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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 廖文亮 | |
dc.contributor.author | Min-Che Tsai | en |
dc.contributor.author | 蔡旻哲 | zh_TW |
dc.date.accessioned | 2021-06-16T05:13:20Z | - |
dc.date.available | 2024-12-31 | |
dc.date.copyright | 2014-08-25 | |
dc.date.issued | 2014 | |
dc.date.submitted | 2014-08-18 | |
dc.identifier.citation | 劉翠玲,陳政忻,2009,石斑魚產業概況及趨勢,農業生技產業季刊,
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Aquaculture 151:283-313 Burel, C., Boujard, T., Kaushik, S.J., Boeuf, G., Van Der Geyten, S., Mol, K. A., Kuhn, E. R. 1998. Incorporation of high level of extruded lupin in diets for rainbow trout (Oncorhynchus mykiss):nutritional value and effect on thyroid status. Aquaculture,188: 363-383. Balcazar J. L., Blas I. D., Zarzuela I. R., 2006. The role of probiotics in aquaculture. Veterinary Microbiology 114: 173-186. Buchet V., Zambonino Infante, J.L., Cahu, C.L.,2000. Effect of lipid level in a compound diet on delvelpment of red drum (Sciaenops ocellatus) larvae. Aquaculture,184:339-347 Cahu, C.L., Zambonino Infante, J.L., Peres, A., Quazuguel, P., Le Gall, M.M., 1998. Algal addition in sea bass (Dicentrarchus labrax) larvae rearing: effect on digestive enzymes. Aquaculture 161: 479-489. Couso, N., Castro, R., Noya M., Obach, A., Lamas, J., 2001. 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P.380. Laining, A., Usman., Kabangnga, N., 2004. The effect of dietary protein level on apparent digest ability coefficient and biological performances of tiger grouper (Epinephelus fuscoguttatus) raised at floating net cage . J. Penelitian Perikanan Indonesia. 9:29-34. Luo, Z., Liu, Y.J., Mai, K.S., Tian, L.X., Tan X.Y., 2004. Op-timal dietary protein requirement of grouper Epinephelus coioides junveniles fed isoenergetic diets in floating net cages. Aquacult.Nutr.10: 247-252. Munilla-Moran, R., Stark, J.R. and Barbour A. The role of exogenous enzymes in digestion in cultured turbot larvae (Scophthalmus maximus L.). Aquaculture. 88 :337-350. Ringo, E., Myklebust, R., Mayhew, T.M., Olsen, R. E., 2007. Bacterial translocation and pathogenesis in the digestive tract of larvae and fry. Aquaculture 268: 251-264. Sarasquete M.C.,1996. Characterization of digestive enzyme activity during larval development of gilthead seabream (Sparus aurata). Fish Physiology and Biochemistry vol. 15 no. 2 pp 121-130. Suzer C., Coban D., Kamaci H. O., Saka Ş., Firat K., Otgucuoglu O.,Kucuksari H., 2008. Lactobacillus spp. bacteria as probiotics in gilthead sea bream (Sparus aurata, L.) larvae: Effects on growth performance and digestive enzyme activities. Aquaculture 280:140-145. Tuan, L.A., Williams, K.C., 2007. Optimum dietary protein and lipid specifiations for junvenile Malabar grouper (Epinephelus malabaricus). Aquaculture 267: 129-138. Watanabe, T., Viyakarn, V., Aoki, H., Tsuda, H., Sakamoto, H., Maita, M., Satoh, S. and Takeuchi, T., 1994. Utilization of alternative protein sources as substitute for fish meal in newly delveloped soft-dry pellet for yellowtail. Suisanzoushoku, 43:511-520 Watanabe, T., Aoki, H., Shimamoto, K., Hadzuma, M., Maita, M., Yamagata, Y., Kiron,V. and Satoh, S. 1998. A trial to culture yellowtail with non-fish meal diets. Fish. Sci., 64: 505-512 Watanabe, T., Aoki, H., Watanabe, K., Maita, M., Yamagata, Y. and Satoh, S., 2001. Quality evaluation of different types of non-fish meal diets for yellowtail. Fish.Sci., 67: 461-469. Whyte S. K., 2007. The innate immune response of finfish - A review of current knowledge. Fish & Shellfish Immunology 23: 1127-1151. Pongmaneerat, J., Watanabe, T., Takeuchi, T. and Satoh, S. 1993. Use of different Protein meals as partial or total substitution for fish meal in carp diets. Nippon Suisan Gakkaishi, 59: 1249-1257. Yano T., 2000. The immune system. In: Chen JC, Cheng SY (eds.) Progress in Fish Immunology. Nation Taiwan Ocean University, Keelung, Taiwan, p 41-61. Zambonino Infante, J. L. and Cahu, C. L.1999. High dietary lipid levels Enhance digestive tract maturation and improve Dicentrarchus labrax laval development J. Nutr.,129 :1195-2000. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/56025 | - |
dc.description.abstract | 本實驗目的在於利用外源性消化酵素添加於以玉米蛋白取代10%魚粉的飼料中,探討對於點帶石斑成長與免疫的影響。實驗中以寒天(Agar)與凝膠蛋白(Gelatin)包覆不同比例的市售天然消化酵素,與無包覆組別的飼料投餵平均體重為4.55±0.55克,每組15尾三重複,飼料中以55%魚粉與10%玉米蛋白做為蛋白來源。實驗結果顯示,就成長與免疫而言,包覆與未包覆之效果皆不影響魚體的成長與免疫。而單以成長討論,消化酵素協助消化吸收玉米蛋白使得結果並無顯著差異,其中以包覆組的添加量0.10%為最適添加量,其增重率為525%。而若以直接添加消化酵素於飼料中,以添加量0.05%為最適添加量,其增重率為531%。
在免疫方面,經過投餵消化酵素飼料後的點帶石斑其膽固醇皆獲得下降,其中以0.15%為最適添加量,其膽固醇皆獲得最大的降低。在提升超氧陰離子產生率的結果顯示,無論有使用寒天和動物膠包覆的組別,抑或是直接添加入消化酵素於飼料當中的組別,溶菌酵素活性與超氧陰離子的產生率,皆比無添加消化酵素的對照組高,結果顯示添加消化酵素後,對於點帶石斑的免疫能力,就溶菌酵素活性與超氧陰離子的產生率而言,皆以添加量為0.15%的組別為最佳。 | zh_TW |
dc.description.abstract | The study aims to investigate the effects of dietary different in encapsulated and unencapsulated nature digestive enzymes on growth and immune responses of Epinephelus coioides. In experiment used agar and gelatin encapsulated with different proportions of natural digestive enzymes and unencapsulated treatment. The diets were fed to triplicate of group of orange-spotted grouper with 4.55±0.55g.
The results indicated that effects of encapsulated and unencapsulated neither affect growth and immune response. Growth of digestive enzymes assist digestion and absorption of corn protein makes no significant difference in results. The terms of encapsulated digestive enzymes in the diets to add the amount of 0.10% is the optimum proportion , weight gain was 525% and the terms of directly adding digestive enzymes in the feed to add the amount of 0.05% is the optimum proportion, weight gain was 531%. The results showed that after adding digestive enzymes, lysozyme activity and the generation rate of superoxide anion, 0.15% is the optimum propotion. | en |
dc.description.provenance | Made available in DSpace on 2021-06-16T05:13:20Z (GMT). No. of bitstreams: 1 ntu-103-R01b45013-1.pdf: 1106305 bytes, checksum: 2fa1ef8f671b0f590c0415383e98f0b5 (MD5) Previous issue date: 2014 | en |
dc.description.tableofcontents | 目錄
中文摘要 i Abstract iv 目錄 v 表目錄 viii 圖目錄 ix 前言 1 一、 全球水產養殖與台灣現況 1 二、 魚種介紹 3 三、 飼料中的蛋白質來源 6 四、 玉米蛋白介紹 7 五、 魚類腸胃道中的消化酶 8 六、 外源性消化酵素 10 七、 魚類的免疫機制 11 八、 血液性狀指標 12 九、 研究目的與動機 13 材料與方法 14 一、試驗魚種與試驗條件 14 二、飼料配製過程 15 三、一般成分分析 15 1. 水份 15 2. 灰分 16 3. 粗脂質分析 16 4. 粗蛋白分析 16 5. 成長分析 17 四、血液生化分析 17 1. 血脂測定 17 2. 膽固醇測定 18 3. 鹼性磷酸酶 18 五、免疫分析 18 1. 全血、血清及肝臟採集 18 2. 分離白血球 19 3.超氧陰離子(O2-)之測定 19 4.溶菌酵素活性之測定 19 六、統計分析 20 結果 21 一、 飼料組成成分 21 二、 成長結果 22 三、 體組成分析 22 四、 血液性狀 23 五、免疫分析 25 討論 27 一、 飼料配方 27 二、 成長效果 28 四、 免疫效果分析 30 結論 31 參考文獻 33 附錄 48 | |
dc.language.iso | zh-TW | |
dc.title | 飼料中添加不同含量的包覆與未包覆天然消化酵素對點帶石斑的成長與免疫之影響 | zh_TW |
dc.title | Effects of dietary different in encapsulated and unencapsulated nature digestive enzymes on growth and immune responses of orange-spotted grouper (Epinephelus coioides) | en |
dc.type | Thesis | |
dc.date.schoolyear | 102-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 張繼堯,丁雲源,葉信利 | |
dc.subject.keyword | 點帶石斑,消化酵素,包覆,玉米蛋白, | zh_TW |
dc.subject.keyword | orange-spotted grouper,corn protein,digestive enzyme,encapsulated, | en |
dc.relation.page | 49 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2014-08-18 | |
dc.contributor.author-college | 生命科學院 | zh_TW |
dc.contributor.author-dept | 漁業科學研究所 | zh_TW |
顯示於系所單位: | 漁業科學研究所 |
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