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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 宋延齡(Yen-Ling Song) | |
dc.contributor.author | Chih-Hsuan Teng | en |
dc.contributor.author | 滕致萱 | zh_TW |
dc.date.accessioned | 2021-06-16T05:13:35Z | - |
dc.date.available | 2017-08-25 | |
dc.date.copyright | 2014-08-25 | |
dc.date.issued | 2014 | |
dc.date.submitted | 2014-08-18 | |
dc.identifier.citation | Bishop, G.R., Jaso-Friedmann, L. and Evans, D.L. (2000). Activation-induced programmed cell death of nonspecific cytotoxic cells and inhibition by apoptosis regulatory factors. Cellular Immunology 1, 126-37.
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dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/56034 | - |
dc.description.abstract | 從點帶石斑魚的脾臟選殖出非特異毒殺細胞受器蛋白分子(NCCRP-1) cDNA,包括132 bp 5’端非轉譯區( 5’ untranslated region, 5’ UTR)、699 bp 編碼區 (open reading frame, ORF) 及108 bp 3’UTR,共可轉譯出232個胺基酸。根據Expert Protein Analysis System (ExPASy)網站上的Motif Scan、美國國家生物技術資訊中心 (The National Center for Biotechnology Information, NCBI)的Position-Specific Iterative BLAST進行domain 預測結果顯示,NCCRP-1在第30~228個胺基酸區域為F-box associated (FBA) domain,內含三個功能區塊,分別是N端的proline-rich 區塊、中間的antigen binding 區塊及C端的signal transduction 區塊;但並沒有預測到穿膜區。
NCCRP-1蛋白質分子量推測為26 kDa、等電點是5.06。胺基酸序列多重比對顯示,石斑魚和其他5種海水魚的NCCRP-1一致性在56~84%,和紅笛鯛 (Lutjanus sanguineus)最相近。Q-PCR結果顯示石斑魚受到海水白點蟲感染後第一天NCCRP-1轉錄表現顯著上升,第三天達到最高,第七天降至背景值。利用昆蟲細胞Sf21和桿狀病毒表現系統合成石斑魚NCCRP-1重組蛋白,經SDS-PAGE和西方墨點轉漬法檢驗,分子量約為29 kDa,顯示NCCRP-1 分子經過轉譯後修飾,在Asn171有N-linkage,可能進行醣基化反應;在Gly93及Gly158各有一段N-myristoylation的片段 (GIPAGV及GADKST) ,在Cys10 推測為palmitoylation位置,可能進行脂化反應(Lipidation)。LC Mass/Mass比對重組蛋白確定為石斑魚NCCRP-1 。將NCCRP-1重組蛋白免疫紐西蘭大白兔八週後取得抗血清,硫銨沉澱(部份純化)的免疫球蛋白(Ig)耦合上FITC,進行流式細胞分析周邊及組織內白血球;顯示寄生蟲感染後第四天,細胞膜上RP-1平均表現量(MFI)顯著增加。顯示NCCRP-1是一種可被誘發 (inducible) 的基因,且表現量由低至高。 從流式細胞儀分析及原位雜交皆顯示,石斑魚NCCRP-1+細胞為直徑3-6 μm的白血球。石斑魚受到寄生蟲感染前後,以流式細胞儀測量血液、脾臟、胸腺、鰓、皮膚及頭腎NCCRP-1+細胞佔全部白血球的比例,顯示感染前約佔1%,而感染後則增加到3~19.3%。取石斑魚受海水白點蟲感染後第三天的鰓組織切片,用NCCRP-1 antisense riboprobe進行原位雜交(in situ hybridization),發現海水白點蟲營養體(trophont)周圍及內部有NCCRP-1+細胞。NCCRP-1+細胞具有毒殺海水白點蟲纖毛幼蟲和被神經壞死病毒(nervous necrosis virus, NNV)感染的細胞的能力。 | zh_TW |
dc.description.provenance | Made available in DSpace on 2021-06-16T05:13:35Z (GMT). No. of bitstreams: 1 ntu-103-R00b41010-1.pdf: 4154836 bytes, checksum: 3f81f378b5da62f88ee94c6bb22d8fb3 (MD5) Previous issue date: 2014 | en |
dc.description.tableofcontents | 誌謝 i
摘要 ii Abstract iv 圖表目錄 vii 附表目錄 ix 緒論 1 文獻回顧 2 研究目的 12 材料方法 13 結果 29 討論 34 參考文獻 39 | |
dc.language.iso | zh-TW | |
dc.title | 點帶石斑魚非特異性毒殺細胞受器蛋白分子的選殖與定性 | zh_TW |
dc.title | Molecular Cloning and Characterization of Nonspecific Cytotoxic Cell Receptor Protein-1 (NCCRP-1) from Orange-Spotted Grouper (Epinephelus coioides) | en |
dc.type | Thesis | |
dc.date.schoolyear | 102-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 顧家綺(Chia-Chi Ku),陳俊宏(Jiun-Hong Chen),宋宏紅(Hung-Hung.Sung),沈三泰(San-Tai Shen) | |
dc.subject.keyword | 非特異性毒殺細胞受器蛋白分子,點帶石斑魚,海水白點蟲, | zh_TW |
dc.subject.keyword | NCCRP-1,Orange-Spotted Grouper,Cryptocaryon irritans, | en |
dc.relation.page | 82 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2014-08-18 | |
dc.contributor.author-college | 生命科學院 | zh_TW |
dc.contributor.author-dept | 生命科學系 | zh_TW |
顯示於系所單位: | 生命科學系 |
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ntu-103-1.pdf 目前未授權公開取用 | 4.06 MB | Adobe PDF |
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