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http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/54926完整後設資料紀錄
| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 黃太煌(Tai-Huang Huang) | |
| dc.contributor.author | Shu-Yu Huang | en |
| dc.contributor.author | 黃書毓 | zh_TW |
| dc.date.accessioned | 2021-06-16T03:41:45Z | - |
| dc.date.available | 2018-03-16 | |
| dc.date.copyright | 2015-03-16 | |
| dc.date.issued | 2015 | |
| dc.date.submitted | 2015-02-12 | |
| dc.identifier.citation | References:
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/54926 | - |
| dc.description.abstract | PML protein is mainly present inside the nucleus in the form of PML nuclear bodies (PML-NBs) and serves as a SUMO E3 ligase which facilitates SUMOylation on PML itself and proteins in PML-NBs. PML has a conserved N-terminal TRIM/RBCC region comprised of a RING finger domain, two B-boxes and a coiled-coil region. The purpose of this study is to investigate the structural basis of SUMOylation on PML TRIM domains which is essential in the formation of PML-NBs. However, not much of the structural information of PML is known today. In this work we solved the structures of RING finger and B-box 1 of dimer by advanced NMR techniques. We identified the residues involved in the binding of SUMO E2 enzyme Ubc9 and PML TRIM domains. The result of SPR indicated that dimeric domain RB1 showed much stronger interaction with Ubc9 (KD=16uM) than any single domain (KD=490uM for RING finger and KD=94uM for B-box 1), suggesting that RING finger and B-box 1 bound with Ubc9 in synergy. Here, we provide the structures of PML TRIM domains and the bindings with Ubc9 at molecular level in order to understand the SUMOylation mechanism on PML TRIM domains. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-16T03:41:45Z (GMT). No. of bitstreams: 1 ntu-104-D97b46020-1.pdf: 14668767 bytes, checksum: 0429dd414315a5c23547801c85395ed0 (MD5) Previous issue date: 2015 | en |
| dc.description.tableofcontents | Table of Contents
Verification letter from the Oral Examination Committee i Acknowledgements ii Chinese Abstract iii English Abstract iv List of Figures vii List of Tables viii List of Abbreviations ix Chapter 1: Introduction 1.1 PML and the PML-Nuclear Body 1 1.2 SUMOylation drives PML-NB formation 2 1.3 PML TRIM/RBCC domains 4 Chapter 2: Materials and Methods 2.1 Cloning, Expression, and Protein Purification 8 2.2 NMR Spectroscopy 10 2.3 NMR Structure Determination 11 2.4 Analysis of Protein Dynamics by NMR 12 2.5 Residual dipolar couplings (RDC) measurement 13 2.6 Hydrogen-deuterium exchange experiment 14 2.7 Mapping of protein-protein interaction site by NMR chemical shift perturbation (CSP) 15 2.8 Circular Dichroism (CD) measurement 15 2.9 Surface Plasmon Resonance (SPR) assay 16 2.10 Small Angle X-ray Scattering (SAXS) measurement 16 Chapter 3: Results 3.1 PML Domains Architecture and Sequence Alignment 18 3.2 Solution Structure of RING Finger 20 3.3 Dynamics analysis of RING Finger 24 3.4 Concentration dependence of B-box 1 dimerization 25 3.5 Solution Structure of B-box 1 26 3.6 Dynamics analysis of B-box 1 29 3.7 SAXS for PML Domains and Ubc9 32 3.8 Linker between RING Finger and B-box 1 33 3.9 Mapping the interaction sites of PML domains on Ubc9 35 3.10 Mapping the interaction site of Ubc9 on PML domains 37 3.11 SPR quantification of the Ubc9 binding affinity with PML domains 40 3.12 HADDOCK model of the complex 41 Chapter 4: Discussion 43 Chapter 5: Conclusion and Future Plan 50 References 94 Publications | |
| dc.language.iso | en | |
| dc.subject | SUMO | zh_TW |
| dc.subject | PML | zh_TW |
| dc.subject | TRIM/RBCC | zh_TW |
| dc.subject | RING finger | zh_TW |
| dc.subject | B-box 1 | zh_TW |
| dc.title | 前骨髓性細胞白血病蛋白的結構和分子交互作用機制 | zh_TW |
| dc.title | Structural Characterization and Molecular Interaction with the TRIM Domains of Promyelocytic Leukemia Protein | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 103-1 | |
| dc.description.degree | 博士 | |
| dc.contributor.coadvisor | 陳瑞華(Ruey-Hwa Chen) | |
| dc.contributor.oralexamcommittee | 施修明(Hsiu-Ming Shih),陳金榜(Chin-Pan Chen),蘇士哲(Shih-Che Sue) | |
| dc.subject.keyword | PML,TRIM/RBCC,RING finger,B-box 1,SUMO, | zh_TW |
| dc.relation.page | 99 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2015-02-12 | |
| dc.contributor.author-college | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 生化科學研究所 | zh_TW |
| 顯示於系所單位： | 生化科學研究所 | |
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