請用此 Handle URI 來引用此文件：
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/56460完整後設資料紀錄
| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 林頌然(Sung-Jan Lin) | |
| dc.contributor.author | Pei-Shan Wu | en |
| dc.contributor.author | 吳佩珊 | zh_TW |
| dc.date.accessioned | 2021-06-16T05:29:41Z | - |
| dc.date.available | 2019-09-05 | |
| dc.date.copyright | 2014-09-05 | |
| dc.date.issued | 2014 | |
| dc.date.submitted | 2014-08-14 | |
| dc.identifier.citation | Alsantali, A. and J. Shapiro (2009). 'Androgens and hair loss.' Curr Opin Endocrinol Diabetes Obes 16(3): 246-253.
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Pacher-Zavisin, A. Yoshimura, P. M. Murphy and L. Hennighausen (2008). 'SOCS3 negatively regulates the gp130-STAT3 pathway in mouse skin wound healing.' J Invest Dermatol 128(7): 1821-1829. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/56460 | - |
| dc.description.abstract | 禿髮是現代人很常見的困擾，目前雖有治療的藥物來改善禿髮的問題，但藥物常有副作用的伴隨，停藥後容易有復發的情形。
透過先前的研究發現傷口的癒合也有助於毛髮的生長，在小鼠的實驗上已經觀察到小傷口可以使毛囊縮短休止期，提前進入生長期，使毛囊再生，但確切的機制目前仍不清楚。 結合這兩種概念利用1550奈米點陣鉺玻璃雷射，有別於一般傳統雷射的大面積破壞使用點陣式能量輸出能量、分段治療的概念，利用1550奈米點陣鉺玻璃雷射不同的能量強度與每平方公分的雷射光束密度，希望可以找到透過控制性小傷口不要造成疤痕組織的情形下來建立誘發毛再生的模式，並探討毛囊再生的相關機制。 由實驗結果中發現在我們所建立的1550奈米點陣鉺玻璃雷射使毛囊提前進入生長期的模式中，毛囊幹細胞與次級毛胚在受到1550奈米點陣鉺玻璃雷射的刺激後有提前活化的現象，而毛囊型態變化順序與一般毛髮生長週期相同，只是在本模式使毛囊縮短了休止期提前進入生長期。經1550奈米點陣鉺玻璃雷射刺激後有引起發炎相關基因TNF-α、IL-1β、IL-1R1、VEGF-a,d的表現有顯著增加，但顯著增高的表現只在刺激後的一到三天。而發炎細胞中嗜中性白血球也在第一天有顯著的表現；巨噬細胞則在雷射刺激前後則無明顯的差異。 我們進一步探討將1550奈米點陣餌玻璃雷射刺激所引起表現量顯著差異的發炎相關的基因使用抑制劑或是轉殖基因鼠將這些基因抑制掉來觀察缺少這些基因是否對本模式毛囊提前進入生長期的現象有所影響。結果發現VEGF receptor受抑制後本模式則無法提前進入生長期。由實驗可知VEGF在本模式中扮演了很重要的關鍵。 | zh_TW |
| dc.description.abstract | Alopecia is a common disease. Although hair drug therapy can improve alopecia, there is relapse after discontinuation of drug. Previous studies have found that wounding was able to induce hair regrowth and in vitro study mentioned that specific small wound, different from large wound induced by shortening telogen, but the mechanism is still not clarified. In this work, we use the 1,550-nm fractional erbium glass laser dot-matrix energy output and segmented treatment, which is different from the traditional widespread destruction laser, to establish a small wound-induced hair regeneration model, without scar tissue formation. Results show that 1,550-nm fractional erbium glass laser can induce premature anagen entry by activating hair follicle stem cells and secondary hair germ. After the 1,550-nm fractional erbium glass laser stimulation, expression of inflammation-related genes including TNF-α, IL-1β, IL-1R1, VEGF-a, d significantly increased from 24hr to 72hr. Besides, neutrophil infiltration is observed at 24h. We further found that inhibiting VEGF receptor phosphorylation could block laser induced hair regeneration. Therefore, VEGF signaling plays an important role in micro-injury induced follicle regeneration. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-16T05:29:41Z (GMT). No. of bitstreams: 1 ntu-103-R01548059-1.pdf: 9378862 bytes, checksum: b4e0645d11997a112b2529f07d8cdadc (MD5) Previous issue date: 2014 | en |
| dc.description.tableofcontents | 目錄
口試委員會審定書………………………………………………………………… Ⅰ 致謝 …………………………………………………………………………………Ⅱ 摘要 …………………………………………………………………………………Ⅳ Abstract ……………………………………………………………………………...Ⅴ 目錄 …………………………………………………………………………………Ⅵ 圖目錄 ………………………………………………………………………………Ⅷ 表目錄……………………………………………………………………………… Ⅹ 第一章 序論…………………………………………………………………………1 1.1 禿髮與治療…………………………………………………………………1 1.2 雷射療法與治療落髮………………………………………………………3 1.3 1,550奈米點陣鉺玻璃雷射 ………………………………………………5 1.4 傷口癒合……………………………………………………………………5 1.5 傷口癒合誘發毛囊新生……………………………………………………7 1.6 毛囊的構造…………………………………………………………………8 1.7 毛囊的週期 ……………………………………………………………… 10 第二章 實驗目的與設計……………………………………………………………11 2.1 實驗目的 …………………………………………………………………11 2.2 實驗設計 …………………………………………………………………12 第三章 材料與方法…………………………………………………………………14 3.1 動物施以飛梭雷射 ……………………………………………………… 14 3.2 Hematoxylin & Eosin染色………………………………………………16 3.3 免疫螢光染色 …………………………………………………………… 16 3.4 RNA萃取……………………………………………………………………19 3.5 逆轉錄聚合酶連鎖反應 ………………………………………………… 19 3.6 即時定量聚合酶連鎖反應 ……………………………………………… 20 第四章 實驗結果……………………………………………………………………23 4.1 建立控制性雷射誘發毛囊生長模式 …………………………………… 23 4.2 觀察控制性雷射誘發毛囊生長模式其細胞形態之改變 ……………… 37 4.3 探討控制性雷射對於皮膚以及毛囊的影響 …………………………… 39 4.4 探討控制性雷射對於活化毛囊幹細胞之分子機制 …………………… 41 4.5 探討控制性雷射誘發急性發炎誘發毛囊再生之機制 ………………… 45 4.6 免疫抑制劑及基因轉殖小鼠探討控制行雷射誘發毛囊生長之機制 … 56 4.7 探討控制性雷射誘發接觸性皮膚炎其細胞激素之角色 ……………… 61 第五章 討論…………………………………………………………………………67 第六章 結論…………………………………………………………………………70 第七章 參考資料……………………………………………………………………71 圖目錄 【圖1.6】毛囊的構造示意圖………………………………………………………9 【圖2.2】實驗設計流程……………………………………………………………13 【圖3.1.1】Fraxel re:store™(SR 1500)儀器面板 …………………………………14 【圖3.1.2】Fraxel re:store™(SR 1500)儀器、探頭與操作方法……………………15 【圖4.1.1】 5mj與其各密度對毛髮周期的影響…………………………………25 【圖4.1.2】10mj與其各密度對毛髮周期的影響…………………………………26 【圖4.1.3】12mj與其各密度對毛髮周期的影響…………………………………28 【圖4.1.4】15mj與其各密度對毛髮周期的影響…………………………………29 【圖4.1.5】20mj與其各密度對毛髮周期的影響…………………………………31 【圖4.1.6】25mj與其各密度對毛髮周期的影響…………………………………33 【圖4.1.7】35mj與其各密度對毛髮周期的影響…………………………………33 【圖4.1.8】長出毛髮面積隨時間變化的百分比(□●) …………………………36 【圖 4.2.1】15mJ-1048spot/cm2模式隨時間毛髮週期變化………………………28 【圖4.3.1】15mJ-1048spot/cm2模式隨時間長出毛髮百分比……………………40 【圖4.3.2】15mJ-1048spot/cm2模式隨時間長出毛髮長度………………………40 【圖4.3.3】15mJ-1048spot/cm2模式隨時間表皮厚度……………………………40 【圖4.4.1】K15雙染BrdU觀察毛囊生長週期的變化 …………………………42 【圖4.4.2】P-Cadherin雙染BrdU觀察毛囊生長週期的變化……………………42 【圖4.4.3】Lef-1 mRNA與蛋白表現量……………………………………………44 【圖4.4.4】Wnt3a mRNA表現量………………………………………………… 44 【圖4.5.1】Ly-6G mRNA與蛋白表現量 …………………………………………46 【圖4.5.2】F4/80 mRNA與蛋白表現量 …………………………………………47 【圖4.5.3】TNF-α mRNA與蛋白表現量 …………………………………………49 【圖4.5.4】IL-1β mRNA與蛋白表現量、IL-1α、IL-1Ra mRNA表現量………51 【圖4.5.5】IL-1R1 mRNA與蛋白表現量…………………………………………52 【圖4.5.6】NF-κB 蛋白表現量……………………………………………………53 【圖4.5.7】VEGF mRNA與蛋白表現量………………………………………… 55 【圖4.6.1】去除嗜中性白血球對毛囊再生模式的影響 …………………………57 【圖4.6.2】抑制掉TNF-α的表現對毛囊提前進入生長期的影響………………57 【圖4.6.3】阻斷IL-1信號對於毛囊提前進入生長期的影響……………………58 【圖4.6.4】阻斷IL-1信號與抑制TNF-α表現對毛囊提前進入生長期的影響…58 【圖4.6.5】VEGF抑制劑(0.8mg/day)對毛囊提前進入生長期的影響……………60 【圖4.6.6】VEGF抑制劑(1.6mg/day)對毛囊提前進入生長期的影響……………60 【圖4.7.1】發炎相關基因mRNA表現……………………………………………63 【圖4.7.2】發炎相關基因mRNA表現……………………………………………66 【圖4.7.3】發炎相關基因mRNA表現……………………………………………66 表目錄 【表3.3】抗體表……………………………………………………………………17 【表3.6】引子表……………………………………………………………………20 【表 4.1.1】雷射能量密度範圍……………………………………………………23 【表4.1.2】C57BL/6鼠毛囊週期 …………………………………………………23 【表4.1.3】5mj與其各密度 ………………………………………………………25 【表4.1.4】10mj與其各密度………………………………………………………26 【表4.1.5】12mj與其各密度………………………………………………………28 【表4.1.6】15mj與其各密度………………………………………………………29 【表4.1.7】20mj與其各密度………………………………………………………31 【表4.1.8】25mj與其各密度………………………………………………………33 【表4.1.9】35mj與其各密度………………………………………………………33 【表4.1.10】各能量(mJ)與密度(pots/cm2)對毛髮周期的影響……………………36 | |
| dc.language.iso | zh-TW | |
| dc.subject | 毛囊再生 | zh_TW |
| dc.subject | 毛髮生長週期 | zh_TW |
| dc.subject | 1550奈米點陣鉺玻璃雷射 | zh_TW |
| dc.subject | VEGF receptor | zh_TW |
| dc.subject | 禿髮 | zh_TW |
| dc.subject | alopecia | en |
| dc.subject | hair follicle regeneration | en |
| dc.subject | 550-nm fractional erbium glass laser | en |
| dc.subject | micro-injury | en |
| dc.subject | vascular endothelial growth factor | en |
| dc.title | 微傷口對毛髮週期的影響 | zh_TW |
| dc.title | The Effect of Micro-Injury on Hair Cycle | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 102-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 王莉芳(Li-Fang Wang),林國儀(Kuo-I Lin) | |
| dc.subject.keyword | 毛囊再生,毛髮生長週期,1550奈米點陣鉺玻璃雷射,VEGF receptor,禿髮, | zh_TW |
| dc.subject.keyword | alopecia,hair follicle regeneration,1,550-nm fractional erbium glass laser,micro-injury,vascular endothelial growth factor, | en |
| dc.relation.page | 77 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2014-08-14 | |
| dc.contributor.author-college | 工學院 | zh_TW |
| dc.contributor.author-dept | 醫學工程學研究所 | zh_TW |
| 顯示於系所單位： | 醫學工程學研究所 | |
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