飼料中添加不同含量的包覆與未包覆天然消化酵素對點帶石斑的成長與免疫之影響

Abstract

本實驗目的在於利用外源性消化酵素添加於以玉米蛋白取代10%魚粉的飼料中，探討對於點帶石斑成長與免疫的影響。實驗中以寒天(Agar)與凝膠蛋白(Gelatin)包覆不同比例的市售天然消化酵素，與無包覆組別的飼料投餵平均體重為4.55±0.55克，每組15尾三重複，飼料中以55%魚粉與10%玉米蛋白做為蛋白來源。實驗結果顯示，就成長與免疫而言，包覆與未包覆之效果皆不影響魚體的成長與免疫。而單以成長討論，消化酵素協助消化吸收玉米蛋白使得結果並無顯著差異，其中以包覆組的添加量0.10%為最適添加量，其增重率為525%。而若以直接添加消化酵素於飼料中，以添加量0.05%為最適添加量，其增重率為531%。 在免疫方面，經過投餵消化酵素飼料後的點帶石斑其膽固醇皆獲得下降，其中以0.15%為最適添加量，其膽固醇皆獲得最大的降低。在提升超氧陰離子產生率的結果顯示，無論有使用寒天和動物膠包覆的組別，抑或是直接添加入消化酵素於飼料當中的組別，溶菌酵素活性與超氧陰離子的產生率，皆比無添加消化酵素的對照組高，結果顯示添加消化酵素後，對於點帶石斑的免疫能力，就溶菌酵素活性與超氧陰離子的產生率而言，皆以添加量為0.15%的組別為最佳。

The study aims to investigate the effects of dietary different in encapsulated and unencapsulated nature digestive enzymes on growth and immune responses of Epinephelus coioides. In experiment used agar and gelatin encapsulated with different proportions of natural digestive enzymes and unencapsulated treatment. The diets were fed to triplicate of group of orange-spotted grouper with 4.55±0.55g. The results indicated that effects of encapsulated and unencapsulated neither affect growth and immune response. Growth of digestive enzymes assist digestion and absorption of corn protein makes no significant difference in results. The terms of encapsulated digestive enzymes in the diets to add the amount of 0.10% is the optimum proportion , weight gain was 525% and the terms of directly adding digestive enzymes in the feed to add the amount of 0.05% is the optimum proportion, weight gain was 531%. The results showed that after adding digestive enzymes, lysozyme activity and the generation rate of superoxide anion, 0.15% is the optimum propotion.