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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 林劭品(Shau-Ping Lin) | |
dc.contributor.author | Chung-Hao Lu | en |
dc.contributor.author | 呂仲浩 | zh_TW |
dc.date.accessioned | 2021-05-15T18:00:30Z | - |
dc.date.available | 2014-01-27 | |
dc.date.available | 2021-05-15T18:00:30Z | - |
dc.date.copyright | 2014-01-27 | |
dc.date.issued | 2014 | |
dc.date.submitted | 2014-01-16 | |
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dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/5490 | - |
dc.description.abstract | 絲胺酸蛋白酶抑制蛋白E2(SERPINE2)為一種可以抑制絲胺酸蛋白酶活性的蛋白質,其藉由抑制絲胺酸蛋白酶活性來參與許多生理功能。目前已知SERPINE2可以調控plasminogen activator及thrombin的酵素活性。在第一部份的研究中,首先我們從小鼠儲精囊分泌液中純化出具有可以抑制plasminogen activator活性的SERPINE2蛋白質。接著發現SERPINE2會結合在射出的小鼠精子以及已經游入輸卵管的精子,但是無法在已發生獲能反應的精子身上發現SERPINE2蛋白質的存在。此外,在體外試驗中發現SERPINE2也會抑制牛血清白蛋白(BSA)所引起的精子獲能反應,使得精子無法與卵子結合進而影響與卵子的受精作用。精子身上的膽固醇移除(cholesterol efflux)為發生獲能反應的重要步驟,而SERPINE2的存在會去抑制精子身上膽固醇的移除,因此SERPINE2可能扮演著精子去獲能因子(decapacitation factor)的角色。
Plasminogen activator已被證實存在於排卵過程中的濾泡壁上,但其在卵子成熟過程中的相關功能仍然未知。在第二部份的研究中我們證實了PLAU (urokinase plasminogen activator)及其抑制者SERPINE2參與小鼠卵丘細胞擴張(cumulus expansion)及卵子成熟。當高量的SERPINE2結合上卵丘細胞之細胞間質(ECM)時會降低PLAU的活性,導致卵丘細胞無法擴張,進而影響卵子成熟。因此在病人進行人工生殖技術過程中,額外加入PLAU蛋白質於體外培養系統中,或許可以幫助不成熟卵子進行最後階段之熟成而提高受精率。 綜合這些實驗結果,我們認為SERPINE2蛋白質可以在精子到達輸卵管之前保護精子並避免其過早發生獲能反應。此外,我們推測部分不孕症病人之卵丘細胞無法擴張及卵子無法成熟可能與SERPINE2蛋白質不正常的高量表現有關。 | zh_TW |
dc.description.abstract | SERPINE2, one of the potent serine protease inhibitors that modulate the activity of plasminogen activator and thrombin, is implicated in many biological processes. In the first study, we purified SERPINE2 from mouse seminal vesicle secretion based on its potent inhibitory activity against the urokinase-type plasminogen activator. A prominent amount of SERPINE2 was detected on ejaculated and oviductal spermatozoa, predominantly on uncapacitated sperm, suggesting the need to remove SERPINE2 before initiation of the capacitation process. Moreover, SERPINE2 could inhibit in vitro bovine serum albumin-induced sperm capacitation and prevent sperm binding to the egg, thus blocking fertilization. It acts through preventing cholesterol efflux, one of the initiation events of capacitation, from the sperm. These findings suggest that SERPINE2 protein may play a role as a sperm decapacitation factor.
Plasminogen activators play a crucial role in follicle wall rupture during ovulation; however, their function in oocyte maturation during pre-ovulation remained unclear. Our second study provides the first evidence that PLAU (urokinase plasminogen activator) and its inhibitor SERPINE2 are involved in murine cumulus expansion and oocyte maturation. High SERPINE2 levels bound to the extracellular matrix of cumulus cells could reduce PLAU activity, and ultimately suppressing cumulus expansion and oocyte maturation. PLAU supplementation to culture medium may assist the final maturation of the immature human oocytes collected during assisted reproductive technology procedures, thus providing a potential therapeutic strategy. Based on these results, SERPINE2 protein possibly influencese on sperm to prevent precocious capacitation and the acrosome reaction before sperm reach the oviduct. In addition, we suppose that aberrantly high SERPINE2 protein levels in cumulus cells may be one of the etiologies for patients with defects in cumulus expansion and subsequent oocyte maturation. | en |
dc.description.provenance | Made available in DSpace on 2021-05-15T18:00:30Z (GMT). No. of bitstreams: 1 ntu-103-D95642001-1.pdf: 18995982 bytes, checksum: ba8baf63e380aef036acc877fa404d4e (MD5) Previous issue date: 2014 | en |
dc.description.tableofcontents | 口試委員審定書…………………………………………………………………………2
誌謝………………………………………………………………………3 中文摘……………………………………………………………………4 Abstract………………………………………………………………6 Chapter 1 Introduction……………………………………………………………15 1.1 Prolegomenon………………………………………………………15 1.2 Sperm Capacitation / Decapacitation and Fertility.....................................15 1.3 Identification and Characterization of Seminal Plasma Secretions Importance for Decapacitation factor…………………………………………………………………17 1.4 SERPINE2 Protein Characteristics and Functions…………………………………19 1.5 The Potential Role of SERPINE2 in Reproduction……………………………………20 1.6 Cumulus Expansion and Oocyte Maturation…………………………………21 Chapter 2 Study I: SERPINE2, a Serine Protease Inhibitor Extensively Expressed in Adult Male Mouse Reproductive Tissues, May Serve as a Murine Sperm Decapacitation Factor………………………………24 2.1 Introduction…………………………………………………………………………………24 2.2 Materials and Methods……………………………………………………………………25 2.3 Results........................................37 2.4 Discussion……………………………………………………………………………………44 2.5 Conclusion………………………………………………………………49 2.6 Figures…………………………………………………………………51 Chapter 3 Study II: Involvement of the serine protease inhibitor, SERPINE2, and the urokinase plasminogen activator in cumulus expansion and oocyte maturation………………………………………………………………60 3.1 Introduction……………………………………………………………60 3.2 Materials and Methods................................61 3.3 Results…………………………………………………………………70 3.4 Discussion………………………………………………………………77 3.5 Conclusion................................82 3.6 Figures…………………………………………………………………84 Chapter 4 Summary…………………………………………………………………114 Chapter 5 Future prospects………………………………………………………………116 References……………………………………………………………118 Curriculum Vitae……………………………………………………………………130 | |
dc.language.iso | en | |
dc.title | 絲胺酸蛋白酶抑制蛋白E2在精子獲能作用與卵子成熟過程中所扮演的角色 | zh_TW |
dc.title | The roles of the SERPINE2 protein in sperm capacitation and oocyte maturation | en |
dc.type | Thesis | |
dc.date.schoolyear | 102-1 | |
dc.description.degree | 博士 | |
dc.contributor.oralexamcommittee | 李勝祥(Sheng-Hsiang Li),胡玉銘(Yuh-MingHwu),宋麗英(Li-Ying Sung),羅清維(Ching-Wei Luo),林翰佳(Han-Jia Lin) | |
dc.subject.keyword | 絲胺酸蛋白?抑制蛋白E2,絲胺酸蛋白?,精子,獲能作用,受精作用,卵丘細胞擴張,卵子成熟, | zh_TW |
dc.subject.keyword | SERPINE2,serine protease,sperm,capacitation,fertilization,plasminogen activator,cumulus expansion,oocyte maturation, | en |
dc.relation.page | 132 | |
dc.rights.note | 同意授權(全球公開) | |
dc.date.accepted | 2014-01-17 | |
dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
dc.contributor.author-dept | 生物科技研究所 | zh_TW |
顯示於系所單位: | 生物科技研究所 |
文件中的檔案:
檔案 | 大小 | 格式 | |
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ntu-103-1.pdf | 18.55 MB | Adobe PDF | 檢視/開啟 |
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