請用此 Handle URI 來引用此文件:
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/56179
完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 靳宗洛(Tsung-Luo Jinn) | |
dc.contributor.author | Chung-Yen Niu | en |
dc.contributor.author | 牛中言 | zh_TW |
dc.date.accessioned | 2021-06-16T05:17:58Z | - |
dc.date.available | 2016-08-25 | |
dc.date.copyright | 2014-08-25 | |
dc.date.issued | 2014 | |
dc.date.submitted | 2014-08-17 | |
dc.identifier.citation | Abe, H., Urao, T., Ito, T., Seki, M., Shinozaki, K., and Yamaguchi-Shinozaki, K. (2003). Arabidopsis AtMYC2 (bHLH) and AtMYB2 (MYB) function as transcriptional activators in abscisic acid signaling. Plant Cell 15: 63-78.
Abebe, T., Guenzi, A.C., Martin, B., and Cushman, J.C. (2003). Tolerance of mannitol-accumulating transgenic wheat to water stress and salinity. Plant Physiol 131: 1748-1755. Baniwal, S.K., Chan, K.Y., Scharf, K.D., and Nover, L. (2007). Role of heat stress transcription factor HsfA5 as specific repressor of HsfA4. J Biol Chem 282: 3605-3613. Banti, V., Mafessoni, F., Loreti, E., Alpi, A., and Perata, P. (2010). The heat-inducible transcription factor HsfA2 enhances anoxia tolerance in Arabidopsis. Plant Physiol 152: 1471-1483. Barrero, J.M., Rodriguez, P.L., Quesada, V., Piqueras, P., Ponce, M.R., and Micol, J.L. (2006). Both abscisic acid (ABA)-dependent and ABA-independent pathways govern the induction of NCED3, AAO3 and ABA1 in response to salt stress. Plant Cell Environ 29: 2000-2008. Battisti, D.S., and Naylor, R.L. (2009). Historical warnings of future food insecurity with unprecedented seasonal heat. Science 323: 240-244. Boscheinen, O., Lyck, R., Queitsch, C., Treuter, E., Zimarino, V., and Scharf, K.D. (1997). Heat stress transcription factors from tomato can functionally replace HSF1 in the yeast Saccharomyces cerevisiae. Mol Gen Genet 255: 322-331. Busch, W., Wunderlich, M., and Schoffl, F. (2005). Identification of novel heat shock factor-dependent genes and biochemical pathways in Arabidopsis thaliana. Plant J 41: 1-14. Busk, P.K., and Pages, M. (1998). Regulation of abscisic acid-induced transcription. Plant Mol Biol 37: 425-435. Chang, W.C., Lee, T.Y., Huang, H.D., Huang, H.Y., and Pan, R.L. (2008). PlantPAN: Plant promoter analysis navigator, for identifying combinatorial cis-regulatory elements with distance constraint in plant gene groups. BMC Genomics 9: 561. Charng, Y.Y., Liu, H.C., Liu, N.Y., Hsu, F.C., and Ko, S.S. (2006). Arabidopsis Hsa32, a novel heat shock protein, is essential for acquired thermotolerance during long recovery after acclimation. Plant Physiol 140: 1297-1305. Charng, Y.Y., Liu, H.C., Liu, N.Y., Chi, W.T., Wang, C.N., Chang, S.H., and Wang, T.T. (2007). A heat-inducible transcription factor, HsfA2, is required for extension of acquired thermotolerance in Arabidopsis. Plant Physiol 143: 251-262. Chiu, R.S., Nahal, H., Provart, N.J., and Gazzarrini, S. (2012). The role of the Arabidopsis FUSCA3 transcription factor during inhibition of seed germination at high temperature. BMC Plant Biol 12: 15. Chu, C.C., Lee, W.C., Guo, W.Y., Pan, S.M., Chen, L.J., Li, H.M., and Jinn, T.L. (2005). A copper chaperone for superoxide dismutase that confers three types of copper/zinc superoxide dismutase activity in Arabidopsis. Plant Physiol 139: 425-436. Clough, S.J., and Bent, A.F. (1998). Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana. Plant J 16: 735-743. Czarnecka-Verner, E., Pan, S., Salem, T., and Gurley, W.B. (2004). Plant class B HSFs inhibit transcription and exhibit affinity for TFIIB and TBP. Plant Mol Biol 56: 57-75. Czarnecka-Verner, E., Yuan, C.X., Scharf, K.D., Englich, G., and Gurley, W.B. (2000). Plants contain a novel multi-member class of heat shock factors without transcriptional activator potential. Plant Mol Biol 43: 459-471. Czechowski, T., Stitt, M., Altmann, T., Udvardi, M.K., and Scheible, W.R. (2005). Genome-wide identification and testing of superior reference genes for transcript normalization in Arabidopsis. Plant Physiol 139: 5-17. Davletova, S., Rizhsky, L., Liang, H., Shengqiang, Z., Oliver, D.J., Coutu, J., Shulaev, V., Schlauch, K., and Mittler, R. (2005). Cytosolic ascorbate peroxidase 1 is a central component of the reactive oxygen gene network of Arabidopsis. Plant Cell 17: 268-281. Ehlert, A., Weltmeier, F., Wang, X., Mayer, C.S., Smeekens, S., Vicente-Carbajosa, J., and Droge-Laser, W. (2006). Two-hybrid protein-protein interaction analysis in Arabidopsis protoplasts: establishment of a heterodimerization map of group C and group S bZIP transcription factors. Plant J 46: 890-900. Fortunati, A., Piconese, S., Tassone, P., Ferrari, S., and Migliaccio, F. (2008). A new mutant of Arabidopsis disturbed in its roots, right-handed slanting, and gravitropism defines a gene that encodes a heat-shock factor. J Exp Bot 59: 1363-1374. Frank, G., Pressman, E., Ophir, R., Althan, L., Shaked, R., Freedman, M., Shen, S., and Firon, N. (2009). Transcriptional profiling of maturing tomato (Solanum lycopersicum L.) microspores reveals the involvement of heat shock proteins, ROS scavengers, hormones, and sugars in the heat stress response. J Exp Bot 60: 3891-3908. Fujita, Y., Fujita, M., Shinozaki, K., and Yamaguchi-Shinozaki, K. (2011). ABA-mediated transcriptional regulation in response to osmotic stress in plants. J Plant Res 124: 509-525. Haake, V., Cook, D., Riechmann, J.L., Pineda, O., Thomashow, M.F., and Zhang, J.Z. (2002). Transcription factor CBF4 is a regulator of drought adaptation in Arabidopsis. Plant Physiol 130: 639-648. Hancock, K.R., Phillips, L.D., White, D.W., and Ealing, P.M. (1997). pPE1000: a versatile vector for the expression of epitope-tagged foreign proteins in transgenic plants. Biotechniques 22: 861-862, 865. Hiratsu, K., Matsui, K., Koyama, T., and Ohme-Takagi, M. (2003). Dominant repression of target genes by chimeric repressors that include the EAR motif, a repression domain, in Arabidopsis. Plant J 34: 733-739. Hong, S.W., and Vierling, E. (2000). Mutants of Arabidopsis thaliana defective in the acquisition of tolerance to high temperature stress. Proc Natl Acad Sci U S A 97: 4392-4397. Hsu, S.F., Lai, H.C., and Jinn, T.L. (2010). Cytosol-localized heat shock factor-binding protein, AtHSBP, functions as a negative regulator of heat shock response by translocation to the nucleus and is required for seed development in Arabidopsis. Plant Physiol 153: 773-784. Huang, C.H., Kuo, W.Y., Weiss, C., and Jinn, T.L. (2012a). Copper chaperone-dependent and -independent activation of three copper-zinc superoxide dismutase homologs localized in different cellular compartments in Arabidopsis. Plant Physiol 158: 737-746. Huang, G.T., Ma, S.L., Bai, L.P., Zhang, L., Ma, H., Jia, P., Liu, J., Zhong, M., and Guo, Z.F. (2012b). Signal transduction during cold, salt, and drought stresses in plants. Mol Biol Rep 39: 969-987. Hwang, J.E., Lim, C.J., Chen, H., Je, J., Song, C., and Lim, C.O. (2012). Overexpression of Arabidopsis dehydration- responsive element-binding protein 2C confers tolerance to oxidative stress. Mol Cells 33: 135-140. Hwang, S.M., Kim, D.W., Woo, M.S., Jeong, H.S., Son, Y.S., Akhter, S., Choi, G.J., and Bahk, J.D. (2014). Functional characterization of Arabidopsis HsfA6a as a heat-shock transcription factor under high salinity and dehydration conditions. Plant Cell Environ 37: 1202-1222. Ikeda, M., and Ohme-Takagi, M. (2009). A novel group of transcriptional repressors in Arabidopsis. Plant Cell Physiol 50: 970-975. Ikeda, M., Mitsuda, N., and Ohme-Takagi, M. (2011). Arabidopsis HsfB1 and HsfB2b act as repressors of the expression of heat-inducible Hsfs but positively regulate the acquired thermotolerance. Plant Physiol 157: 1243-1254. Jung, H.S., Crisp, P.A., Estavillo, G.M., Cole, B., Hong, F., Mockler, T.C., Pogson, B.J., and Chory, J. (2013). Subset of heat-shock transcription factors required for the early response of Arabidopsis to excess light. Proc Natl Acad Sci U S A 110: 14474-14479. Karpinski, S., Reynolds, H., Karpinska, B., Wingsle, G., Creissen, G., and Mullineaux, P. (1999). Systemic signaling and acclimation in response to excess excitation energy in Arabidopsis. Science 284: 654-657. Kim, J.S., Mizoi, J., Yoshida, T., Fujita, Y., Nakajima, J., Ohori, T., Todaka, D., Nakashima, K., Hirayama, T., Shinozaki, K., and Yamaguchi-Shinozaki, K. (2011). An ABRE promoter sequence is involved in osmotic stress-responsive expression of the DREB2A gene, which encodes a transcription factor regulating drought-inducible genes in Arabidopsis. Plant Cell Physiol 52: 2136-2146. Kim, S., Kang, J.Y., Cho, D.I., Park, J.H., and Kim, S.Y. (2004). ABF2, an ABRE-binding bZIP factor, is an essential component of glucose signaling and its overexpression affects multiple stress tolerance. Plant J 40: 75-87. Kotak, S., Vierling, E., Baumlein, H., and von Koskull-Doring, P. (2007a). A novel transcriptional cascade regulating expression of heat stress proteins during seed development of Arabidopsis. Plant Cell 19: 182-195. Kotak, S., Port, M., Ganguli, A., Bicker, F., and von Koskull-Doring, P. (2004). Characterization of C-terminal domains of Arabidopsis heat stress transcription factors (Hsfs) and identification of a new signature combination of plant class A Hsfs with AHA and NES motifs essential for activator function and intracellular localization. Plant J 39: 98-112. Kotak, S., Larkindale, J., Lee, U., von Koskull-Doring, P., Vierling, E., and Scharf, K.D. (2007b). Complexity of the heat stress response in plants. Curr Opin Plant Biol 10: 310-316. Krishna P (2004) Plant responses to heat stress. In H Hirt, K Shinozaki, eds, Plant Responses to Abiotic Stress, Vol 4. Springer Berlin Heidelberg, pp 73-101 Larkindale, J., and Knight, M.R. (2002). Protection against heat stress-induced oxidative damage in arabidopsis involves calcium, abscisic acid, ethylene, and salicylic acid. Plant Physiol 128: 682-695. Larkindale, J., and Huang, B. (2004). Thermotolerance and antioxidant systems in Agrostis stolonifera: involvement of salicylic acid, abscisic acid, calcium, hydrogen peroxide, and ethylene. J Plant Physiol 161: 405-413. Larkindale, J., Hall, J.D., Knight, M.R., and Vierling, E. (2005). Heat stress phenotypes of Arabidopsis mutants implicate multiple signaling pathways in the acquisition of thermotolerance. Plant Physiol 138: 882-897. Lim, C.J., Hwang, J.E., Chen, H., Hong, J.K., Yang, K.A., Choi, M.S., Lee, K.O., Chung, W.S., Lee, S.Y., and Lim, C.O. (2007). Over-expression of the Arabidopsis DRE/CRT-binding transcription factor DREB2C enhances thermotolerance. Biochem Biophys Res Commun 362: 431-436. Liu, H.C., and Charng, Y.Y. (2013). Common and distinct functions of Arabidopsis class A1 and A2 heat shock factors in diverse abiotic stress responses and development. Plant Physiol 163: 276-290. Liu, H.C., Liao, H.T., and Charng, Y.Y. (2011). The role of class A1 heat shock factors (HSFA1s) in response to heat and other stresses in Arabidopsis. Plant Cell Environ 34: 738-751. Lohmann, C., Eggers-Schumacher, G., Wunderlich, M., and Schoffl, F. (2004). Two different heat shock transcription factors regulate immediate early expression of stress genes in Arabidopsis. Mol Genet Genomics 271: 11-21. Lu, Q., Tang, X., Tian, G., Wang, F., Liu, K., Nguyen, V., Kohalmi, S.E., Keller, W.A., Tsang, E.W., Harada, J.J., Rothstein, S.J., and Cui, Y. (2010). Arabidopsis homolog of the yeast TREX-2 mRNA export complex: components and anchoring nucleoporin. Plant J 61: 259-270. Maere, S., Heymans, K., and Kuiper, M. (2005). BiNGO: a Cytoscape plugin to assess overrepresentation of gene ontology categories in biological networks. Bioinformatics 21: 3448-3449. Mishra, S.K., Tripp, J., Winkelhaus, S., Tschiersch, B., Theres, K., Nover, L., and Scharf, K.D. (2002). In the complex family of heat stress transcription factors, HsfA1 has a unique role as master regulator of thermotolerance in tomato. Genes Dev 16: 1555-1567. Mittler, R. (2006). Abiotic stress, the field environment and stress combination. Trends Plant Sci 11: 15-19. Nakashima, K., and Yamaguchi-Shinozaki, K. (2013). ABA signaling in stress-response and seed development. Plant Cell Rep 32: 959-970. Nambara, E., Keith, K., McCourt, P., and Naito, S. (1994). Isolation of an internal deletion mutant of the Arabidopsis thaliana ABI3 gene. Plant Cell Physiol 35: 509-513. Narusaka, Y., Nakashima, K., Shinwari, Z.K., Sakuma, Y., Furihata, T., Abe, H., Narusaka, M., Shinozaki, K., and Yamaguchi-Shinozaki, K. (2003). Interaction between two cis-acting elements, ABRE and DRE, in ABA-dependent expression of Arabidopsis rd29A gene in response to dehydration and high-salinity stresses. Plant J 34: 137-148. Neff, M.M., and Chory, J. (1998). Genetic interactions between phytochrome A, phytochrome B, and cryptochrome 1 during Arabidopsis development. Plant Physiol 118: 27-35. Nishizawa, A., Yabuta, Y., Yoshida, E., Maruta, T., Yoshimura, K., and Shigeoka, S. (2006). Arabidopsis heat shock transcription factor A2 as a key regulator in response to several types of environmental stress. Plant J 48: 535-547. Nover, L., Bharti, K., Doring, P., Mishra, S.K., Ganguli, A., and Scharf, K.D. (2001). Arabidopsis and the heat stress transcription factor world: how many heat stress transcription factors do we need? Cell Stress Chaperones 6: 177-189. Ogawa, D., Yamaguchi, K., and Nishiuchi, T. (2007). High-level overexpression of the Arabidopsis HsfA2 gene confers not only increased themotolerance but also salt/osmotic stress tolerance and enhanced callus growth. J Exp Bot 58: 3373-3383. Panchuk, II, Volkov, R.A., and Schoffl, F. (2002). Heat stress- and heat shock transcription factor-dependent expression and activity of ascorbate peroxidase in Arabidopsis. Plant Physiol 129: 838-853. Parsell, D.A., and Lindquist, S. (1993). The function of heat-shock proteins in stress tolerance: degradation and reactivation of damaged proteins. Annu Rev Genet 27: 437-496. Perez-Salamo, I., Papdi, C., Rigo, G., Zsigmond, L., Vilela, B., Lumbreras, V., Nagy, I., Horvath, B., Domoki, M., Darula, Z., Medzihradszky, K., Bogre, L., Koncz, C., and Szabados, L. (2014). The heat shock factor A4A confers salt tolerance and is regulated by oxidative stress and the mitogen-activated protein kinases MPK3 and MPK6. Plant Physiol 165: 319-334. Pogson, B.J., Woo, N.S., Forster, B., and Small, I.D. (2008). Plastid signalling to the nucleus and beyond. Trends Plant Sci 13: 602-609. Porra, R.J., Thompson, W.A., and Kriedemann, P.E. (1989). Determination of accurate extinction coefficients and simultaneous equations for assaying chlorophylls a and b extracted with four different solvents: verification of the concentration of chlorophyll standards by atomic absorption spectroscopy. Biochim Biophys Acta 975: 384-394. Qin, F., Shinozaki, K., and Yamaguchi-Shinozaki, K. (2011). Achievements and challenges in understanding plant abiotic stress responses and tolerance. Plant Cell Physiol 52: 1569-1582. Rabindran, S.K., Haroun, R.I., Clos, J., Wisniewski, J., and Wu, C. (1993). Regulation of heat shock factor trimer formation: role of a conserved leucine zipper. Science 259: 230-234. Rossel, J.B., Walter, P.B., Hendrickson, L., Chow, W.S., Poole, A., Mullineaux, P.M., and Pogson, B.J. (2006). A mutation affecting ASCORBATE PEROXIDASE 2 gene expression reveals a link between responses to high light and drought tolerance. Plant Cell Environ 29: 269-281. Sakuma, Y., Maruyama, K., Qin, F., Osakabe, Y., Shinozaki, K., and Yamaguchi-Shinozaki, K. (2006). Dual function of an Arabidopsis transcription factor DREB2A in water-stress-responsive and heat-stress-responsive gene expression. Proc Natl Acad Sci U S A 103: 18822-18827. Scharf, K.D., Berberich, T., Ebersberger, I., and Nover, L. (2012). The plant heat stress transcription factor (Hsf) family: structure, function and evolution. Biochim Biophys Acta 1819: 104-119. Schmidt, R., Schippers, J.H., Welker, A., Mieulet, D., Guiderdoni, E., and Mueller-Roeber, B. (2012). Transcription factor OsHsfC1b regulates salt tolerance and development in Oryza sativa ssp. japonica. AoB Plants 2012: pls011. Schoffl, F., Prandl, R., and Reindl, A. (1998). Regulation of the heat-shock response. Plant Physiol 117: 1135-1141. Schramm, F., Ganguli, A., Kiehlmann, E., Englich, G., Walch, D., and von Koskull-Doring, P. (2006). The heat stress transcription factor HsfA2 serves as a regulatory amplifier of a subset of genes in the heat stress response in Arabidopsis. Plant Mol Biol 60: 759-772. Schramm, F., Larkindale, J., Kiehlmann, E., Ganguli, A., Englich, G., Vierling, E., and von Koskull-Doring, P. (2008). A cascade of transcription factor DREB2A and heat stress transcription factor HsfA3 regulates the heat stress response of Arabidopsis. Plant J 53: 264-274. Shi, W.M., Muramoto, Y., Ueda, A., and Takabe, T. (2001). Cloning of peroxisomal ascorbate peroxidase gene from barley and enhanced thermotolerance by overexpressing in Arabidopsis thaliana. Gene 273: 23-27. Shinozaki, K., and Yamaguchi-Shinozaki, K. (2000). Molecular responses to dehydration and low temperature: differences and cross-talk between two stress signaling pathways. Curr Opin Plant Biol 3: 217-223. Siddique, M., Gernhard, S., von Koskull-Doring, P., Vierling, E., and Scharf, K.D. (2008). The plant sHSP superfamily: five new members in Arabidopsis thaliana with unexpected properties. Cell Stress Chaperones 13: 183-197. Suzuki, N., Sejima, H., Tam, R., Schlauch, K., and Mittler, R. (2011). Identification of the MBF1 heat-response regulon of Arabidopsis thaliana. Plant J 66: 844-851. Suzuki, N., Miller, G., Sejima, H., Harper, J., and Mittler, R. (2013). Enhanced seed production under prolonged heat stress conditions in Arabidopsis thaliana plants deficient in cytosolic ascorbate peroxidase 2. J Exp Bot 64: 253-263. Swindell, W.R., Huebner, M., and Weber, A.P. (2007). Transcriptional profiling of Arabidopsis heat shock proteins and transcription factors reveals extensive overlap between heat and non-heat stress response pathways. BMC Genomics 8: 125. Thordal-Christensen, H., Zhang, Z., Wei, Y., and Collinge, D.B. (1997). Subcellular localization of H2O2 in plants. H2O2 accumulation in papillae and hypersensitive response during the barley-powdery mildew interaction. Plant J 11: 1187-1194. Vierling, E. (1991). The Roles of Heat-Shock Proteins in Plants. Annu Rev Plant Physiol Plant Mol Biol 42: 579-620. von Koskull-Doring, P., Scharf, K.D., and Nover, L. (2007). The diversity of plant heat stress transcription factors. Trends Plant Sci 12: 452-457. Waters, E.R., Aevermann, B.D., and Sanders-Reed, Z. (2008). Comparative analysis of the small heat shock proteins in three angiosperm genomes identifies new subfamilies and reveals diverse evolutionary patterns. Cell Stress Chaperones 13: 127-142. Wunderlich, M., Groß-Hardt, R., and Schöffl, F. (2014). Heat shock factor HSFB2a involved in gametophyte development of Arabidopsis thaliana and its expression is controlled by a heat-inducible long non-coding antisense RNA. Plant Mol Biol: doi: 10.1007/s11103-11014-10202-11100. Yokotani, N., Ichikawa, T., Kondou, Y., Matsui, M., Hirochika, H., Iwabuchi, M., and Oda, K. (2008). Expression of rice heat stress transcription factor OsHsfA2e enhances tolerance to environmental stresses in transgenic Arabidopsis. Planta 227: 957-967. Yoo, S.D., Cho, Y.H., and Sheen, J. (2007). Arabidopsis mesophyll protoplasts: a versatile cell system for transient gene expression analysis. Nat Protoc 2: 1565-1572. Yoshida, T., Fujita, Y., Sayama, H., Kidokoro, S., Maruyama, K., Mizoi, J., Shinozaki, K., and Yamaguchi-Shinozaki, K. (2010). AREB1, AREB2, and ABF3 are master transcription factors that cooperatively regulate ABRE-dependent ABA signaling involved in drought stress tolerance and require ABA for full activation. Plant J 61: 672-685. Yoshida, T., Sakuma, Y., Todaka, D., Maruyama, K., Qin, F., Mizoi, J., Kidokoro, S., Fujita, Y., Shinozaki, K., and Yamaguchi-Shinozaki, K. (2008). Functional analysis of an Arabidopsis heat-shock transcription factor HsfA3 in the transcriptional cascade downstream of the DREB2A stress-regulatory system. Biochem Biophys Res Commun 368: 515-521. Yoshida, T., Ohama, N., Nakajima, J., Kidokoro, S., Mizoi, J., Nakashima, K., Maruyama, K., Kim, J.M., Seki, M., Todaka, D., Osakabe, Y., Sakuma, Y., Schoffl, F., Shinozaki, K., and Yamaguchi-Shinozaki, K. (2011). Arabidopsis HsfA1 transcription factors function as the main positive regulators in heat shock-responsive gene expression. Mol Genet Genomics 286: 321-332. Zimmermann, P., Hirsch-Hoffmann, M., Hennig, L., and Gruissem, W. (2004). GENEVESTIGATOR. Arabidopsis microarray database and analysis toolbox. Plant Physiol 136: 2621-2632. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/56179 | - |
dc.description.abstract | 熱休克反應是一個對抗熱逆境的重要機制,其可以藉由熱休克轉錄因子的調控增加熱休克蛋白的表現。此外藉由植物逆境賀爾蒙離層酸 (ABA) 的訊息傳遞也會參與在後天耐熱性。分析微陣列晶片中的資料顯示,阿拉伯芥熱休克轉錄因子HSFA6b會被鹽、滲透壓和冷逆境誘導表現而不是在熱逆境。在本篇文章中我們確定了HSFA6b會參與在ABA訊息傳遞並且會提升熱耐受性。有趣的是,在先前的研究中顯示在ABA缺失及對ABA不敏感的突變體中,證實ABA相關的訊息傳遞對於HSFA6b的表現是必須的;藉此我們也利用在原生質體中的轉錄活性檢測,確定了HSFA6b啟動子中的ABRE (ABA反應序列) 對ABA和AREB1 (ABA反應結合蛋白1) 的感應是不可或缺的,除此之外DREB2A (乾旱反應序列結合蛋白2A)、HSP18.2 (熱休克蛋白18.2) 和APX2 (抗壞血酸過氧化物酶2) 也會受HSFA6b所調控。而利用HSFA6b的轉植株及其突變體進行實驗,我們也發現HSFA6b會正向調控參與在ABA媒介的鹽和乾旱的耐受性。在耐熱性測試的結果也顯示HSFA6b會參與在後天耐熱性的調控過程中。我們的研究結果說明了一條新的調控途徑,HSFA6b除了會參與在ABA媒介的逆境反應之外也會參與在熱逆境調控的複雜網路之中。 | zh_TW |
dc.description.abstract | The heat stress (HS) response (HSR) is a conserved mechanism developed to increase the expression of heat shock proteins (HSPs) via a heat shock factor (HSF)-dependent mechanism. As well, signaling by the stress phytohormone abscisic acid (ABA) is involved in acquired thermotolerance. Analysis of Arabidopsis (Arabidopsis thaliana) microarray databases revealed that the expression of HSFA6b, a class-A HSF, was increased with salinity, osmotic and cold but not HS. Here, we show that HSFA6b plays a pivotal role in the response to ABA and in thermotolerance in Arabidopsis. Intriguingly, in previous studies showed salt-inducible HSFA6b expression was downregulated in ABA-insensitive and -deficient mutants, and exogenous ABA application restored its expression in ABA-deficient plants, so the ABA signal is required for proper HSFA6b expression. Consequently, transcriptional activation assay of protoplasts showed that ABA treatment and coexpression of an ABA-signaling master effector, ABRE-binding protein 1 (AREB1), could activate the HSFA6b promoter. In addition, DREB2A, HSP18.2 and APX2 were regulated by heat shock factor A6b that enhanced their expression. Analysis of ABA responses in drought and salt tolerance in HSFA6b-null, -overexpression and -dominant–negative mutants indicated that HSFA6b is a positive regulator participating in ABA-mediated salt and drought resistance. Thermoprotection tests showed that HSFA6b was required for thermotolerance acquisition. Our study reveals a network in which HSFA6b operates as a downstream regulator of the ABA-mediated stress response and is required for HS resistance. This new ABA-signaling pathway is integrated into the complex HSR network in planta. | en |
dc.description.provenance | Made available in DSpace on 2021-06-16T05:17:58Z (GMT). No. of bitstreams: 1 ntu-103-R00b42023-1.pdf: 4057340 bytes, checksum: c9064fab205d3d4bd275f6c27dd9da5d (MD5) Previous issue date: 2014 | en |
dc.description.tableofcontents | 摘要 I
Abstract II Introduction 1 Heat Shock Response and Thermotolerance 1 Heat Shock Factors 2 Heat Shock Factors in Arabidopsis 2 ABA signaling and mediates thermotolerance 4 Oxidative stress gene APX2 5 Motivation and Objectives 6 Materials and Methods 7 Plant Materials and Growth Conditions 7 RNA Preparation, cDNA Synthesis and Real-Time Quantitative PCR 7 Generation of Transgenic HSFA6b Mutant Plants 7 Bimolecular Fluorescence Complementation (BiFC) assays 8 Protoplast Transactivation Assay 8 Thermotolerance, Superoxide Dismutase (SOD) Activity, H2O2 and Pigment Content Determination 8 Chromatin Immunoprecipitation (ChIP) Assay 9 Microarray Assay 9 Statistical analysis 10 Primers and Accession Numbers 10 Results 11 Arabidopsis Class-A HSF HSFA6b Is Induced by Salt, Osmotic and ABA Treatment, not by HS 11 ABA Treatment and AREB1/ABF2 Co-expression Enhanced HSFA6b Promoter Activity 11 HSFA6b-null, -OE and -dominant–negative Mutants Were Screened and Confirmed 12 Misregulated of ABA-biosynthesis and -responsive Gene Expression in Response to Salt and ABA Treatment in HSFA6b Mutants 12 HSFA6b Participated in Salt- and ABA-Mediated HSP Expression 13 HSFA6b and other HSFs can interact and form hetero- not homo-oligomers 14 HSFA6s double-knockout and HSFA3-null mutants were screened and confirmed 15 HSFA6b functions independently of HSFA6a and with HSFA3 for activation of HSP18.2 and APX2 promoter 15 HSFA6b Regulated Thermotolerance 15 HSFA6b Overexpression Conferred Drought and Salt Tolerance 16 Transcription Profiling of HSFA6b-OE and -RD transgenic plants 16 Discussion 18 HSFA6b, a Nuclear Factor, Responds to ABA Signaling via the ABRE/ABF-ABRE Regulon 18 HSFA6b, a Positive Regulator, Is Involved in ABA-mediated Salt and Osmotic Stress Responses 20 HSFA6b Mediated ABA-Induced Thermo-, Drought- and Salt-tolerance 20 Crosstalk of HSFA6 and DREB2A in HSR Gene Expression and Regulatory Networks 21 There are other HS- transcription factors may co-interaction with HSFA6b under HS 24 HSFA6b may mediated HSFA3 co-interaction required for thermotolerance under diverse stress conditions 24 Tables 25 Figures 32 References 49 Supplemental Data 58 Appendixes 65 | |
dc.language.iso | en | |
dc.title | 阿拉伯芥熱休克轉錄因子HSFA6b作為ABA訊息傳遞和熱休克反應的連結點,扮演正向提升鹽、乾旱以及熱耐受性的角色 | zh_TW |
dc.title | Arabidopsis heat shock transcription factor HSFA6b, with a positive role in salt, drought and heat stress tolerance, is an important node connecting the ABA signal and ABA-mediated heat shock response | en |
dc.type | Thesis | |
dc.date.schoolyear | 102-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 林秋榮,葉開溫,李昆達,張英? | |
dc.subject.keyword | 離層酸,熱休克轉錄因子,熱休克反應, | zh_TW |
dc.subject.keyword | abscisic acid,heat shock factor,heat shock response, | en |
dc.relation.page | 67 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2014-08-17 | |
dc.contributor.author-college | 生命科學院 | zh_TW |
dc.contributor.author-dept | 植物科學研究所 | zh_TW |
顯示於系所單位: | 植物科學研究所 |
文件中的檔案:
檔案 | 大小 | 格式 | |
---|---|---|---|
ntu-103-1.pdf 目前未授權公開取用 | 3.96 MB | Adobe PDF |
系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。