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| ???org.dspace.app.webui.jsptag.ItemTag.dcfield??? | Value | Language |
|---|---|---|
| dc.contributor.advisor | 廖文亮 | |
| dc.contributor.author | Tung-Chou Hsieh | en |
| dc.contributor.author | 謝東洲 | zh_TW |
| dc.date.accessioned | 2021-06-16T02:44:46Z | - |
| dc.date.available | 2025-05-17 | |
| dc.date.copyright | 2015-07-29 | |
| dc.date.issued | 2015 | |
| dc.date.submitted | 2015-07-20 | |
| dc.identifier.citation | 大洋，2014。飼料中添加守宮木對於點带石斑魚成長及免疫方面影響。國
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/54208 | - |
| dc.description.abstract | 本論文研究目的為藉由體內投餵實驗，探討在飼料中添加北蟲草培養米基萃取物對於點帶石斑魚在成長及非特異性免疫方面之影響。實驗分別為餵食在飼料中添加以甲醇、酒精、醋酸乙酯和水，萃取的培養米基萃取物以及無添加和直接添加培養米基之組別。成長方面檢測增重率(Percentage weight gain, PWG)及飼料利用率(Feed efficiency, FE)，免疫方面檢測項目包括超氧陰離子產生率(Superoxide anion production ratio)和溶菌酶活性(Lysozyme activity)。並分析血清中鹼性磷酸酶(Alkaline phosphatase)、膽固醇(Cholesterol)及三酸甘油脂(Triglyceride)。
實驗使用平均約9.61±1.12克的點帶石斑魚，每組12尾進行三重複，實驗開始21天後測量體重及成長狀況，飼養35天後秤重採樣。結果顯示實驗35天後，魚體增重率介於187%~215%，飼料效率介於100%~113%。非特異性免疫超氧陰離子(O2-)產生率及溶菌酶活性都以在飼料中添加0.5%北蟲草培養米基酒精萃取物組表現高，鹼性磷酸酶以甲醇萃取物組表現最高，直接添加培養米基組及添加培養米基萃取物組在膽固醇及三酸甘油脂都較控制組低。 綜合本研究結果發現，點帶石斑魚連續攝食添加北蟲草培養米基萃取物的飼料35天，對其成長無顯著影響 (p>0.05)。免疫方面結果顯示在飼料中添加北蟲草培養米基萃取物，其白血球超氧陰離子產生率，溶菌酶活性等非特異性免疫指標皆有提升的效果。 | zh_TW |
| dc.description.abstract | This study used an in vivo experiment to investigate the effect of Cordyceps militaris rice medium extract on growth and non-specific immune parameters of grouper (Epinephelus coioides).
Experiments were conducted by adding C. militaris rice medium and C. militaris rice medium extraction, extracted using methanol, ethanol, ethyl acetate and water, to basal diets and administering to grouper for 35 days. Grouper with a body weight of 9.61±1.12g were randomly distributed into tanks in triplicates (at a density of 12 fish/tank). The results showed that final body weight, percentage weight gain and feed efficiency of grouper were not significantly effected when fed with diets containing various extractions of C. militaris rice medium. The results for immune parameters show that the production ability of superoxide anion and lysozyme enzyme activity of plasma are higher in fish fed with diets supplemented by ethanol extracted C. militaris rice medium. Alkaline phosphatase activity is higher in groupers fed with methanol extracted C. militaris rice medium diets. Groupers fed with C. militaris rice medium and C. militaris rice medium extraction have lower cholesterol (Cho) and triglyceride (TG) compared to those fed with control diets. Thus, this study indicated that administration of grouper with C. militaris rice medium extraction supplemented diets has no negative growth impact and can enhance its non-specific immune response. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-16T02:44:46Z (GMT). No. of bitstreams: 1 ntu-104-R02b45014-1.pdf: 1088208 bytes, checksum: 01d69076cc5d42f6aa0b2e523bb5d867 (MD5) Previous issue date: 2015 | en |
| dc.description.tableofcontents | 中文摘要 I
Abstract II 目錄 III 表目錄 VI 圖目錄 VII 第一章 前言 1 第二章 文獻整理 3 2.1.點帶石斑 3 2.2.生態習性 3 2.3.養殖現況 4 2.4.疾病 4 2.5.北蟲草的介紹 5 2.5.1.分佈及型態 5 2.5.2.藥理功能 6 2.5.3.培養情況 6 2.6.免疫刺激物 7 2.7.魚類之免疫系統 7 2.8.血液性狀 9 2.9.研究動機與目的 9 第三章 材料與方法 10 3.1.條件與設計 10 3.2.實驗用添加物 10 3.3.飼料置配過程 11 3.4.一般成分分析 11 3.4.1.水分 11 3.4.2.灰分 11 3.4.3.粗蛋白 11 3.4.4.粗脂質 12 3.5.成長分析 12 3.6.免疫分析 13 3.6.1.血液、血清、頭腎及肝臟採集 13 3.6.2.白血球分離 13 3.6.3. 超氧陰離子(O2-)測定 14 3.6.4. 溶菌酶活性測定 14 3.7.血液生化分析 15 3.7.1.三酸甘油脂 ( Triglyceride, TG )測定 15 3.7.2.膽固醇 ( Cholesterol, Cho )測定 15 3.7.3.鹼性磷酸酶 ( Alkaline phosphatase, ALP ) 15 3.8.統計分析 15 第四章 結果 16 4.1.飼料組成分析 16 4.2.成長表現 16 4.3.非特異性免疫 16 4.4.血脂分析 17 第五章 討論 18 5.1.飼料配方 18 5.2.飼養結果 18 5.3.免疫效果 19 5.4.血液性狀 21 結論 22 參考文獻 23 | |
| dc.language.iso | zh-TW | |
| dc.subject | 萃取 | zh_TW |
| dc.subject | 北蟲草培養米基 | zh_TW |
| dc.subject | 點帶石斑 | zh_TW |
| dc.subject | 成長 | zh_TW |
| dc.subject | 免疫 | zh_TW |
| dc.subject | 北蟲草培養米基 | zh_TW |
| dc.subject | 萃取 | zh_TW |
| dc.subject | 點帶石斑 | zh_TW |
| dc.subject | 成長 | zh_TW |
| dc.subject | 免疫 | zh_TW |
| dc.subject | growth | en |
| dc.subject | Cordyceps militaris rice medium extraction | en |
| dc.subject | Orange-spotted grouper | en |
| dc.subject | non-specific immune response | en |
| dc.subject | growth | en |
| dc.subject | Orange-spotted grouper | en |
| dc.subject | Cordyceps militaris rice medium extraction | en |
| dc.subject | non-specific immune response | en |
| dc.title | 飼料中添加北蟲草培養米基萃取物對點帶石斑成長及免疫能力之影響 | zh_TW |
| dc.title | Effects of dietary Cordyceps militaris rice medium extraction on the growth and immune responses in orange-spotted grouper (Epinephelus coioides) | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 103-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 葉信利,丁雲源,張繼堯 | |
| dc.subject.keyword | 北蟲草培養米基,萃取,點帶石斑,成長,免疫, | zh_TW |
| dc.subject.keyword | Cordyceps militaris rice medium extraction,Orange-spotted grouper,growth,non-specific immune response, | en |
| dc.relation.page | 42 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2015-07-20 | |
| dc.contributor.author-college | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 漁業科學研究所 | zh_TW |
| Appears in Collections: | 漁業科學研究所 | |
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| File | Size | Format | |
|---|---|---|---|
| ntu-104-1.pdf Restricted Access | 1.06 MB | Adobe PDF |
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