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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 李佳音(Chia-Yin Lee) | |
dc.contributor.author | Hao-Yu Weng | en |
dc.contributor.author | 翁浩瑜 | zh_TW |
dc.date.accessioned | 2021-06-16T05:09:06Z | - |
dc.date.available | 2017-09-09 | |
dc.date.copyright | 2014-09-09 | |
dc.date.issued | 2014 | |
dc.date.submitted | 2014-08-19 | |
dc.identifier.citation | 趙芳筠 (2012) 攝鐵調控蛋白及鐵離子對於腸炎弧菌胞外蛋白酶調控之研究. 碩士論文, 國立台灣大學, 台北市.
林慈玲 (2013) 腸炎弧菌胞外蛋白酶受 Fur 調控及 RyhB 特性分析. 碩士論文, 國立臺灣大學, 台北市. Altuvia, S., A. Zhang, L. Argaman, A. Tiwari and G. Storz (1998). 'The Escherichia coli OxyS regulatory RNA represses fhlA translation by blocking ribosome binding.' EMBO J 17(20): 6069-6075. Bolotin, A., B. Quinquis, A. Sorokin and S. D. Ehrlich (2005). 'Clustered regularly interspaced short palindrome repeats (CRISPRs) have spacers of extrachromosomal origin.' Microbiology 151(Pt 8): 2551-2561. Brinkley, A. W., F. A. Rommel and T. W. Huber (1976). 'The isolation of Vibrio parahaemolyticus and related vibrios from moribund aquarium lobsters.' Can J Microbiol 22(2): 315-317. Broberg, C. A., L. Zhang, H. Gonzalez, M. A. Laskowski-Arce and K. Orth (2010). 'A Vibrio effector protein is an inositol phosphatase and disrupts host cell membrane integrity.' Science 329(5999): 1660-1662. Brouns, S. J., M. M. Jore, M. Lundgren, E. R. Westra, R. J. Slijkhuis, A. P. Snijders, M. J. Dickman, K. S. Makarova, E. V. Koonin and J. van der Oost (2008). 'Small CRISPR RNAs guide antiviral defense in prokaryotes.' Science 321(5891): 960-964. Burdette, D. L., M. L. Yarbrough, A. Orvedahl, C. J. Gilpin and K. Orth (2008). 'Vibrio parahaemolyticus orchestrates a multifaceted host cell infection by induction of autophagy, cell rounding, and then cell lysis.' Proc Natl Acad Sci U S A 105(34): 12497-12502. Charles A. Kaysner, C. A., Jr., Paula A. Trost, June H. Wetherington, Karen C. Jinneman, Walter E. Hill, And Marleen M. Wekell (1994). 'Urea Hydrolysis Can Predict the Potential Pathogenicity of Vibrio parahaemolyticus Strains Isolated in the Pacific Northwest.' Applied and Environmental Microbiology Vol. 60, No. 8. Cornelis, G. R. (2006). 'The type III secretion injectisome.' Nat Rev Microbiol 4(11): 811-825. Davis, B. M., M. Quinones, J. Pratt, Y. Ding and M. K. Waldor (2005). 'Characterization of the small untranslated RNA RyhB and its regulon in Vibrio cholerae.' J Bacteriol 187(12): 4005-4014. Dickenson, N. E., L. Zhang, C. R. Epler, P. R. Adam, W. L. Picking and W. D. Picking (2011). 'Conformational changes in IpaD from Shigella flexneri upon binding bile salts provide insight into the second step of type III secretion.' Biochemistry 50(2): 172-180. Ebralidze, A. K., F. C. Guibal, U. Steidl, P. Zhang, S. Lee, B. Bartholdy, M. A. Jorda, V. Petkova, F. Rosenbauer, G. Huang, T. Dayaram, J. Klupp, K. B. O'Brien, B. Will, M. Hoogenkamp, K. L. Borden, C. Bonifer and D. G. Tenen (2008). 'PU.1 expression is modulated by the balance of functional sense and antisense RNAs regulated by a shared cis-regulatory element.' Genes Dev 22(15): 2085-2092. Geissmann, T. A. and D. Touati (2004). 'Hfq, a new chaperoning role: binding to messenger RNA determines access for small RNA regulator.' EMBO J 23(2): 396-405. Ghildiyal, M. and P. D. Zamore (2009). 'Small silencing RNAs: an expanding universe.' Nat Rev Genet 10(2): 94-108. Gotoh, K., T. Kodama, H. Hiyoshi, K. Izutsu, K. S. Park, R. Dryselius, Y. Akeda, T. Honda and T. Iida (2010). 'Bile acid-induced virulence gene expression of Vibrio parahaemolyticus reveals a novel therapeutic potential for bile acid sequestrants.' PLoS One 5(10): e13365. Gottesman, S. and G. Storz (2011). 'Bacterial small RNA regulators: versatile roles and rapidly evolving variations.' Cold Spring Harb Perspect Biol 3(12). Hansen, T. B., T. I. Jensen, B. H. Clausen, J. B. Bramsen, B. Finsen, C. K. Damgaard and J. Kjems (2013). 'Natural RNA circles function as efficient microRNA sponges.' Nature 495(7441): 384-388. Hiyoshi, H., T. Kodama, K. Saito, K. Gotoh, S. Matsuda, Y. Akeda, T. Honda and T. Iida (2011). 'VopV, an F-actin-binding type III secretion effector, is required for Vibrio parahaemolyticus-induced enterotoxicity.' Cell Host Microbe 10(4): 401-409. Huang, S. H., C. K. Wang, H. L. Peng, C. C. Wu, Y. T. Chen, Y. M. Hong and C. T. Lin (2012). 'Role of the small RNA RyhB in the Fur regulon in mediating the capsular polysaccharide biosynthesis and iron acquisition systems in Klebsiella pneumoniae.' BMC Microbiol 12: 148. Hyatt, D., G.L. Chen, P. F. Locascio, M. L. Land, F. W. Larimer, L. J. Hauser (2010). 'Prodigal: prokaryotic gene recognition and translation initiation site identification.' BMC Bioinformatics. 11(1):119. Hyatt, D., P. F. Locascio, L. J. Hauser, E.C. Uberbacher (2012).'Gene and translation initiation site prediction in metagenomic sequences.' Bioinformatics. 28(17):2223-2230 Izore, T., V. Job and A. Dessen (2011). 'Biogenesis, regulation, and targeting of the type III secretion system.' Structure 19(5): 603-612. Jousselin, A., L. Metzinger and B. Felden (2009). 'On the facultative requirement of the bacterial RNA chaperone, Hfq.' Trends Microbiol 17(9): 399-405. Kaberdin, V. R. and U. Blasi (2006). 'Translation initiation and the fate of bacterial mRNAs.' FEMS Microbiol Rev 30(6): 967-979. Kodama, T., M. Rokuda, K. S. Park, V. V. Cantarelli, S. Matsuda, T. Iida and T. Honda (2007). 'Identification and characterization of VopT, a novel ADP-ribosyltransferase effector protein secreted via the Vibrio parahaemolyticus type III secretion system 2.' Cell Microbiol 9(11): 2598-2609. Krachler, A. M., H. Ham and K. Orth (2011). 'Outer membrane adhesion factor multivalent adhesion molecule 7 initiates host cell binding during infection by gram-negative pathogens.' Proc Natl Acad Sci U S A 108(28): 11614-11619. Krachler, A. M., K. Mende, C. Murray and K. Orth (2012). 'In vitro characterization of multivalent adhesion molecule 7-based inhibition of multidrug-resistant bacteria isolated from wounded military personnel.' Virulence 3(4): 389-399. Krachler, A. M. and K. Orth (2011). 'Functional characterization of the interaction between bacterial adhesin multivalent adhesion molecule 7 (MAM7) protein and its host cell ligands.' J Biol Chem 286(45): 38939-38947. Luong, P., L. N. Kinch, C. A. Brautigam, N. V. Grishin, D. R. Tomchick and K. Orth (2010). 'Kinetic and structural insights into the mechanism of AMPylation by VopS Fic domain.' J Biol Chem 285(26): 20155-20163. Makino, K., K. Oshima, K. Kurokawa, K. Yokoyama, T. Uda, K. Tagomori, Y. Iijima, M. Najima, M. Nakano, A. Yamashita, Y. Kubota, S. Kimura, T. Yasunaga, T. Honda, H. Shinagawa, M. Hattori and T. Iida (2003). 'Genome sequence of Vibrio parahaemolyticus: a pathogenic mechanism distinct from that of V cholerae.' Lancet 361(9359): 743-749. Marraffini, L. A. and E. J. Sontheimer (2008). 'CRISPR interference limits horizontal gene transfer in staphylococci by targeting DNA.' Science 322(5909): 1843-1845. Masse, E., F. E. Escorcia and S. Gottesman (2003). 'Coupled degradation of a small regulatory RNA and its mRNA targets in Escherichia coli.' Genes Dev 17(19): 2374-2383. Masse, E. and S. Gottesman (2002). 'A small RNA regulates the expression of genes involved in iron metabolism in Escherichia coli.' Proc Natl Acad Sci U S A 99(7): 4620-4625. Matsuda, S., T. Kodama, N. Okada, K. Okayama, T. Honda and T. Iida (2010). 'Association of Vibrio parahaemolyticus thermostable direct hemolysin with lipid rafts is essential for cytotoxicity but not hemolytic activity.' Infect Immun 78(2): 603-610. Matsuda, S., N. Okada, T. Kodama, T. Honda and T. Iida (2012). 'A cytotoxic type III secretion effector of Vibrio parahaemolyticus targets vacuolar H+-ATPase subunit c and ruptures host cell lysosomes.' PLoS Pathog 8(7): e1002803. McCarter, L. (1999). 'The multiple identities of Vibrio parahaemolyticus.' J Mol Microbiol Biotechnol 1(1): 51-57. Meador, C. E., M. M. Parsons, C. A. Bopp, P. Gerner-Smidt, J. A. Painter and G. J. Vora (2007). 'Virulence gene- and pandemic group-specific marker profiling of clinical Vibrio parahaemolyticus isolates.' J Clin Microbiol 45(4): 1133-1139. Mey, A. R., S. A. Craig and S. M. Payne (2005). 'Characterization of Vibrio cholerae RyhB: the RyhB regulon and role of ryhB in biofilm formation.' Infect Immun 73(9): 5706-5719. Michael Zuker (2003). ' Mfold web server for nucleic acid folding and hybridization prediction' Nucl. Acids Res. (2003) 31 (13): 3406-3415. Mojica, F. J., C. Diez-Villasenor, J. Garcia-Martinez and E. Soria (2005). 'Intervening sequences of regularly spaced prokaryotic repeats derive from foreign genetic elements.' J Mol Evol 60(2): 174-182. Morita, T., Y. Mochizuki and H. Aiba (2006). 'Translational repression is sufficient for gene silencing by bacterial small noncoding RNAs in the absence of mRNA destruction.' Proc Natl Acad Sci U S A 103(13): 4858-4863. Murphy, E. R. and S. M. Payne (2007). 'RyhB, an iron-responsive small RNA molecule, regulates Shigella dysenteriae virulence.' Infect Immun 75(7): 3470-3477. Oglesby-Sherrouse, A. G. and E. R. Murphy (2013). 'Iron-responsive bacterial small RNAs: variations on a theme.' Metallomics 5(4): 276-286. Oglesby, A. G., E. R. Murphy, V. R. Iyer and S. M. Payne (2005). 'Fur regulates acid resistance in Shigella flexneri via RyhB and ydeP.' Mol Microbiol 58(5): 1354-1367. Ono, T., K. S. Park, M. Ueta, T. Iida and T. Honda (2006). 'Identification of proteins secreted via Vibrio parahaemolyticus type III secretion system 1.' Infect Immun 74(2): 1032-1042. Papenfort, K., V. Pfeiffer, S. Lucchini, A. Sonawane, J. C. Hinton and J. Vogel (2008). 'Systematic deletion of Salmonella small RNA genes identifies CyaR, a conserved CRP-dependent riboregulator of OmpX synthesis.' Mol Microbiol 68(4): 890-906. Payne, S. M., E. E. Wyckoff, E. R. Murphy, A. G. Oglesby, M. L. Boulette and N. M. Davies (2006). 'Iron and pathogenesis of Shigella: iron acquisition in the intracellular environment.' Biometals 19(2): 173-180. Philippe, N., Alcaraz, J. P., Coursange, E., Geiselmann, J., & Schneider, D. (2004). Improvement of pCVD442, a suicide plasmid for gene allele exchange in bacteria. Plasmid, 51(3), 246-225. Pourcel, C., G. Salvignol and G. Vergnaud (2005). 'CRISPR elements in Yersinia pestis acquire new repeats by preferential uptake of bacteriophage DNA, and provide additional tools for evolutionary studies.' Microbiology 151(Pt 3): 653-663. Prevost, K., G. Desnoyers, J. F. Jacques, F. Lavoie and E. Masse (2011). 'Small RNA-induced mRNA degradation achieved through both translation block and activated cleavage.' Genes Dev 25(4): 385-396. Salvail, H. and E. Masse (2012). 'Regulating iron storage and metabolism with RNA: an overview of posttranscriptional controls of intracellular iron homeostasis.' Wiley Interdiscip Rev RNA 3(1): 26-36. Salzman, J., C. Gawad, P. L. Wang, N. Lacayo and P. O. Brown (2012). 'Circular RNAs are the predominant transcript isoform from hundreds of human genes in diverse cell types.' PLoS One 7(2): e30733. Schafer A, Kalinowski J, Simon R, Seep-Feldhaus AH & Puhler A (1990) High-frequency conjugal plasmid transfer from gram-negative Escherichia coli to various gram-positive coryneform bacteria. J Bacteriol 172:1663-1666 Shimohata, T. and A. Takahashi (2010). 'Diarrhea induced by infection of Vibrio parahaemolyticus.' J Med Invest 57(3-4): 179-182. Shinoda, S. (2011). 'Sixty years from the discovery of Vibrio parahaemolyticus and some recollections.' Biocontrol Sci 16(4): 129-137. Su, Y. C. and C. Liu (2007). 'Vibrio parahaemolyticus: a concern of seafood safety.' Food Microbiol 24(6): 549-558. Tanabe, T., T. Funahashi, H. Nakao, J. Maki and S. Yamamoto (2013). 'The Vibrio parahaemolyticus Small RNA RyhB Promotes Production of the Siderophore Vibrioferrin by Stabilizing the Polycistronic mRNA.' J Bacteriol 195(16): 3692-3703. Trosky, J. E., S. Mukherjee, D. L. Burdette, M. Roberts, L. McCarter, R. M. Siegel and K. Orth (2004). 'Inhibition of MAPK signaling pathways by VopA from Vibrio parahaemolyticus.' J Biol Chem 279(50): 51953-51957. V. Solovyev, A Salamov (2011) Automatic Annotation of Microbial Genomes and Metagenomic Sequences. In Metagenomics and its Applications in Agriculture, Biomedicine and Environmental Studies (Ed. R.W. Li), Nova Science Publishers, p. 61-78 Vogel, J., L. Argaman, E. G. Wagner and S. Altuvia (2004). 'The small RNA IstR inhibits synthesis of an SOS-induced toxic peptide.' Curr Biol 14(24): 2271-2276. Vogel, J. and K. Papenfort (2006). 'Small non-coding RNAs and the bacterial outer membrane.' Curr Opin Microbiol 9(6): 605-611. Wassarman, K. M., A. Zhang and G. Storz (1999). 'Small RNAs in Escherichia coli.' Trends Microbiol 7(1): 37-45. Waters, L. S. and G. Storz (2009). 'Regulatory RNAs in bacteria.' Cell 136(4): 615-628. Westra, E. R., P. B. van Erp, T. Kunne, S. P. Wong, R. H. Staals, C. L. Seegers, S. Bollen, M. M. Jore, E. Semenova, K. Severinov, W. M. de Vos, R. T. Dame, R. de Vries, S. J. Brouns and J. van der Oost (2012). 'CRISPR immunity relies on the consecutive binding and degradation of negatively supercoiled invader DNA by Cascade and Cas3.' Mol Cell 46(5): 595-605. Wiedenheft, B., S. H. Sternberg and J. A. Doudna (2012). 'RNA-guided genetic silencing systems in bacteria and archaea.' Nature 482(7385): 331-338. Wilderman, P. J., N. A. Sowa, D. J. FitzGerald, P. C. FitzGerald, S. Gottesman, U. A. Ochsner and M. L. Vasil (2004). 'Identification of tandem duplicate regulatory small RNAs in Pseudomonas aeruginosa involved in iron homeostasis.' Proc Natl Acad Sci U S A 101(26): 9792-9797. Wyckoff, E. E., A. R. Mey and S. M. Payne (2007). 'Iron acquisition in Vibrio cholerae.' Biometals 20(3-4): 405-416. Yanagihara, I., K. Nakahira, T. Yamane, S. Kaieda, K. Mayanagi, D. Hamada, T. Fukui, K. Ohnishi, S. Kajiyama, T. Shimizu, M. Sato, T. Ikegami, M. Ikeguchi, T. Honda and H. Hashimoto (2010). 'Structure and functional characterization of Vibrio parahaemolyticus thermostable direct hemolysin.' J Biol Chem 285(21): 16267-16274. Yeung, P. S. and K. J. Boor (2004). 'Epidemiology, pathogenesis, and prevention of foodborne Vibrio parahaemolyticus infections.' Foodborne Pathog Dis 1(2): 74-88. Zhang, L., A. M. Krachler, C. A. Broberg, Y. Li, H. Mirzaei, C. J. Gilpin and K. Orth (2012). 'Type III effector VopC mediates invasion for Vibrio species.' Cell Rep 1(5): 453-460. Zhou, X., M. E. Konkel and D. R. Call (2010). 'Regulation of type III secretion system 1 gene expression in Vibrio parahaemolyticus is dependent on interactions between ExsA, ExsC, and ExsD.' Virulence 1(4): 260-272. Zhu, Y., M. J. Rowley, G. Bohmdorfer and A. T. Wierzbicki (2013). 'A SWI/SNF chromatin-remodeling complex acts in noncoding RNA-mediated transcriptional silencing.' Mol Cell 49(2): 298-309. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/55827 | - |
dc.description.abstract | RyhB 是一個被廣泛研究的小型核糖核酸,可與標的 mRNA 鹼基配對進行調控目標基因,進而影響細菌生理及致病力。本研究以腸炎弧菌 Vibrio parahaemolyticus no.93 作為實驗菌株,建構腸炎弧菌 ryhB 刪除突變株及補償株,探討腸炎弧菌中,鐵離子相關基因、化學趨化性相關基因、移動性相關基因以及毒性因子相關基因的表現是否亦受到 RyhB 的調控影響。本篇研究證實腸炎弧菌 V. parahamoelyticus no.93 當中之小型核糖核酸 RyhB,經由 qRT-PCR 及北方墨點法分析基因轉錄表現,結果顯示與鐵離子平衡相關的超氧歧化酶 sodB (VP2118)、ferric coprogen 外膜受體 fhuE (VPA0150),化學趨化性相關之趨化性蛋白 cheY (VP2331),移動性相關的鞭毛驅動蛋白 motA (VPA1556)、 motB (VP0690)、σ54-依賴調節因子 lafK (VPA1538)、鞭毛基因 flaD (VP0790)、極性鞭毛蛋白 flaK (VP2253) 及鞭毛蛋白 fliS (VP2254),毒性相關基因中的吸附因子 mam7 (VP1611)、T3SS1 調節子 toxS (VP0819) 及 toxR (VP0820) 皆受到 RyhB 負調控而抑制其表現,iscS (VP0596) 受到 RyhB 正調控而促進其表現,而 rpoS (VP2553) 不受 RyhB 的調控。而於補償株中觀察到 sdhC (VP0843)、vopQ (VP1680)、vopS (VP1686)、VPA0450、phospholipase C (VP1098) 五個基因未能回復其野生株的現象。推測除了 RyhB 外,尚有因子影響基因之表現。本研究證實 RyhB 在腸炎弧菌中扮演的調控角色,除了與鐵離子攝取外,亦與化學趨化性、移動性及 T3SS1 之調控相關。 | zh_TW |
dc.description.abstract | RyhB, one of the well-studied sRNAs, exert their regulatory function by short base pairing with their target mRNAs. In this study, adopting Vibrio parahaemolyticus no. 93 to construct the deletion mutant to investigate iron-related genes, chemotaxis and stress factor genes, motility genes and virulence genes in Vibrio parahaemolyticus. Resulting of qRT-PCR and northern blotting indicate that the iron-related genes of superoxide dismutase sodB (VP2118), outer membrane receptor for ferric coprogen fhuE(VPA0150), chemotaxis-related gene of chemotaxis protein cheY(VP2331), motility-related genes of flagella motor protein motA(VPA1556), motB(VP0690),σ54-dependent regulator lafK (VPA1538), flagellin flaD (VP0790), polar flagellar protein flaK (VP2253) and fliS (VP2254), virulence genes of adhesion factor mam7 (VP1611), type 3 secretion system 1 (T3SS1) regulator toxS (VP0819) and toxR (VP0820) negative regulated by RyhB. iscS (VP0596) positive regulated by RyhB. rpoS (VP2553) is not regulated by RyhB. By observing complementary strain, the result indicate that sdhC (VP0843), vopQ (VP1680), vopS (VP1686),VPA0450 and phospholipase C (VP1098) can not rescue their gene expression in wild type strain, showing that besides RyhB, there is other factor which can effect gene expression. In this study, confirmed that besides of iron-related genes, chemotaxis genes, motility genes and virulence genes also can be regulted by RyhB. | en |
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dc.description.tableofcontents | 誌謝 i
中文摘要 ii 英文摘要 iii 目錄 iv 表次 viii 圖次 ix 縮寫表 xi 壹、前言 1 一、腸炎弧菌 (Vibrio parahaemolyticus) 1 1. 概述 1 2. 性狀 2 3. 毒性因子 2 二、腸炎弧菌毒性因子 4 1. 概述 4 2. 多價黏附分子7 (Mutivalent adhesion molecule 7, MAM7) 4 3. TDH及TRH毒素 5 4. 第三型分泌系統1 ( T3SS1) 6 5. 第三型分泌系統2 (T3SS2) 7 三、微型核醣核酸 (small RNA) 8 1. 概述 8 2. 細菌sRNA調節轉錄因子機制的概念及調節的特性 9 四、調節核糖核酸(Regulatory RNAs) 10 1. 概述 10 2. 長段非編碼核糖核酸 (Long non-coding RNAs, lncRNA) 11 3. 環形核糖核酸(Circular RNAs) 11 4. CRISPR RNA 12 五、RyhB 12 1. 概述 13 2. RyhB的調控與研究 13 3. 弧菌中的RyhB 15 4. 弧菌之外的RyhB的相關研究 16 六、 研究動機與目的 17 貳、實驗材料與方法 18 I.實驗材料 18 一、實驗菌株、引子與質體 18 二、培養基 18 三、藥品與試劑 18 四、各種溶液與緩衝溶液 19 五、儀器設備 22 六、實驗使用套組 23 II.實驗方法 24 一、DNA 技術 24 二、RNA 技術 29 三、建構 ΔryhB 突變株 32 四、建構補償株 34 五、南方墨漬法 35 六、北方墨漬法 37 七、生物資訊分析 39 八、統計分析 40 參、實驗結果 41 一、建構腸炎弧菌 no.93 之 ryhB 刪除突變株 41 二、南方墨點法證實 ryhB 突變株 42 三、比較野生株與突變株生長速率 43 四、可能受 RyhB 調控的目標基因互補序列的預測 43 五、比對可能受 RyhB 調控之基因於不同品系腸炎弧菌間目標基因存在與否及其基因相似性 44 六、以 qRT-PCR 分析目標基因的最大表現時間點 45 七、以 qRT-PCR 分析腸炎弧菌野生株與 ryhB 突變株中目標基因的表現 46 八、以北方墨點法分析腸炎弧菌野生株與 ryhB 突變株目標基因的表現 47 九、建構腸炎弧菌 no. 93 之 ryhB 補償株及 ΔryhB /p 菌株 47 十、比較 WT/p、ΔryhB / p 及補償株 ΔryhB /pryhB 菌株生長速率 48 十一、以 qRT-PCR 分析腸炎弧菌 WT/p、ΔryhB/p 及補償株 ΔryhB/pryhB 可能受 RyhB 調控之目標基因的表現 48 十二、以 qRT-PCR 分析腸炎弧菌 WT/p 及 ryhB 過表現菌株 WT/ pryhB 毒性相關基因的表現 50 肆、討論 51 一、 突變株中所刪除之部分 ryhB 基因序列與 VP0106 基因序列重疊 51 二、 微型核糖核酸調節時與目標基因鹼基配對區域之預測問題 51 三、 腸炎弧菌 no.93 與腸炎弧菌 RIMD2210633 部分序列差異 53 四、腸炎弧菌中 RyhB 調節研究中所探討的目標基因是否需 Hfq 參與 54 五、證實腸炎弧菌中 RyhB 對移動性及毒性相關基因具調控功能 54 伍、結論 56 陸、未來展望 57 柒、參考文獻 58 | |
dc.language.iso | zh-TW | |
dc.title | 腸炎弧菌小型核糖核酸 RyhB 的調控 | zh_TW |
dc.title | Characterization of the regulatory roles of small RNA RyhB in Vibrio parahaemolyticus | en |
dc.type | Thesis | |
dc.date.schoolyear | 102-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 黃憲達(Hsien-Da Huang),詹世鵬(Shih-Peng, CHAN) | |
dc.subject.keyword | 小型核糖核酸,腸炎弧菌, | zh_TW |
dc.subject.keyword | small RNA,RyhB,vibrio parahaemolyticus, | en |
dc.relation.page | 100 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2014-08-19 | |
dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
dc.contributor.author-dept | 農業化學研究所 | zh_TW |
顯示於系所單位: | 農業化學系 |
文件中的檔案:
檔案 | 大小 | 格式 | |
---|---|---|---|
ntu-103-1.pdf 目前未授權公開取用 | 2.23 MB | Adobe PDF |
系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。