden, K., Alvarez, P. J. J., Gregory, K. B., and Lowry, G. V. (2010). Adsorbed polymer and NOM limits adhesion and toxicity of nano scale zerovalent iron to E. coli. Environ. Sci. Technol., 44(9), 3462-3467. Li, Zhou, Q., Wu, Y., Fu, J., Wang, T., and Jiang, G. (2009). Effects of waterborne nano-iron on medaka (Oryzias latipes): Antioxidant enzymatic activity, lipid peroxidation and histopathology. Ecotoxicology and Environmental Safety, 72(3), 684-692. Li, Y., Yu, S., Wu, Q., Tang, M., Pu, Y., and Wang, D. (2012). Chronic Al2O3-nanoparticle exposure causes neurotoxic effects on locomotion behaviors by inducing severe ROS production and disruption of ROS defense mechanisms in nematode Caenorhabditis elegans. J. Hazard. Mater., 219–220(0), 221-230. Lin, H. W. (2011). Synthesis of dense non aqueous suspension of nano-scale zero valent iron. Master Thesis. Graduate Institute of Envuronmental Engineering of National Taiwan University. Liu, P. G. (2010). Preparation of dense non-aqueous phase nano zero-valent iron suspension for environmental restoration. Master Thesis. Graduate Institute of Environmental Engineering of National Taiwan University. Liufu, S., Xiao, H., and Li, Y. (2005). Adsorption of poly(acrylic acid) onto the surface of titanium dioxide and the colloidal stability of aqueous suspension. Journal of Colloid and Interface Science, 281(1), 155-163. Lowry, G. V., Gregory, K. B., Apte, S. C., and Lead, J. R. (2012). Transformations of nanomaterials in the environment. Environ. Sci. Technol., 46(13), 6893-6899. Ma, H., Bertsch, P. M., Glenn, T. C., Kabengi, N. J., and Williams, P. L. (2009). Toxicity of manufactured zinc oxide nanoparticles in the nematode Caenorhabditis elegans. Environ. Toxicol. Chem., 28(6), 1324-1330. doi: 10.1897/08-262.1 Matheson, L. J., and Tratnyek, P. G. (1994). Reductive dehalogenation of chlorinated methanes by iron metal. Environ. Sci. Technol., 28(12), 2045-2053. Meyer, J. N., Lord, C. A., Yang, X. Y., Turner, E. A., Badireddy, A. R., Marinakos, S. M., Chilkoti, A., Wiesner, M. R., and Auffan, M. (2010). Intracellular uptake and associated toxicity of silver nanoparticles in Caenorhabditis elegans. Aquatic Toxicology, 100(2), 140-150. Nel, A., Xia, T., Madler, L., and Li, N. (2006). Toxic potential of materials at the nanolevel. Science, 311(5761), 622-627. Phenrat, T., Long, T. C., Lowry, G. V., and Veronesi, B. (2008). Partial oxidation (“aging”) and surface modification decrease the toxicity of nanosized zerovalent iron. Environ. Sci. Technol., 43(1), 195-200. Phenrat, T., Saleh, N., Sirk, K., Kim, H.-J., Tilton, R. D., and Lowry, G. V. (2007). Stabilization of aqueous nanoscale zerovalent iron dispersions by anionic polyelectrolytes: adsorbed anionic polyelectrolyte layer properties and their effect on aggregation and sedimentation. Journal of Nanoparticle Research, 10(5), 795-814. Pluskota, A., Horzowski, E., Bossinger, O., and von Mikecz, A. (2009). In Caenorhabditis elegans nanoparticle-bio-interactions become transparent: silica-nanoparticles induce reproductive senescence. PLoS One, 4(8), 9. Saleh, N., Kim, H.-J., Phenrat, T., Matyjaszewski, K., Tilton, R. D., and Lowry, G. V. (2008). Ionic strength and composition affect the mobility of surface-modified Fe0 nanoparticles in water-saturated sand columns. Environ. Sci. Technol., 42(9), 3349-3355. Saleh, N., Phenrat, T., Sirk, K., Dufour, B., Ok, J., Sarbu, T., Matyjaszewski, K., Tilton, R. D., and Lowry, G. V. (2005). Adsorbed triblock copolymers deliver reactive iron nanoparticles to the oil/water interface. Nano Letters, 5(12), 2489-2494. Salkar, R. A., Jeevanandam, P., Kataby, G., Aruna, S. T., Koltypin, Y., Palchik, O., and Gedanken, A. (2000). Elongated copper nanoparticles coated with a zwitterionic surfactant. The Journal of Physical Chemistry B, 104(5), 893-897. Schafer, W. R. (2006). Genetics of egg-laying in worms. Annual Review of Genetics, 40(1), 487-509. Schrick, B., Blough, J. L., Jones, A. D., and Mallouk, T. E. (2002). Hydrodechlorination of trichloroethylene to hydrocarbons using bimetallic nickel−iron nanoparticles. Chemistry of Materials, 14(12), 5140-5147. Schrick, B., Hydutsky, B. W., Blough, J. L., and Mallouk, T. E. (2004). Delivery vehicles for zerovalent metal nanoparticles in soil and groundwater. Chemistry of Materials, 16(11), 2187-2193. Scott-Fordsmand, J. J., Krogh, P. H., Schaefer, M., and Johansen, A. (2008). The toxicity testing of double-walled nanotubes-contaminated food to Eisenia veneta earthworms. Ecotoxicology and Environmental Safety, 71(3), 616-619. Stohs, S. J., and Bagchi, D. (1995). Oxidative mechanisms in the toxicity of metal ions. Free Radical Biology and Medicine, 18(2), 321-336. Stookey, L. L. (1970). Ferrozine---a new spectrophotometric reagent for iron. Analytical Chemistry, 42(7), 779-781. Sun, Y.-P., Li, X.-Q., Zhang, W.-X., and Wang, H. P. (2007). A method for the preparation of stable dispersion of zero-valent iron nanoparticles. Colloids and Surfaces A: Physicochemical and Engineering Aspects, 308(1–3), 60-66. Tang, E., Cheng, G., Pang, X., Ma, X., and Xing, F. (2006). Synthesis of nano-ZnO/poly(methyl methacrylate) composite microsphere through emulsion polymerization and its UV-shielding property. Colloid Polym Sci, 284(4), 422-428. Voelker, B. M., and Sulzberger, B. (1996). Effects of fulvic acid on Fe(II) oxidation by hydrogen peroxide. Environ. Sci. Technol., 30(4), 1106-1114. Wang, H. H., Wick, R. L., and Xing, B. S. (2009). Toxicity of nanoparticulate and bulk ZnO, Al2O3 and TiO2 to the nematode Caenorhabditis elegans. Environ. Pollut., 157(4), 1171-1177. Wang, W., Gu, B., Liang, L., and Hamilton, W. A. (2004). Adsorption and structural arrangement of cetyltrimethylammonium cations at the silica nanoparticle−water interface. The Journal of Physical Chemistry B, 108(45), 17477-17483. Wang, W., Zhou, M., Jin, Z., and Li, T. (2010). Reactivity characteristics of poly(methyl methacrylate) coated nanoscale iron particles for trichloroethylene remediation. J. Hazard. Mater., 173(1–3), 724-730. Yan, L., Gu, Z., and Zhao, Y. (2013). Chemical mechanisms of the toxicological properties of nanomaterials: Generation of intracellular reactive oxygen species. Chemistry – An Asian Journal, 8(10), 2342-2353. Zhang, W. X., Wang, C. B., and Lien, H. L. (1998). Treatment of chlorinated organic contaminants with nanoscale bimetallic particles. Catalysis Today, 40(4), 387-395. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/56554 | - |
| dc.description.abstract | 奈米零價鐵 (Nanoscale zerovalent iron, NZVI) 具有粒徑小、比表面積大及強還原性等特性，被廣泛運用於現地土壤與地下水的污染整治中。研究指出經表面修飾過的NZVI可以提升在地下水中的移動速率，若以疏水性表面修飾劑修飾更可以提升油相污染物的移除速率。整治過程中大量的NZVI釋放於環境中，但對於NZVI在地下水中的宿命、生態環境及人體健康的衝擊尚未明朗。本研究以秀麗隱桿線蟲 (Caenorhabditiselegans, C. elegans) 為模式動物，探討未修飾NZVI，或以聚丙烯酸 (PAA)、十六烷基三甲基溴化銨 (CTAB) 及聚甲基丙烯酸甲酯 (PMMA) 修飾之NZVI在不同溶液中，以及亞鐵離子及奈米氧化鐵 (Fe2O3) 之毒性。藉由觀察線蟲的死亡率、繁殖率及體長改變以了解NZVI是否會對生物造成影響。在24小時急性暴露過程中，修飾及未修飾NZVI、Fe2+及Fe2O3對線蟲的死亡率影響不顯著。經48小時暴露後，線蟲的體長受到NZVI的影響，而Fe2+的影響更為明顯。經72小時的暴露，線蟲的繁殖率明顯受到NZVI及Fe2+影響，在高濃度暴露下 (500、750 mg/L) 線蟲無法產出後代。同時在高濃度暴露下 (500、750 mg/L) 我們觀察到體內孵化 (bag of worms, BOW) 的情形發生，可能是因為NZVI產生的Fe2+及活性氧物種(reactive oxygen species, ROS) 對線蟲造成氧化壓力使得線蟲無法產卵而使幼蟲在體內孵化。Fe2O3對體長及繁殖率沒有造成影響。此外在顯微鏡的觀察下，NZVI會附著在線蟲的外表上，而線蟲也會攝食NZVI，與體內氧氣反應產生ROS對線蟲造成毒性。雖然在短期暴露之下NZVI的毒性不顯著，但若以長期來看仍會對生物造成影響。 | zh_TW |
| dc.description.abstract | Nanoscale zero-valent iron (NZVI) has been used for the remediation of contaminated groundwater as a reductant. In order to enhance its mobility, NZVI has been surface-modified with polymers or surfactants to increase its stability in aqueous phase. Hydrophobic modifiers will also improve the affinity of NZVI with non-aqueous phase liquids. Although great amount of NZVI may be to release to the environment, the fate and ecotoxicological effects of NZVI remain unclear. In this study we investigated the toxicity of bare NZVI, polyacrylic acid (PAA), polymethylmethacrylate (PMMA) or cetyltrimethylammonium bromide (CTAB) modified NZVIs in different solution phases, Fe2O3 nanoparticles and Fe2+ on nematodes, Caenorhabditis elegans (C. elegans), by using different toxic endpoint such as lethality, reproductivity and morphological change. Under acute exposure (24 h), C. elegans were slightly affected by various types of NZVI suspensions, Fe2O3 and Fe2+. After 48h of exposure, the lengths of C. elegans were affected by all types of NZVI suspensions, as well as Fe2+ solution. After 72h of expose, the reproductivity of C. elegans was significantly decreased by NZVI suspensions and Fe2+ solution. Fe2O3 showed no toxicity to C. elegans. We have also observed the ‘bag of worms’ phenotype, which is characterized by failed egg-laying and may occur in the stressed environment which is produced by the oxidation of NZVI and the release of Fe2+ and reactive oxygen species. From the observation under the microscope, we found that NZVI particles were attached on C. elegans and inside its intestinal track which would react with internal substances and produce ROS causing the toxicity. The experimental results showed that although the stable NZVIs may not cause death in C. elegans, but it does pose adverse effects other than death, which are the potential impact of NZVI. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-16T05:34:34Z (GMT). No. of bitstreams: 1 ntu-103-R01541116-1.pdf: 2814974 bytes, checksum: 5ea08a08088fafa054f81e7595efa657 (MD5) Previous issue date: 2014 | en |
| dc.description.tableofcontents | 摘要 I
Abstract III Contents V List of Figures VIII List of Tables XI Chapter 1 Introduction 1 1.1 General background 1 1.2 Research purpose 4 Chapter 2 Literature Review 5 2.1 Interaction between NZVI and pollutants 5 2.1.1 Redox theory of NZVI 5 2.1.2 Reaction pathway of NZVI 6 2.1.2 Synthesis of bare NZVI 8 2.1.3 Modification of NZVI 9 2.1.4 Dense non-aqueous phase NZVI 11 2.2 Fate and behavior of NZVI in the environment 12 2.3 The toxic effect of NZVI to organisms 14 2.3.1 Effects of ROS generation 15 2.3.2 Damage to cell structure 16 2.4 Ecotoxicity of NZVI 17 2.5 Model animal ─Caenorhabditis elegans 21 2.5.1 Caenorhabditis elegans 21 2.5.2 Life cycle of C. elegans 22 2.5.3 Advantages of C. elegans on research 24 2.5.4 Assessing parameters 24 Chapter 3 Materials and Methods 27 3.1 Experimental framework 27 3.2 Preparation of NZVI suspensions 29 3.2.1 Synthesis of unmodified NZVI 29 3.2.2 Modification of NZVI 29 3.3 Characterization of NZVI 31 3.3.1 Morphology of NZVI 31 3.3.2 Binding mechanism of surface modifiers 31 3.3.3 Suspendability in TCE 32 3.3.4 Contact angle 32 3.4 Dynamic change of NZVI exposure solution 32 3.4.1 pH and ORP 32 3.4.2 Iron species 33 3.4.3 Concentration of H2O2 34 3.5 Ecotoxicological assay 35 3.5.1 Culturing conditions 35 3.5.2 Synchronized nematodes 35 3.6 Lethality 36 3.7 Length change of C. elegans 37 3.8 Reproductivity 38 3.9 ROS production 38 Chapter 4 Results and Discussions 41 4.1 Characteristics of NZVIs 41 4.1.1 Morphology of NZVIs 41 4.1.2 Binding mechanism of surface modifiers 43 4.1.3 Suspendability in TCE 48 4.1.4 Contact angle 49 4.2 Dynamic change of NZVI exposure solutions 51 4.2.1 pH and ORP 51 4.2.2 Iron species 56 4.2.3 Change of H2O2 concentration 61 4.3 Ecotoxicological assay 66 4.3.1 Lethality 67 4.3.2 Length change of C. elegans 71 4.3.3 Reproductivity 74 4.3.4 Summary of ecotoxicological assay 79 4.3.5 In vivo influence 81 4.3.6 ROS production 87 Chapter 5 Conclusions 89 5.1 Conclusions 89 5.2 Suggestions 91 References 93 Appendix 99 | |
| dc.language.iso | en | |
| dc.subject | 秀麗隱桿線蟲 | zh_TW |
| dc.subject | 奈米零價鐵 | zh_TW |
| dc.subject | 表面修飾 | zh_TW |
| dc.subject | 生物毒性 | zh_TW |
| dc.subject | surface modification | en |
| dc.subject | nanoscale zero-valent iron | en |
| dc.subject | ecotocixity | en |
| dc.subject | Caenorhabditis elegans | en |
| dc.title | 修飾及未修飾奈米零價鐵對秀麗隱桿線蟲之毒性研究 | zh_TW |
| dc.title | Toxicity assessments of modified and unmodified nanoscale zerovalent iron to Caenorhabditis elegans | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 102-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 廖秀娟(Hsiu-Chuan Liao),陳佩貞(Pei-Jen Chen) | |
| dc.subject.keyword | 奈米零價鐵,表面修飾,秀麗隱桿線蟲,生物毒性, | zh_TW |
| dc.subject.keyword | nanoscale zero-valent iron,surface modification,Caenorhabditis elegans,ecotocixity, | en |
| dc.relation.page | 100 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2014-08-13 | |
| dc.contributor.author-college | 工學院 | zh_TW |
| dc.contributor.author-dept | 環境工程學研究所 | zh_TW |
| 顯示於系所單位： | 環境工程學研究所 | |
文件中的檔案：
| 檔案 | 大小 | 格式 | |
|---|---|---|---|
| ntu-103-1.pdf 未授權公開取用 | 2.75 MB | Adobe PDF |
系統中的文件，除了特別指名其著作權條款之外，均受到著作權保護，並且保留所有的權利。