利用聚對二甲苯製備可調控之光學及生物功能性新型人工水晶體

Abstract

本研究中，展示了一款透過化學氣相沉積封裝法(chemical vapor deposition encapsulation)，合成功能性聚對二甲苯(functionalized poly-p-xylylenes)並包覆液體所製備的新型人工水晶體(intraocular lens, IOL)，此新型裝置同時提供了可調控的光學參數及生物功能性。作為一卓越的光學裝置，此新型人工水晶體裝置擁有高折射率，其來自於聚對二甲苯高分子之特性，而透過控制包覆液體的潤濕性(wettability)，可賦予此裝置可調控的有效焦距(effective focal length)，此裝置更能提供抗紫外光之特性；而作為一重要之生醫裝置，此新型人工水晶體展現良好的生物相容性，且由於聚對二甲苯高分子之特性，此裝置經過Alizarin red staining、掃描式電子顯微鏡(scanning electron microscope)與X光能量散布分析儀(energy dispersive X-ray spectroscopy)等驗證，證實可有效減少手術後由裝置引發的鈣沉積(postoperative calcification)，此外，利用功能性聚對二甲苯表面的化學特性，可準確地在此人工水晶體表面之特定位置修飾功能性生物分子，在本研究中，使用了具有增進細胞貼附的RGD胜肽分子與擁有抗結垢效果的聚乙二醇分子(poly(ethylene glycol))進行新型人工水晶體之表面改質，並使用人類晶體上皮細胞(human lens epithelial cells)、纖維母細胞(fibroblast cells)與人類角膜上皮細胞(human corneal epithelial cells)驗證其功效，結果顯示此技術能有效地在特定區域增進或抑制細胞的貼附，對於避免由裝置引起的併發症有十分重要之意義。

An innovative intraocular lens (IOL) device fabricated based on chemical vapor deposition encapsulation process by using functionalized poly-p-xylylenes is introduced herein. The advanced device provides non-compromised design parameters for both its optical and biological properties. As an excellent optical device, it provides a high refractive index that stems from intrinsic poly-p-xylylenes and a tunable effective focal length that is realized by manipulating the wetting properties of the encapsulated liquids; the device also offers protection from UV irradiation. As a key medical device, it exhibits excellent biocompatibility and reduced postoperative calcification through the intrinsic properties of poly-p-xylylenes after conducting alizarin red staining, scanning electron microscope, and energy dispersive X-ray spectroscopy examinations. In addition, these synergic functions, cell-adhering RGD peptide and anti-fouling poly(ethylene glycol) molecules, are provided with precise surface chemistry for location to a guided attachment or repellent properties for human lens epithelial cells, fibroblast cells, and human corneal epithelial cells, which is important in preventing device-associated complications.