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標題: | 合併補充益生菌及含魚油之綜合營養劑對BALB/c小鼠腸道功能的影響 The combined effects of probiotics and nutrition supplement containing fish oil on intestinal function in BALB/c Mice |
作者: | Hou-Chih Chiang 江厚志 |
指導教授: | 林璧鳳(Bi-Fong Lin) |
關鍵字: | 消化酵素活性,益生菌,魚油,比菲德氏菌,乳酸菌,產氣夾膜梭菌, Intestinal digestive enzyme,probiotics,fish oil,Bifidobacteium spp.,Lactobacillus spp.,Clostridium perfringens, |
出版年 : | 2014 |
學位: | 碩士 |
摘要: | 腸道的主要功能是將所攝入的食物進行消化與吸收,而近幾年的研究則發現,人體腸道中的共生菌所組成的腸道菌相,在人體的健康狀態上扮演了重要的角色。
本研究以雀巢公司的益生菌粉與含有魚油的綜合營養劑來管餵小鼠,探討單獨與合併補充對腸道消化酵素活性以及腸道菌相的影響,並參考另外兩位同學所進行的免疫調節及腸道免疫的結果來評估哪種補充方式最佳。非特異性免疫小鼠實驗腸道酵素活性的部分顯示,給予補充皆可以顯著性的提高麥芽糖酶的活性,乳糖酶則是在給予單劑補充益生菌時有著顯著性的提升,白胺酸胺基酵素的活性則是有補充都會提高活性,在高劑量時有顯著性的差益,然而在特異性免疫動物實驗的時候則呈現了相反的調控方式。腸內細菌菌相的部分則顯示了,只要有補充,都可以減少腸道中產氣夾膜梭菌並增加乳酸菌的含量。單獨補充益生菌粉或中、高劑量的合併補充時有較好的效果,僅給予綜合營養劑的補充或是減半劑量的合併補充則沒有效果。 兩批腸道酵素活性實驗的結果不一樣,可能的原因是由於特異性免疫動物實驗需要額外進行腹腔注射OVA,因而導致小鼠腸道功能的受損而造成兩次實驗的結果不一致,因此推論第一批動物實驗的酵素實驗結果較可信。綜合兩次實驗的結果,給予中或高劑量的合併補充時皆會有良好的補充效益,而在給予中劑量的合併補充即可有最好的補充效益。 The main function of the gastrointestinal function was to digest and absorb the food we eat. Recent studies have shown that microbiota which was composed by the intestinal commensal bacteria played an important role in health In this research, we supplied the mice with probiotics and fish oil-containing nutritional supplement through tube feeding to evaluate the effect of single dose or combination of two doses on intestinal digestive enzyme activity and microbiota. And to determine which method will have the best supplemental benefit. The result of intestinal enzyme activity showed that all of the supplement could enhance the activity of maltase and leucine aminopeptidase, and the single dose of probiotic supplement could enhance the activity of lactase in non-specific immune response in mice. But the regulation role of supplement was adverse in OVA-specific immune responses in mice. The microbial result showed that no matter how the supplement we feed the mice, all of them can decrease the Clostridium Perfringens and increase the Lactobacillus spp.. Supply with probiotics and median or high dose combination can increase the bifidobacterium spp. in microbiota. Different result in intestinal enzyme activity may due to the intestinal dysfunction in OVA-specific immune responses in mice which had been intraperitoneal injection of . OVA. Thus, the result of regulation of supplement on intestinal enzyme activity in non-specific immune responses mice may be more dependable. A summary of the result above, we suggested that the supply of the median dose of combination of probiotics and fish oil-containing nutritional supplements may have the better supplementary effect compared to that of other supply method. |
URI: | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/56389 |
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顯示於系所單位: | 生化科技學系 |
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