請用此 Handle URI 來引用此文件:
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/54640
完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 常怡雍(Yee-Yung Charng) | |
dc.contributor.author | Tzu-Ying Yang | en |
dc.contributor.author | 楊子瑩 | zh_TW |
dc.date.accessioned | 2021-06-16T03:36:07Z | - |
dc.date.available | 2022-08-03 | |
dc.date.copyright | 2020-08-06 | |
dc.date.issued | 2020 | |
dc.date.submitted | 2020-08-03 | |
dc.identifier.citation | Ahn SG, Thiele DJ (2003) Redox regulation of mammalian heat shock factor 1 is essential for Hsp gene activation and protection from stress. Genes Dev. 17: 516-528 Alscher RG (1989) Biosynthesis and antioxidant function of glutathione in plants. Physiol. Plant. 77: 457-464 Ball L, Accotto GP, Bechtold U, Creissen G, Funck D, Jimenez A, Kular B, Leyland N, Mejia-Carranza J, Reynolds H, Karpinski S, Mullineaux PM (2004) Evidence for a direct link between glutathione biosynthesis and stress defense gene expression in Arabidopsis. Plant Cell 16: 2448-2462 Bangash SaK, Muller-Schussele SJ, Solbach D, Jansen M, Fiorani F, Schwarzlander M, Kopriva S, Meyer AJ (2019) Low-glutathione mutants are impaired in growth but do not show an increased sensitivity to moderate water deficit. PLOS ONE 14: e0220589 Bedhomme M, Adamo M, Marchand CH, Couturier J, Rouhier N, Lemaire SD, Zaffagnini M, Trost P (2012) Glutathionylation of cytosolic glyceraldehyde-3-phosphate dehydrogenase from the model plant Arabidopsis thaliana is reversed by both glutaredoxins and thioredoxins in vitro. Biochem. J. 445: 337-347 Boyer JS (1982) Plant productivity and environment. Science 218: 443-448 Cairns NG, Pasternak M, Wachter A, Cobbett CS, Meyer AJ (2006) Maturation of arabidopsis seeds is dependent on glutathione biosynthesis within the embryo. Plant Physiol. 141: 446-455 Cairns NG, Pasternak M, Wachter A, Cobbett CS, Meyer AJ (2006) Maturation of Arabidopsis seeds is dependent on glutathione biosynthesis within the embryo. Plant Physiol. 141: 446-455 Charng Y-Y, Liu H-C, Liu N-Y, Hsu F-C, Ko S-S (2006) Arabidopsis Hsa32, a novel heat shock protein, is essential for acquired thermotolerance during long recovery after acclimation. Plant Physiol. 140: 1297-1305 Charng YY, Liu HC, Liu NY, Hsu FC, Ko SS (2006) Arabidopsis Hsa32, a novel heat shock protein, is essential for acquired thermotolerance during long recovery after acclimation. Plant Physiol. 140: 1297-1305 Cheng MC, Ko K, Chang WL, Kuo WC, Chen GH, Lin TP (2015) Increased glutathione contributes to stress tolerance and global translational changes in Arabidopsis. Plant J. 83: 926-939 Chi WT, Fung RW, Liu HC, Hsu CC, Charng YY (2009) Temperature-induced lipocalin is required for basal and acquired thermotolerance in Arabidopsis. Plant, Cell Environ. 32: 917-927 Cobbett CS, May MJ, Howden R, Rolls B (1998) The glutathione‐deficient, cadmium‐sensitive mutant, cad2–1, of Arabidopsis thaliana is deficient in γ‐glutamylcysteine synthetase. Plant J. 16: 73-78 Doyle SM, Wickner S (2009) Hsp104 and ClpB: protein disaggregating machines. Trends Biochem. Sci. 34: 40-48 Drazkiewicz M, Skorzynska-Polit E, Krupa Z (2010) Effect of BSO-supplemented heavy metals on antioxidant enzymes in Arabidopsis thaliana. Ecotoxicol. Environ. Saf. 73: 1362-1369 Dron M, Clouse SD, Dixon RA, Lawton MA, Lamb CJ (1988) Glutathione and fungal elicitor regulation of a plant defense gene promoter in electroporated protoplasts. Proc. Natl. Acad. Sci. U.S.A. 85: 6738-6742 Dubreuil-Maurizi C, Vitecek J, Marty L, Branciard L, Frettinger P, Wendehenne D, Meyer AJ, Mauch F, Poinssot B (2011) Glutathione deficiency of the Arabidopsis mutant pad2-1 affects oxidative stress-related events, defense gene expression, and the hypersensitive response. Plant Physiol. 157: 2000-2012 Foyer CH, Halliwell B (1976) The presence of glutathione and glutathione reductase in chloroplasts: a proposed role in ascorbic acid metabolism. Planta 133: 21-25 Foyer CH, Lopez‐Delgado H, Dat JF, Scott IM (1997) Hydrogen peroxide‐and glutathione‐associated mechanisms of acclimatory stress tolerance and signalling. Physiol. Plant. 100: 241-254 Fratelli M, Gianazza E, Ghezzi P (2004) Redox proteomics: identification and functional role of glutathionylated proteins. Expert Rev. Proteomics 1: 365-376 Gálvez S, Bismuth E, Sarda C, Gadal P (1994) Purification and characterization of chloroplastic NADP-isocitrate dehydrogenase from mixotrophic tobacco cells (comparison with the cytosolic isoenzyme). Plant Physiol. 105: 593-600 Geu-Flores F, Moldrup ME, Bottcher C, Olsen CE, Scheel D, Halkier BA (2011) Cytosolic gamma-glutamyl peptidases process glutathione conjugates in the biosynthesis of glucosinolates and camalexin in Arabidopsis. Plant Cell 23: 2456-2469 Giustarini D, Rossi R, Milzani A, Colombo R, Dalle‐Donne I (2004) S‐glutathionylation: from redox regulation of protein functions to human diseases. J. Cell. Mol. Med. 8: 201-212 Glover JR, Lindquist S (1998) Hsp104, Hsp70, and Hsp40: a novel chaperone system that rescues previously aggregated proteins. Cell 94: 73-82 Hodges M (2002) Enzyme redundancy and the importance of 2‐oxoglutarate in plant ammonium assimilation. J. Exp. Bot. 53: 905-916 Holmgren A (1976) Hydrogen donor system for Escherichia coli ribonucleoside-diphosphate reductase dependent upon glutathione. Proc. Natl. Acad. Sci. U.S.A. 73: 2275-2279 Hoppe G, Chai YC, Crabb JW, Sears J (2004) Protein S-glutathionylation in retinal pigment epithelium converts heat shock protein 70 to an active chaperone. Exp. Eye Res. 78: 1085-1092 Horvath E, Bela K, Holinka B, Riyazuddin R, Galle A, Hajnal A, Hurton A, Feher A, Csiszar J (2019) The Arabidopsis glutathione transferases, AtGSTF8 and AtGSTU19 are involved in the maintenance of root redox homeostasis affecting meristem size and salt stress sensitivity. Plant Sci. 283: 366-374 Hu C, Lin SY, Chi WT, Charng YY (2012) Recent gene duplication and subfunctionalization produced a mitochondrial GrpE, the nucleotide exchange factor of the Hsp70 complex, specialized in thermotolerance to chronic heat stress in Arabidopsis. Plant Physiol. 158: 747-758 Ito H, Iwabuchi M, Ogawa KI (2003) The sugar-metabolic enzymes aldolase and triose-phosphate isomerase are targets of glutathionylation in Arabidopsis thaliana: detection using biotinylated glutathione. Plant Cell Physiol. 44: 655-660 Jiang K, Moe-Lange J, Hennet L, Feldman LJ (2016) Salt stress affects the redox status of Arabidopsis root meristems. Front. Plant Sci. 7: 81 Krishna P, Gloor G (2001) The Hsp90 family of proteins in Arabidopsis thaliana. Cell Stress Chaperones 6: 238 Kumar D, Chattopadhyay S (2018) Glutathione modulates the expression of heat shock proteins via the transcription factors BZIP10 and MYB21 in Arabidopsis. J. Exp. Bot. 69: 3729-3743 Lancien M, Gadal P, Hodges M (1998) Molecular characterization of higher plant NAD‐dependent isocitrate dehydrogenase: evidence for a heteromeric structure by the complementation of yeast mutants. Plant J. 16: 325-333 Lancien M, Gadal P, Hodges M (2000) Enzyme redundancy and the importance of 2-oxoglutarate in higher plant ammonium assimilation. Plant Physiol. 123: 817-824 Lee U, Rioflorido I, Hong SW, Larkindale J, Waters ER, Vierling E (2007) The Arabidopsis ClpB/Hsp100 family of proteins: chaperones for stress and chloroplast development. Plant J. 49: 115-127 Lemaitre T, Hodges M (2006) Expression analysis of Arabidopsis thaliana NAD-dependent isocitrate dehydrogenase genes shows the presence of a functional subunit that is mainly expressed in the pollen and absent from vegetative organs. Plant Cell Physiol. 47: 634-643 Lemaitre T, Hodges M (2006) Expression analysis of Arabidopsis thaliana NAD-dependent isocitrate dehydrogenase genes shows the presence of a functional subunit that is mainly expressed in the pollen and absent from vegetative organs. Plant Cell Physiol. 47: 634-643 Lemaitre T, Urbanczyk-Wochniak E, Flesch V, Bismuth E, Fernie AR, Hodges M (2007) NAD-dependent isocitrate dehydrogenase mutants of Arabidopsis suggest the enzyme is not limiting for nitrogen assimilation. Plant Physiol. 144: 1546-1558 Lemaitre T, Urbanczyk-Wochniak E, Flesch V, Bismuth E, Fernie AR, Hodges M (2007) NAD-dependent isocitrate dehydrogenase mutants of Arabidopsis suggest the enzyme is not limiting for nitrogen assimilation. Plant Physiol. 144: 1546-1558 Lin M, Behal RH, Oliver DJ (2004) Characterization of a mutation in the IDH-II subunit of the NAD+-dependent isocitrate dehydrogenase from Arabidopsis thaliana. Plant Sci. 166: 983-988 Lind C, Gerdes R, Hamnell Y, Schuppe-Koistinen I, Von Löwenhielm HB, Holmgren A, Cotgreave IA (2002) Identification of S-glutathionylated cellular proteins during oxidative stress and constitutive metabolism by affinity purification and proteomic analysis. Arch. Biochem. Biophys. 406: 229-240 Lindquist S (1986) The heat-shock response. Annu. Rev. Biochem. 55: 1151-1191 Lindquist S, Craig EA (1988) The heat-shock proteins. Annu. Rev. Genet. 22: 631-677 Liu HC, Liao HT, Charng YY (2011) The role of class A1 heat shock factors (HSFA1s) in response to heat and other stresses in Arabidopsis. Plant, Cell Environ. 34: 738-751 Liu Y, Zhang C, Chen J, Guo L, Li X, Li W, Yu Z, Deng J, Zhang P, Zhang K, Zhang L (2013) Arabidopsis heat shock factor HsfA1a directly senses heat stress, pH changes, and hydrogen peroxide via the engagement of redox state. Plant Physiol. Biochem. 64: 92-98 Marty L, Siala W, Schwarzlander M, Fricker MD, Wirtz M, Sweetlove LJ, Meyer Y, Meyer AJ, Reichheld JP, Hell R (2009) The NADPH-dependent thioredoxin system constitutes a functional backup for cytosolic glutathione reductase in Arabidopsis. Proc. Natl. Acad. Sci. U.S.A. 106: 9109-9114 Masella R, Di Benedetto R, Vari R, Filesi C, Giovannini C (2005) Novel mechanisms of natural antioxidant compounds in biological systems: involvement of glutathione and glutathione-related enzymes. J. Nutr. Biochem. 16: 577-586 Mcloughlin F, Kim M, Marshall RS, Vierstra RD, Vierling E (2019) HSP101 interacts with the proteasome and promotes the clearance of ubiquitylated protein aggregates. Plant Physiol. 180: 1829-1847 Meyer AJ, Brach T, Marty L, Kreye S, Rouhier N, Jacquot JP, Hell R (2007) Redox-sensitive GFP in Arabidopsis thaliana is a quantitative biosensor for the redox potential of the cellular glutathione redox buffer. Plant J. 52: 973-986 Miseta A, Csutora P (2000) Relationship between the occurrence of cysteine in proteins and the complexity of organisms. Mol. Biol. Evol. 17: 1232-1239 Mitchell JB, Russo A, Kinsella TJ, Glatstein E (1983) Glutathione elevation during thermotolerance induction and thermosensitization by glutathione depletion. Cancer Res. 43: 987-991 Niazi AK, Bariat L, Riondet C, Carapito C, Mhamdi A, Noctor G, Reichheld JP (2019) Cytosolic isocitrate dehydrogenase from Arabidopsis thaliana Is regulated by glutathionylation. Antioxidants 8: 16-33 Noctor G, Arisi A-CM, Jouanin L, Kunert KJ, Rennenberg H, Foyer CH (1998) Glutathione: biosynthesis, metabolism and relationship to stress tolerance explored in transformed plants. J. Exp. Bot. 49: 623-647 Noctor G, Foyer CH (1998) Ascorbate and glutathione: keeping active oxygen under control. Annu. Rev. Plant Biol. 49: 249-279 Noctor G, Mhamdi A, Chaouch S, Han Y, Neukermans J, Marquez‐Garcia B, Queval G, Foyer CH (2012) Glutathione in plants: an integrated overview. Plant, Cell Environ. 35: 454-484 Parisy V, Poinssot B, Owsianowski L, Buchala A, Glazebrook J, Mauch F (2007) Identification of PAD2 as a gamma-glutamylcysteine synthetase highlights the importance of glutathione in disease resistance of Arabidopsis. Plant J. 49: 159-172 Parisy V, Poinssot B, Owsianowski L, Buchala A, Glazebrook J, Mauch F (2007) Identification of PAD2 as a γ‐glutamylcysteine synthetase highlights the importance of glutathione in disease resistance of Arabidopsis. Plant J. 49: 159-172 Pasternak M, Lim B, Wirtz M, Hell R, Cobbett CS, Meyer AJ (2008) Restricting glutathione biosynthesis to the cytosol is sufficient for normal plant development. Plant J. 53: 999-1012 Reichheld JP, Khafif M, Riondet C, Droux M, Bonnard G, Meyer Y (2007) Inactivation of thioredoxin reductases reveals a complex interplay between thioredoxin and glutathione pathways in Arabidopsis development. Plant Cell 19: 1851-1865 Requejo R, Hurd TR, Costa NJ, Murphy MP (2010) Cysteine residues exposed on protein surfaces are the dominant intramitochondrial thiol and may protect against oxidative damage. FEBS J. 277: 1465-1480 Russo A, Mitchell J, Mcpherson S (1984) The effects of glutathione depletion on thermotolerance and heat stress protein synthesis. Br. J. Cancer 49: 753-758 Schlaeppi K, Bodenhausen N, Buchala A, Mauch F, Reymond P (2008) The glutathione-deficient mutant pad2-1 accumulates lower amounts of glucosinolates and is more susceptible to the insect herbivore Spodoptera littoralis. Plant J. 55: 774-786 Schmidtmann E, König A-C, Orwat A, Leister D, Hartl M, Finkemeier I (2014) Redox regulation of Arabidopsis mitochondrial citrate synthase. Mol. Plant Pathol. 7: 156-169 Shanmugam V, Tsednee M, Yeh KC (2012) ZINC TOLERANCE INDUCED BY IRON 1 reveals the importance of glutathione in the cross-homeostasis between zinc and iron in Arabidopsis thaliana. Plant J. 69: 1006-1017 Thordal‐Christensen H, Zhang Z, Wei Y, Collinge DB (1997) Subcellular localization of H2O2 in plants. H2O2 accumulation in papillae and hypersensitive response during the barley—powdery mildew interaction. Plant J. 11: 1187-1194 Townsend DM (2007) S-glutathionylation: indicator of cell stress and regulator of the unfolded protein response. Mol. Interventions 7: 313-324 Vernoux T, Wilson RC, Seeley KA, Reichheld J-P, Muroy S, Brown S, Maughan SC, Cobbett CS, Van Montagu M, Inzé D (2000) The ROOT MERISTEMLESS1/CADMIUM SENSITIVE2 gene defines a glutathione-dependent pathway involved in initiation and maintenance of cell division during postembryonic root development. Plant Cell 12: 97-109 Vierling E (1991) The roles of heat shock proteins in plants. Annu. Rev. Plant Physiol. Plant Mol. Biol. 42: 579-620 Volkov RA, Panchuk, Ii, Mullineaux PM, Schoffl F (2006) Heat stress-induced H2O2 is required for effective expression of heat shock genes in Arabidopsis. Plant Mol. Biol. 61: 733-746 Wang W, Vinocur B, Shoseyov O, Altman A (2004) Role of plant heat-shock proteins and molecular chaperones in the abiotic stress response. Trends Plant Sci. 9: 244-252 Wani R, Nagata A, Murray BW (2014) Protein redox chemistry: post-translational cysteine modifications that regulate signal transduction and drug pharmacology. Front. Pharmacol. 5: 224 Wingate VP, Lawton MA, Lamb CJ (1988) Glutathione causes a massive and selective induction of plant defense genes. Plant Physiol. 87: 206-210 Wray W, Boulikas T, Wray VP, Hancock R (1981) Silver staining of proteins in polyacrylamide gels. Anal. Biochem. 118: 197-203 Wu TY, Juan YT, Hsu YH, Wu SH, Liao HT, Fung RW, Charng YY (2013) Interplay between heat shock proteins HSP101 and HSA32 prolongs heat acclimation memory posttranscriptionally in Arabidopsis. Plant Physiol. 161: 2075-2084 Xiang C, Oliver DJ (1998) Glutathione metabolic genes coordinately respond to heavy metals and jasmonic acid in Arabidopsis. Plant Cell 10: 1539-1550 Yadav S (2010) Heavy metals toxicity in plants: an overview on the role of glutathione and phytochelatins in heavy metal stress tolerance of plants. S. Afr. J. Bot. 76: 167-179 Yang J, Zhang H, Gong W, Liu Z, Wu H, Hu W, Chen X, Wang L, Wu S, Chen C, Perrett S (2020) S-Glutathionylation of human inducible Hsp70 reveals a regulatory mechanism involving the C-terminal alpha-helical lid. J. Biol. Chem. 295: 8302-8324 Yeh CH, Kaplinsky NJ, Hu C, Charng YY (2012) Some like it hot, some like it warm: phenotyping to explore thermotolerance diversity. Plant Sci. 195: 10-23 Yoshida K, Hisabori T (2014) Mitochondrial isocitrate dehydrogenase is inactivated upon oxidation and reactivated by thioredoxin-dependent reduction in Arabidopsis. Front. Environ. Sci. 2 Zaffagnini M, Bedhomme M, Marchand CH, Morisse S, Trost P, Lemaire SD (2012) Redox regulation in photosynthetic organisms: focus on glutathionylation. Antioxid. Redox Signal. 16: 567-586 Zaffagnini M, Michelet L, Marchand C, Sparla F, Decottignies P, Le Marechal P, Miginiac-Maslow M, Noctor G, Trost P, Lemaire SD (2007) The thioredoxin-independent isoform of chloroplastic glyceraldehyde-3-phosphate dehydrogenase is selectively regulated by glutathionylation. FEBS J. 274: 212-226 Zhang H, Yang J, Wu S, Gong W, Chen C, Perrett S (2016) Glutathionylation of the bacterial Hsp70 chaperone DnaK provides a link between oxidative stress and the heat shock response. J. Biol. Chem. 291: 6967-6981 | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/54640 | - |
dc.description.abstract | 穀胱甘肽 (Glutathione) 在細胞中扮演著維持氧化還原恆定的重要角色。先前研究發現,經過 buthionine sulfoximine 處理後的動物細胞,其穀胱甘肽的含量減少,也讓耐熱性和主要的熱休克蛋白的合成受到抑制。為了了解穀胱甘肽的含量是否會影響植物的耐熱性和熱休克反應,此研究對穀胱甘肽合成相關的阿拉伯芥突變種 (cad2-1、pad2-1和zir1) 進行了四種不同的耐熱性測定。所有突變種在四種熱處理條件下皆顯示了熱敏感的表現型,說明了穀胱甘肽對於植物耐熱的重要性。在正常條件和熱處理後,突變種中的穀胱甘肽和氧化型穀胱甘肽 (oxidized glutathione) 的含量有顯著地降低。外加 10 mM 的穀胱甘肽使得pad2-1 恢復部分的耐熱性。在熱處理前後,熱休克蛋白 HSP101、HSP90、HSP70、sHSP-CI、HSA32 和 HSFA2 的表現量並不會受到穀胱甘肽含量影響。蛋白質沈澱分離分析發現造成 pad2-1 的熱敏感性的原因和 hsp101 是有所不同的,暗示穀胱甘肽的缺乏不至於導致分子伴侶功能的喪失。在突變種中,NAD+-異檸檬酸脫氫酶的活性有些微但顯著的下降,顯示穀胱甘肽的含量可能會影響某些氧化還原調節蛋白的功能。因此,在植物和動物中,穀胱甘肽對於熱休克反應之調節機制並不相同。結果也顯示穀胱甘肽在阿拉伯芥中有著抵抗熱逆境的重要作用。 | zh_TW |
dc.description.abstract | Glutathione (GSH) is an important metabolite that functions in maintaining the cellular redox homeostasis in response to stresses. The reduction of glutathione content upon buthionine sulfoximine treatment results in the inhibition of thermotolerance acquisition and major heat shock proteins (HSPs) synthesis during heat acclimation in animal cells. To investigate whether the level of glutathione influences the thermotolerance and heat shock response in plants, the glutathione deficiency mutants (cad2-1, pad2-1, and zir1) in Arabidopsis were subjected to four distinct thermotolerance assay. All the mutants showed heat-sensitive phenotypes, indicating that glutathione is vital for thermotolerance. Analysis of the GSH/GSSG (oxidized glutathione) content by ultra-performance liquid chromatography mass spectrometry showed that the GSH/GSSG levels were dramatically reduced in the mutants under both normal and heat stress conditions. The thermotolerance of pad2-1 can be partially restored under heat stress conditions by treating with exogenous glutathione at 10 mM. Quantitative RT-PCR and western blotting analysis showed that the abundance of heat-inducible proteins, i.e. HSP101, HSP90, HSP70, sHSP-CI, HSA32, and HSFA2, were not significantly affected in the mutants before and after heat treatment. The thermo-sensitivity of pad2-1 was not due to compromised proteostasis, suggesting that glutathione deficiency does not substantially affect chaperone functions. The activity of NAD+-dependent isocitrate dehydrogenase decreased slightly but significantly in the mutants, indicating that the glutathione content may influence function of some redox-regulated proteins. Hence, the mechanisms of glutathione-mediated heat stress response in plants and animals may not be the same. Glutathione plays a pivotal role in Arabidopsis in conferring heat tolerance. | en |
dc.description.provenance | Made available in DSpace on 2021-06-16T03:36:07Z (GMT). No. of bitstreams: 1 U0001-0208202023052300.pdf: 3959269 bytes, checksum: 8fc99289f960ec159f239b84db62d336 (MD5) Previous issue date: 2020 | en |
dc.description.tableofcontents | Contents 謝誌 -------------------------------------------------------------------------------------------------i 摘要 -------------------------------------------------------------------------------------------------ii Abstract -------------------------------------------------------------------------------------------iii Abbreviations -------------------------------------------------------------------------------------v Contents ------------------------------------------------------------------------------------------vii Chapter 1 Introduction ---------------------------------------------------------------------1 1.1 Plant heat shock response --------------------------------------------------------------1 1.2 Glutathione in plants --------------------------------------------------------------------2 1.3 Glutathione content and abiotic stresses ---------------------------------------------4 1.4 The function of protein glutathionylation --------------------------------------------6 1.5 NAD+-dependent isocitrate dehydrogenase (IDH) ---------------------------------8 1.6 Specific aims -----------------------------------------------------------------------------9 Chapter 2 Materials and Methods ------------------------------------------------------10 2.1 Plant materials and growth conditions ----------------------------------------------10 2.2 Genomic DNA extraction, genotyping, and DNA sequencing -------------------10 2.3 Quantification of glutathione contents and UPLC-MS/MS analysis -----------12 2.4 Thermotolerance assays ---------------------------------------------------------------13 2.5 Glutathione treatment of seedlings --------------------------------------------------14 2.6 Isolation of total RNA and quantitative RT-PCR analysis ------------------------14 2.7 Protein extraction and immunoblotting ---------------------------------------------15 2.8 DAB staining ---------------------------------------------------------------------------15 2.9 Separation of soluble and insoluble protein fractions ----------------------------16 2.10 Silver staining -------------------------------------------------------------------------18 2.11 Crude protein extraction and NAD+-Isocitrate dehydrogenase (IDH) activity assay ------------------------------------------------------------------------------------19 Chapter 3 Results ----------------------------------------------------------------------------20 3.1 Confirmation of GSH1 mutant genotypes ------------------------------------------20 3.2 The content of glutathione in response to heat stress -----------------------------20 3.3 Glutathione is vital for thermotolerance in Arabidopsis --------------------------21 3.4 Exogenous glutathione supplement increases heat stress tolerance -------------22 3.5 HS response is not affected by depletion of glutathione --------------------------23 3.6 HS-induced accumulation of H2O2 was not altered by glutathione depletion -23 3.7 Thermotolerance defect of pad2-1 is not due to protein aggregation -----------24 3.8 Reduction of NAD+-dependent isocitrate dehydrogenase activity in pad2-1 after HS ---------------------------------------------------------------------------------25 Chapter 4 Discussion ------------------------------------------------------------------------27 4.1 Overestimated levels of GSSG in Arabidopsis seedlings -------------------------27 4.2 Reduced glutathione level does not affect the expression of major HSPs in Arabidopsis -----------------------------------------------------------------------------27 4.3 The effects of H2O2 accumulation during long recovery time -------------------28 4.4 Glutathione deficiency may not affect proteostasis during HS ------------------28 4.5 Reduced activity of NAD+-dependent isocitrate dehydrogenase under SAT assay condition ------------------------------------------------------------------------29 Chapter 5 Future work ----------------------------------------------------------------------30 Figures --------------------------------------------------------------------------------------------32 Appendix ------------------------------------------------------------------------------------------45 References ----------------------------------------------------------------------------------------48 | |
dc.language.iso | en | |
dc.title | 穀胱甘肽在阿拉伯芥耐熱性反應中的作用 | zh_TW |
dc.title | The Role of Glutathione in Thermotolerance and Heat Shock Response in Arabidopsis thaliana | en |
dc.type | Thesis | |
dc.date.schoolyear | 108-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 葉國楨(Kuo-Chen Yeh),楊健志(Chien-Chih Yang) | |
dc.subject.keyword | 熱逆境反應,耐熱性,穀胱甘肽,γ-谷氨酰半胱氨酸合成酶,阿拉伯芥, | zh_TW |
dc.subject.keyword | Heat stress response,thermotolerance,glutathione,γ-glutamylcysteine synthetase,Arabidopsis thaliana, | en |
dc.relation.page | 54 | |
dc.identifier.doi | 10.6342/NTU202002237 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2020-08-04 | |
dc.contributor.author-college | 生命科學院 | zh_TW |
dc.contributor.author-dept | 生化科技學系 | zh_TW |
顯示於系所單位: | 生化科技學系 |
文件中的檔案:
檔案 | 大小 | 格式 | |
---|---|---|---|
U0001-0208202023052300.pdf 目前未授權公開取用 | 3.87 MB | Adobe PDF |
系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。